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Citations for Promega :
551 - 600 of 857 citations for M CSF Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: cDNA synthesis for gene expression analyses was performed using 500 ng RNA using Moloney murine leukemia virus (M-MLV) reverse transcriptase and random hexamer oligonucleotide (both from Promega, Southampton, UK) using 500 ng RNA in a 25 µl reaction ...
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bioRxiv - Microbiology 2019Quote: ... 2 μg of purified RNA for each sample was used to synthesize cDNA using M-MLV Reverse Transcriptase (Promega, Madison, WI, USA) at 42°C for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... or SB431542 (1µM) (ALK5 kinase inhibitor) and relative luciferase activities quantified after 16 hours using the Dual-Glo® Luciferase Assay System (Promega®). Histograms were generated and data analyzed by multiple paired t-tests and 2-way ANOVA tests using GraphPad (PRISM® 9.0.0).
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bioRxiv - Cell Biology 2022Quote: ... or combined to K02288 at 1µM (ALK1 kinase inhibitor and relative luciferase activities quantified after 16 hours using the Dual-Glo® Luciferase Assay System (Promega®). The TGFβ Reporter Assay was performed in TMLCs after transfection of the various PTPN14 constructs ...
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bioRxiv - Immunology 2022Quote: ... Samples were diluted to 2 M urea with 50 mM HEPES (pH 8.5) and proteolyzed with trypsin (Promega, 1:50 w/w) at room temperature overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... and diluted to 1 M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8 hr, Promega cat # V5113). After digestion ...
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bioRxiv - Cell Biology 2020Quote: ... Total 500 ng RNA was used to synthesize cDNA by using Moloney-murine leukemia virus (M-MLV) reverse transcriptase (Promega, Madison, WI). Diluted cDNA was used in each quantitative reverse transcription-PCR (qRT-PCR) ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized via reverse-transcription of 2-5 μg of RNA using M-MLV Reverse Transcriptase (200 U, Promega, Wisconsin, USA), random primers (10 ng/μl ...
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bioRxiv - Molecular Biology 2021Quote: ... Subsequent cDNA synthesis was done via reverse-transcription of 2µg of isolated RNA using M-MLV Reverse Transcriptase (Promega, Madison, WI, USA). The generated cDNA was used to analyze differences in gene expression by RT-qPCR employing iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2021Quote: ... A reverse transcription cocktail was subsequently prepared in the following manner (per reaction): 5µl of M-MLV 5X reverse transcriptase buffer (Promega, Catalogue no.-M531A), 5µl of 10 mM dNTP mix (Bioline ...
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bioRxiv - Cancer Biology 2022Quote: ... 1000 and 5000 µM) or remained untreated for 6 days and viability was analyzed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Dübendorf, Switzerland) to determine the adenosine triphosphate (ATP ...
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bioRxiv - Genetics 2022Quote: ... were transfected at 25 µM concentration in SK-N-SH cells at a density of 104 -105 cells using FuGene HD Transfection reagent (Promega Corporation, USA), per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The reaction was stopped by addition of 50 µl/well of 1 M HCl and absorbance read at 450 nm using a GloMax microplate reader (Promega, Dübendorf, Switzerland).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bound proteins were eluted with elution buffer 1 (50 mM Tris-HCl pH 7.5, 2 M urea, 5 ng/µL sequencing grade modified trypsin (Promega, Madison, WI, USA), 1 mM DTT ...
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bioRxiv - Biophysics 2021Quote: ... Approximately 3 μg of DNase I-treated RNA was reverse transcribed to cDNA using 10 μM of oligo (dT) primer and M-MLV reverse transcriptase (Promega, Madison, WI USA) in a 20 μL reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... The mixture was added with 25 mM NH4HCO3 to dilute urea to 1 M followed by overnight digestion with sequencing-grade trypsin (Promega, Madison, WI, USA) at an enzyme/protein ratio of 1:40 (wt/wt ...
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Evolution of sex hormone binding globulins reveals early gene duplication at the root of vertebratesbioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription (RT) was performed using 1 μg of RNA for each sample with M-MLV reverse transcriptase and random hexamers (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: One microgram of total RNAs from cells and tissues were reversely transcribed into cDNAs using M-MLV reverse transcriptase (Promega, Madison, WI, USA). Quantitative PCR analysis was performed by using SYBR Green SuperMix (Yeasen Biotech ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were then diluted to a final guanidine chloride concentration of 1 M with 100 mM ammonium bicarbonate and digested with sequencing grade porcine trypsin (Promega, 1:100 trypsin:protein) for 22 h at 37° C ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized using 1 µg of purified RNA and Promega M-MLV RNase H Minus Point Mutant Reverse Transcriptase (Promega, Madison, WI M3682) using the manufacturer’s recommended protocol for oligo dT-primed synthesis ...
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bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized using 1 µg of purified RNA and Promega M-MLV RNase H Minus Point Mutant Reverse Transcriptase (Promega, Madison, WI M3682) using the manufacturer’s protocol for oligo dT-primed synthesis ...
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bioRxiv - Plant Biology 2022Quote: ... They were later subjected to reverse transcription for 45 min at 37°C with 200 U of reverse transcriptase M-MLV (Promega, Madison, WI, USA) in the appropriate buffer.
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bioRxiv - Plant Biology 2023Quote: ... and 2 µg of RNA were used for reverse transcription into complementary DNA (cDNA) using M-MLV Reverse Transcriptase (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 μg of total RNA was reverse transcribed into cDNA with random hexamer and M-MLV Reverse Transcriptase H Minus (both from Promega, Madison, WI, USA) for real-time quantitative reverse transcriptase-PCR (qRT-PCR ...
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bioRxiv - Systems Biology 2024Quote: ... the samples were diluted with 50 mM HEPES (pH 8.5) to reduce urea concentration to 2 M and further digested overnight with trypsin (Promega, 1:50 w/w) at RT ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Neuroscience 2022Quote: ... anti-b-111-tubulin (mouse, Promega G712A, 1:500), Cleaved caspase-3 (rabbit ...
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bioRxiv - Molecular Biology 2019Quote: Goat anti-mouse IgG HRP 1:2000 (Promega W4021)
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bioRxiv - Developmental Biology 2022Quote: ... Anti–β-galactosidase monoclonal mouse antibody (Promega, 1:500) was co-incubated with anti-mCherry polyclonal rabbit antibody (Bio Vision ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... HRP-conjugated anti-mouse (Promega, catalog W4021, 1:6000), HRP-conjugated anti-goat (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... US) and TubβIII (mouse, 1:1000, G712A, Promega, UK). Slides were then incubated with the appropriate fluorophore-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... The HRP-conjugated goat anti-mouse (1:10,000; Promega) and goat anti-rabbit IgG (1:10,000 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody (Promega) (1:10,000 ...
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bioRxiv - Genomics 2022Quote: ... or anti-Mouse IgG HRP Conjugate (Promega, 1:2,000) diluted in blocking buffer followed by washing in TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary HRP-conjugated antibodies were purchased from Promega or GE Healthcare (anti-mouse Cat. No. W4021, Promega; anti-rabbit Cat ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-beta Galactosidase (Promega, Cat #Z378A. 1:1000). DAPI (1:1000 ...
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bioRxiv - Biochemistry 2019Quote: ... mouse monoclonal anti HALO IgG (catalog number G921A, Promega), mouse monoclonal anti GFP IgG (catalog number 11814460001 ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-β-Gal (1:1000, Promega, Cat#: Z3781), rabbit anti-β-galactosidase (1:5000 ...
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bioRxiv - Plant Biology 2019Quote: ... and anti-mouse HRP conjugate (W4021, Promega, Southampton, UK) antibodies.
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-β-galactosidase (1:1,000, Promega, Cat#: Z3781), rabbit anti-β-galactosidase (1:5,000 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse Anti-β-Galactosidase (#Z3781, 1:200) from Promega; rabbit anti-SWS (1:1000 from Doris Kretzschmar) ...
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bioRxiv - Molecular Biology 2023Quote: ... and anti- mouse IgG antibody (1:1,000, #S3721, Promega).
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bioRxiv - Neuroscience 2023Quote: ... and goat anti-mouse IgG (1:5,000; W4011, Promega) antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... and anti-mouse IgG alkaline phosphatase conjugated antibody (Promega). Band intensities were quantified by Image J ...
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bioRxiv - Developmental Biology 2023Quote: ... and mouse anti-β-galactosidase (Promega #Z378, 1:1000) or rabbit anti-mCherry (BioVision ...
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bioRxiv - Cell Biology 2023Quote: ... and Alkaline Phosphatase-conjugated goat anti-mouse IgG (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... HRP conjugated secondary antibodies anti-mouse (Promega, PR-W4012) and anti-rabbit (Invitrogen 65-6120) ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies (Anti-Rabbit/Mouse IgG HRP Conjugate, Promega) were used at 1:5000 in 5% Blotting-Grade Blocker (BioRad).
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bioRxiv - Developmental Biology 2024Quote: ... and secondary antibodies (anti-mouse HRP conjugate (Promega, W4028) and anti-rabbit HRP conjugate (Promega ...