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Citations for Promega :
201 - 250 of 4305 citations for Microtubule Associated Protein 1 Light Chain 3 Gamma MAP1LC3C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... with a 1:50 ratio of purified protein to Endoglycosidase H (Endo H) (Promega). After treatment ...
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bioRxiv - Immunology 2021Quote: ... The antibodies were purified from the culture supernatant 4 – 6 days later using protein G magnetic beads (Promega #G7472). Purified antibodies and antibody elution buffer (5 parts elution buffer (100 µM glycine-HCl ...
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bioRxiv - Immunology 2019Quote: ... from C57BL/6J or MD4 mice that had been pre-treated with CK-689,CK-666 were added to COS-7 APCs expressing either the single-chain anti-Ig,𝜅 surrogate Ag or mHEL-HaloTag that was labeled with HaloTag tetramethylrhodamine ligand (Promega, #G8251). After the indicated times at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Polymerase Chain Reaction (PCR) targeting recA was performed in 50 µl volumes using 10 µl of 5X buffer (Promega, Madison, WI, USA), 7 µl of 25 mM MgCl2 (Promega ...
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bioRxiv - Genomics 2021Quote: ... cells were transfected in a 3:1 ratio of FuGENE® HD Transfection Reagent (Promega cat # E2311) and plasmid DNA (pcDNA3.1-HMGB1P1-3xHA ...
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bioRxiv - Genomics 2023Quote: ... the final selection was performed by 3% agarose electrophoresis (80 V, 2 h, 1 kB Promega ladder). The selected clones were preincubated in growth medium (15 ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Caspase-3 and -7 activity was measured by Caspase-Glo 3/7 Assay (Promega) according the manufacturer’s instructions using a BioTek Synergy H1 plate reader.
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bioRxiv - Cancer Biology 2021Quote: ... Caspase 3/7 activity was determined with the Caspase-Glo 3/7 assay (Promega) and caspase activity was normalized to cell number by performing the CellTiter-Glo Luminescent Cell Viability Assay on the duplicate plate.
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bioRxiv - Cancer Biology 2022Quote: ... Caspase 3/7 activity was determined with the Caspase-Glo 3/7 assay (Promega), and caspase activity was normalized to cell number by performing the CellTiter Glo Luminescent Cell Viability Assay on the duplicate plate ...
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bioRxiv - Cancer Biology 2022Quote: ... caspase-3 and -7 activities were measured by Caspase-Glo 3/7 assay (Promega) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2020Quote: ... Caspase-3/7 activities were measured using Caspase-Glo 3/7 Assay kits (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 3 μg of purified RNA was incubated with 3 units of RQ1 DNase (Promega) at 37°C for 30 mins ...
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bioRxiv - Bioengineering 2021Quote: Caspase 3/7 activity was measured using the Caspase-Glo 3/7 assay (Promega). Caspase 3/7 assay solution was mixed 1:1 with αMEM without phenol red (ThermoFisher Scientific ...
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Class IIa HDACs inhibit cell death pathways and protect muscle integrity in response to lipotoxicitybioRxiv - Cell Biology 2023Quote: ... caspase 3 activity was measured using Caspase-Glo 3/7 assay (Promega, Alexandria, Australia). Quantification of viable cells was performed by staining with 7-aminoactinomycin D (7-AAD ...
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bioRxiv - Molecular Biology 2021Quote: ... protein digestion was performed by 1 µg of Lys-C (Wako) at 37 °C for 3 h following 1 µg of trypsin (Promega, Madison, WI, USA) at 37 °C for 16 h ...
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase 3/7 Glo (Promega) was used to measure caspase 3/7 cleavage ...
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bioRxiv - Biochemistry 2022Quote: ... 3 units/mL RNasin (Promega). Depending on the experiments (see results) ...
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bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...
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bioRxiv - Microbiology 2023Quote: ... and the luciferase relative light unit (RLU) of the lysates was measured using the luciferase assay system (Promega) and Polerstar optima microplate reader (BMG BioLabtech ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were 1:5,000 HRP-conjugated anti-rabbit (W4011; Promega) or mouse (W4021 ...
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bioRxiv - Biochemistry 2019Quote: ... and HRP-conjugated rabbit anti-mouse antibody 1:10,000 (Promega, W4028). The plate was developed with 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid ...
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bioRxiv - Neuroscience 2021Quote: ... JF-549 or Halo-tag antibody (1:500, Promega, mouse monoclonal) and measuring the newly synthesized actin (in most cases ...
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bioRxiv - Neuroscience 2021Quote: ... Immunofluorescence utilizing primary antibodies directed against βIII-tubulin (1:1000, Promega) and secondary antibodies Alexa Fluor-488 (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... Washes and incubation of HRP-conjugated secondary antibodies (1:5000, Promega) were performed in PBST (0.05% Tween 20 in PBS) ...
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bioRxiv - Genetics 2020Quote: ... Alkaline phosphatase-conjugated secondary antibodies were used at 1:10000 (Promega). Western blot band intensity was analyzed with ImageJ Gel quantification tool.
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used were anti-p75NTR (Promega, Cat: G323A, 1:300) and anti-GAPDH (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used: HaloTag mAB (Promega, G9211, 1:500), GAPDH (Cell Signaling 2118 ...
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bioRxiv - Biochemistry 2023Quote: ... The following primary antibodies were used: NanoLuc (N7000, Promega, 1:1000), β-ACTIN (#4970S ...
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bioRxiv - Plant Biology 2024Quote: ... and HRP-conjugated secondary antibody (Promega, Cat# W4011, 1:4,000 dilution).
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bioRxiv - Biochemistry 2021Quote: ... Phospho-Tyrosine specific antibodies (anti P-Tyr674/675 from Cell Signalling 1:3000) and anti-p75 intracellular antibody (Promega) were used ...
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bioRxiv - Microbiology 2019Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega) also used at a 1:10,000 dilution.
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bioRxiv - Genetics 2022Quote: ... membranes were washed and incubated with the appropriate HRP conjugated secondary antibody (1:3,000 anti-rabbit W401B antibody from Promega), and the reaction was finally visualized with the Western Blotting Luminol Reagent (Santa Cruz Biotechnology) ...
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bioRxiv - Microbiology 2020Quote: ... caspase-3/7 activity was quantified using the Caspase-Glo-3/7 assay system (Promega). Luminescence was quantified using plate reader Synergy H1 Hybrid Reader (BioTek).
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bioRxiv - Cancer Biology 2021Quote: ... Caspase-3/7 activity was measured using Caspase-Glo 3/7 Assay Systems (G8091, Promega) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... caspase-3/7 activity was quantified using the Caspase-Glo-3/7 assay system (Promega). Luminescence was quantified using plate reader Synergy H1 Hybrid Reader (BioTek).
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bioRxiv - Immunology 2019Quote: ... RAC2 3’UTR-: 5’-TTGCGGCCAGCGGCCGCTCTCCAAACTTGAATCAATAAATTT-3’ and inserted into a dual luciferase psiCHECK2 backbone (Promega). RAC2 mutant 3’UTR constructs were generated using Infusion HD cloning kit (Clontech ...
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bioRxiv - Immunology 2021Quote: Caspase 3/7 activity was measured using Caspase-Glo® 3/7 Assay (Promega, G8090) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase 3/7 activity was assessed using the Caspase-Glo 3/7 assay reagent (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The intracellular caspase-3/7 activity was measured using Caspase-Glo 3/7 Assay (Promega). For this ...
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bioRxiv - Cell Biology 2022Quote: ... Caspase 3/7 activity was measured by adding Caspase-Glo 3/7 Assay Reagent (Promega), incubating for 45 min at room temperature and measuring luminescence in an EnVision 2104 Multilabel plate reader (PerkinElmer).
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bioRxiv - Microbiology 2022Quote: ... for Calu-3 cells and CellTiter-Glo 3-D Cell Viability Assay Kit (Promega, Germany) for hBAECs according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Caspase-3/7 activity was detected using the Caspase-Glo 3/7 Assay Kit (Promega) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: Caspase 3/7 cleavage activity was measured by Caspase-Glo 3/7 Assay Kit (Promega). 1 ×104 cells/well were plated in a pre-coated 96-well plate ...
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bioRxiv - Biophysics 2021Quote: ... The 2725-nt RNA molecules were synthesized by polymerase chain reaction (PCR) amplification of the plasmids followed by in vitro transcription using the T7 RNA polymerase (Promega, Fitchburg, WI, USA). The RNAs contain a 1206-nt upstream sequence ...
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bioRxiv - Plant Biology 2021Quote: ... Protein expression was confirmed by Western blot with Anti-HaloTag monoclonal Ab (1:2000) (Promega). Cy3-labeled and unlabeled dsDNA probes were generated with oligonucleotides listed in Supplemental Table 5 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins in each well were digested with 1 μg of sequencing-grade trypsin (Promega Inc.) in 75 μl of 50 mM ammonium bicarbonate and incubated overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... On-bead protein digestion was performed by adding 1 µg of sequencing grade trypsin (Promega) to the samples ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested on-bead either with sequencing-grade trypsin (1:50) (Promega, Walldorf, Germany) for 4 h at 800 rpm and 42°C or thermolysine (1:50 ...