Labshake search
Citations for Promega :
201 - 250 of 1753 citations for Nipah Virus Glycoprotein F Recombinant Protein Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... selections were performed using biotinylated c-CDCP1-Fc captured on SA-coated magnetic beads (Promega). Prior to each selection ...
-
bioRxiv - Bioengineering 2023Quote: ... selections were performed using biotinylated Fc fusion antigen captured on SA-coated magnetic beads (Promega). Prior to each selection ...
-
bioRxiv - Cell Biology 2022Quote: ... Nuclei were resuspended in reaction buffer [RNase inhibitor (0.2U/μL Recombinant RNAsin (Promega, PAN2515), 1% Fatty-acid-free BSA in PBS (Proliant ...
-
bioRxiv - Microbiology 2024Quote: Recombinant FIKK kinase domains activity was measured using the ADP-Glo kinase assay (Promega), which quantifies the amount of ADP produced during the kinase reaction ...
-
bioRxiv - Developmental Biology 2020Quote: ... The RNA (50-250 ng) was reverse-transcribed with the Moloney Murine Leukemia Virus reverse transcriptase (M-MLVRT) (Promega, M368B) and amplified using the iTaqTM Universal SYBR® Green Supermix (Bio-Rad ...
-
bioRxiv - Plant Biology 2020Quote: ... Reverse transcription was achieved with 4 µg of RNAs in the presence of Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase (RNase H minus, Point Mutant, Promega) after annealing with an anchored oligo(dT)18 primer as described (Wirth et al. ...
-
bioRxiv - Molecular Biology 2019Quote: ... WT and m6A-mut expressing cells in 24-well plates were infected with the indicated virus (MOI 1) and dual luciferase assay (Promega) was performed at 48 hours post-infection according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... of 100 ng of RNA was performed as previously described [49] using Moloney Murine Leukemia virus (M-MLV) reverse transcriptase (Promega), with an extension temperature of 42°C for 60 min and random hexamer (IDT) ...
-
bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
-
bioRxiv - Neuroscience 2020Quote: ... oligo-dT primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus Reverse Transcriptase (Promega). RT-qPCR was performed on the ViiA 7 Real-Time PCR System using GoTaq qPCR master mix (Promega ...
-
bioRxiv - Molecular Biology 2020Quote: ... Resulting ribosomal complexes were analyzed by toe-printing as described (Pisarev et al., 2007) using avian myeloblastosis virus reverse transcriptase (AMV RT) (Promega) and [32P]-labeled oligonucleotide primers complementary to HalV nt ...
-
bioRxiv - Cell Biology 2022Quote: ... synthesis was done by using 1 μg RNA as template and the Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase (Promega), in 20 μl reaction volume according to the instructions manual ...
-
bioRxiv - Genetics 2021Quote: ... Around 1 μg total RNA was used to synthesize cDNA by using oligo-dT18 primer and Moloney murine leukemia virus reverse transcriptase (M1701, Promega). The RT-qPCR was performed using iTaq Universal SYBR Green Supermix (172-5125 ...
-
bioRxiv - Plant Biology 2021Quote: ... reverse transcribed with oligo(dT)15 in a 20-μL reaction mixture using the Moloney murine leukemia virus reverse transcriptase (Promega) or the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... 0.5 ml of culture supernatants were collected every 6 hours and nanoluciferase activities associated with released virus in the supernatants were measured using Nano-Glo luciferase assay system (Promega).
-
bioRxiv - Microbiology 2022Quote: ... Cells were incubated for three days before assessing viability and virus-induced cell death by CellTiter Glo according to manufacturer protocol (Promega). Luminescence was quantified using a plate reader (Cytation 5 ...
-
bioRxiv - Microbiology 2022Quote: ... The RNA was used for first-strand cDNA synthesis using Moloney murine leukemia virus (MMLV) reverse transcriptase enzyme (Promega, USA) and random primers (Biodynamics ...
-
bioRxiv - Physiology 2022Quote: ... and 0.5 μg of reference plasmid pRL-TK carrying the Renilla luciferase gene under the control of the simian virus 40 enhancer and promoter (Promega). Lipofectamine 3000 reagent (Invitrogen ...
-
bioRxiv - Microbiology 2022Quote: ... We estimated the number of infectious virus after two days by quantifying the luminescence in each well using Bright Glo Luciferase Assay System (Promega). Differences in relative luciferase units (RLUs ...
-
bioRxiv - Microbiology 2022Quote: ... Complementary DNA was synthetized from 500 ng total RNA by using random hexamers and moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) Point Mutant Synthesis System (Promega). Quantification was performed by using the LightCycler 480 Universal Probe Library System (Roche) ...
-
bioRxiv - Microbiology 2023Quote: Cells infected with a luciferase-encoding virus were lysed 2 days after infection with a Bright-Glo Luciferase Assay System (E2620, Promega) and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega).
-
bioRxiv - Cancer Biology 2024Quote: ... 1ug of each RNA sample was reverse-transcribed using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV-RT, Promega, #M1701) and random hexamer primers (Thermo Scientific ...
-
bioRxiv - Microbiology 2020Quote: Recombinant TgGat1 (0.625-2.5 µM) was incubated with a given UDP-sugar (50 µM) (Promega) in 50 mM HEPES-NaOH (pH 7.4) ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... QuantiLum Recombinant Luciferase and the BrightGlo Luciferase Assay System were acquired from Promega (Madison, WI). The HIV-1 inhibitor temsavir was acquired from ViiV Healthcare (Brentford ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant TgPDEs or immunoprecipitated TgPDEs were assayed using the PDE-Glo Phosphodiesterase Assay kit (Promega) according to the manufacturer’s instructions with a few modifications ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
-
bioRxiv - Immunology 2020Quote: ... Mice were tested individually in duplicates by stimulation with recombinant luciferase (13μg/ml, Promega, #E1701), SARS-CoV-2 spike protein (10μg/ml) ...
-
bioRxiv - Plant Biology 2023Quote: ... in the presence of 20 units of RNasin (Recombinant Ribonuclease Inhibitor, Cat. No. C2511; Promega) and dNTP mix at a final concentration of 0.5 mM of each dNTP (Cat ...
-
bioRxiv - Cell Biology 2023Quote: ... all the steps were done in the presence of RNase inhibitor (recombinant RNasein, Promega, N2511) diluted 1:4,000 for washing steps or 1:400 for incubation while staining and for buffers in the tubes containing the sorted cells followed by snap freeze and storage at –80°C.
-
bioRxiv - Biochemistry 2023Quote: ... 10 μg of the isolated recombinant bacmid and 5 μL Fugene HD transfection reagent (Promega) were diluted in 150 μL Sf-900™ III SFM ...
-
bioRxiv - Microbiology 2022Quote: ... Relative F-Luc activity compared to R-Luc was quantified using a dual luciferase assay (Promega, E1960).
-
bioRxiv - Physiology 2020Quote: ... oligo-dT primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus reverse transcriptase (Promega, USA). qRT-PCR was performed using a ViiA Real-Time PCR System (Applied Biosystems ...
-
bioRxiv - Microbiology 2019Quote: ... the cell viability of each infected virus variants was measured using CellTiter 96 Aqueous One solution Cell Proliferation Assay (Promega, USA). The relative cell viability was calculated by normalizing the absorbance value of treated virus samples against untreated virus samples ...
-
bioRxiv - Microbiology 2019Quote: ... 4 × Promega Wizard® Minicolumns per replicate with a ratio of 0.5:1 virus concentrate to Wizard® PCR Preps DNA Purification Resin (Promega: A7170). The resultant extract was found to be inhibitory to all enzymatic reactions ...
-
bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
-
bioRxiv - Microbiology 2022Quote: ... We performed duplicate serial dilutions using supernatants collected from the virus rescues and measured luciferase expression at each dilution using Bright-Glo Luciferase Assay System (Promega, E2610). Virus titers were calculated as relative light units (RLU ...
-
bioRxiv - Molecular Biology 2023Quote: ... Produced pseudoviruses were titrated on HEK-293T-ACE2 by performing duplicate serial dilutions and virus titers were measured 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610).
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated at 37 °C for two days and the nLuc activity from the virus replication was measured by using Nano-Glo® Luciferase Assay System (Promega) following the manufacturer’s protocol using a plate reader (HT4 Biotek) ...
-
bioRxiv - Immunology 2024Quote: ... The virus neutralization titers were determined by measuring the NLuc activity using the Nano-Glo Luciferase Assay System (Promega, Madison, WI) following the manufacturer’s conditions ...
-
bioRxiv - Biochemistry 2022Quote: ... The scFv gene was then ligated into pCSE2.6-hIgG1-Fc-XP vector (43) using T4 Ligase (Promega) and transformed into E ...
-
bioRxiv - Biochemistry 2021Quote: To investigate enzymatic activity of recombinant PfGSK3 a commercial luminescence-based kinase assay (KinaseGlo Plus, Promega) was used as previously described (93) ...
-
bioRxiv - Molecular Biology 2021Quote: ... These recombinant plasmids were subsequently used to generate digoxigenin-labelled riboprobes using the Riboprobe System (Promega) with SP6 or T3 RNA polymerases and digoxigenin-labelled Uracil triphosphate (Roche ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant EbfC was expressed in Escherichia coli Rosetta II cells and purified using MagneHis Particles (Promega) as previously described (24 ...
-
bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
-
bioRxiv - Cell Biology 2023Quote: ... Dystrophin gene editing was verified by PCR for mouse dystrophin using primers Dmd_i22-i23.F/ Dmd_i22-i23.Rand GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...
-
bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed with primers Dmd_e22-e24.F / Dmd_e22-e24.R using GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were then digested with LysC at 1ug/100ug protein (Promega) for 3 h at room temperature (RT ...
-
bioRxiv - Plant Biology 2020Quote: ... Fusion proteins were purified using MagnetHis™ protein purification system (Promega) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2024Quote: ... Proteins were precipitated through the addition of Protein Precipitation Solution (Promega) followed by vigorous vortexing ...
-
bioRxiv - Plant Biology 2020Quote: ... corniculatus plants was synthesized from 3 μg of total RNA using a Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) (Promega, WI, USA) and 100 pmol of random hexamers (Pharmacia Biotech) ...