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Citations for Promega :
1 - 50 of 2562 citations for Leptospira Biflexa Antigen Strain Patoc 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The following primary antibodies against the antigens were used: Tuj-1 (1:300, Promega), Synaptophysin (1:400 ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli JM109 strain (Promega) was transformed with the violacein pathway plasmid (pTU2S(VioAE)-b-VioBCD ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain KRX (Promega) and cultured in LB medium containing 100 µg/ml ampicillin ...
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bioRxiv - Microbiology 2020Quote: ... coli strains JM109 (Promega) and S17-1λpir/pLFX (E ...
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bioRxiv - Microbiology 2019Quote: Escherichia coli strain JM109 (Promega) was used for cloning and amplification of plasmids ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli strain BL21 (DE3) pLysS (Promega). A single colony was picked to inoculate a 5 mL starting culture that was allowed to grow overnight at 37 °C with 225 rpm before being diluted into large cultures (1 liter for PhoCl green state and red state ...
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bioRxiv - Microbiology 2024Quote: ... coli strain JM109 (Promega; Madison, WI). JM109 harboring pJBA132 was routinely cultured in Miller’s Luria-Bertani (LB ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated antigens were immobilized using streptavidin-coated magnetic beads (Promega). In each round ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... HaloTag-GST was used as a negative control antigen (Promega).
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bioRxiv - Microbiology 2020Quote: ... Transformations of the cloning strains DH5α (Promega), NEB 5-alpha (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... coli strains harboring pNL1.1 (Promega, Madison, WI) (see primers in Table S4 ...
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bioRxiv - Biochemistry 2019Quote: ... in Escherichia coli strain BL21(DE3) (Promega) and purified via glutathione-affinity column chromatography ...
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bioRxiv - Molecular Biology 2020Quote: ... NOCT antibodies were then purified from the verified serum by ammonium sulfate fractionation and then antigen affinity purification using recombinantly expressed HaloTag-NOCT (1-431) immobilized on HaloLink resin (Promega). The serum was purified over the immobilized NOCT column before elution with 5 M NaI and 1 mM ammonium thiocyanate ...
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bioRxiv - Cancer Biology 2019Quote: CHO K-1 cells overexpressing hPD-L1 and the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1 aAPCs, Promega) and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE ...
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bioRxiv - Immunology 2024Quote: ... Purified EDIII antigens were biotinylated using HaloTag PEG biotin ligand (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... coli strain JM109 (DE3) was purchased from Promega. Liquid growth media and cultures were prepared in acid washed glassware or sterile plasticware to minimise metal contamination ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain JM109 (DE3) was purchased from Promega. Liquid growth media and cultures were prepared in acid washed glassware or sterile plasticware to minimise metal contamination ...
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bioRxiv - Cell Biology 2022Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... the antigen was detected with the enhanced chemiluminescence (ECL) detection system (Promega) as specified by the manufacturer with Azure Sapphire Biomolecular Imager (Azure Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... faecium Aus0004 WT strain were circularized using T4RNA ligase (Promega), reverse transcribed by M-MLV RT (Promega ...
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bioRxiv - Microbiology 2021Quote: ... coli strain JW5925-149 using Platinum Taq DNA polymerase (Promega) and primers PC3 and PC4 with specificity for the regions flanking sslE ...
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bioRxiv - Plant Biology 2020Quote: ... Antigen on the membrane was visualized with horseradish peroxidase-coupled anti-rabbit IgG (Promega) for VHA-a1 and VHA-a3 ...
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bioRxiv - Immunology 2021Quote: ... RBD and NTD antigens were site-specifically biotinylated using Halotag PEG biotin ligand (Promega), following the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... Antigen retrieval was performed by incubation with 20 μg/mL proteinase K (Promega, USA) for 20 minutes ...
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bioRxiv - Microbiology 2019Quote: ... coli strain JM109 single use competent cells were purchased from Promega and used for transformations in E ...
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bioRxiv - Cell Biology 2023Quote: The bacterial expression vectors were transformed into the KRX strain (Promega). GST-tagged protein expression was induced by 0.1% rhamnose ...
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bioRxiv - Bioengineering 2023Quote: ... selections were performed using biotinylated Fc fusion antigen captured on SA-coated magnetic beads (Promega). Prior to each selection ...
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bioRxiv - Microbiology 2019Quote: ATP levels in strains were determined by BacTitre-GloTM Assay Kit (Promega) as previous described ...
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bioRxiv - Microbiology 2020Quote: ... coli strains was prepared using the Wizard Genomic DNA Purification kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... pSY5M-Pak4cat were introduced into Escherichia coli strain BL21(DE3) pLysS (Promega), and the fusion proteins were induced by incubation with 0.5 mM isopropyl-b-D-thiogalactoside (IPTG ...
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bioRxiv - Microbiology 2023Quote: ... coli strain S17-1λpir and confirmed by colony PCR (GoTaq polymerase, Promega) and Sanger sequencing (Microsynth ...
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bioRxiv - Biochemistry 2022Quote: Constructs of nanobody variants were transformed into Single step (KRX) E.coli strain (Promega) at end of molecular cloning ...
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bioRxiv - Microbiology 2023Quote: ... lactis strains were extracted using a “Wizard Genomic DNA Purification” kit (Promega, Madison, USA). Real-time PCR was done using a Rotor-Gene Q machine (Qiagen ...
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bioRxiv - Microbiology 2024Quote: DNA of strain JS3066 was extracted with a Wizard Genomic Purification Kit (Promega, USA). The genome sequencing and assembly was done by the BGI Medical Examination Co. ...
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bioRxiv - Molecular Biology 2024Quote: ... Approximately 25% confluent wells were transfected with 2 µg plasmid vector expressing SV40 antigen using Fugene Transfection Reagent (Promega) as described by manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... coli LPS strain W3110 (kindly provided by Robert Ernst) using FuGENE HD transfection reagent (Promega) for 6 hours.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli strains were performed using the PureYield™ Plasmid Miniprep System (Promega Corp., Madison, WI) according to manufacturer protocols ...
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bioRxiv - Microbiology 2020Quote: ... incubating and chromogenic reaction generating were similar to the ACE2 assay instead of that primary antibody binding to antigen was not required and that HRP-conjugated goat anti-human IgG antibody (Promega) would be used as the secondary antibody to detect binding of CB6 antibody to the antigens.
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bioRxiv - Bioengineering 2020Quote: Phage selections were done according to previously established protocols.30 Selections were performed using biotinylated antigens captured with streptavidin-coated magnetic beads (Promega). In each round ...
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bioRxiv - Biochemistry 2019Quote: Wild-type and mutant AP2ΔµC complexes were expressed in BL21 (DE3) pLysS strain (Promega, Madison, WI) by induction at 23 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from fresh cultures of these 18 strains on an automated platform (Promega Maxwell) as previously described [23] ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from AR16 and mutant strains using Wizard Genomic DNA Purification Kit (Promega) and transformed into B ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... gDNA was extracted from pooled DCR cultures from the two strain backgrounds using (Promega gDNA extraction kit) and stored at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... blaTEM and blaOXA were cloned from strain 48 into the cloning vector pGEM-T easy (Promega, UK) using the HiFi assembly kit (New England ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA (gDNA) was extracted from overnight cultures of selected strains using Genomic DNA purification Kit (Promega) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... SEA (0.2 mg/ml) and soluble schistosomula antigens (0.2 mg/ml) was determined using the Caspase-Glo® 3/7 Assay System (Promega, Sydney, Australia) according to the manufacturers’ instructions.
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bioRxiv - Microbiology 2020Quote: Genomic DNA from Xf strain CFBP 8070 was extracted with the Wizard genomic DNA Purification Kit (Promega, France) and used to prepare a 10-fold serial dilutions from 220 ng.mL−1 (corresponding to 0.8×108 copies.mL−1 of genomic DNA ...