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Citations for Promega :
1 - 50 of 1442 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... All buffers and gradients used for nuclear extraction contained RNase inhibitor (Promega) at 60 U/mL.
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... Nuclear extracts of equal protein amounts were first subjected to proteolysis with trypsin (V5113, Promega) at 37°C for 16 hours ...
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bioRxiv - Genomics 2023Quote: ... Protein was precipitated by the addition of 200 μl Protein Precipitation Solution (Promega Wizard HMW DNA Extraction Kit A2920). Using a wide bore tip the samples were mixed by drawing up contents from the bottom of the tube and then expelled on the side of the tube 5 times ...
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bioRxiv - Molecular Biology 2023Quote: DNA extraction was performed using the Wizard genomic DNA extraction kit (Promega). Genomic DNA (1μg ...
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bioRxiv - Neuroscience 2023Quote: ... before DNA extraction using the Wizard SV genomic DNA extraction kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Ca2+-dependent nuclear factor of activated T-cells (NFAT) (pGL4.30; Promega), or the MAPK signaling pathway (pGL4.33 ...
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bioRxiv - Plant Biology 2023Quote: ... leaf samples of equal size were collected for protein extractions using the Dual-Luciferase Reporter Assay System kit (Promega, Fitchburg, USA). Glomax 96 microplate luminometer (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Fragmented nuclear DNA was detected by DeadEnd Fluorometric TUNEL assay (Promega, Madison, WI) that coupled fluorescein-12-dUTP to the 3’-OH ends of the DNA ...
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bioRxiv - Physiology 2019Quote: ... and then harvested for RNA extraction with the RNA extraction kit (Promega, Madison, WI, United States), followed with reverse transcription using ReverTra Ace qPCR RT Master Mix with gDNA Remover kit (TOYOBO) ...
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bioRxiv - Microbiology 2019Quote: ... and DNA extraction and purification were performed in a Maxwell® 16 DNA extraction system (Promega) using the LEV DNA kit (AS1290 ...
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bioRxiv - Microbiology 2022Quote: ... After extraction a Turbo DNase (Promega) digest was performed as per the manufacturer’s instruction and total RNA yield was quantified using a Qubit RNA broad spectrum assay kit as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... RNA of roots and nodule primordia was extracted using a TRIzol (Invitrogen)-adapted ReliaPrep RNA extraction kit (Promega), as previously described (53) ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted using a TRIzol (Invitrogen)-adapted ReliaPrep RNA extraction kit (Promega) [39] ...
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bioRxiv - Microbiology 2021Quote: Forty-nine confirmed Bp isolates were subjected to DNA extraction using the Wizard Genomic Extraction Kit (Promega, USA). For whole genome sequencing (WGS) ...
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bioRxiv - Plant Biology 2023Quote: ... multiflorum samples using a commercial DNA extraction kit (Wizard HMW DNA Extraction Kit, Promega Corporation, Madison, WI, USA).
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bioRxiv - Cancer Biology 2021Quote: ... and Maxwell nucleic-acid extraction instrument (Promega). Nanodrop quantified RNA was checked by Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies) ...
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bioRxiv - Plant Biology 2020Quote: ... the SV Total RNA Extraction Kit (Promega) was used following the product specifications ...
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bioRxiv - Microbiology 2021Quote: ... or the Wizard gDNA extraction kit (Promega). The Quick-DNA miniprep kit (Zymo ...
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bioRxiv - Molecular Biology 2022Quote: ... Using an RNA extraction kit (Promega, China), total RNA was extracted from 4- ...
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bioRxiv - Bioengineering 2023Quote: ... RNeasy mini prep RNA extraction kit (Promega); phosphate buffered saline (PBS) ...
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bioRxiv - Immunology 2024Quote: ... and total RNA extraction was performed (Promega) according to manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized from nuclear and cytoplasmic RNA using Improm II RT (Promega, Cat. #A3800), and cDNA levels were measured by qPCR using the Brilliant III Ultra-Fast qPCR Kit (Agilent Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... Leaf tissue was collected from plants for high-molecular weight DNA extraction (Wizard HMW DNA Extraction, Promega, Madison, WI). We used flow cytometry to estimate genome size relative to Conyza canadensis and Solanum lycopersicum ...
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bioRxiv - Plant Biology 2024Quote: ... RNA extraction was performed using an RSC 48 RNA extraction robot (Maxwell) and the Maxwell RSC Plant RNA kit (Promega). Although the RNA yield for some of the 64 samples was low ...
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bioRxiv - Biochemistry 2020Quote: ... Two control reactions were performed: one containing 8 units of HeLa nuclear extract provided by Promega as a positive control and the other containing no nuclear protein as a negative control ...
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bioRxiv - Microbiology 2020Quote: Wizard Promega DNA extraction kits (Promega, Madison, WI) was used to extract DNA from overnight broth culture ...
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bioRxiv - Genetics 2021Quote: ... and Tissue and Hair Extraction Kit (#DC6740, Promega) for Maxwell® systems ...
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bioRxiv - Microbiology 2024Quote: ... on extracted genomic DNA (Promega Wizard DNA Extraction) testing 3 amplifications using primers P4 and P5 ...
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bioRxiv - Microbiology 2022Quote: ... A total of 56 Fusobacterium isolates were recovered and subjected to DNA extraction using the Wizard genomic extraction kit (Promega, USA). For each isolate ...
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bioRxiv - Microbiology 2023Quote: ... of virus pool stock was used for viral RNA extraction by GeneAid viral RNA extraction kit (GeneAid) and converted to cDNA using ImProm-II™ reverse transcription system (Promega) according to manufacturers’ protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclear pellets obtained after centrifugation at 1,500g for 3 min were lysed in Reporter lysis buffer (Promega), passed through a 21Gneedle and incubated on ice for 10 min before centrifugation at 17,000g ...
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bioRxiv - Biochemistry 2020Quote: Transcription assays were set up using the HeLa Scribe Nuclear Extract in vitro Transcription system (Promega, catalog # E3110) following the manufacture’s protocol with modifications ...
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bioRxiv - Immunology 2020Quote: ... 200 µl/sample) and split into fractions with or without MNase (Promega, 50U/100 µl of nuclear lysate) and incubated for 1 hour at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... followed by standard mini prep extraction per manufacturer’s instructions (Promega). The pooled plasmid library was suspended in 50 μL of sterile water ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... implemented on a Maxwell® 16 automated extraction system (Promega). Genomic DNA quantity was assessed using a Qubit 2.0 fluorometer with the dsDNA BR Assay (Life Technologies ...
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bioRxiv - Zoology 2020Quote: DNA was extracted using the Wizard DNA extraction kit (Promega), the Qiagen Blood & Tissue kit ...
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bioRxiv - Molecular Biology 2023Quote: ... or using the Wizard HMW DNA Extraction Kit (Promega; A2920) following the manufacturer’s protocol with modifications that RNase and Proteinase treatments were each extended to one hour ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-dependent interactions were assessed by treating nuclear extracts with 1 U/μl RNasin® Ribonuclease Inhibitors (Promega, # N2615) as control or 1 mg/ml of RNase A (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was performed using the Maxwell Cell tissue kit (Promega). The primers ...
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bioRxiv - Microbiology 2019Quote: ... gDNA was isolated using the Wizard Genomic DNA extraction kit (Promega). gDNA was then fragmented to 400-600 bp by sonication (Covaris E220 ...
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bioRxiv - Neuroscience 2019Quote: ... All buffers and gradient solutions for nuclei extraction contained RNAsin (Promega) and 0.04% BSA.
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Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survivalbioRxiv - Cancer Biology 2022Quote: ... RNA-extraction was carried out according to the manufacturer’s protocol (Promega ReliaPrepTM RNA Tissue Miniprep System ...
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bioRxiv - Neuroscience 2020Quote: ... RNA extraction was performed using ReliaPrep Tissue RNA Miniprep system (Promega). RNA quantity and integrity was analyzed using Nanodrop (Thermo Fisher ...
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bioRxiv - Molecular Biology 2019Quote: ... To extract RNA the SV Total RNA extraction KIT (Promega®) was used following the product specifications ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-extraction was carried out according to the manufacturer’s protocol (Promega ReliaPrepTM RNA Tissue Miniprep System ...
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bioRxiv - Genomics 2020Quote: ... Typhi using the Wizard genomic DNA extraction Kit (Promega, Wisconsin, USA) and subjected to WGS using the Illumina MiSeq platform (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... gDNA was isolated using the Wizard Genomic DNA extraction kit (Promega). gDNA was fragmented to 400-600 bp using a Covaris E200 sonicator and end-repaired using the NEB Quick Blunting Kit ...
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bioRxiv - Microbiology 2023Quote: ... All RNA extractions were performed on a Maxwell 16 instrument (Promega) with the Maxwell RSC Viral Total Nucleic Acid Purification Kit and eluted in 50uL of nuclease-free water ...
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bioRxiv - Neuroscience 2023Quote: ... RNA extraction was performed using ReliaPrep RNA Cell Miniprep System (Promega). All biological replicates were performed on the same day ...
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bioRxiv - Genetics 2023Quote: ... then submitted to RNA extraction using the RNeasy Mini Kit (Promega). cDNA conversion was performed combining the SuperScript II Reverse Transcriptase (Invitrogen ...