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Citations for Promega :
51 - 100 of 5521 citations for Mouse Serine threonine protein kinase pim 2 PIM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: FAK1 kinase domain (AA393-698) (Promega) (25 μg ...
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bioRxiv - Molecular Biology 2022Quote: ... and ADP-Glo kinase assay (Promega), in accordance with the manufacturers’ instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and ADP-GloTM Kinase Assay (Promega). By measuring luciferase activity using the Ensight Multimode Plate Reader (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: ... using ADP-Glo Kinase Assay (Promega). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... The synthetized ATP was quantified using a luciferin/luciferase assay (Kinase-Glo Max Luminescence Kinase Assay, Promega) in a Beckman Coulter Paradigm plate reader ...
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bioRxiv - Molecular Biology 2023Quote: ... Experiments with varied activator concentration and screening were measured using the Kinase-Glo Luminescent Kinase Assay (Promega). Reactions were incubated at 25°C for 1 hour then reduced to 4°C in a thermocycler consisting of 50nM of recombinant VCP with or without 50nM UFD1/NPLOC4 in 50uL ATPase reaction buffer (25mM HEPES ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: To measure ATP bound to detergent purified P2X1 receptor a Kinase-Glo® Luminescent Kinase Assay (ProMega, USA) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... The assay was terminated with the addition of Kinase-Glo® Luminescent Kinase Assays reagent (Promega, Cat# V6711) and read in an EnVision Multilabel Plate Reader (Perkin Elmer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Bone Morphogenetic Protein 4 (BMP4, 10 ng/ml, Bio-Techne) plus phosphoinositide 3-kinase inhibitor Ly294002 (10 µM, Promega). After 42 h incubation at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... In brief: Full-length kinase ORF (Promega) cloned in frame with a NanoLuc-vector (as indicated in Table S8 ...
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bioRxiv - Biochemistry 2020Quote: ... In brief: Full-length kinase ORF (Promega) cloned in frame with a NanoLuc-vector (as indicated in Table SX ...
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bioRxiv - Neuroscience 2022Quote: ... a 10 μL kinase detection reagent (Promega) was added and incubated for 1 h at 25°C to convert the generated ADP to ATP ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.5ng thymidine kinase renilla luciferase reporter (Promega), 0.5-200ng empty or Vpx expressing pcDNA3.1 ...
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bioRxiv - Biophysics 2023Quote: The ADP-Glo lipid kinase assay (Promega) was used to monitor ADP production ...
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bioRxiv - Cell Biology 2024Quote: ... treated with Cdk5/p35 kinase (#V3271, Promega). 2μM (0,2 mg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... The in vitro kinase assay was performed using the Cyclin A2/Cdk1 Kinase Enzyme System (Promega, Madison, WI, USA), recombinant Cyclin B1/Cdk1 (SignalChem Biotech Inc. ...
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bioRxiv - Cancer Biology 2024Quote: ... the kinase reaction mixtures were collected after incubation and added into equal volume Kinase-Glo® regent (V6711, Promega) for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... Vmax and kcat values for given kinase substrates were determined using the ADP-Glo Kit system (Promega). In all cases reactions were carried out in 384 well plates at room temperature for 30 min with a total kinase concentration of 50 nM and 1 mM ultra-pure ATP ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The assay was carried out in 384-well plates using the ADP-Glo Kinase Assay kit (Promega), according to the published method [47] ...
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bioRxiv - Cell Biology 2022Quote: After extraction of proteins (see above) without proteinase and phosphatase inhibitors the Src activity was evaluated with ProFluor® Src-Family Kinase Assay (Promega).
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bioRxiv - Cancer Biology 2022Quote: MINK1 Kinase Enzyme System (Promega Cat No# V3911) and ADP-Glo™ Kinase Assay (Promega Cat No#V9101 ...
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bioRxiv - Physiology 2023Quote: ... was end-labeled by T4 polynucleotide kinase (Promega) using [γP32]-ATP (BioRad) ...
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bioRxiv - Biochemistry 2023Quote: ... For the ADP-Glo kinase assays (Promega, TM313), 5 μL reactions were prepared with varying concentrations of recombinant human IRF5 protein (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... 2° antibodies α- mouse (HRP detection, Promega, W4021). The mean intensity quantification of 4 independent experiments is shown in Figure 4 – supplement 1B ...
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bioRxiv - Biophysics 2021Quote: ... and the twitchin kinase domain lacking flanking tails (Kin) was measured by monitoring ATP hydrolysis using the Kinase Glo™ luminescent assay system (Promega). Assays were performed using 10 µg of protein sample in a 50 µL reaction volume ...
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bioRxiv - Plant Biology 2019Quote: ... Kinase activity was measured as ATP consumption by addition of 50 µl of Kinase-Glo® Plus Reagents (Promega; Fitchburg, WI), 10 min incubation at 30°C ...
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bioRxiv - Biochemistry 2020Quote: ... CellTiter-Glo 2 kit (Promega) using manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... 50 µ L of Kinase-Glo® reagent (Promega) was then added and incubated at room temperature for 10 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... we used the ADP-Glo™ Kinase Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ERK2 and ABL1 kinase assay were purchased from Promega. Enzyme ...
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bioRxiv - Cell Biology 2021Quote: ... SLK kinase enzyme system was purchased from Promega (#V4242). In vitro kinase assays were performed in 384-well low volume plates (Greiner #784075 ...
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bioRxiv - Developmental Biology 2023Quote: ... the ADP-GloTM Kinase Assay system (Promega, Madison, USA) was used ...
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bioRxiv - Genomics 2021Quote: ... Tris(2-carboxyethyl)phosphine (TCEP) and BCA protein assay kit were from Pierce and sequence-grade trypsin was from Promega. Rapigest and Sep-Pak C18 columns were from Waters and C18 Zip tips were from Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... The luminescence signal was developed for 20 min after adding 40 µl kinase detection reagent (kit) and measured with a GloMaxMulti plate reader (Promega). The average of three experiments performed in duplicate and normalized to PI3KC3-C1 activity were determined ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was incubated at 25°C for 30 minutes and the production of ADP was measured using the ADP-Glo kinase assay kit (Promega). Vmax was calculated after fitting the kinase activity data to the Michaelis–Menten equation ...
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bioRxiv - Cell Biology 2022Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Serially diluted inhibitor and NanoBRET™ Kinase Tracer K10 (Promega) at a concentration determined previously as the Tracer K10 IC50 (Supplementary Table 6 ...
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bioRxiv - Cancer Biology 2022Quote: ... and ADP-Glo™ Kinase Assay (Promega Cat No#V9101) were procured to perform the in vitro kinase assay ...
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bioRxiv - Microbiology 2019Quote: ... Amplified plasmid was then phosphorylated using T4 polynucleotide kinase (Promega) and self-ligated (T4 DNA ligase ...
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bioRxiv - Biochemistry 2023Quote: ... Serially diluted inhibitor and the corresponding NanoBRET Kinase Tracer (Promega) at a concentration determined previously as the Tracer IC50 (Table S3 ...
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bioRxiv - Biochemistry 2023Quote: VPS34 kinase activity was assessed via the ATP Glo (Promega) luminescence assay ...
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bioRxiv - Biochemistry 2024Quote: ... Kinase reactions were stopped with ADP-GloTM Reagent (Promega #V6930) followed by the addition of ADP GloTM Kinase detection reagent (Promega #V6930 ...
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bioRxiv - Molecular Biology 2021Quote: ... The radioactively labeled probe was first prepared with γ[32 P]ATP ATP and DNA oligonucleotides listed in Supplementary Table S5 with a T4 polynucleotide kinase kit (Promega), purified on MicroSpin G-25 (GE Healthcare ...
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bioRxiv - Genetics 2021Quote: ... and Pierce 660nm Protein Assay kit (Promega) was used for quantification ...
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bioRxiv - Neuroscience 2021Quote: ADP-Glo Kinase Assay reagents were from Promega (Madison, WI, USA). Reactions were performed according to manufacturer’s protocol with 1 µM full-length GST-tagged mouse Arc and 10 nM of human TNIK catalytic domain (Carna Biosciences ...
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bioRxiv - Cell Biology 2021Quote: AURKA autophosphorylation was evaluated using the ADP-Glo Kinase Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... and Kinase-Glo® Max were obtained from Promega (Madison, WI), Adenosine 5’-Triphosphatase from porcine cerebral cortex (Na+/K+-ATPase) ...
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bioRxiv - Biochemistry 2023Quote: ... Serially diluted inhibitor and NanoBRET Kinase Tracer K8/K9/K10 (Promega), at a concentration determined previously as the Tracer KD,app (Table S4) ...
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bioRxiv - Molecular Biology 2020Quote: ... The bacterial surface proteins with attached plasma proteins were released by limited proteolysis with 2 μG trypsin (Promega) /37 °C ...