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Citations for Promega :
1 - 50 of 1364 citations for Myelin Basic Protein Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... pGL2-Basic (Promega) Vcam-1 promoter construct (VC1889 ...
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bioRxiv - Cell Biology 2023Quote: pGL2-Basic (Promega, E1641) or pGLCD36 (Shore ...
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bioRxiv - Microbiology 2024Quote: ... pGL3-basic vector (Promega) via SmaI and HindIII sites to generate pBAH8 ...
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bioRxiv - Cancer Biology 2020Quote: ... pGL3-basic (Promega, Madison, WI), was used ...
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bioRxiv - Cancer Biology 2021Quote: ... the pGL3-Basic vector (Promega) was used ...
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bioRxiv - Genomics 2023Quote: ... and pGL3-basic (Promega, E1741).
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bioRxiv - Cell Biology 2024Quote: ... CytoLuc-pGL3 basic (Promega #E1751), pLVX-NEO-CMV-2mt-GCaMP6s and SNAP25-Aequorin were kindly provided by Dr ...
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bioRxiv - Biophysics 2022Quote: ... Cleaved MSP protein was subsequently biotinylated using EZ-Link Maleimide-PEG11-Biotin (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The purified Kif5B protein was incubated with 10 µM HaloTag PEG-Biotin ligand (Promega) for 30 min on ice to produce a final construct of Kif5B homodimer with two C-terminal biotin tags ...
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bioRxiv - Molecular Biology 2019Quote: ... subcloned into pGL3-Basic-Luciferase vector (Promega) containing a firefly luciferase reporter gene and verified by DNA sequencing ...
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bioRxiv - Developmental Biology 2022Quote: ... into pGL3-Basic (Promega, Madison WI, USA) vector linearized with XhoI/HindIII (NEB ...
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bioRxiv - Immunology 2021Quote: The pGL3-Basic Luciferase Reporter vector (Promega), pcDNA3.1 plasmid ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples eluted with biotin were digested in-solution with 1 ug Trypsin/LysC (Promega) overnight at 37°C ...
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bioRxiv - Biophysics 2023Quote: Labeling reactions: Proteins with HaloTag were labeled with JF646-HaloTag ligand (Promega # GA1120) by incubating the proteins with a tenfold excess of dye on ice for 0.5-1 h in the presence of 1 mM ATP ...
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bioRxiv - Molecular Biology 2019Quote: ... we used the pGL3-Basic vector (Promega, USA) with minor modification ...
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bioRxiv - Cell Biology 2022Quote: ... and ligated into the pGL3-basic vector (Promega). Then ...
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bioRxiv - Physiology 2020Quote: ... and was cloned in pGL3 basic vector (Promega). Oxygen stable HIF-1α ...
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bioRxiv - Cell Biology 2020Quote: ... were all constructed into pGL3-basic vector (Promega). All plasmid constructs were transfected into COS-7 cells or cardiomyocytes with FuGENE® HD ...
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bioRxiv - Genomics 2022Quote: ... Both the pGL3-Basic Luciferase vector (Promega, E1751) and the CDC20 promoter amplicon were digested using SacI-HF (NEB ...
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bioRxiv - Evolutionary Biology 2022Quote: ... These 89 consensus sequences were then synthesized by Genscript and cloned into the pGL3 Basic vector (Promega) with an added minimal promoter (pGL3Basic[minP] ...
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bioRxiv - Cancer Biology 2023Quote: A luciferase reporter system (pGL3 basic vector, Promega) was used to validate the direct interaction of XBP1s and the predicted promoter regions of PD-1 as well as the individual XBP1s binding sites identified within PD-1 promoter regions ...
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bioRxiv - Cancer Biology 2022Quote: ... Both the pGL3-Basic Luciferase vector (Promega, E1751) and the CDC20 promoter amplicon were digested using SacI-HF (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... [35S]-labeled Zc3h10 protein was produced by using TNT coupled transcription/translation kit (Promega). 20 ug of GST fusion proteins were incubated for 2 hrs at 4°C with in vitro translated Zc3h10 and glutathione sepharose beads ...
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bioRxiv - Biochemistry 2020Quote: The two Munc13-Halo proteins were labeled by incubating the protein with Alexa 488 conjugated with HaloTag® from Promega, as described before (2) ...
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bioRxiv - Molecular Biology 2019Quote: ... Luciferase reporter gene construction The pGL3 Basic vector (Promega) was used as a backbone ...
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bioRxiv - Microbiology 2020Quote: ... and ligation into KpnI/XhoI-digested pGL3-Basic (Promega).
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bioRxiv - Microbiology 2020Quote: ... and ligation into KpnI/XhoI-digested pGL3-Basic (Promega). Mutations to the NF-κB and IRF binding sites in pLTR(Sp1)-luciferase were generated using the QuickChange IIXL site-directed mutagenesis kit (Stratagene) ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... into the MluI/BglII sites of pGL2-Basic (Promega). The following reagent was obtained through the NIH AIDS Reagent Program ...
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bioRxiv - Systems Biology 2023Quote: ... + P/S + 8 ng/mL rhFGF-basic (Promega #G5071) + 2 ug/mL doxycycline ...
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bioRxiv - Biochemistry 2023Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (T7, Promega). Translation products were partially purified by ammonium sulfate precipitation [87] ...
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bioRxiv - Molecular Biology 2023Quote: ... we labeled HALO-tagged49 proteins with HALO-Alexa488 or HALO-TMR dyes (Promega, G1001, G8251) by incubating the concentrated protein with dye for 1 hour on ice ...
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bioRxiv - Cell Biology 2021Quote: ... the bead-bound protein was incubated with either 6.7 μM HaloTag-TMR or HaloTag-PEG-biotin ligand (Promega) for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: The ABCB1 promoter was cloned in a pGL3-basic (Promega) vector (pGL3-Basic Vector GenBank® Accession Number U47295) ...
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bioRxiv - Genomics 2022Quote: ... 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 ng pGL3-Basic luciferase reporter plasmid (Promega, Cat. #E1751) with target sequence inserts were cotransfected into HEK293T and MCF7 cell lines with 200 ng of Control/YAP1-S127A construct and 10 ng of Renilla luciferase pRL-TK plasmid (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... The Drd4 promoter was subcloned into pGL3 Basic (Promega, E1751) vector using XhoI and HindIII enzyme sites ...
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bioRxiv - Biochemistry 2022Quote: ... [35S]-labeled proteins were synthesized in vitro via a coupled transcription/translation rabbit reticulocyte kit (Promega) incubated at 30°C for 90 min with [35S]Met/Cys metabolic labeling reagent (MP Biomedical ...
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bioRxiv - Biochemistry 2021Quote: Mitochondrial 35S-labeled precursor proteins were synthesized using the TNT SP6-coupled transcription/translation kit (Promega) and plasmid-vectors pSP64 containing the genes of interest ...
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bioRxiv - Molecular Biology 2022Quote: ... were incubated with 35S labeled proteins made by using TNT Quick Coupled transcription/Translation System (Promega) with 35S labeled methionine ...
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bioRxiv - Cell Biology 2023Quote: ... 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega). Translation reaction products from 0.25 μg of plasmid DNA were incubated with GST-labeled proteins on glutathione-Sepharose beads in NETN-E buffer (50 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... Transcend Biotin-Lysyl-tRNA (Promega) was incorporated in reactions so that reaction products could be identified using western blotting as above ...
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bioRxiv - Biochemistry 2023Quote: HaloTag-PEG-biotin ligand (Promega, cat ...
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bioRxiv - Cell Biology 2021Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (SP6 or T7, Promega). Translation products were partially purified by ammonium sulfate precipitation (Bardwell & Shah ...
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bioRxiv - Cell Biology 2020Quote: All Luciferase vectors were derived from PGL3-basic vector (Promega, E1751) by Seamless Cloning ...
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bioRxiv - Immunology 2021Quote: ... The Spic promoter was cloned into pGL3-Basic (Promega, Madison, WI) using HindIII cut sites ...
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bioRxiv - Developmental Biology 2021Quote: ... and Antp promoters were subcloned into the pGL3-basic vector (Promega). The ORF of red fluorescent protein gene (RFP)-fused Antp was inserted into a pIZ/V5-His vector (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... The corresponding cDNA was subcloned into pGL3 basic (Promega, no. E1751) upstream to sequence encoding redfirefly luciferase ...
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bioRxiv - Systems Biology 2020Quote: ... + P/S + 0.5μg/mL Puromycin + 16ng/mL rhFGF-basic (Promega G5071).
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bioRxiv - Immunology 2020Quote: pGL4.10-Basic and pRL-TK luciferase reporter vectors (Promega, Madison, WI) were used for the promoter reporter assays ...
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bioRxiv - Molecular Biology 2020Quote: ... These promoter sequences were designed and cloned into pGL2-Basic (Promega), a luciferase reporter plasmid ...