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Citations for Promega :
101 - 150 of 4845 citations for Mouse E3 Ubiquitin Protein Ligase SIAH2 SIAH2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... RNAs were incubated with [5’-32P] pCp and T4 RNA ligase (Promega, M1051) O/N at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... DNA was extracted from mouse tail using genomic DNA extraction kit (Promega Wizard SV Genomic DNA Purification System ...
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bioRxiv - Biochemistry 2021Quote: ... Ligation into pMAD-AB vector was conducted using T4 DNA Ligase (Promega, standard protocol) overnight at 8 °C and a vector:insert ratio of 1:6 ...
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bioRxiv - Systems Biology 2021Quote: ... 100-200 ng of purified digestion was ligated by T4 DNA ligase (Promega M1801), and 2 μL of the product were transferred into 40 μL of TOPO competent cells (ice incubation for 20 min ...
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bioRxiv - Plant Biology 2022Quote: ... Digested DNA was ligated to adapters using T4 DNA ligase (Promega, Madison, WI, USA) and purified using Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... and then ligated for 16 h at 4 °C with T4 DNA ligase (Promega). After a further purification step ...
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bioRxiv - Molecular Biology 2019Quote: ... The final reaction volume contained 1-fold concentrated T4 ligase buffer (Promega, Madison, US). Prepared reaction mixtures (ligase buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... synthesized from Gene Universal (USA) at 4°C overnight (T4 DNA ligase, Promega, USA). Cys-loop mutation ligation products were transformed into DH5α (ThermoScientific ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was measured using Pierce™ BCA Protein Assay Kit and GloMax® Explorer Multimode Microplate Reader (Promega, Madison, WI, USA). Expression of OAT1 ...
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bioRxiv - Microbiology 2024Quote: ... difficile was mixed with 50 µL kit reagents (100-fold diluted LgBiT protein, 50-fold diluted substrate in kit buffer, Promega). Subsequently ...
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bioRxiv - Biophysics 2023Quote: ... ELISA plates were read on a GloMax plate reader (Promega, Wisconsin, US). Data were converted from concentration to total mass based on the volume of media/pellet ...
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bioRxiv - Biophysics 2021Quote: Some protein kinase assays were performed using the ADP-Glo™ assay kit (Promega), which measures the generation of ADP in a kinase reaction ...
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bioRxiv - Developmental Biology 2020Quote: ... [35S]-labeled Zc3h10 protein was produced by using TNT coupled transcription/translation kit (Promega). 20 ug of GST fusion proteins were incubated for 2 hrs at 4°C with in vitro translated Zc3h10 and glutathione sepharose beads ...
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bioRxiv - Cell Biology 2020Quote: ... The linear PCR products were circularised by ligation with T4 DNA ligase (Promega Cat#M180A) (O/N ...
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bioRxiv - Genetics 2021Quote: ... DNA ligation was performed using 5 μl HC T4 DNA ligase (Promega, 50-100 U) in 1X Ligation Buffer (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... pGEM®-T Easy Vector Systems and T4 DNA Ligase were from Promega (Madison, WI). Restriction enzymes and Q5® High-Fidelity DNA Polymerase were from New England Biolabs (Ipswich ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
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bioRxiv - Genetics 2023Quote: RNA was isolated from mouse liver using a Maxwell 16 LEV simplyRNA kit (Promega). For the American vs standard diet comparison strand-specific mRNA libraries were generated using the TruSeq Stranded mRNA protocol (Illumina ...
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bioRxiv - Cell Biology 2022Quote: HeLa cells were transfected with pEGFP-N1 or pEGFP-DFCP1 and co-transfected with pcDNA3-myc-Ubiquitin using Fugene6 (Promega 2023-11-06 Lot 0000425615). 24 h later the cells were washed twice in ice cold PBS and lysed (125 mM KAc ...
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bioRxiv - Plant Biology 2021Quote: ... Protein preparation and luciferase– substrate interaction were conducted with a Luciferase Assay Report Kit (Promega). Luciferase activity was measured by a microplate reader (Thermo Scientific Varioskan Flash) ...
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bioRxiv - Cell Biology 2023Quote: ... Test proteins were generated in vitro using the TNR quick-coupled transcription-translation kit (Promega). For radiolabeling ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA fragment ligation was done by using T4 DNA ligase as per the manufacturer’s protocol (Promega). Sequencing of the genetic constructs was performed by Macrogen Europe ...
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bioRxiv - Cell Biology 2022Quote: ... was digested with the corresponding enzymes and ligated with amplified genes using T4 DNA ligase (Promega).
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bioRxiv - Molecular Biology 2022Quote: ... Insertion of nucleotides into expression vectors was done with T4-DNA ligase (#M1804, Promega, Madison, USA) via EcoRI/NotI (#R6017/#R6435 ...
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bioRxiv - Microbiology 2024Quote: ... digested vectors and insert DNA fragments were ligated using T4 DNA Ligase (Promega, Madison, WI, USA) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA/Protein Purification Plus kit (Norgen, Thorold, ON, Canada) or the Maxwell 16 LEV simplyRNA purification kit (Promega, Madison, WI, USA) according to the manufacturers’ instructions ...
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bioRxiv - Biochemistry 2022Quote: ... [35S]-labeled proteins were synthesized in vitro via a coupled transcription/translation rabbit reticulocyte kit (Promega) incubated at 30°C for 90 min with [35S]Met/Cys metabolic labeling reagent (MP Biomedical ...
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bioRxiv - Biochemistry 2021Quote: Mitochondrial 35S-labeled precursor proteins were synthesized using the TNT SP6-coupled transcription/translation kit (Promega) and plasmid-vectors pSP64 containing the genes of interest ...
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bioRxiv - Plant Biology 2020Quote: ... and ligated with XbaI- and XhoI-digested pYL156 (Liu et al., 2002) using T4 DNA ligase (Promega). Ligations were transformed into house-made chemically competent E .coli DH10B ...
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bioRxiv - Biochemistry 2020Quote: ... The reverse transcriptase M-MLV RT as well as the T4 DNA ligase were obtained from Promega. The pGEM-T Vector kit was purchased from Promega ...
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bioRxiv - Synthetic Biology 2019Quote: ... ligated for 3 h at room temperature (RT) or overnight at 4°C using T4 ligase (Promega), and propagated in E.coli XL 10 Gold cells (Agilent) ...
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bioRxiv - Immunology 2021Quote: ... Ligation was performed on gel purified insert and digested plasmid with T4 Ligase (Promega, Madison, WI, USA) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The scFv gene was then ligated into pCSE2.6-hIgG1-Fc-XP vector (43) using T4 Ligase (Promega) and transformed into E ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.8 μl 100% DMSO, 2.5 μl T4 RNA ligase I – high concentration [NEB], 0.4 μl RNasin [Promega] ...
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bioRxiv - Molecular Biology 2021Quote: ... was digested with the same enzymes and ligated with the amplified receptors using T4 DNA ligase (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... 400nM of the annealed oligos were ligated with lng of digested crRNA backbone using T4 ligase (Promega) in lx T4 ligase buffer for 3hrs at RT.
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bioRxiv - Cancer Biology 2024Quote: ... Single-strand oligonucleotides were purchased from Sigma-Aldrich annealed and cloned into LRG2.1 (addgene, #108098) using the BsmBI endonuclease restriction site and T4_DNA ligase (Promega) for subsequent expression of the sgRNA together with EGFP fluorescent protein ...
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bioRxiv - Immunology 2020Quote: ... Total IgG and ANA ELISA plates were read using a GlowMax Discover Microplate Reader (Promega) on the absorbance setting at 450nm.
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bioRxiv - Immunology 2022Quote: ... ELISA plates were immediately read using a GloMax®-Multi Detection System microplate reader (Promega) at 450nm absorbance.
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bioRxiv - Microbiology 2020Quote: ... The KAN PCR amplicon with compatible ends was ligated into the plasmid backbone using T4 DNA ligase (Promega). The resultant pEXP5/Kan vector was linearized using XbaI and Butters gp31FLAG was ligated into the plasmid for transformation into chemically competent BL21 cells ...
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bioRxiv - Microbiology 2019Quote: ... and the resulting 889 bp fragment was cloned in the pBB-MCS1 vector using T4 DNA Ligase (Promega). This plasmid MCS1_dam was then digested with AatII and SacI enzymes to obtain a DNA fragment of 2194 bp containing a chloramphenicol resistance gene and the dam gene controlled by the Plac promoter ...
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bioRxiv - Microbiology 2022Quote: ... The resulting fragments were ligated to Nextflex adapters (Bio Scientific) with the use of T4 DNA ligase (Promega). The libraries were amplified using an AT-rich optimized KAPA protocol using KAPA HiFi HotStart ready mix (KAPA Biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... Ligation of the digested pGEM-T easy vector and 5’ fragments was carried out overnight at 4 °C using 0.5 µl (1.5 U) of T4 DNA Ligase (Promega). pGEM-T easy vector containing the 5’ fragment (5’-pGEM-T ...
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bioRxiv - Cell Biology 2022Quote: ... [35S]-methionine-labelled proteins were transcribed and translated using a TnT™ coupled transcription–translation kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: GTPase activity of the purified protein was measure using the Promega GTPase-Glo™ kit (Promega, V7681), as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA was extracted from mouse uterus and reverse transcribed into cDNA by reverse transcriptase kit (Promega). cDNA was amplified by gene specific primers ...
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bioRxiv - Cancer Biology 2020Quote: ... after isolating DNA from mouse tissue samples using a tissue DNA extraction kit (Maxwell® 16, Promega), the purified DNA was incubated and hydrolyzed enzymatically (EpiQuik ...
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bioRxiv - Genomics 2022Quote: ... DNA extraction from mouse liver tissue was performed using the Maxwell 16 Tissue DNA Purification kit (Promega) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... The digested vector (200 ng) was ligated with 1 µL of the oligonucleotides (0.45nmol/μl) using T4 DNA ligase (Promega) for 3 hrs ...