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Citations for Promega :
151 - 200 of 209 citations for Mumps Virus Nucleoprotein Strain Jeryl Lynn since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... strain ADP were extracted using Wizard™ Genomic DNA Purification Kits following the standard protocol provided by Promega.
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bioRxiv - Physiology 2020Quote: ... oligo-dT primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus reverse transcriptase (Promega, USA). qRT-PCR was performed using a ViiA Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... the cell viability of each infected virus variants was measured using CellTiter 96 Aqueous One solution Cell Proliferation Assay (Promega, USA). The relative cell viability was calculated by normalizing the absorbance value of treated virus samples against untreated virus samples ...
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bioRxiv - Microbiology 2019Quote: ... 4 × Promega Wizard® Minicolumns per replicate with a ratio of 0.5:1 virus concentrate to Wizard® PCR Preps DNA Purification Resin (Promega: A7170). The resultant extract was found to be inhibitory to all enzymatic reactions ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Microbiology 2022Quote: ... We performed duplicate serial dilutions using supernatants collected from the virus rescues and measured luciferase expression at each dilution using Bright-Glo Luciferase Assay System (Promega, E2610). Virus titers were calculated as relative light units (RLU ...
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bioRxiv - Molecular Biology 2023Quote: ... Produced pseudoviruses were titrated on HEK-293T-ACE2 by performing duplicate serial dilutions and virus titers were measured 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated at 37 °C for two days and the nLuc activity from the virus replication was measured by using Nano-Glo® Luciferase Assay System (Promega) following the manufacturer’s protocol using a plate reader (HT4 Biotek) ...
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bioRxiv - Immunology 2024Quote: ... The virus neutralization titers were determined by measuring the NLuc activity using the Nano-Glo Luciferase Assay System (Promega, Madison, WI) following the manufacturer’s conditions ...
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bioRxiv - Plant Biology 2020Quote: ... corniculatus plants was synthesized from 3 μg of total RNA using a Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) (Promega, WI, USA) and 100 pmol of random hexamers (Pharmacia Biotech) ...
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bioRxiv - Molecular Biology 2021Quote: cDNA synthesis for gene expression analyses was performed using 500 ng RNA using Moloney murine leukemia virus (M-MLV) reverse transcriptase and random hexamer oligonucleotide (both from Promega, Southampton, UK) using 500 ng RNA in a 25 µl reaction ...
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bioRxiv - Biochemistry 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (MMLV-RT) enzyme and recombinant RNasin® ribonuclease inhibitor were purchased from Promega (Madison, WI, USA). Evagreen 2X qPCR MasterMix was purchased from Applied Biological Materials Inc ...
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bioRxiv - Cell Biology 2021Quote: ... and 1-3μl aliquots were taken for reverse transcription (RT) using the Moloney murine leukemia virus RT RNase H (−) (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The resulting materials were used as template to generate cDNA using 100 U Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI), 0.45 μM N6 random hexamer primers (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from this concentrated virus sample using a modified protocol with the Wizard Genomic DNA Purification Kit A1120 (Promega, Madison, WI). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... virus replication was quantified by measuring Renilla luciferase activity (relative light units [RLU]) using Bright-Glo™ Luciferase Assay System (Promega, USA) 48 hours post-reinfection ...
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bioRxiv - Microbiology 2019Quote: ... Virus infectivity was quantified by measuring Renilla luciferase activity (relative light units [RLU]) using Bright-Glo™ Luciferase Assay System (Promega, USA) on the Tecan microplate reader (Tecan Infinite® 200 Pro ...
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bioRxiv - Cell Biology 2020Quote: ... Total 500 ng RNA was used to synthesize cDNA by using Moloney-murine leukemia virus (M-MLV) reverse transcriptase (Promega, Madison, WI). Diluted cDNA was used in each quantitative reverse transcription-PCR (qRT-PCR) ...
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bioRxiv - Plant Biology 2022Quote: ... cDNAs were synthesized from 1.5 μg of total RNA with oligo(dT) primers using Moloney Murine Leukemia Virus Reverse Transcriptase MMLV-RT according to the manufacturer’s instructions (Promega, Madison, WI, USA). RT-qPCR was carried out in a Chromo4 system (Bio-Rad ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37°C under 5% CO2 for 144 hour and virus cytopathic effect was measured using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Cat. # G9241) following the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... the amount of input virus was normalized to the HiBiT value measured by Nano Glo HiBiT lytic detection system (Promega, Cat# N3040)] ...
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bioRxiv - Microbiology 2023Quote: ... Samples where a 1 log decrease in RLU compared to the no-serum virus-only control was observed were then selected for cytotoxicity assay using Cell Titre Glo kit (Promega, Southampton, UK) and light microscopy to verify viability of cells prior to undertaking PVNA (data not shown) ...
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bioRxiv - Microbiology 2024Quote: ... followed by reverse transcription to complementary DNA (cDNA) using 30 enzyme units(U) of the Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) (Promega, WI, USA). The extension products were then analysed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: Strains were grown to log-phase and luciferase assays were conducted using Nanoglo Luciferase Assay System (Promega, Fitchburg, WI). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA of all the strains was isolated using the Wizard genomic DNA purification kit (Promega, Madison, WI, USA), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... selected Δku80 and Δku80_Δpks1 strains were plated on Sabouraud and YPD agar paltes supplemented with 0.4 mM of d-luciferin potassium salt (Promega) to confirm the expression of a functional luciferase by bioluminescence imaging using ChemiDoc MP (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... lactis CICC 6242 strain producing nisin A was extracted using a “Wizard Genomic DNA Purification” kit (Promega, Madison, USA). The nisA and nisRK genes were amplified through a polymerase chain reaction using the primers listed in Table 1 [29] ...
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bioRxiv - Plant Biology 2020Quote: ... First strand cDNA synthesis was carried out using 2 ug of total RNA and reverse transcription was performed using an oligo (dT) 15 primer and Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI, USA). qPCR was carried out using SYBR GREEN PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... oligo deoxythymidylic acid (oligo-dT) primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus Reverse Transcriptase (Promega, Madison, WI, USA). RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... PHH were infected with HepAD38-derived GTD virus at 500 viral genome equivalents per cell in maintenance medium supplemented with 4 % PEG 8000 (Promega, Madison, WI; V3011). For patient sera infections ...
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bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
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bioRxiv - Genomics 2020Quote: ... Five micrograms of RNA was reverse transcribed into cDNA using Moloney-murine leukemia virus reverse transcriptase and random primers (Promega, Madison, Wisconsin, USA) in a 20 μl reaction volume ...
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bioRxiv - Plant Biology 2022Quote: ... Subsequently 2 μg of RNA were used to perform reverse transcription in the presence of Moloney murine leukemia virus reverse transcriptase (Promega, Madison, WI, USA) after annealing with an anchored oligo(dT)18 primer as described by (Wirth et al. ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA from yeast strains grown in YPD media was isolated with the acidic hot phenol method 41 and treated with DNase (Promega) according to the manufacturers’ protocols ...
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bioRxiv - Cell Biology 2021Quote: ... was prepared from tachyzoites of RH or RH ΔKu80 (here referred as Δku80) strains using the Wizard SV genomic DNA purification (Promega) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Clonal line selection was then performed with newly acquired mutant strains before molecular verification by diagnostic PCR using GoTaq DNA polymerase (Promega) and multiple sets of primers (Tables S9 and S10) ...
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bioRxiv - Immunology 2022Quote: The genomic DNA of the 5 assessed M strains were isolated and purified using the Wizard Genomic DNA Purification Kit (Promega). The growing of strains ...
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bioRxiv - Genetics 2020Quote: ... Using the primers provided in Supplementary Table 3 we performed PCRs on 40 ng of genomic material extracted from wild type mosquitoes of the G3 strain using the Wizard Genomic DNA purification kit (Promega). The primers were modified to contain suitable Gibson assembly overhangs (underlined ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... retrovirus vector containing chick U6 promoter which expresses shRNA for gfp or Plzf was transfected into M/O chicken strain-derived embryonic fibroblast cells (CEF) cultured in 60-mm dish using Fugene HD transfection regent (Promega). CEF was cultured in DMEM-high glucose containing 10% FBS ...
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bioRxiv - Cell Biology 2022Quote: A full length TNY1 cDNA was amplified with primers TNY1-1F and TNY1-1R (Supplementary Table) from cDNA prepared using RNA from wild-type strain CC124 and inserted into pGEM-T easy vector (Promega) to generate pGEM-TNY1 ...
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bioRxiv - Microbiology 2023Quote: Genomic extraction for the WT C6706:str2 strain and each of the resistant mutants was performed using Wizard Genomic DNA Purification Kit (Promega). The concentration of DNA was quantified using Nanodrop spectrophotometer (ND-1000) ...
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bioRxiv - Microbiology 2023Quote: The N protein of SARS-CoV-2 B.1 D614G variant (NYI67-20 strain) was cloned into Nano-Luc vector pNLF1-N [CMV/Hygro] (N terminus) (Cat. N1351, Promega) as a fusion protein ...
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bioRxiv - Genetics 2023Quote: High molecular weight DNA was isolated from spleen tissue of a single male from each of the 11 imported Nachman wild-derived inbred strains using the Wizard DNA Purification Kit (Promega) or the Monarch HMW DNA kit (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Lactic acid production was assessed for each representative strain after 24 hours incubation in the same conditions as for the growth assessment using the Lactate-Glo Assay (Promega) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: The copy number of re-integrant strains was determined by extracting the genomic DNA and performing quantitative PCR (qPCR) using GoTaq polymerase (Promega) and a StepOnePlus real-time PCR machine (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA of all the pOXA-48 bearing strains was isolated using the Wizard genomic DNA purification kit (Promega, WI, USA), and quantified using the QuantiFluor dsDNA system (Promega ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA input was produced by midi prepping an overnight culture of 200 mL LB with the appropriate strain (Promega, A2492) and cleaned again using PCR cleanup (Promega ...
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bioRxiv - Microbiology 2022Quote: ... 2 × 106 cells of each strain were mixed completely with equal volumes of the BacTiter-Glo reagent (Promega Corporation, Madison, WI), followed by incubation at room temperature for 15 min in the dark ...
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bioRxiv - Microbiology 2023Quote: The cytotoxic activity on Hela cells of each bacterial strain was analyzed using the CellTiter-Blue® Cell Viability Assay (Promega) following manufacturer instructions ...