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Citations for Promega :
51 - 100 of 1488 citations for Monobenzyl Phthalate Unlabeled 100 Ug Ml In Methyl Tert Butyl Ether since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Unlabeled proteins were cell-free-synthesized using the TNT T7 Quick Reaction system according to the manufacturer instructions (Promega) and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... 30 mM Tris-HCl (pH 7), 1% Triton X-100, 1% NaDOC, 100 μg/mL cycloheximide (Applichem, Germany) and 30 U/mL RNase Inhibitor (Promega, United States)] ...
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bioRxiv - Developmental Biology 2022Quote: ... in the presence of 100 ng/ml B18R (Promega). The media was supplemented with 0.5 mM VPA ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM CaCl2 and 1 ug of sequence-grade trypsin (Promega) were added and incubation continued overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... and finally digested overnight with 1 ug Promega Trypsin Gold (Promega) per gel slice ...
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bioRxiv - Genomics 2019Quote: RNA samples (4 ug) were mixed with random hexamers (Promega, #C118A) and heated at 70 °C for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ug of protein per sample was digested with trypsin (Promega) overnight at 37°C using S-TRAP micro cartridges (Protifi LLC,) ...
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bioRxiv - Cell Biology 2021Quote: ... 100 U/ ml RNasin RNase inhibitor (Promega, cat. no. N2511), 2 mM vanadyl ribonucleoside complex solution (Sigma-Aldrich (Fluka BioChemika) ...
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bioRxiv - Cell Biology 2023Quote: ... as well as 100 U/ml RNAse Inhibitor (RNAsin; Promega). Cells were scraped ...
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bioRxiv - Genetics 2019Quote: ... 1 mM CaCl2 with 2 ug trypsin (Promega, Madison, WI, product V5111). Digest was stopped with formic acid ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with 4 ug Trypsin/Lys-C mix (Promega V5073) for 3-4 hrs at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900 μL of 0.35 M KCl (10 mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Microbiology 2020Quote: ... treated with 1 μL Proteinase K (100 μg/mL final; Promega) incubated at 37°C for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: Proteins were digested on-bead with 0.5 ug Sequencing Grade Modified Trypsin (Promega) overnight at 37°C with agitation ...
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bioRxiv - Genomics 2019Quote: Linearized plasmid (2.5 ug) was added to a mixture containing transcription buffer (Promega), 10 mM DTT ...
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bioRxiv - Genomics 2020Quote: ... 0.02 ug of the pRL-CMV Renilla Luciferase plasmid (Promega Accession No. AF025843) was co-transfected with firefly luciferase plasmids ...
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bioRxiv - Microbiology 2022Quote: ... Seventy five ug of each lysate were digested with sequencing-grade trypsin (Promega) 1:100 ratio for 12 hours at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 0.5 ug RNA using M-MLV Reverse Transcriptase (Promega, M3681) and oligo d(T)15 following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... LUC activity was measured with 20 mL of protein sample and 100 mL of LUC assay reagent (Promega). Promoter activation was calculated as (rate of MUG accumulation / LUC luminescence ...
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bioRxiv - Immunology 2021Quote: ... after added 100 mL of Caspase-Glo 3/7 reagent (Promega, USA), the plate was gently shaken at room temperature for 2 h and the luminescence was measured by a plate-reading luminometer.
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bioRxiv - Biochemistry 2022Quote: ... and one was treated with 100 µg/mL of RNase A (Promega) at 37 °C for 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.12 mM EDTA and 100 µg/mL RNAse A (Promega A797c, Promega) and incubated 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.12 mM EDTA and 100 µg/mL RNAse A (Promega A797c, Promega) and incubated 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and w/wo 100 ng/mL Nerve Growth Factor 2.5S (Promega, #G5141).
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bioRxiv - Molecular Biology 2019Quote: ... sliced and dehydrated twice using 50% ACN followed by 100% ACN and the proteins digested with ~100 µl of Trypsin solution (10 µg/ml, Promega) in 25 mM ammonium bicarbonate by incubating overnight at 37° C ...
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bioRxiv - Neuroscience 2020Quote: ... in DEPC-treated phosphate buffer (PB) with 0.5% Triton X-100 supplemented with RNasin (40Ul/µl stock, 5 ml/ml of buffer, Promega). We rinsed sections ...
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bioRxiv - Microbiology 2020Quote: ... 1.0 ug of total RNA was reverse-transcribed with ImProm-II reverse-transcriptase (Promega, USA) and random hexamers (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... 30 ug of the supernatant was assayed for luciferase activity using luciferase assay substrate (Promega).
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bioRxiv - Cell Biology 2023Quote: cDNA was synthesized from 2 ug of total RNA using GoScript Reverse Transcriptase (Promega, #A5003) with random hexamers according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples eluted with biotin were digested in-solution with 1 ug Trypsin/LysC (Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 5 mM MgCl2, 1.5 mM KCl, 100 μ g/ml cycloheximide, 1mM DTT, 200 U/ml RNase in from Promega, 0.5% Sodiumdeoxycholate ...
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bioRxiv - Molecular Biology 2023Quote: ... penicillin (100 U/ml) and streptomycin (100 μg/ml) in the presence or absence of compounds and HaloTag NanoBRET 618 Ligand (Promega) for 4 h ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 μg ml-1 cycloheximide, and 20 U ml-1 RNase inhibitor [Promega].) Gradients were centrifuged 36,000 rpm for 2.5 h in a SW41 Ti rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... Using 100 ml of Luciferase Assay Reagent II (LAR II) (Luciferase Assay Reagent, Promega) and ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.5) containing 1% Tween20 and 100 U/ml RNasin Plus RNase Inhibitor (Promega). After centrifugation at 15,000 g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 µL of nuclease-free water with RNAsin (100 U/mL) (Promega, Madison, WI) was added and incubated at 95°C for 1 min ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with 2 ug of pQCXIN ERAAP-dsRed using Fugene HD transfection reagent (Promega, E2311) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Biotinylated sAB-K29 (100 µg) was incubated with 1 mL streptavidin-magnetic beads (Promega, Z5482) prewashed three times with PBS buffer in a thermo shaker for 3 h at 25 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Bisulfite conversion was performed using the methyl edge bisulfite conversion system exactly as described by the manufacturer (Promega, USA). Four overlapping PCR primers (Bs-1F/1R ...
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bioRxiv - Cell Biology 2022Quote: ... 2.0 ug DNA of expression plasmids were mixed 150 μL Opti-MEM and transfected using FuGENE HD (Promega) at a DNA to FuGENE HD ratio of (1:3) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were transiently transfected with 0.1 ug of an individual plasmid with Fugene HD reagent (Promega, Madison, WI). 48 hours later ...
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bioRxiv - Plant Biology 2021Quote: ... 2 ug of RNA were reverse transcribed using a reverse transcription kit (GoScript™ Reverse Transcription System, Promega). The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 µl containg 5 μL SYBR-green master mix (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... 293T cells were transfected in 12 well plates with 1 ug of pcDNA3.4 POLGARF or pcDNA3.4 Timer (as a negative control) with Fugene transfection reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: 300 × 105 HEK 293T cells were split onto 6 well plates and the next day were mock transfected or transfected with 1.5 ug pTwist-CMV-SLC46A2-exFLAG using 4.5 uL FuGene6 transfection reagent (Promega). 24 h after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... 1M Tris-HCl was added to adjust the pH and 5 μL trypsin (2.5 ug, Promega Sequence Grade) was added and the samples digested overnight at 37℃ ...
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bioRxiv - Genomics 2020Quote: ... 3 ug of pCAG-NLS-Bxb1 was diluted in 250 uL of OptiMEM and 6 uL of Fugene (Promega). On day 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... The released peptides were then transferred over to a new tube and a 4 h digest using 0.5 ug mass spectrometry-grade trypsin (V5111, Promega) at 37 C for 4 hours was conducted ...
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bioRxiv - Developmental Biology 2023Quote: ... with 1 ug of RNA and RT-qPCR reactions were performed using GoTaq® qPCR Master Mix (A6002 - Promega). Analyses were carried out in the QuantStudio™ 5 Real-Time PCR System ...