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Citations for Promega :
151 - 200 of 215 citations for Multi Analyte Recovery Spiking Std. 13C12 99% 200X Stock In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Firefly luciferase activity was then measured with BrightGlo luciferase reagent and a Glomax-Multi+ Detection System (Promega, Southampton, UK). Pseudovirus neutralization titres were expressed as the reciprocal of the serum dilution that inhibited luciferase expression by 50% (IC50).
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bioRxiv - Biophysics 2020Quote: ... containing 10% (vol/vol) FBS and 2% (vol/vol) GloSensor cAMP stock solution (Promega). The cells were then incubated for 2 hours at room temperature in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μL of cell lysate was transferred to 96-well plates and placed in a 96-well microplate luminometer (GloMax-Multi, Promega). 100 μL Luciferase Assay Reagent II and 100 μL Stop & Glo Reagent (both provided in the Dual-Luciferase Reporter kit ...
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bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays were performed on 20 μl of lysate or 1x Cell Culture Lysis Reagent in 96 well plates on the GloMax-Multi Detection System (TM297, Promega). 100 μl Luciferase Assay Reagent was added to wells ...
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bioRxiv - Microbiology 2019Quote: ... Plates were frozen for at least 30 minutes at −80°C before thawing and reading on a GloMax Multi plate reader (Promega). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated in the dark for 2 min and then read on a Glomax Multi+ Detection System (Promega) as above ...
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bioRxiv - Microbiology 2022Quote: ... was mixed 1:1 with cells seeded in 96-well plates for three consecutive days and the luminescence signal was quantified using the GloMax-Multi microplate reader (Promega). A list of all used sgRNA sequences and genotyping primers can be found in Table S4.
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... was performed following the manufacturer’s instructions and absorbance was measured at 490nm using a GloMax-Multi Plate Reader (Promega, E6521). Three independent experiments were performed ...
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bioRxiv - Cancer Biology 2021Quote: ... lactate assays were performed with culture media collected from each sample according to the manufacturer’s protocol and the optical density was measured at 450 nm using a microplate reader (GloMax®-Multi E7031, Promega)
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bioRxiv - Microbiology 2022Quote: ... and transferring the contents to a 96-well plate to which the appropriate substrate was added and assayed using a GloMax multi system (Promega).
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bioRxiv - Microbiology 2021Quote: ... from 12-72 hpt and luciferase activity was measured using the Dual Luciferase reagent kit and GloMax multi detection system (Promega). Firefly luciferase readings from the TBEV replicons were normalized to the Renilla values (pTK-Ren ...
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bioRxiv - Cancer Biology 2020Quote: ... Induction of ISRE-Luc reporter was evaluated by measuring Firefly and Renilla luciferase activity with dual-luciferase reporter assays on Glomax-Multi Detection System (Promega). The results were presented as relative light units (RLU) ...
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bioRxiv - Immunology 2021Quote: ... Fluorescence of FITC-dextran in serum was diluted to 1:10 with PBS and measured on a Promega GloMax Multi Detection System (Promega) at 490 nm excitation and 520 nm emission wavelengths ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was collected and protein concentration quantified using the 660 nm Protein Assay Reagent (Pierce) supplemented with 50 mM Ionic Detergent Compatible Reagent (Pierce) in a GloMax Multi Detection Plate Reader (Promega). After boiling in 1X Laemmli Sample Buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... and bacterial bioluminescence was measured in relative luminescence units (RLUs) every 24 h for up to seven days using a GloMax Multi Detection System (Promega) (35) ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase activity was measured with a GloMax®-Multi Detection system using the Dual-Glo® Luciferase Assay System (Promega). Results were analyzed using Excel (Microsoft ...
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bioRxiv - Genomics 2023Quote: ... Cells were harvested 24 hours after transfection and luciferase measured using Luciferase Assay System and the GloMax Multi-Detection System (Promega). For Verteporfin (VP ...
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bioRxiv - Molecular Biology 2024Quote: ... the amount of fluorescence of supernatant and solubilized cells was measured with a Glomax multi + detection system (Promega, WI, USA). The efflux capacity was calculated from the ratio of fluorescent intensity in the supernatant and the total fluorescent intensity from the supernatant and cell lysate ...
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bioRxiv - Biochemistry 2022Quote: ... the binding solution contains 1.0 µl of NanoLuc substrate stock solution (Promega, Madison, WI, USA). After addition to one well ...
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bioRxiv - Plant Biology 2022Quote: ... according to the instructions with the following modifications: stock oligo(dT)15 primers (C1101, Promega) concentration was 50 ng/μl and 0.5 μl Superscript II enzyme was used per reaction ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cells were lysed and the luciferase activities of Photinus pyralis and Renilla reniformis were measured using a GloMax®-Multi luminometer (Promega), following the instructions of the Dual-luciferase reporter assay system (Promega) ...
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bioRxiv - Genetics 2021Quote: ... The luciferin is then utilized in a firefly luciferase reaction to generate a bright luminescent signal determined as RLUs using a GloMax-Multi Detection System: Luminometer (Promega, USA). 2/ Colorimetric detection of senescence-associated β galactosidase following the manufacturer’s protocol (Cell Signaling #9860) ...
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bioRxiv - Genetics 2021Quote: ... Development and stop solutions were added followed by detection of absorbance at 450 on a Luminometer: GloMax-Multi Detection System (Promega, USA).
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bioRxiv - Synthetic Biology 2023Quote: ... Crude extract (10 μl) was mixed with 40 μl of LARII and FLuc activity was determined using a GloMax®-Multi Detection System (Promega) with a 2 s delay and a 10 s integration times ...
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bioRxiv - Cell Biology 2023Quote: ... incubated 3 h at 37°C and alkaline phosphatase (SEAP) activity determined measuring the absorbance at 600 nm using GloMax®-Multi Detection System (Promega).
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bioRxiv - Developmental Biology 2021Quote: ... 10 µL of a 0.1 mg/mL stock solution of trypsin (Promega Trypsin Gold MS grade) in 5 mM acetic acid was freshly diluted into a 140 µL solution of 10 mM NH4HCO3 to make the working digestion solution ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were diluted to 1M urea using a stock of 50mM ammonium bicarbonate and trypsin (Promega) was added at a 1:50 enzyme-to-substrate ratio and incubated overnight at 37°C shaking at 600 rpm ...
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bioRxiv - Microbiology 2021Quote: All virus stocks were produced by plasmid transfection of HEK 293T cells with Fugene 6 (Promega). Supernatants were harvested at 48h and 72h ...
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bioRxiv - Biophysics 2024Quote: ... Samples were diluted to 1M urea using a stock of 50mM ammonium bicarbonate and trypsin (Promega) was added at a 1:50 enzyme-tosubstrate ratio and incubated overnight at 37 °C while shaking at 600 r.p.m ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and luciferase/Renilla signal was quantified on a GloMax®-Multi+ dual injector luminometer using a Dual-Glo® Luciferase Assay system (Promega). To determine the effect of increasing the amount of Tbx2a co-transfected on luciferase expression we transformed the measure of luciferase/Renilla for each co-transfected reaction relative to the average measure of luciferase/Renilla of the same plasmid that was not co-transfected with Tbx2a ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 100 µL of the developed reagent was transferred to a solid white 96-well assay plate and fluorescence was read using a multi-modal plate reader (Ex 520nm/Em 580nm; GloMax Explorer, Promega, WI, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The resulting formazan precipitate was then dissolved by addition of 100 μL DMSO and quantified spectrophotometrically at 560nm using the GloMax Multi Microplate Multimode Reader (Promega, Madison, USA). Cell viability was expressed as a percentage relative to the negative control of untreated cell samples.
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bioRxiv - Immunology 2021Quote: ... and bacterial bioluminescence was measured every 24 h for up to 120 h with a GloMax Multi Detection System (Promega, Madison, WI). For the M.tb replication rate experiment ...
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bioRxiv - Synthetic Biology 2023Quote: ... lysed with 1X Passive Lysis Buffer then analysed using the Dual-Luciferase Assay kit on a GloMax multi+ plate reader (Promega, Southampton, UK).
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bioRxiv - Cancer Biology 2023Quote: ... 100 µL of cells were plated in opaque-walled (white) multi-well plates and 100 µL CellTiter-Glo (Promega, WI, USA, G7572) solution was added avoiding bubbles ...
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bioRxiv - Evolutionary Biology 2023Quote: ... post-injection using a Synergy HTX Multi-mode Microplate Reader (Bio Tek Instruments, Winooski, VT) and the Nano-Glo Dual Luciferase Reporter System (Promega, Madison, WI) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and DNA-concentration was determined using a QuantiFluor® dsDNA kit (detection limit: 50 pg/mL; sensitivity: 0.01 – 200 ng/µL) and Glomax® –Multi+ system (Promega, Madison, WI) with the blue fluorescence optical kit installed (Ex ...
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bioRxiv - Molecular Biology 2022Quote: ... Luciferase activity was analyzed using the Dual-Luciferase® Reporter Assay System and read with GloMax®-Multi Detection System (Promega, Madison, WI).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5 μL (20x working stock of NanoBRET Tracer K5 [20 μM] in Tracer Dilution Buffer (Promega N291B)) was added to each well with the exception of the “no tracer” control wells ...
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bioRxiv - Cancer Biology 2023Quote: ... mice received an intraperitoneal injection of 100 µl D-luciferin (stock of 40 mg/mL, Promega, E160). Mice were allowed to move freely for five minutes post injection prior to initiating anesthesia via isoflurane ...
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bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 bacterial artificial chromosome and human genome Promega #G1471). Absolute copy number of genomic stocks was determined using droplet digital PCR (Biorad QX600) ...
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bioRxiv - Systems Biology 2021Quote: ... Protein digestion was performed for 16 h at 37 °C with 0.1 µg of Trypsin/Lys-C mix (1 µL of stock; Promega) and stopped by lowering pH to 3 by the addition of 5 µL of 10% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... in DEPC-treated phosphate buffer (PB) with 0.5% Triton X-100 supplemented with RNasin (40Ul/µl stock, 5 ml/ml of buffer, Promega). We rinsed sections ...
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bioRxiv - Biochemistry 2022Quote: ... containing NanoLuc substrate was added to the living cells (40 µl/well, containing 1.0 µl of NanoLuc substrate stock from Promega). Thereafter ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in which donor luciferase luminescence was stimulated with the addition of furimazine (1/960 dilution from Promega manufacturer’s stock) in the additional absence or presence of the orthosteric agonist 10μM isoproterenol (final assay volume ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 3 nM of either XeARPP19 or various forms of ClyARPP19 (stock solutions at 1 μg/μL) were incubated in the presence of 62.5 units of recombinant bovine PKA (Promega) and 1 mM γS-ATP in a final volume of 30 μL of PKA Buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: gDNA was extracted from stationary phase evolved cocultures following revival from frozen stocks using a Wizard Genomic DNA purification Kit (Promega). DNA fragment libraries were constructed for samples from shaking WT-based cocultures A-F and NifA*-based cocultures M-R at generation ∼146 using NextFlex Bioo Rapid DNA kit ...
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bioRxiv - Cell Biology 2021Quote: ... cells were again washed with DPBS and incubated with 90 μL of a 1/1000 dilution of furimazine stock solution (Promega) in 0.1 % BSA/HBSS ...
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bioRxiv - Neuroscience 2020Quote: ... the culture medium was replaced with a CO2-independent medium containing 10% (vol/vol) FBS and 2% (vol/vol) GloSensor cGMP stock solution (Promega). The cells were then incubated for 2 h at 27°C in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... Fresh batches of ARPE-19 cells from a contamination-free stock that had been tested for mycoplasma (MycoAlert; Promega Inc.) were used every 6 to 8 weeks ...