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Citations for Promega :
451 - 500 of 4252 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Homogenates were diluted 1/100 with the extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). Luminescence was measured with a SpectraMax Gemini XPS luminometer (Molecular Devices) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted from protoplasts after a 16h incubation in WI medium using 100 μl of 1 × passive lysis buffer (Promega). Luciferase activities were measured using the Dual Luciferase Reporter Assay kit (Promega ...
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bioRxiv - Microbiology 2020Quote: ... This was followed by in-gel digestion using 1:100 ratio enzyme:sample (w:w) using as enzyme sequencing grade modified trypsin (Promega, Madison, WI) overnight at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
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bioRxiv - Evolutionary Biology 2022Quote: ... the proteins were alkylated with iodoacetamide (100 mM for 1 h at room temperature in a dark chamber) and digested with trypsin (Promega) diluted in ammonium bicarbonate (0.1 M for 24 h at 37 °C) ...
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bioRxiv - Cell Biology 2022Quote: ... and dried in at 37 °C before overnight digestion with 30 μL trypsin (1:100 g/g, sequencing grade modified trypsin V5111, Promega) at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclei pellets were re-suspended in 100 µl of PBS containing 1 U/μl RNasin® Ribonuclease Inhibitors (Promega, # N2615) with or without 5 µg/µl of yeast tRNA (Thermo ...
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bioRxiv - Systems Biology 2019Quote: ... and dried in an oven at 37 °C) before overnight digestion with 20 μL trypsin (1:100 g/g, sequencing grade modified trypsin V5111, Promega) at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were harvested 36 h after transfection by lysing in 100 μl of 1× reporter lysis buffer (Promega, Madison, WI). Lysates were pelleted ...
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bioRxiv - Microbiology 2021Quote: ... The cytotoxicity of compounds (100 uM to 1 uM, 3-fold dilution) was examined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). Cell cytotoxicity data was normalized to DMSO control as 0% cell death.
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bioRxiv - Microbiology 2021Quote: ... resuspended in 50 μL (fLuc assays) or 100 μL (NanoLuc assays) of 1× Cell Culture Lysis Reagent (Luciferase Assay System; Promega) and incubated for 10 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... using one of the following antibodies against (all dilutions in PBS): HaloTag® polyclonal anti-rabbit (G928A, Promega, 1:100); ACTN2 monoclonal anti-mouse (EA-53 Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed with PBS and the cells were lysed with 1× reporter lysis buffer (100 µl per well; Promega). Following a freeze-thaw cycle ...
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bioRxiv - Neuroscience 2021Quote: ... The transfected cells were then treated with Aβ42 (1-4 μM) or calcitriol (100 nM) for 6 h before luciferase activity assay (Dual-Luciferase Reporter Assay, Promega). A 587-bp region of the human Cyp24a1 gene promoter that contains two VDRE motifs was cloned into the promoter-less luciferase expression vector pGL3-basic (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... of 50mM Tris-HCl pH 8.5 containing LysC (Wako Chemicals) at 1:100 (w/w) ratio and trypsin (Promega V5113) at 1:100 (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:100 in storage buffer and analyzed as indicated in the manufacturer’s protocol (LDH-Glo Cytotoxicity Assay, Promega, #J2381).
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bioRxiv - Cell Biology 2023Quote: ... Isolated proteins were digested with 1:50 w/w LysC (Wako Chemicals, cleaves at the carboxylic side of lysine residue) and 1:100 w/w trypsin (Promega, Sequencing-grade ...
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bioRxiv - Molecular Biology 2023Quote: ... The library preparation involved using 100 ng of genomic DNA spiked with 1% (w/w) of unmethylated lambda DNA from Promega. Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... For transient expression experiments each well was transfected with 100 ng of DNA using 1:3 ratio of FUGENE HD (#E2311, Promega). Twenty-four hours after transfection or doxycycline stimulation ...
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bioRxiv - Microbiology 2023Quote: ... 100 μL of these purified Se cells or 100 μL of the corresponding Se-Ai cocultures were mixed with 100 μL Nano-Glo Luciferase assay reagent (50:1 mixture of substrate:buffer, Promega N1110) in a 96-well plate ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing 20-30 pmol rhodopsin were mixed with 100-250 fmol BSA and cleaved with 1 μg trypsin/LysC mix (Promega) using the SP3 beads protocol described in [29].
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bioRxiv - Microbiology 2023Quote: For NanoBIT experiments 12,000 HeLa cells were seeded in 96-well black plates and each well was transfected with 100 ng of total DNA using 1:3 ratio of FUGENE HD (#E2311, Promega). Different amounts of plasmid DNA were used to achieve comparable expression of LgBiT/FLAG-tagged and SmBiT/V5-tagged proteins ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed with 100 μL PBS and lysed by addition of 20 μL 1 x Passive Lysis Buffer (Promega) to each well and nutated for 15 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... prepared by adding NanoDLR™ Stop & Glo® Substrate 1:100 into NanoDLR™ Stop & Glo® Buffer (Promega, USA), were added to the samples ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was digested overnight at 37 °C with sequencing-grade trypsin or chymotrypsin (Promega; 1:100 protease:protein weight ratio). After desalting on a C18 column (Pierce ...
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bioRxiv - Neuroscience 2019Quote: ... 100 µg of protein was then added to 100 µl of prewashed HaloLink resin (Promega G1914), which was prepared by washing and pelleting for 2m at 800xg 3x in wash buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 100 bp DNA ladder (Promega). Clones with an XbaI restriction site were then deep sequenced to confirm correct editing and zygosity ...
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bioRxiv - Neuroscience 2021Quote: ... and 100 nM of luciferin (Promega) at 36.5°C ...
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bioRxiv - Biochemistry 2019Quote: ... 100 μL of CellTiter-Glo (Promega) were added directly to 100 μL of cells ...
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bioRxiv - Physiology 2019Quote: ... 100 pg/μL of luciferase (Promega), an exogenous RNA not present in duck ...
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bioRxiv - Microbiology 2021Quote: ... 100 µL/well of OneGlo (Promega) was added and luminescent signal was read ...
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bioRxiv - Microbiology 2020Quote: ... either 100 μl Bright-Glo (Promega) was added to each well and incubated for 2 min or cells were lysed with Cell lysis buffer (Promega E1500 ...
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bioRxiv - Cancer Biology 2020Quote: ... and 0.1% Triton X-100 (Promega) at room temperature for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... 100 μL of HaloLink slurry (Promega) was centrifuged at 800 X g for 1 min to collect the resin ...
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bioRxiv - Neuroscience 2019Quote: ... 100 μL of 0.2% ProteaseMAX (Promega) was then added ...
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bioRxiv - Genetics 2020Quote: ... 50 mM (100×) Beetle luciferin (Promega) in water was mixed with 0.1% DMSO in water or 0.1 mM PF-670462 (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 100 U RNasin Ribonuclease Inhibitor (Promega), 40 U T7 RNA polymerase (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... and 100 μg trypsin (Promega v5280) was added and incubated for 16 hours at 25°C ...
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bioRxiv - Microbiology 2023Quote: ... and 100 μL NanoGlo substrate (Promega) in OptiMEM (Thermo ...
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bioRxiv - Molecular Biology 2024Quote: ... with 100 bp ladder (Promega, USA) or with a TapeStation 4150 using D5000 screen tape (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 U RNasin ribonuclease inhibitor (Promega) and 60 U T7 polymerase (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were lysed after 72 h (2+1 DIV for differentiations) using the Dual Luciferase Reporter kit (Promega, E1960) and luciferase activity was measured using a Victor3 (Perkin Elmer ...
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bioRxiv - Immunology 2021Quote: ... SIV pseudovirus Env construct was cotransfected with env-deficient backbone plasmid (pSG3ΔEnv) in a 1:2 ratio with transfection reagent FuGENE 6 (Promega) in HEK 293T cells according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... A partial trypsin digestion was performed to release proteins from the beads by using 80 μL of 2 M urea/50 mM Tris containing 1 mM DTT and 0.4 μg trypsin (Mass Spectrometry Grade, Promega) for 1 h at 25 C ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 mini tablet of Complete EDTA-free protease inhibitor and 2 µl RNAse inhibitor (RNAsin Plus RNase Inhibitor, Promega). RNA was extracted using the RNASpin Mini kit (GE Healthcare ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: Supernatants were incubated 1:2 with the substrate solution of the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega) for 30 min in the dark in a 96-well plate (Greiner) ...
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bioRxiv - Biochemistry 2020Quote: ... before being washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg μl−1 sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
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bioRxiv - Molecular Biology 2022Quote: ... Semiquantitative PCR reactions were carried out with 1–2 μL of cDNA and 0.2 μL of GoTaq DNA Polymerase (Promega) in a total reaction volume of 30 μL according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... HEK cells were transfected with the targeting plasmid and two helper plasmids (psPAX2 and pMD2.G) at a 2:1.5:1 molar ratio using ProFection (Promega). Two to three days later ...
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bioRxiv - Cell Biology 2022Quote: ... The gel pieces were rehydrated in trypsin solution (50 mM ABC pH 8.0, 1 or 2 µg trypsin per sample for proteome or BioID respectively, Promega) overnight at 37 °C ...