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Citations for Promega :
151 - 200 of 292 citations for PhotoCol Methacrylated Type I Collagen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: Full-length wild-type and mutant Csde1 5’UTRs were inserted pGL3 (Promega, E1751) plasmid in between EcoRI and NcoI sites upstream of Firefly luciferase gene ...
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bioRxiv - Genetics 2022Quote: Type-1 quantitative bioluminescence resonance energy transfer (qBRET) experiments utilised the NanoBRET platform (Promega) and followed the general type-1 qBRET protocol reported by Felce et al.25 The assay was performed in HEK293 cells transiently expressing POPDC1 and POPDC2 constructs which possessed a C-terminal NanoLuc luciferase or HaloTag domains ...
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bioRxiv - Microbiology 2021Quote: ... An on-column DNase I step was added using RQ1 DNase (Promega). Total RNAs were used as templates for SYBR green-based one-step reverse-transcriptase quantitative PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... contaminating DNA was removed by digestion with RNase-free DNase I (Promega). Purified RNAs were again cleaned up using Agencourt RNAClean XP beads as above and eluted into 30 μL H2O ...
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bioRxiv - Genetics 2019Quote: ... RNA samples were DNase I-treated with 1U of RQ1 DNase (Promega) per μg of RNA and purified through a second RNeasy column ...
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bioRxiv - Neuroscience 2021Quote: ... Equivalent amounts of RNA (1 µg) were treated with DNase I (Promega) at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: ... DNA was digested by treating with RQ1 Dnase I (Promega, Madison, WI) and samples were checked for genomic DNA contamination by PCR to detect rplU ...
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bioRxiv - Physiology 2021Quote: ... according to the manufacturer’s instructions and subsequently treated with DNase I (Promega). cDNA was generated using the RevertAid First Strand cDNA Synthesis kit according to manufacturer’s instructions (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... each RNA sample was incubated with RNase-free DNase I (Promega, USA) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Both mouse brain and cell lysates were digested with DNase I (Promega) for 5 minutes at 37°C and then with RNase A (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... The isolated RNA was treated with 10 units of DNase I (Promega), extracted with acidic phenol-chloroform ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated RNA was treated with 10 units of DNase I (Promega), extracted with acidic phenol-chloroform ...
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bioRxiv - Cell Biology 2023Quote: ... Residual DNA was removed by treatment with DNase I (Q1 DNAse, Promega) for 1h at 37 °C (2 units per sample ...
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bioRxiv - Genomics 2019Quote: ... Residual genomic DNA was digested by DNase I treatment (Promega, Cat No-M6101) and 200ng of RNA was reverse transcribed using AMV Reverse Transcriptase (Promega ...
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bioRxiv - Genomics 2022Quote: ... Each 5 μL fraction sample was treated with DNase I (cat #M6101, Promega) for 1 h at 37°C and then diluted 1:20 in 50 μg/mL yeast tRNA (cat #Am7119 ...
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bioRxiv - Developmental Biology 2022Quote: ... After removing the residual genomic DNA with the DNase I Kit (Promega, M6101), 500 ng of total RNA was reverse-transcribed into cDNAs using the PrimeScript RT Reagent Kit (TaKaRa ...
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bioRxiv - Genomics 2021Quote: ... followed by treatment with RNase-free DNase I (Cat. no. M6101, Promega, WI), according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... followed by adding 200◻U of RQI RNase-free DNase I (Promega) for 1◻min ...
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bioRxiv - Molecular Biology 2022Quote: ... Approximately 0.5 μg of RNAs were then digested by DNAse I (Promega, M6101) at 37°C for 50 min to remove the genomic DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... and 2 micrograms from each sample were DNAse I treated (Promega, Madison, WI) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... pCI Luc contains firefly luciferase cDNA cloned MluI / Xba I in pCI (Promega). pNLENG1-ES-IRES (a gift of D.N ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNA samples were treated with DNase I (Promega, Madison, WI, USA) and purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... on-membrane DNase I digestion was performed according to the manufacturer’s protocol (Promega). The integrity of the total RNA was assessed using spectrophotometric analysis using NanoDrop 2000/2000c ...
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bioRxiv - Plant Biology 2024Quote: ... and DNase I was added to digest the genomic DNA (Promega, no. Z3100) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... DNAse digestion was performed using RQ1 DNase I and RQ1 buffer (Promega M610A) for 30min at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Pellets were reconstituted in RNAse-free water and treated with DNase-I (Promega ##) as per manufacturers’ instructions ...
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bioRxiv - Biophysics 2020Quote: CosM6 cells were transfected with ∼1 µg of wild type or mutant constructs using FuGENE6 (Promega) and were patched within 1-2 days after transfection ...
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bioRxiv - Synthetic Biology 2019Quote: The wild type and mutant promoter of miR-146a-5p were constructed into pGL3 vector (Promega). The pGL3-WT or pGL3-mut were co-transfected with pRL-TK using lipofectamine® 3000 ...
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bioRxiv - Biochemistry 2019Quote: Wild-type and mutant AP2ΔµC complexes were expressed in BL21 (DE3) pLysS strain (Promega, Madison, WI) by induction at 23 °C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... wild type or mutant 3′-UTR of DKK3 was cloned into the psicheck-2 vector (Promega). HCT116 cells were transfected with and wild-type or mutant 3′-UTR-luc by using Lipofectamine 3000 ...
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bioRxiv - Molecular Biology 2023Quote: ... The wild-type and mutants of MCHR1 and MCHR2 were constructed into the pcDNA6.0 vector (Promega) for functional assays.
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bioRxiv - Biochemistry 2022Quote: ... to 100 µl add 15 µl DNase I and 1.5 µl RNASin Plus (Promega) before use) ...
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bioRxiv - Systems Biology 2022Quote: ... and RNA (1.5μg) was treated with RQ1 Dnase I (Promega; 1 U/µg RNA). cDNA was synthesized with a qPCRBIO high-quality cDNA synthesis kit ...
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bioRxiv - Genomics 2021Quote: ... Total nucleic acid was then treated with DNase I as recommended (20 units; Promega), re-extracted with phenol/Sevag ...
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bioRxiv - Genomics 2021Quote: ... DNA collected from the DNase I digestion was quantified using a Quantus Fluorometer (Promega), and 10 ng was used to generate the libraries ...
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bioRxiv - Microbiology 2020Quote: One μg of total RNA was treated with 0.4 units of DNase I (Promega) and 0.2 units of Turbo-DNase (Ambion ...
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bioRxiv - Molecular Biology 2022Quote: ... SYBR Green I nucleic acid gel stain and Go Taq Hot Start Polymerase (Promega). qRT-PCRs were carried out in a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Gel extracted products were ligated into the pGEM-T Easy Vector System I (Promega). Ligation products were transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... Extracted RNA was treated with DNase (RQ1 RNase-free DNase I, Promega, Beijing, China) to remove traces of genomic DNA ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was obtained from 1 μg of total RNA pretreated with DNase I (Promega) by reverse transcription reaction using ImProm-II(TM ...
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bioRxiv - Cell Biology 2020Quote: DNA was extracted from each cell type with the Wizard Genomic DNA Purification Kit (Promega, Southampton, UK) and send for STR profiling (Core Genomic Facility ...
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bioRxiv - Plant Biology 2020Quote: ... oryzae (Pso)-treated 9-WT wild type and hac701 with Maxwell 16 LEV Plant RNA Kit (Promega) run on the Maxwell 16 Instrument (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... The transfection of wild-type MCF10A cells and the mutated derivatives was carried out with FuGene (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was isolated from 4-day-old wild-type seedlings using a Maxwell RSC Plant Kit (Promega). cDNA was synthesized from total RNA (100 ng ...
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bioRxiv - Molecular Biology 2020Quote: ... Resuspended grindate was incubated (4°C, 20 min) with 660 U of DNase I (Promega). Insoluble cell debris was pelleted by spinning (16,000 g ...
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bioRxiv - Cell Biology 2021Quote: ... treated with 3 units/sample of DNase I for 2 hours (Promega, Madison, WI, USA), and 1-3μl aliquots were taken for reverse transcription (RT ...
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bioRxiv - Biochemistry 2021Quote: ... 1x protease inhibitor mix (Benzamidine & PMSF)) supplemented with 7 U/ml DNase I (M610A, Promega) and lysed by Dounce homogenization and incubation on ice for 30 min ...
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bioRxiv - Genomics 2019Quote: ... which were subsequently ligated to pGEM-T Vector (pGEM-T Vector System I, Promega, USA), and transformed to DH5α competent cells (Z-Competent E ...
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bioRxiv - Genomics 2021Quote: HDAC activity assays were performed using the HDAC GLO I/II assay kit (Promega, G6430) in 96 well plates following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... smegmatis Complex I (8 μg) was digested with MS-grade trypsin/Lys-C mix (Promega) at a 25:1 (w/w Complex I:protease ratio in 25 mM Tris-HCl ...