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Citations for Promega :
451 - 500 of 1554 citations for Mayaro Virus E2 Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... proteins were digested with trypsin (Promega, Fitchburg, WI) for 14 hours at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko [46] ...
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bioRxiv - Neuroscience 2020Quote: ... Protein A/G magnetic beads (100 µL) (Promega) were washed with HB prior to addition to the RiboTag-IP fraction and were rotated at 4°C overnight ...
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bioRxiv - Immunology 2020Quote: ... protein samples were treated with PNGase F (Promega) according to manufacturer’s instruction under denaturing conditions ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested with trypsin (Promega, Madison, USA) at an enzyme-to-protein ratio of 1:100 (w/w ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were digested with sequencing-grade trypsin (Promega) at room temperature while shaking overnight ...
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bioRxiv - Neuroscience 2019Quote: ... proteins were digested with 0.3 µg LysC (Promega) for 16 h at 37°C followed by a second digestion step with 0.15 µg trypsin (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were digested with 1 µg trypsin (Promega) and subjected to liquid chromatography (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Ingel protein digestion using trypsin (Promega, Mannheim, Germany) was performed essentially as described before62 ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... AccuMAP™ Low pH Protein Digestion Kit (Promega) according to the manufactures protocol ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were deglycosylated by Endo-H (Promega) followed by PNGaseF (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with trypsin (sequencing grade, Promega) at 37°C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko (Shevchenko et al. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Proteins were then digested with modified trypsin (Promega) at an enzyme/substrate ratio of 1:50 in 100 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2023Quote: ... precipitated proteins were first treated with trypsin (Promega) in 50 mM ammonium bicarbonate solution ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko78 ...
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bioRxiv - Cell Biology 2023Quote: ... Add 200 μl of Protein Precipitation Solution (Promega) and mix by pipetting ...
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bioRxiv - Physiology 2023Quote: ... Proteins were digested using sequencing grade trypsin (Promega) at a 1:40 (w/w ...
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bioRxiv - Neuroscience 2023Quote: ... Purified HaloTag protein (#G4491) was purchased from Promega. Pluronic F-127 (# P3000MP) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Proteins were digested with Lys-C/Trypsin (Promega) at 37 °C overnight with rotation ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were digested with trypsin (Promega, Wisconsin, USA) in a ratio of 1:100 at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were on-bead digested with trypsin (Promega) at 37 °C for 16 h with orbital shaking ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were digested overnight with trypsin (Promega) at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Protein precipitations were digested with Trypsin (Promega, #V5113) diluted in Digestion buffer (100 mM Ammonium Bicarbonate and 10% Acetonitrile ...
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bioRxiv - Plant Biology 2021Quote: ... The pSPUTK promoter allowed in vitro protein synthesis using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 20µg of chromatin was subjected to buffer exchange through SP3 protein clean up protocol (87) and resuspended in AmBic-10mM DTT before proteins digestion with 1:50 trypsin (Promega V5280) for 18h ...
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bioRxiv - Systems Biology 2024Quote: ... and an aliquot of approximately 25 µg of protein was digested with Trypsin (Promega, Trypsin to protein ratio of 1:50) at 37°C overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... and human 293T cells as producer cells using the FUGENE HD transfection reagent (Promega), as described [50] ...
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bioRxiv - Pathology 2019Quote: ... human primary aortic SMCs were transfected with pGL4.34 Vector plasmids (E1350; Promega, Madison, WI) using Effectene Transfection Reagent (301425 ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... we used a 1/1500 artificial mix of promastigote DNA and human DNA (Promega) to reflect the median ratio found in clinical samples ...
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bioRxiv - Neuroscience 2022Quote: Open reading frames of human OR genes were subcloned into pCI (Promega, WI, USA) with a Rho-tag (the sequence encoding the first 20 amino acids of rhodopsin ...
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bioRxiv - Immunology 2023Quote: ... tailed with complete TRAC and TRBC1 gene segments amplified from human genomic DNA (Promega), respectively ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: SMC5/6 hexamer purified from E.coli with a C-terminal HALO tag on Nse2 was incubated with a 2-fold molar excess of Janelia-Fluor646 HaloTag Ligand (Promega; 12 µM Smc5/6 hexamer + 24 µM label). After incubation for 1 h at 25°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Each sample was then diluted 10-fold with 50 mM ammonium bicarbonate for digestion with 0.375 µg (for 15 µg protein samples) or 0.125 µg (for 5 µg protein samples) sequencing grade modified porcine trypsin (Promega, Madison, WI, USA). The samples were incubated at 45°C for 1 hour in a chilled microwave (CEM ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was diluted 1:1 with 50 mM ammonium bicarbonate and proteins were digested with trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were digested with trypsin (Promega Corporation, USA) at an enzyme-to-substrate ratio of 1:50 (w/w ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 μg of protein were digested with trypsin (Promega) in two steps (2 h at 37 ºC with 1:40 trypsin:protein ratio ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then digested with Trypsin Gold (Promega) 1:100 at 37 °C overnight in 10 mM ammonium bicarbonate pH 8.0 and the resultant peptides were cleaned up with POROS 20 R2 (Applied Biosystens) ...
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bioRxiv - Immunology 2019Quote: ... Protein material was digested with 600 ng Trypsin (Promega) overnight at 37°C at 300 rpm ...
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bioRxiv - Developmental Biology 2019Quote: ... Precipitated proteins were subjected to on-bead trypsin (Promega) digestion ...
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bioRxiv - Bioengineering 2021Quote: ... Proteins were digested with sequencing grade modified trypsin (Promega) at an enzyme to substrate ratio of 1:50 overnight at RT ...
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bioRxiv - Bioengineering 2020Quote: ... Proteins were digested with sequencing grade modified trypsin (Promega) at an enzyme to substrate ratio of 1:50 overnight at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were digested overnight with LysC/Trypsin mix (Promega) and sequencing-grade modified Trypsin (Promega ...
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bioRxiv - Neuroscience 2019Quote: ... Protein was digested with sequencing grade modified trypsin (Promega) using the FASP (Filter Aided Sample Preparation ...
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bioRxiv - Microbiology 2019Quote: ... proteins were predigested with 1.5 µg of rLysC (Promega) for 3 h at room temperature and samples diluted with 50 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2021Quote: ... with Broad Range Protein Molecular Weight Markers (V8491, Promega) used as molecular markers ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were harvested in passive lysis buffer 1x (Promega) at indicated times and
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bioRxiv - Plant Biology 2021Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nano LCMS/MS on a QExactive + mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo Fisher Scientific) ...