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Citations for Promega :
451 - 500 of 864 citations for Mouse Anti Respiratory Syncytial Virus Antibody RV4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... the cell viability of each infected virus variants was measured using CellTiter 96 Aqueous One solution Cell Proliferation Assay (Promega, USA). The relative cell viability was calculated by normalizing the absorbance value of treated virus samples against untreated virus samples ...
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bioRxiv - Microbiology 2019Quote: ... 4 × Promega Wizard® Minicolumns per replicate with a ratio of 0.5:1 virus concentrate to Wizard® PCR Preps DNA Purification Resin (Promega: A7170). The resultant extract was found to be inhibitory to all enzymatic reactions ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Microbiology 2022Quote: ... We performed duplicate serial dilutions using supernatants collected from the virus rescues and measured luciferase expression at each dilution using Bright-Glo Luciferase Assay System (Promega, E2610). Virus titers were calculated as relative light units (RLU ...
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bioRxiv - Molecular Biology 2023Quote: ... Produced pseudoviruses were titrated on HEK-293T-ACE2 by performing duplicate serial dilutions and virus titers were measured 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated at 37 °C for two days and the nLuc activity from the virus replication was measured by using Nano-Glo® Luciferase Assay System (Promega) following the manufacturer’s protocol using a plate reader (HT4 Biotek) ...
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bioRxiv - Immunology 2024Quote: ... The virus neutralization titers were determined by measuring the NLuc activity using the Nano-Glo Luciferase Assay System (Promega, Madison, WI) following the manufacturer’s conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... A polyclonal goat anti-mouse conjugated to horseradish peroxidase (1:5,000; 1:10,000; or 1:20,000; Promega, W4021) was used as the secondary antibody ...
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bioRxiv - Biophysics 2021Quote: ... followed by anti-rabbit alkaline phosphatase-conjugated secondary antibody (Promega, S3731, 1:5,000 in TBST) at room temperature for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody labeling was completed using anti-rabbit IgG-HRP conjugate (1:10,000; Promega W401B) or anti-mouse IgG-HRP conjugate (1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... The secondary antibody was a goat anti-Mus musculus IgG conjugated to horseradish peroxidase (Promega). After adding 100 μl of TMB product (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Rabbit and rat primary antibodies were detected with horseradish peroxidase (HRP) conjugated anti-rabbit (Promega) and anti-rat (Jackson ImmunoResearch ...
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bioRxiv - Biochemistry 2022Quote: ... and (ii) HALO tag was incubated with primary Anti-HaloTag® Monoclonal Antibody (Promega #G9211) diluted 1:1 000 with secondary antibody Anti-Mouse IgG (Fc specific ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibody labeling was completed using anti-rabbit IgG-HRP conjugate (1:10,000; Promega W401B) by incubating membranes for 2 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:10000) (Promega, Madison, WI, USA), diluted in 3% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... The blot was probed with 1:1000 dilution of anti-NLuc antibody (Promega cat#7000) and the bands detected by chemiluminescent detection.
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bioRxiv - Plant Biology 2020Quote: ... corniculatus plants was synthesized from 3 μg of total RNA using a Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) (Promega, WI, USA) and 100 pmol of random hexamers (Pharmacia Biotech) ...
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bioRxiv - Molecular Biology 2021Quote: cDNA synthesis for gene expression analyses was performed using 500 ng RNA using Moloney murine leukemia virus (M-MLV) reverse transcriptase and random hexamer oligonucleotide (both from Promega, Southampton, UK) using 500 ng RNA in a 25 µl reaction ...
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bioRxiv - Biochemistry 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (MMLV-RT) enzyme and recombinant RNasin® ribonuclease inhibitor were purchased from Promega (Madison, WI, USA). Evagreen 2X qPCR MasterMix was purchased from Applied Biological Materials Inc ...
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bioRxiv - Cell Biology 2021Quote: ... and 1-3μl aliquots were taken for reverse transcription (RT) using the Moloney murine leukemia virus RT RNase H (−) (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The resulting materials were used as template to generate cDNA using 100 U Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI), 0.45 μM N6 random hexamer primers (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from this concentrated virus sample using a modified protocol with the Wizard Genomic DNA Purification Kit A1120 (Promega, Madison, WI). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... virus replication was quantified by measuring Renilla luciferase activity (relative light units [RLU]) using Bright-Glo™ Luciferase Assay System (Promega, USA) 48 hours post-reinfection ...
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bioRxiv - Microbiology 2019Quote: ... Virus infectivity was quantified by measuring Renilla luciferase activity (relative light units [RLU]) using Bright-Glo™ Luciferase Assay System (Promega, USA) on the Tecan microplate reader (Tecan Infinite® 200 Pro ...
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bioRxiv - Cell Biology 2020Quote: ... Total 500 ng RNA was used to synthesize cDNA by using Moloney-murine leukemia virus (M-MLV) reverse transcriptase (Promega, Madison, WI). Diluted cDNA was used in each quantitative reverse transcription-PCR (qRT-PCR) ...
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bioRxiv - Plant Biology 2022Quote: ... cDNAs were synthesized from 1.5 μg of total RNA with oligo(dT) primers using Moloney Murine Leukemia Virus Reverse Transcriptase MMLV-RT according to the manufacturer’s instructions (Promega, Madison, WI, USA). RT-qPCR was carried out in a Chromo4 system (Bio-Rad ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37°C under 5% CO2 for 144 hour and virus cytopathic effect was measured using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Cat. # G9241) following the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... the amount of input virus was normalized to the HiBiT value measured by Nano Glo HiBiT lytic detection system (Promega, Cat# N3040)] ...
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bioRxiv - Microbiology 2023Quote: ... Samples where a 1 log decrease in RLU compared to the no-serum virus-only control was observed were then selected for cytotoxicity assay using Cell Titre Glo kit (Promega, Southampton, UK) and light microscopy to verify viability of cells prior to undertaking PVNA (data not shown) ...
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bioRxiv - Microbiology 2024Quote: ... followed by reverse transcription to complementary DNA (cDNA) using 30 enzyme units(U) of the Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) (Promega, WI, USA). The extension products were then analysed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... followed by three washes with TBS-T and 1 hr incubation in TBS-T + 1% milk + anti-mouse serum (1:10,000) (Promega). All steps were carried out at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Afterwards wells were washed again with PBS-T thrice and incubated with secondary mouse-HRP antibody (goat, Promega, Fitchburg, WI, United States) was added in a dilution of 1 ...
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bioRxiv - Plant Biology 2020Quote: ... First strand cDNA synthesis was carried out using 2 ug of total RNA and reverse transcription was performed using an oligo (dT) 15 primer and Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI, USA). qPCR was carried out using SYBR GREEN PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... oligo deoxythymidylic acid (oligo-dT) primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus Reverse Transcriptase (Promega, Madison, WI, USA). RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... PHH were infected with HepAD38-derived GTD virus at 500 viral genome equivalents per cell in maintenance medium supplemented with 4 % PEG 8000 (Promega, Madison, WI; V3011). For patient sera infections ...
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bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
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bioRxiv - Genomics 2020Quote: ... Five micrograms of RNA was reverse transcribed into cDNA using Moloney-murine leukemia virus reverse transcriptase and random primers (Promega, Madison, Wisconsin, USA) in a 20 μl reaction volume ...
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bioRxiv - Plant Biology 2022Quote: ... Subsequently 2 μg of RNA were used to perform reverse transcription in the presence of Moloney murine leukemia virus reverse transcriptase (Promega, Madison, WI, USA) after annealing with an anchored oligo(dT)18 primer as described by (Wirth et al. ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by a secondary anti-rabbit IgG antibody conjugated to alkaline-phosphatase (Promega, Madison, WI, USA). The membranes were blocked in TBST (150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... Subsequently anti-rabbit IgG horseradish peroxidase (HRP)-conjugated secondary antibody (Cat. No. W4011, Promega, Madison, WI) was used to detect the protein of interest with chemiluminescence reaction ...
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bioRxiv - Genomics 2022Quote: ... Sections were then incubated with a horseradish peroxidase-labeled anti-rabbit antibody (1:300, Promega, W401B) for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... 100 μl of 5,000-fold diluted Peroxidase AffiniPure goat anti-human IgG (H+L) antibody (Promega) was added into each well and incubated for 1 h at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... the membrane was incubated with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted 1:10.000 in PBS-T with 3% milk powder for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... blots with α-PRDX6 and α-PfNAPL were incubated with respective α-mouse and α-rabbit horse radish peroxidase (HRP)-coupled secondary antibodies (1:5000, Promega) for 1h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... Then wells were washed again three times with PBS-T (100 μl per well) and incubated with a secondary mouse-HRP antibody (goat, Promega, Madison, WI, USA) (50 μl per well ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequent to primary antibody removal chitin magnetic beads were washed three times with PBS-T and incubated with secondary mouse-HRP antibody (goat, Promega, Fitchburg, United States) 1:5000 in 100 μl sample buffer on a stirring wheel at room temperature for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and further incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse HRP conjugate (W4021, 1:10,000) and Anti-Rabbit HRP Conjugate (W4011, 1:10,000) were purchased from Promega (Madison, WI). Anti-COPI antibody was a gift from Charles Barlowe (Dartmouth Univ ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and were then incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...