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Citations for Promega :
101 - 150 of 350 citations for Nucleoside diphosphate kinase A NDPKA Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Functional activity of rPfCDPK1 was monitored by utilizing ADP-Glo™ Kinase Assay (Promega Corporation), which is an ATP regeneration-based luciferase reaction system resulting from nascent ADP phosphorylation ...
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bioRxiv - Biochemistry 2024Quote: ... and the consumption of ATP was measured with ADP-Glo Kinase Assay kit (Promega Corporation) according to manufacturer protocol ...
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bioRxiv - Biochemistry 2020Quote: Functional activity of 6xHis-CDPK1 was monitored by utilizing ADP-Glo™ Kinase Assay (Promega Corporation), which is ATP regeneration based luciferase reaction system resulting from nascent ADP phosphorylation ...
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bioRxiv - Biochemistry 2021Quote: To investigate enzymatic activity of recombinant PfGSK3 a commercial luminescence-based kinase assay (KinaseGlo Plus, Promega) was used as previously described (93) ...
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bioRxiv - Cell Biology 2023Quote: ... Broad spectrum profiling on 192 kinases was performed using the K192 NanoBRET Target Engagement assay (Promega) with tracer K10 using the published protocol [24].
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bioRxiv - Biochemistry 2023Quote: The ATPase activity assay followed a previously established protocol using the ADP-Glo kinase assay (Promega)18 ...
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bioRxiv - Biochemistry 2021Quote: ... The 3xFlag-m-PKD1-2xMyc/His insert was subcloned into pCI vector (Promega). To produce CTF expression constructs of human (h ...
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bioRxiv - Cancer Biology 2021Quote: ... the PI3K lipid kinase reaction was performed in the presence of PIP2:3PS lipid substrate (Promega #V1792) and ATP ...
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bioRxiv - Cancer Biology 2020Quote: In vitro activity of PKM2 was performed using luminescent Kinase-Glo Plus reagent (Promega, cat. no. V3773,) and used according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Vmax and kcat values for given kinase substrates were determined using the ADP-Glo Kit system (Promega). In all cases reactions were carried out in 384 well plates at room temperature for 30 min with a total kinase concentration of 50 nM and 1 mM ultra-pure ATP ...
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bioRxiv - Biochemistry 2020Quote: ... GDP detection kit was from ProFoldin (MA, USA) and ADP-Glo™ Kinase Assay kit from Promega Corporation ...
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bioRxiv - Neuroscience 2019Quote: PC12 cells were transfected with the indicated Firefly Luciferase-IMPA1 constructs and thymidine-kinase – Renilla Luciferase (Promega) using Lipofectamine 2000 for 48hrs ...
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bioRxiv - Microbiology 2021Quote: The air-dried pellet containing the extracted MNase-digested RNA was treated with T4 polynucleotide kinase (Promega) to generate compatible ends for sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... CTPS activity of purified CTPS1 and CTPS2 proteins was then analyzed using ADP Glo Kinase assay (Promega). CTPS1 and CTPS2 were diluted in assay buffer (50 mM Trizma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The assay was carried out in 384-well plates using the ADP-Glo Kinase Assay kit (Promega), according to the published method [47] ...
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bioRxiv - Neuroscience 2021Quote: ... The ATP hydrolysis was detected upon addition of 50 μl/well luminescent Kinase Glo plus reagent (# V6711, Promega) using FLUOstar Omega microplate reader (BMG Labtech).
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bioRxiv - Molecular Biology 2019Quote: ... 25 mM creatine monophosphate [Sigma], 0.03 U/µL creatine kinase [Calbiochem], and 0.1 U/µL RNasin Plus RNase Inhibitor [Promega]) and 3.5 µL of each boiled supernatant in 10 µL lysis buffer at 25°C for 60 min ...
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bioRxiv - Microbiology 2020Quote: ... 50 pmoles of each probe was incubated with 50 pmoles of [γ32P]ATP and T4 polynucleotide kinase (Promega) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: The rate of ATP hydrolysis by cohesin was measured by using the ADP-Glo Kinase assay (Promega, TM313) as previously described (Panarotto et al. ...
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bioRxiv - Biochemistry 2023Quote: ... In brief: full-length kinases were obtained as plasmids cloned in frame with a terminal NanoLuc fusion (Promega) as specified in Table S3 ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of ADP Glo reagent then kinase detection reagent as specified by the manufacturer (Promega). Luminescence was measured in a 384-well plate using a TECAN Infinite M1000 microplate reader (Mannedorf ...
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bioRxiv - Biochemistry 2024Quote: The inhibitory potency (IC50) of harmine and AZ191 was determined with the ADP-Glo kinase activity assay (Promega). The synthetic peptide DYRKtide (RRRFRPASPLRGPPK ...
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bioRxiv - Biochemistry 2021Quote: The assay was performed as described previously.22,23 In brief: full-length kinases were obtained as plasmids cloned in frame with a terminal NanoLuc-fusion (Promega) as specified in Supplementary Table 6 ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...
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bioRxiv - Molecular Biology 2022Quote: ... Human Genomic DNA (G1521, Promega) was used as positive control and synthetic DNA was used to validate the LAMP assay performance ...
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bioRxiv - Biochemistry 2019Quote: ... The luminescence signal was developed for 20 min after adding 40 µl kinase detection reagent (kit) and measured with a GloMaxMulti plate reader (Promega). The average of three experiments performed in duplicate and normalized to PI3KC3-C1 activity were determined ...
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bioRxiv - Biophysics 2021Quote: ... Samples were incubated at 25°C for 30 minutes with gentle shaking before addition of 50 µL Kinase GloTM Max reagent (Promega) and further incubation at 25°C for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: Identification of the β5-integrin carboxyl domain as Src substrate was determined using the ADP-Glo Kinase Assay (Promega, V6930) following protocols recommended by the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Bone Morphogenetic Protein 4 (BMP4, 10 ng/ml, Bio-Techne) plus phosphoinositide 3-kinase inhibitor Ly294002 (10 µM, Promega). After 42 h incubation at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... The primer and DNA marker were end-labeled with 10 μCi of [γ-32P]ATP by T4 polynucleotide kinase (Promega) and then used for reverse transcription with avian myeloblastosis virus (AMV ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Kinase activity of phosphorylated CDK2 with cyclin A and varying amounts of inhibitor (dinaciclib or 5) were measured using the ADP-Glo Kinase Assay (Promega) in a luminescence plate reader (Tecan Infinite M1000 PRO) ...
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bioRxiv - Microbiology 2022Quote: Purified recombinant His6-SBP-tagged HipBLp, HipSLp, and HipTLp (WT, D197Q, D219Q) were tested for ATP hydrolytic activity using the ADP-Glo kinase assay (Promega) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was incubated at 25°C for 30 minutes and the production of ADP was measured using the ADP-Glo kinase assay kit (Promega). Vmax was calculated after fitting the kinase activity data to the Michaelis–Menten equation ...
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bioRxiv - Biochemistry 2023Quote: The assay was performed as described previously.32 In brief: full-length kinases were obtained as plasmids cloned in frame with a terminal NanoLuc-fusion (Promega) as specified in Supplementary Table S3 ...
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bioRxiv - Biochemistry 2023Quote: The assay was performed as previously described.61 In brief: full-length kinases were obtained as plasmids cloned in frame with a terminal NanoLuc-fusion (Promega) as specified in Table S4 ...
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bioRxiv - Biochemistry 2024Quote: The assay was performed as described previously.38 In brief: full-length kinases were obtained as plasmids cloned in frame with a terminal NanoLuc-fusion (Promega) as specified in Supplementary Table S5 ...
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bioRxiv - Microbiology 2020Quote: ... 500 ng human genomic DNA (Promega) or 5 μl of untreated or RNase-treated nasal swab nucleic acid isolates ...
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bioRxiv - Cancer Biology 2020Quote: ... Pooled human male genomic DNA (Promega) was randomly sheared (500-800bp ...
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bioRxiv - Systems Biology 2021Quote: ... a human cDNA pool (Promega Corporation), or obtained as synthesized double-stranded DNA fragments (gBlocks ...
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bioRxiv - Genomics 2021Quote: ... 50ng human genomic DNA (Promega, G304A), 2µM assembled T7-Tn5 transposase or Tn5 transposase was incubated at 55 °C for 7minutes ...
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bioRxiv - Immunology 2023Quote: ... Lumit Human IL-1β Immunoassay (Promega) was performed according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... human Female genomic DNA (Promega #G1521), as a reference ...
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bioRxiv - Cell Biology 2019Quote: ... University of Wisconsin), and 1ng HSV-thymidine kinase promoter driven Renilla luciferase (pRL-TK-Renilla, referred to as Renilla) (Promega, E2241) per well using Lipofectamine 2000 (Life technologies ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... miR159 and U6 RNA (see Table S3) were 5’ end-labelled with [γ-32P]ATP using T4 polynucleotide kinase (PNK) (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... The radioactively labeled probe was first prepared with γ[32 P]ATP ATP and DNA oligonucleotides listed in Supplementary Table S5 with a T4 polynucleotide kinase kit (Promega), purified on MicroSpin G-25 (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: After extraction of proteins (see above) without proteinase and phosphatase inhibitors the Src activity was evaluated with ProFluor® Src-Family Kinase Assay (Promega).
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bioRxiv - Microbiology 2023Quote: ATPase assays for the SAR studies were performed per manufacturer’s recommendations using the ADP-Glo Kinase Assay (Promega Corp, Madison WI). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The experiments to evaluate the inhibitory activity levels of compounds were carried out according to the manufacturer’s instructions on the SYK Kinase Enzyme System (Promega, Wisconsin, USA) and ADP-GloTM Kinase Assay (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant was collected and purification was done via His-tag using HisLink Protein Purification Resin (V8823, Promega) according to manufacturer’s instructions ...