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Citations for Promega :
301 - 350 of 1110 citations for Mouse Anti Cytomegalovirus Glycoprotein H gH 0861 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and reverse transcribed using M-MLV RNase H minus (Promega). cDNA products were purified on gel and amplified by PCR using the Phusion polymerase (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... 24 h later cells were transfected with Fugene HD (Promega) and constructs containing dCas9-VPR and gRNA vectors targeting the conserved DAR region (500ng plasmid DNA in molar ratios sgRNA:dCas9-VPR) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was obtained using M-MLV H-reverse-transcriptase (Promega) with a dT17 primer ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary HRP-conjugated antibodies were added at a dilution of 1:10,000 in 5% milk in TBST and incubated for 60 minutes at room temperature (Anti-Mouse: Promega #W402B; Anti-Rabbit: Promega #W401B). The Pierce SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Scientific #34577 ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then stained using the following primary antibodies overnight: mouse anti-β Galactosidase (1:1000; Promega), rabbit anti-Er81 (1:5000 ...
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bioRxiv - Cell Biology 2021Quote: ... A polyclonal goat anti-mouse conjugated to horseradish peroxidase (1:5000, 1:10000 or 1:20000; Promega) was used as secondary antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... The secondary antibody used was HRP-conjugated goat/rabbit/donkey anti-mouse IgG (1 : 10000, Promega, W402B).
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bioRxiv - Biophysics 2023Quote: ... prior to incubation with the primary anti-Halo-Tag antibody (Mouse monoclonal antibody, Promega, Cat. No.: G9211). For incubation with the primary anti-RBPJ antibody (Rat monoclonal antibody ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with a 1:100 dilution of primary mouse anti-NLuc antibody (Promega, cat#7000) in PBS overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and M-MLV Reverse Transcriptase (RNase H Minus, Point Mutant; Promega). Quantitative (q)RT-PCR reactions for target genes were prepared using specific primer (Oligomer ...
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bioRxiv - Immunology 2019Quote: ... and the luminescence after incubation with 5 µM coelenterazine H (Promega) were recorded using a Mithras LB940 reader (Berthold).
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bioRxiv - Microbiology 2021Quote: ... 48 h later cells were lysed with reporter lysis buffer (Promega) and assays were read on a FLUOstar Omega plate reader (BMF Labtech ...
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bioRxiv - Microbiology 2022Quote: ... 48 h later cells were lysed with reporter lysis buffer (Promega) and assays were read on a FLUOstar Omega plate reader (BMF Labtech ...
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bioRxiv - Developmental Biology 2020Quote: ... RNAs were reverse-transcribed using MMLV H(-) Point reverse transcriptase (Promega). Quantitative PCR was performed using the QuantiNova SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... template RNA was degraded by adding 2.5 U RNase H (Promega,) and 1µg RNase A (Pharmacia ...
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bioRxiv - Cancer Biology 2019Quote: ... Viability was assayed 72 h after plating using CellTiter-Glo (Promega).
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bioRxiv - Immunology 2020Quote: ... Infectivity was determined 72 h later by adding Bright-Glo (Promega) and measuring luciferase activity using a Synergy H1 plate reader (BioTek).
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bioRxiv - Molecular Biology 2023Quote: Luciferase activity was analyzed 48 h post-transfection using DualGlo (Promega), essentially as previously described (25) ...
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bioRxiv - Cancer Biology 2024Quote: ... Moloney Murine Leukemia Virus Reverse Transcriptase RNase H-Point Mutant (Promega) was used for cDNA synthesis ...
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bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used at the indicated concentrations: 1:10’000 mouse anti-β3-Tubulin (Promega, G712A), rabbit anti-p-ERK T202/Y204 (CST ...
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bioRxiv - Neuroscience 2022Quote: ... For immunostaining of the induced neurons following antibodies were used: anti-Tuj 1 (mouse; 1:2000, G7121, Promega), anti-MAP2 (chicken ...
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bioRxiv - Molecular Biology 2023Quote: ... A polyclonal goat anti-mouse conjugated to horseradish peroxidase (1:5,000; 1:10,000; or 1:20,000; Promega, W4021) was used as the secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies used in this study were 1:2,500-diluted HRP-conjugated anti-mouse IgG (Promega, #W4021) and anti-rabbit IgG (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibodies used included goat anti-rabbit IgG HRP-conjugated antibody (1:5,000, #7074S, CST) and goat/rabbit/donkey anti-mouse IgG HRP Conjugate (1:5,000, #W402B, Promega). The membranes for streptavidin blotting were blocked with 3% BSA ...
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bioRxiv - Plant Biology 2019Quote: ... during 4 h then with trypsin (Sequencing Grade Modified, Promega, Madison, WI) overnight ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 48 h later the cells were lysed in Reporter lysis buffer (Promega). Samples were then boiled in 5× sodium dodecyl sulphate (SDS ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was assayed 120 h after induction using CellTiter-Glo (Promega). Luminescence signals of induced wells were normalized to uninduced well data.
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with 5 μM coelenterazine h (Promega, Madison, WI, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μL BSA (1 mg/mL) and 2 μL buffer H (Promega) in reaction volume of 20 μL ...
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bioRxiv - Genetics 2019Quote: ... RNA expression levels were quantified after reverse transcription with MMLV H-(Promega) using the TaqMan gene expression system (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... and 72 h later viable cells were detected using CellTiter Glo (Promega). Compounds were tested in a 16-point dose response (10 µM highest final concentration and 2-fold dilutions) ...
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bioRxiv - Microbiology 2021Quote: ... After 48 h 40 μL/well of One-Glo-EX substrate (Promega) was added to the cells and incubated in the dark for 5-10 min prior to reading on a BioTek plate reader ...
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bioRxiv - Microbiology 2022Quote: ... 48-55 h after infection Bright-Glo Luciferase Assay System (Promega, E2610) was used to measure luciferase activity ...
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bioRxiv - Microbiology 2022Quote: ... 24 h post transfection the Dual-Luciferase® Reporter Assay System (Promega) was used according to the manufacturer’s manual ...
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bioRxiv - Neuroscience 2023Quote: ... Cellular NAD(H) levels were measured using NAD/NADH-Glo assay (Promega). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... After 16 h cells were lysed in 1x passive lysis buffer (Promega) and firefly luciferase activity was measured after addition of firefly substrate-containing buffer (20 mM Tricine ...
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bioRxiv - Immunology 2021Quote: ... followed by three washes with TBS-T and 1 hr incubation in TBS-T + 1% milk + anti-mouse serum (1:10,000) (Promega). All steps were carried out at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega) also used at a 1:10,000 dilution.
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bioRxiv - Microbiology 2021Quote: ... Samples were then incubated for 1h at room temperature with horseradish peroxidase-conjugated anti-mouse IgG secondary antibody (Promega) at 1:10,000 and peroxidase was detected using the Pierce chemiluminescence system (Pierce ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was a horseradish peroxidase polyclonal goat anti-mouse (from 1:5,000 to 1:10,000, depending on the primary antibody; Promega, W4021). Proteins were detected with the ECL chemiluminescence reagent (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse HRP conjugate (W4021, 1:10,000) and Anti-Rabbit HRP Conjugate (W4011, 1:10,000) were purchased from Promega (Madison, WI). Anti-COPI antibody was a gift from Charles Barlowe (Dartmouth Univ ...
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bioRxiv - Microbiology 2020Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega, #W4021) used at a 1:10,000 dilution.
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bioRxiv - Microbiology 2020Quote: ... the plates were washed with PBS-T five times and added with a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG antibody (Promega) in blocking buffer at 37 °C for 45 min ...
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bioRxiv - Cell Biology 2020Quote: ... or mouse (W4021; Promega) IgGs ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Molecular Biology 2019Quote: ... and reverse transcribed with Moloney murine leukemia virus reverse transcriptase RNase H(−) (Promega) using oligoT primer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The enhancer constructs were transfected 24 h after seeding using FuGENE6 (Promega, US) as the transfection reagent ...
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bioRxiv - Microbiology 2020Quote: ... was measured at 72 h post-infection using the Bright-Glo reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Acquorin was reconstituted by treating the seedlings with coelenterazine h (Promega, Beijing, China) in dark overnight at a final concentration of 10 μM ...
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bioRxiv - Microbiology 2020Quote: ... The product was subsequently reverse-transcribed using RNase H minus reverse transcriptase (Promega) at 42°C for 30 minutes and buffer was exchanged for DDM buffer ...