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Citations for Promega :
1 - 50 of 1197 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 3’-UTR derived from pNL1.1[Nluc] vector (Promega), and 50 nt poly(A ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected with the Piggybac plasmid plus transposase at a 3:1 ratio using Fugene HD (Promega) and selected with G418 (300 µg/mL ...
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bioRxiv - Biophysics 2022Quote: ... and PiggyBac transposon plasmid containing puromycin selection using FuGENE (Promega, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... and the interferon stimulation response element (ISRE) (Promega), were also transfected into the cells ...
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bioRxiv - Cell Biology 2024Quote: ... cells were co-transfected with pPB vectors and a plasmid encoding hyperactive piggyBac transposase by Viafect (Promega) as described previously (11) ...
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bioRxiv - Physiology 2023Quote: ... the oligo with NF-κB consensus binding element (Promega) was end-labeled by T4 polynucleotide kinase (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... H9 hESC harbouring the mitochondrial matrix mCherry-GFP flux reporter were generated by transfection of 1×105 cells with 1μg pAC150-PiggyBac-matrix-mCherry-eGFPXL (Harper’s lab) and 1μg pCMV-HypBAC-PiggyBac-Helper(Sanger Institute) in conjunction with the transfection reagent FuGENE HD (Promega). The cells were selected and maintained in TeSR™-E8™ medium supplemented with 200 mg/ml Hygromycin and Hygromycin was kept in the medium during differentiation to iNeurons ...
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bioRxiv - Biochemistry 2023Quote: ... Zc3h18-3F-mAID mES cells were transfected with pB-MYC-mZC3H18x-BSD vectors along with a piggyBAC transposase expressing vector (pBase) in a 1:1 ratio using Viafect (Promega). Cell pools were selected with BSD for ∼ 7-10 days or until negative control cells no longer survived ...
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bioRxiv - Molecular Biology 2024Quote: ... MSCs expressing piggyBAC-MLL4-PrLD-HaloTag-Cry2 were stained with 200nM of 646 Janelia Fluor® HaloTag® (Promega; GA1120) diluted in complete culture medium for 20 minutes at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Wildtype mES cells were transfected with pB[MYC-] or pB[MYC-mTurbo-PHAX] vectors along with a piggyBAC transposase expressing vector (pBase) in a 1:1 ratio using Viafect (Promega). Cell pools were selected for successful integration of pB plasmids with Blastocidin (BSD ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected at > 80% confluence with all-in-one PiggyBac plasmids containing different Cas13d orthologs using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 15,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx or DjCas13d using ViaFect™ Transfection Reagent (E4981, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected at > 80% confluence with all-in-one PiggyBac plasmids containing different Cas13d orthologs using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 15,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx or DjCas13d using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... were co-transfected with pMK459 (dTAG-BromoTag-mAID-EGFP-NLS) and pCMV-hyBase (PiggyBac transposase) using ViaFect Transfection reagent (Promega, #E498A) and Opti-MEM reduced serum medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The HMEC cell lines stably expressing doxycycline-inducible Rb variants were generated by transfecting cells with 1 µg of doxycycline- inducible plasmid and 1 µg of PiggyBac Transposase plasmid using the FuGene HD reagent (Promega E2311). Zeocin (300 µg/ml ...
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bioRxiv - Cell Biology 2020Quote: All Luciferase vectors were derived from PGL3-basic vector (Promega, E1751) by Seamless Cloning ...
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bioRxiv - Genomics 2023Quote: ... minCMV (27) and minP (derived from pGL4 (Promega, Madison, WI, USA)) ...
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bioRxiv - Genetics 2022Quote: ... Full-length L1 elements were then cloned into pGEMT Easy Vector (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and rabbit anti-Caspase-3 polyclonal antibody (1:250, Promega/Fisher, PR-G7481) overnight at 4 °C ...
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bioRxiv - Genetics 2024Quote: ... regulatory element activity was measured using the Dual-Luciferase Reporter Assay (Promega, E1910) and a GloMax 96 Luminometer according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... minCMV,33 minHBG,34 or minP (derived from pGL4 (Promega, Madison, WI, USA)) ...
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bioRxiv - Biochemistry 2021Quote: The heat shock element (HSE) firefly luciferase reporter was purchased from Promega (Madison, WI) and has two copies of the HSE promoter driving the luc2P gene ...
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bioRxiv - Microbiology 2024Quote: ... an IFN stimulated response element firefly luciferase reporter plasmid pGL4.45 [luc2P/ISRE/Hygro] (Promega), a gamma activated sequence firefly luciferase reporter plasmid (pGL4[luc2P/GAS RE Hygro] (Promega) ...
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bioRxiv - Biochemistry 2023Quote: N-terminal large (LgBit) and C-terminal small (SmBit) fragments derived from NanoLuc (Promega) were fused to the C-terminus of B56δ and inserted downstream of its N-arm at residue 103 ...
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bioRxiv - Cancer Biology 2023Quote: ... Reference DNA for the analysis was derived from multiple healthy and anonymous male donors (Promega). The labeling and hybridization steps were conducted in accordance with the provided instructions using the SureTag DNA Labelling kit (Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: The Lep-derived constructs were assayed using the TNT T7 Quick Coupled System (#L1170, Promega). Each reaction containing 1 µL of PCR product ...
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bioRxiv - Genomics 2024Quote: ... [59] followed by a 1,653 nt firefly luciferase reporter sequence derived from plasmid pGL4.10 (Promega). Similarly ...
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bioRxiv - Genomics 2023Quote: ... This TP53 response element is controlling the GADD45A gene (7) and is commercially available (pGL4.38, Promega). The benchmark response element was also linked to the three selected spacer sequences and the two core promoters ...
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bioRxiv - Cell Biology 2023Quote: ... Promoter elements used in luciferase reporter expression analyses were cloned into the pGL3-basic vector (Promega).
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bioRxiv - Cancer Biology 2024Quote: The HSF1 activity reporter contains multiple heat shock element (HSE) motifs driving firefly luciferase (Promega #E3751). Experiments were performed with co-transfection of a constitutively active renilla reporter (Addgene #27163 ...
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bioRxiv - Genetics 2024Quote: RhoA activity was assessed using the Serum Response Factor Response Element (SRF-RE) pGL4.34 (Promega, E1350), which drives the transcription of the luciferase reporter gene luc2P in response to activation of Serum Response Factor that triggers RhoA GTPase ...
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bioRxiv - Cell Biology 2022Quote: ... After 3 days Caspase-Glo 3/7 (Promega) and CellTiterGlo (Promega ...
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bioRxiv - Plant Biology 2020Quote: ... Pooled RNA samples derived from each conditions were subjected to DNaseI digest (RNase-free DNaseI, Promega) in the presence of RNase inhibitor (RNasin Plus ...
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bioRxiv - Systems Biology 2020Quote: ... Firefly reporter vector backbone was derived from a commercial vector pGL4.13 with luc2 firefly gene (Promega). HindIII and BglII restriction enzymes (Khan et al. ...
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bioRxiv - Molecular Biology 2023Quote: The pSV-Rluc renilla luciferase internal standard vector was derived from psiCHECK-2 (Promega, catalog # C8021) by digestion with EagI to remove the firefly luciferase gene ...
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bioRxiv - Biochemistry 2023Quote: ... synthetic gene fragments containing response elements were inserted into a pGL4.23[luc2/minP] vector obtained from Promega using KpnI and HindIII (for SRE ...
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bioRxiv - Molecular Biology 2024Quote: ... For the analysis of expression of MERVL elements the RNA was additionally digested with RQ1 DNAse (Promega) to guarantee the absence of contaminant genomic DNA ...
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bioRxiv - Microbiology 2023Quote: ... 5’-CGCCTTCGTTACAAGCATCG-3’; antisense, 5’-AAGAGCAAACGCATCTCCGA-3’), GAPDH (sense, 5’-ACCCACTCCTCCACCTTTGAC-3’; antisense, 5’-CTGTTGCTGTAGCCAAATTCGT-3’) using Green GoTaq master mix (Promega, Madison, WI) with C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmid pGL4.32 containing a firefly luciferase cassette under NF-κB response elements was obtained from Promega (Cat# E8491). For overexpression of ubiquitin ...
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bioRxiv - Genetics 2021Quote: ... this vector was re-cut at the NheI site and the transcriptional insulator element from pGL4.23 (Promega, E8411) was inserted using Gibson Assembly (78) ...
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bioRxiv - Cancer Biology 2023Quote: ... Conserved Elements (CE) were PCR-amplified from GB genomic DNA using GoTaq® G2 DNA Polymerase (Promega, #M7845) (primer sequences listed in Supplementary Table S1 ...
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bioRxiv - Biochemistry 2024Quote: ... GSK-3 (Promega), CK1 (Promega) ...
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bioRxiv - Systems Biology 2020Quote: Renilla control plasmid (3630 bp) was derived from another commercial vector pGL4.70 with the hRluc renilla gene (Promega) by insertion of a moderate-strength P transposase (pTran ...
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bioRxiv - Cell Biology 2021Quote: A probe corresponding to the final 1078 bp of rps6ka3a (RSK2a; NM_212786.1) was generated by cloning a PCR-derived cDNA fragment into in pGEMT-Easy (Promega), linearising with ApaI (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... and splice acceptor (SA)) were derived from the chimeric intron included in the pCI mammalian expression vector (Promega). We used a P2A sequence to prematurely truncate the SEED-target and facilitate expression of a transgene payload ...
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bioRxiv - Microbiology 2023Quote: ... After 30 minutes of incubation at 37°C in 5% CO2 we added 50,000 ADCC-RL cells (Jurkat cell line expressing luciferase gene under the control of the NFAT response element and stably expressing human FcγRIIIa V158; Promega) in 50 µl RPMI 1640 medium with 6% low-IgG serum (Promega) ...
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bioRxiv - Cell Biology 2023Quote: The Foxo1/3/4 responsive luciferase reporter plasmid was constructed by inserting six FoxO REs (FoxO-responsive elements) into the pGL4.23 (Promega). The following sequence was used for the FoxO RE ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting Cckbr1 expression construct was transiently transfected into Hela cells together with a luciferase reporter vector containing cis-acting elements responsive to cAMP (pGL4.29; Promega), Ca2+-dependent nuclear factor of activated T-cells (NFAT ...
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bioRxiv - Genetics 2024Quote: ... Existing plasmids constructs of variant-centered test elements for the five variants of interest (see Dataset S1 for genomic regions) cloned in the pGL4.23 vector (Promega) were used as the initial template in PCR ...