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Citations for Promega :
51 - 100 of 877 citations for Peptide T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and pGEM®T Easy vector (Promega) were used for cloning procedures ...
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bioRxiv - Genetics 2020Quote: ... Plasmid vector pGEMT®-T (Promega, USA) was used to clone the amplified DNA fragments containing the 5’ends of cDNA ...
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bioRxiv - Developmental Biology 2019Quote: ... cloned them into pGEM-T vector (Promega), and sequenced them ...
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bioRxiv - Cell Biology 2020Quote: ... inserted into the pGEM-T vector (Promega) and subcloned into pMiRGlo.
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bioRxiv - Developmental Biology 2022Quote: ... Amplicons were cloned into pGEM-T (Promega) using manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... ligated into pGEM T-Easy vector (Promega), and transformed into DH5α cells (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... and cloned into pGEM-T-Easy (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... and pGEM®-T Easy vector (Promega) were used for cloning procedures ...
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bioRxiv - Developmental Biology 2023Quote: ... Using TA cloning (Promega, “Easy-T” Cloning), the resulting amplicons were cloned into th pGEM vector (Promega) ...
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bioRxiv - Developmental Biology 2023Quote: ... or pGEM-T vector system (Promega®), and sequences were validated by Sanger sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... into the pGEM-T vector (Promega A1360). The dCdk8-EGFP rescue construct ...
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bioRxiv - Plant Biology 2024Quote: ... and cloned into pGEM-T easy (Promega) vector ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated and digested into peptides with trypsin (Promega). The resulting peptides were desalted and concentrated before mass spectrometry by the STAGE-TIP method using a C18 resin disk (3M Empore) ...
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bioRxiv - Zoology 2020Quote: After digestion into short peptides by trypsin (Promega), the samples were analyzed using a DiNa nanoLC system (KYA Technologies ...
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bioRxiv - Biochemistry 2022Quote: LC-MS/MS Peptide Reference Mix (V7491, Promega) in HeLa digest (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were eluted by on-bead trypsin (Promega) digest and acidified for MS analysis ...
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bioRxiv - Immunology 2022Quote: ... alkylated and digested into peptides using trypsin (Promega). Peptides were desalted and concentrated using Empore-C18 StageTips and eluted with 0.5% (v/v ...
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bioRxiv - Immunology 2021Quote: ... followed by three washes with TBS-T and 1 hr incubation in TBS-T + 1% milk + anti-mouse serum (1:10,000) (Promega). All steps were carried out at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The PCR product was cloned into a T Easy plasmid using the pGEM®-T Easy Vector (Promega, USA), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: Well-amplified fragments were cloned into a pGEM-T vector using the pGEM-T Easy Vector System (Promega A1360), following the manufacturer’s instructions and bacteria were cultured on LB-agar plates supplemented with ampicillin (150 ug/ml) ...
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bioRxiv - Cell Biology 2019Quote: ... 5’ GA CTC GAG GTT GCA CCT AGT GGA T 3’ The PCR product was first cloned into pGEM-T easy vector (Promega), verified by sequencing and subsequently cloned into pGEX5.1 vector using the EcoRI and XhoI sites ...
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bioRxiv - Genetics 2020Quote: Via T/A ligation the fragment was inserted in the pGEM-T vector following the manufactures recommendations (Promega, Fitchburg, USA). Competent Escherichia coli cells (E ...
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bioRxiv - Microbiology 2022Quote: ... at 72° C for 10 min to add 3’-A overhangs for T/A cloning with pGEM-T Easy plasmid (Promega). A 610 bp DNA fragment upstream of flgV was amplified using PCR primers 85 and 86 ...
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bioRxiv - Molecular Biology 2022Quote: ... REBOV Vp40- pGEM-T Easy and MERS-CoV UpE-pGEM-T Easy were first linearized by Sal I enzyme (Promega) creating a 5’overhang ...
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bioRxiv - Immunology 2023Quote: RNA from purified splenic CD8 T cells was reverse transcribed into cDNA using oligo-d(T) primers and M-MuLV reverse transcription (Promega). Real-time quantitative PCR was performed using SYBR DNA polymerase (Thermo Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... cloned into pGEM®-T easy vector (Promega). Five clones for each variety were sequenced and aligned with Fragaria x ananassa cv ...
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bioRxiv - Genetics 2021Quote: ... cloned into the pGEM-T Easy vector (Promega), and finally sequenced (SEQme ...
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bioRxiv - Physiology 2019Quote: ... or PGEM-T Easy Vector (Promega, Wisconsin, USA) using primers shown in Table 1 and cDNA from medaka brain and pituitary total RNA ...
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bioRxiv - Microbiology 2019Quote: ... Constructs were cloned into pGEM-T easy (Promega) or pJET1.2/blunt (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: Escherichia coli DH5α and pGEM-T easy (Promega) and pJET1.2/blunt (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... which was ligated into pGEM-T easy (Promega) and sequenced to ensure proper splicing ...
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bioRxiv - Plant Biology 2021Quote: ... A pGEM-T Easy vector (Promega, Madison, USA) cloned with a 450-bp fragment from SCYLV ORF3 previously amplified by RT-PCR was used to construct a serial dilution curve with six points and tenfold dilutions between points ...
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bioRxiv - Plant Biology 2021Quote: ... USA),cloned in pGEM-T easy vector (Promega, USA), and at least five individual clones were sequenced for each target.
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bioRxiv - Plant Biology 2020Quote: ... The amplificate was ligated into pGEM-T (Promega), from which the gene was excised with XhoI/EcoRI and ligated into pBAD/HisA (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were ligated to pGEM-T (Promega) or pBluescript SKII (− ...
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bioRxiv - Biochemistry 2022Quote: ... The product was cloned into pGEM-T (Promega) and the resulting rbs_csp3 fragment sub-cloned into pDR111 ...
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bioRxiv - Synthetic Biology 2022Quote: ... or pGEM®-T Easy (Promega, Madison, WI) followed by sequencing of plasmids isolated from 10-15 colonies ...
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bioRxiv - Neuroscience 2021Quote: ... or pGEM®-T Easy Vector System (Promega). Nucleotide sequences of primers were summarized in Table 1 (No ...
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bioRxiv - Microbiology 2021Quote: ... ligated into the pGEM T Easy vector (Promega) and transformed in DH10B chemo-competent cells ...
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bioRxiv - Cell Biology 2022Quote: ... t-ERK1/2 (Promega V114A, 1/1000 dilution), p-AKT (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2019Quote: ... cloned into the pGEM T-Easy vector (Promega; according to the manufacturer’s instruction ...
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bioRxiv - Epidemiology 2019Quote: ... and cloned into pGEM-T vector (Promega, USA). In total six 16S rDNA and two rpoB clones were sequenced and analysed using the BLASTN algorithm and finally aligned using Spiroplasma glossinidia data from NCBI as reference.
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bioRxiv - Molecular Biology 2020Quote: ... we ligated PCR amplicons to pGEM-T (Promega) vector system and confirmed that all picked clones encompassed TATA box deletion and guide6 reconstruction using Sanger sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... and cloned in pGEM-T easy vector (Promega); Four to six positive clones were sequenced (MWG ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cloned into pGEM-T Easy vectors (Promega, USA) and then transformed into competent Escherichia coli cells for amplification ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products were cloned into pGEM-T (Promega) and sequenced ...
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bioRxiv - Developmental Biology 2019Quote: ... and inserting it into pGEM-T Easy (Promega) by TA cloning ...
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bioRxiv - Cell Biology 2021Quote: ... Self-ligated empty pGEM-T vector (Promega, USA) was used to ensure equal amounts of transfected DNA ...
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bioRxiv - Cancer Biology 2021Quote: ... cloned into pGEM®-T Easy Vector (Promega), and then into the pSec-Tag2/hygroA vector (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... and TA cloned into pGEM-T Easy (Promega) then transformed into DH5α competent E ...