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Citations for Promega :
1 - 50 of 2731 citations for Mitochondrial dimethyladenosine transferase 1 TFB1M Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... by a terminal Deoxynucleotidyl transferase (Promega, M1875) with a specificity of 2.5 x 106 cpm/ml (C-Fos ...
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bioRxiv - Cell Biology 2021Quote: ... and 30 units of terminal deoxynucleotidyl transferase (Promega) in 20 μl of transferase buffer at 37°C for one hour The reaction was halted by incubation at 70°C for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... and the mitochondrial ToxGlo™ (Promega) assay were used accordingly to the manufacturer’s instruction ...
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bioRxiv - Genetics 2021Quote: Terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining (Promega) was used to visualize fragmented gDNA within paraffin-embedded RV tissue sections ...
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bioRxiv - Microbiology 2021Quote: ... Mitochondrial toxicity assay was performed using the “Mitochondrial ToxGloTM Assay” kit according to the manufacturer’s instructions (Promega). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Cytosine tails (C-tails) were added to 1 µg sheared DNA using terminal deoxynucleotidyl transferase (TdT, Promega, Madison, WI). Transposon containing fragments were amplified in a PCR containing DNA from the TdT reaction as template and primers specific to the ColE1 site on the 5’ end of the transposon ...
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bioRxiv - Microbiology 2024Quote: ... Poly-C-tails were added to 1 µg of each end-repaired sample in duplicate using Terminal Transferase (Promega) with a ratio of 9.5 mM dCTP to 0.5 mM ddCTP to limit chain length and DNA Duplicate reactions were combined and purified with SPRIselect beads ...
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bioRxiv - Cell Biology 2022Quote: ... terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, Promega, Madison, WI, USA), immunohistochemistry (IHC) ...
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bioRxiv - Immunology 2023Quote: ... ATP was measured with Mitochondrial ToxGlo Assay kit (Promega) and followed the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... using the DeadEnd Colorimetric TUNEL (Terminal transferase-mediated dUTP nick-end labeling) system (Promega).
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bioRxiv - Synthetic Biology 2019Quote: ... Poly-C tails were added to the fragmented DNA using terminal deoxynucleotidyl transferase (Promega). Then ...
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bioRxiv - Cell Biology 2022Quote: ... ATP levels were assayed using the Mitochondrial ToxGlo™ Assay kit (Promega) as per manufacturer’s instructions on a FLUOstar OPTIMA (BMG LABTECH ...
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bioRxiv - Physiology 2019Quote: ... Cytotoxicity and ATP generation were measured using mitochondrial ToxGlo assay (G8000) from Promega as per the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: Terminal deoxylnucleotidyl transferase dUTP nick end labelling (TUNEL) assay (Dead End Fluorometric Tunel Assay, Promega corporation, Madison, USA) was performed 3 days after the UUO ...
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bioRxiv - Immunology 2020Quote: ... TUNEL (terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling) staining was performed using the DeadEnd kit (Promega, PRG7130) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Paraffin tissue sections mounted on glass slides were used for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, Promega) and 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2022Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was carried out with commercial kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) TUNEL assays were performed with the Deadend Fluorometric TUNEL system (Promega), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) staining was assessed using the DeadEnd Fluorometric TUNEL System (Promega #G3250) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... were made by end-labeling one primer of a complementary pair (primers listed in Table 2) using 20 μM Cy5-dCTP and Terminal deoxynucleotidyl Transferase (TdT; Promega). Complementary primers (one labeled with Cy5 and the other unlabeled ...
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bioRxiv - Microbiology 2020Quote: ... were made by end-labeling one primer of a complementary pair (primers listed in Table S3) using 20 μM Cy5-dCTP and Terminal deoxynucleotidyl Transferase (TdT; Promega). Complementary primers (one labeled with Cy5 and the other unlabeled ...
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bioRxiv - Cancer Biology 2022Quote: GSH/GSSG measurement was performed by a glutathione S-transferase (GST) enzyme coupled reaction using (GSH/GSSG Glo kit, Promega). Cells (2×104 ...
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bioRxiv - Microbiology 2022Quote: Flag-Trim69 was cloned downstream of a gluthathione-S-transferase (GST) in the pTKPL vector (an in-house derivative of the vector pGEXTK, Promega) and purified from bacterial lysates on glutathione agarose beads (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... The in situ detection of fragmented genomic DNA was performed according to the DeadEnd colorimetric TUNEL (Terminal transferase-mediated dUTP nick-end labeling) system (Promega). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... were then added to the 3’ ends of all fragments by incubation of 3 µg of size-selected DNA fragments with the recombinant terminal deoxynucleotidyl transferase (rTdT, 30 U/µL, Promega) at 37°C for 1H ...
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bioRxiv - Molecular Biology 2021Quote: ... pancreatic sections were submitted to terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay using a DeadEnd Fluorometric TUNEL system kit (Promega) according to the manufacturer’s instructions ...
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Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtypebioRxiv - Neuroscience 2021Quote: ... Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) assays using in situ cell death detection kit (Promega). All images were acquired using a Zeiss LSM710 confocal microscope and Zen software (Zeiss).
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bioRxiv - Cell Biology 2021Quote: ... and apoptosis was measured using the DeadEnd Fluorometric terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) system (Promega, #G3250). Images from each islet graft were obtained using an EVOS FL Autofluorescence microscope (Life Technologies) ...
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bioRxiv - Plant Biology 2022Quote: ... Nick-end labeling of nuclear DNA fragmentation mediated by Terminal Deoxynucleotidyl Transferase (TdT) was performed following the instructions of DeadEnd™ Fluorometric TUNEL System (Promega). Sections were stained in 1 μg/ml propidium iodide (PI ...
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bioRxiv - Cell Biology 2022Quote: ATP levels were estimated using the ATP detection reagent of the Mitochondrial ToxGlo Assay kit (Promega). 50 μg mitochondria from WT and deletion strains grown at 30°C in YPG media were lysed by two times snap freezing the mitochondria in liquid nitrogen followed by thawing on ice ...
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bioRxiv - Biochemistry 2021Quote: Mitochondrial 35S-labeled precursor proteins were synthesized using the TNT SP6-coupled transcription/translation kit (Promega) and plasmid-vectors pSP64 containing the genes of interest ...
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bioRxiv - Cell Biology 2021Quote: ... Beta cell death was determined in islet graft sections stained for insulin or glucagon using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method (Promega, Madison, WI) (20 ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: Programmed cell death was determined in retinal cryosections by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) using the DeadEnd Fluorometric TUNEL system following the manufacturer’s instructions (Promega, Madison, WI, USA). Next ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cytotoxicity and ATP Production were measured with the use of a Mitochondrial ToxGlo™ Assay Kit (Promega), as described in (Weatherly et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 μg of mitochondrial protein in triplicate was incubated in GTPase Buffer (Promega #V7681; Madison, WI, USA) with 10 μM GTP and 1 mM DTT ...
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bioRxiv - Physiology 2021Quote: ... terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was carried out by using the DeadEnd Fluorometric TUNEL System (Promega, Fitchburg, Wisconsin, USA) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... The level of ATP under different conditions was determined using the Mitochondrial ToxGlo™ Assay from Promega (UK), following the protocol provided by the manufacturer.
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bioRxiv - Biophysics 2020Quote: ... antibodies followed by a 1-hour incubation with the corresponding HRP-conjugated secondary antibodies (1:2000, Promega) at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Total cell and mitochondrial free cholesterol and cholesterol ester contents were measured using the Cholesterol/Cholesterol Ester-Glo Assay Kit (J3190, Promega) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2021Quote: ... were then used to amplify a 443 bp region of the mitochondrial cox1 gene using GoTaq Hot Start Master Mix (Promega). Each 50 µL reaction contained 500 nM each primer and cycling conditions were as described above ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used are anti-HaloTag monoclonal antibody (Promega, G921A, 1:1000), and polyclonal rabbit antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies used: ßgal (1:100, Promega), pMad (1:800 ...
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bioRxiv - Neuroscience 2022Quote: ... The standard curve was generated by cloning a short 100-bp sequence of mitochondrial 16S rRNA gene into pGEM®-T Easy Vector (Promega). The 16S_rRNA gene sequence was amplified with Taq Polymerase (Supreme NZYtech II master mix ...
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bioRxiv - Neuroscience 2021Quote: The remodeling of neuron isolated mitochondria was evaluated according to a published protocol,[54] by measuring the changes in the absorbance of the mitochondrial suspensions at 540 nm using a GloMax Discover multimode plate reader (Promega, Italy). Briefly ...
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bioRxiv - Cancer Biology 2019Quote: Mitochondrial activity of C-33A cells was measured with the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI). Cells were seeded in 96-well plates (5000 cells/well ...
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bioRxiv - Immunology 2019Quote: ... Secondary antibody (α-Goat HRP, Promega, 1:10,000). The blot was developed using ECL Western Blotting Substrate (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used included alkaline phosphatase-conjugated anti-rabbit IgG antibody (1:1,000, #S3738, Promega) and anti- mouse IgG antibody (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000, Promega). Chemiluminiscent signals were detected with an ECL Prime Western blotting detection kit (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).