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Citations for Promega :
501 - 550 of 620 citations for L lactate dehydrogenase B chain LDH B Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Transient transfection of A36-p14 hybrid constructs and the pE/L-LifeAct-iRFP670 expression in Vaccinia-infected cells was done using FUGENE (Promega) as described by the manufacturer ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μL of lysate was diluted with 30 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
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bioRxiv - Microbiology 2020Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalized to a mock-treated control to generate a fold induction.
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bioRxiv - Microbiology 2019Quote: ... injecting 50 µl per well of coelenterazine substrate (Nanolight Technologies, 2 µg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalised to a mock-treated control to generate a fold induction.
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bioRxiv - Molecular Biology 2021Quote: ... RNA pellets were resuspended in 8µl of DEPC water and incubated with µl (1U DNAse) and 1 µl of 10X DNAse buffer (Ref. M6101, Promega) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: The medium in 96-well plate was replaced at the indicated time points with 100 μl of PBS supplemented with 0.2 g/l EDTA and 50 μM FITC-VAD-fmk (Promega, USA) or 50 μM 1,1’,3,3,3’,3’-hexamethylindodicarbo-cyanine iodide (DiIC1(5) ...
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bioRxiv - Genomics 2021Quote: ... Membranes were washed three times in TBS-T before incubating with secondary antibodies (HRP-anti Mouse-IgG (H+L) (Promega)) for 45 min in TBS-T and 2% milk ...
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bioRxiv - Cell Biology 2020Quote: ... HRP Conjugate (W4011, 1:5000), and anti-Mouse IgG (H+L), HRP Conjugate (W4021, 1:5000) were purchased from Promega.
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bioRxiv - Microbiology 2021Quote: ... 40 µL of medium from selected wells was replaced with Nano-Glo HiBiT extracellular buffer with LgBiT and furimazine substrate (Promega). Readings were taken after a 30 min room temperature incubation as described above.
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to a concentration of urea of 2 mol L-1 and digested overnight with the addition of 2 µl sequencing-grade trypsin (Promega). After digestion ...
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bioRxiv - Immunology 2020Quote: ... Total DNA from blood and urine (around 50 μL) was extracted using a Maxwell 16 automat with the Maxwell blood DNA and cell LEV DNA purification kits (Promega), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of culture medium (300 μl) were taken at indicated timepoints and mixed with 100 μL of 4X RealTime-Glo extracellular ATP assay reagent (Promega) reconstituted in RPMI 1640/B27(-Ins ...
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bioRxiv - Bioengineering 2022Quote: ... 10 μL of the medium at several time points was collected for gLuc expression assay using Renilla Luciferase Assay System (Promega) and a luminometer ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of the lysates were used for the quantification of Firefly and Renilla Luciferase activity using a Luminometer (GloMax, Promega).
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of the lysates were used for the quantification of Firefly and Renilla Luciferase activity using a Luminometer (GloMax, Promega). The ratio between Renilla and Firefly is calculated for each experimental condition.
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed twice with 1X TBS-TT (10X TBS-TT is 10X TBS buffer with 5 mL/L Tween 20 and 5 mL/L Triton X) and once with 1X TBS before incubation with the secondary antibody (IgG anti-rabbit HRP conjugate, Promega) 1:1,000 diluted in blocking solution for 1 hr ...
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bioRxiv - Microbiology 2023Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Fold inductions were calculated by normalising to a mock-treated control.
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bioRxiv - Neuroscience 2023Quote: ... 1µl lysate was diluted with 39 µl PBS before being mixed with 40 µl of Dual-Glo FLuc substrate (Promega). After 10 min incubation ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected the following day with 1,000 ng of either the control plasmid or the BFP-miR-L plasmid using Fugene HD (Promega #E2311) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Necrosis was quantified by measuring release of lactate dehydrogenase in 30 µl samples of the medium and apoptosis by determining cell-associated Caspase3/7 activity using the respective kits from Promega Corp. ...
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bioRxiv - Molecular Biology 2023Quote: ... The final 50 µL translation reaction included the 5 µL RNA mixture described above and components from the Rabbit Reticulocyte Lysate system (Promega): 35 µL of rabbit reticulocyte lysate ...
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bioRxiv - Biochemistry 2020Quote: ... Cellular debris was then pelleted by centrifugation and either 5 μl or 10 μl of the clarified supernatant used in dual luciferase assays following the manufacturer’s prescribed protocol (Promega, cat # E1910). Data are shown as the ratio of RLUC to FLUC activity averaged over six experiments.
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bioRxiv - Biochemistry 2021Quote: ... HCT116 cells were plated on poly-L-lysine coated cover slips and transfected with ER calcium sensor ER-GCaMP6-150 using FuGENE HD (Promega Corp.). Experiments were carried out 1-2 days after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μl) were mixed with the master mixes containing 1 μl primers (10 μM each) and reagents of GoTaq G2 (Promega #M7845) and the final volume was 25 μl ...
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bioRxiv - Synthetic Biology 2023Quote: ... Crude extract (10 μl) was mixed with 40 μl of LARII and FLuc activity was determined using a GloMax®-Multi Detection System (Promega) with a 2 s delay and a 10 s integration times ...
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bioRxiv - Molecular Biology 2023Quote: Predigestion of bound proteins was performed by addition of 200 ng / µl of a 1:1 LysC (FUJIFILM Wako Pure Chemical Corporation, 125-02543) trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) mix ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µL of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) reagent (#G3582; Promega, Madison, WI) was added and incubated at 37°C for 45 mins ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then resuspended in 30 μL of 8 M Urea and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071).
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bioRxiv - Biochemistry 2024Quote: ... when the temperature was dropped to 22°C and isopropyl β-d-1-thiogalactopyranoside (IPTG) and L-rhamnose (Promega, Madison, WI) was added at 0.2 mM and 0.1% (w/v ...
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bioRxiv - Cancer Biology 2019Quote: ... rat or cynomolgus ICOS as target cells were co-incubated with ADCC reporter cells expressing human FcγRIIIa (V158; Promega) at a 5:1 ratio ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Genomics 2022Quote: ADGRG6_3 and ADGRG_4 sequences were PCR amplified from human genomic DNA and cloned into the pGL4.23 plasmid (Promega, E8411). Human preadipocytes ...
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bioRxiv - Molecular Biology 2020Quote: ... The promoter area of human Rpl28 gene was cloned at HindIII/NcoI site of the pGL3-basic (Promega, E1751) to make pGL3-reporters ...
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bioRxiv - Genomics 2022Quote: Approximately 500-bp regions of DNA containing rs80282103 and rs11154336 were amplified from purified human genomic DNA (Promega, #G1521) by PCR using engineered restriction sites to allow directional cloning into the multiple cloning region of the pGL4.23[luc2/minP] luciferase reporter vector (Promega ...
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bioRxiv - Neuroscience 2021Quote: We constructed luciferase reporter plasmids by cloning an ∼900 bp region containing human 1b30 into the pGL4.24 vector (Promega) upstream of the minP ...
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bioRxiv - Biophysics 2022Quote: ... pHalo-SMARCA4 expresses human SMARCA4 with HaloTag fused to the N-terminus under a CMVd1 promoter (Promega ORF FHC12075). pHalo-MED26 expresses human MED26 fused with a HaloTag at the N-terminus and was a kind gift from Joan Conaway’s lab ...
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bioRxiv - Cancer Biology 2022Quote: ... a 3kb region of the promoter containing HIC1 binding sites was amplified by PCR from human genomic DNA and cloned into the pGl4.26-luc vector (Promega); primers are listed in supplementary Table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
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bioRxiv - Microbiology 2023Quote: ... and Sp-EVs towards human endothelial cells was accessed by the CellTiter-Blue® (CTB) Cell Viability Assay (Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... After 30 minutes of incubation at 37°C in 5% CO2 we added 50,000 ADCC-RL cells (Jurkat cell line expressing luciferase gene under the control of the NFAT response element and stably expressing human FcγRIIIa V158; Promega) in 50 µl RPMI 1640 medium with 6% low-IgG serum (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... We amplified the regions from C57Bl/6 tail snip DNA or from human male genomic DNA (Promega catalog # G1471) using FastPhusion 2x Master Mix (Thermo Fisher catalog # F548L) ...
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bioRxiv - Cell Biology 2023Quote: ... TTBK2 KO cell lines were seeded on glass coverslips (Matrigel-coated for hPSCs) and the next day transfected with 0.5μg TTBK1-HaloTag® human ORF in pFN21A (FHC12512, Promega) or pglap1-TTBK2 (“GFP-TTBK2” ...
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bioRxiv - Genomics 2023Quote: ... Reference allele enhancer sequences for hZRS and hs737 were obtained via PCR cloning from human genomic DNA (Promega, G304A). Primers used for each enhancer sequence are outlined in Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cell surface levels of HiBiT-tagged human S1PR1 were monitored using a Nano Glo HiBiT Extracellular Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: IDH1 and NNT promoter sequences were amplified from genomic DNA of human primary melanocytes by PCR and cloned into pGL4.12 (Promega). Mutagenesis of E-boxes was performed using a QuikChange Site-Directed Mutagenesis Kit (Stratagene) ...
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bioRxiv - Molecular Biology 2024Quote: ... and the equivalent region in the human (−207/+5) were cloned into pGL3-basic Luciferase reporter-vector (E1751, Promega) using the forward primer containing XhoI restriction site ...
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bioRxiv - Cell Biology 2021Quote: ... Bait proteins were eluted with 25µl of elution buffer (2M Urea, 0.67M thiourea in 50mM Ammonium bicarbonate pH 8.0) containing LysC/Trypsin (Promega, Madison, WI, cat# V5071) to a final concentration of 5µg/ml followed by two elution with 50µl of elution buffer without LysC/Trypsin ...
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bioRxiv - Molecular Biology 2020Quote: ... the reaction mix in a final volume of 83 µl has been prepared by adding 21 µl of the Amino Acid Mixture (for a final concentration of 0.13 mM) and 80 units of RNasin (Promega Ref. number N2511) to 60 µl of RRL ...
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bioRxiv - Physiology 2024Quote: RNA was isolated from islets from ∼15-week-old male mice (WT and Kcnk16 L114P (L/P)) using Maxwell® 16 LEV simplyRNA Purification Kits (Catalog no. AS1280, Promega, USA). RNA integrity was analyzed using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of supernatant was removed from all wells and replaced with 100 μl of Bright-Glo luciferase substrate reagent (Promega; catalog #E2610). After 2 minutes ...