Labshake search
Citations for Promega :
601 - 650 of 1206 citations for L Aspartic Acid N T Boc B Bz Ester 13C4 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... and the resulting PCR product (3918bp) was cloned into pGEM®-T Easy Vector (Promega). Subsequently ...
-
bioRxiv - Microbiology 2019Quote: ... with primers specified in Supplementary Table 1 and cloned into vector pGEM-T Easy (Promega). A number of resulting clones was Sanger-sequenced and analyzed using the BLAST tool at PlasmoDB (www.plasmodb.org) ...
-
bioRxiv - Physiology 2019Quote: ... a ligation reaction was performed between the pGEM®-T Easy plasmid (Promega Milan, Italy) and the amplicon purified ...
-
bioRxiv - Neuroscience 2019Quote: ... The purified PCR product was subcloned into the pGEM-T Easy vector (Promega, Madison, WI) for Sanger sequencing ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR fragments were gel extracted and cloned with the pGEM-T easy Vector system (Promega) and transfected into XL10Gold competent cells (Agilent) ...
-
bioRxiv - Biochemistry 2021Quote: ... The amplified decalin synthase (DS) genes were cloned into the pGEM T-easy vector (Promega) and verified by sequencing ...
-
bioRxiv - Biochemistry 2021Quote: ... Tandem repeats of the DNA fragment were inserted into the pGEM-T Easy vector (Promega). The plasmid was amplified in Escherichia coli cells and purified ...
-
bioRxiv - Microbiology 2020Quote: ... End-modified PCR products were ligated into pGEM-T Easy vector (Promega, Madison, WI, USA). The vector-insert construct was used to transform chemically competent DH5α cells or OneShot Top 10 E ...
-
bioRxiv - Microbiology 2020Quote: ... These DNA probes were cloned into high copy number pGEM®-T Easy vector (Promega) and the recombinant vectors were used in Fur titration assay (FURTA ...
-
bioRxiv - Microbiology 2020Quote: ... 18S rRNA gene amplicons were cloned using the pGEM®-T Easy Vector System (Promega) and sequenced by Sanger sequencing ...
-
bioRxiv - Molecular Biology 2020Quote: ... the PCR product was purified and cloned in pGem-T Easy Vector Systems (Promega Corporation) as per the vendor’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... Products of the predicted size of 1463 bp were ligated into pGEM-T Easy (Promega) after which α-Select Bronze Efficiency Competent Cells (Bioline ...
-
bioRxiv - Genetics 2020Quote: ... then cloned using the pGEM T-Easy kit and manufacturer’s protocol (Promega, Madison, WI, USA). pGEM vector containing the desired PCR product was transformed into competent JM109 E.coli cells (Promega ...
-
bioRxiv - Microbiology 2021Quote: ... and the PCR product from this reaction was cloned into pGEM T-Easy vector (Promega), transformed into competent DH5-alpha E ...
-
bioRxiv - Molecular Biology 2021Quote: ... The amplified PCR products were cloned into pGEM-T easy vector (Promega, Madison, WI, USA). Sequencing for recombinant clones (n = 6 ...
-
bioRxiv - Genetics 2022Quote: ... Purified PCR products were TA-cloned using the pGEM-T Easy Vector System (Promega, A1360), according to the manual instructions ...
-
bioRxiv - Microbiology 2022Quote: ... The purified PCR product was cloned into the pGEM-T vector (Promega, Fitchburg, WI, USA), and later into the pGAPzαA vector (Invitrogen ...
-
bioRxiv - Molecular Biology 2022Quote: ... and cloned individually in a TA-cloning vector called pGEM®-T Easy Vector (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... After several intermediate cloning steps using the pGEM-T Easy Vector System (Promega, Madison, USA), final forward primer were designed from the 5’UTR (DcCENH3B ...
-
bioRxiv - Plant Biology 2023Quote: ... 5’RACE products were gel-purified and cloned in pGem®-T Easy vector (Promega). Independent clones were randomly chosen and sequenced by Sanger sequencing method.
-
bioRxiv - Neuroscience 2022Quote: ... These candidate iPSC clones were further sub- cloned into pGEM®-T Easy Vectors (Promega) to determine the genetic modifications at the allelic level using the same primers for the Exon 1 and 4 targeted regions ...
-
bioRxiv - Molecular Biology 2023Quote: Plasmid-based donor repair templates for CRISPR were prepared in pGEM-T Easy vector (Promega). Homology arms (each around 800-1000 bp ...
-
bioRxiv - Molecular Biology 2023Quote: ... Gel-purified PCR fragments were cloned into the pGEM-T Easy vector (Promega, cat# A137A) and sequenced using the Sanger’s method.
-
bioRxiv - Genomics 2023Quote: ... A simple cloning vector called pGEM-T was used for cloning (Promega, Madison, WI, USA). After that ...
-
bioRxiv - Molecular Biology 2023Quote: ... pGEM®-T Easy Vector Systems and T4 DNA Ligase were from Promega (Madison, WI). Restriction enzymes and Q5® High-Fidelity DNA Polymerase were from New England Biolabs (Ipswich ...
-
bioRxiv - Developmental Biology 2023Quote: ... Each product was cloned into the pANT vector (Nippon Gene) or pGEM-T easy (Promega) and sequenced with M13F-pUC or M13R-pUC primers (Table S3).
-
bioRxiv - Genomics 2023Quote: ... The synthesized fragments were gelpurified and cloned into the pGEM-T Easy vector (A1360; Promega) as per the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2023Quote: ... The obtained products were gel purified and cloned into the pGEM-T Easy Vector (Promega). Individual clones were sequenced and analyzed.
-
bioRxiv - Genetics 2023Quote: ... ∼0.75 kb amplicons were cloned using the pGEM-T Easy vector system (Promega; Madison, WI) following manufacturer’s protocol and the plasmids were then transformed into TOP10 cells (Invitrogen ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... PCR products of nifH and rnpB were each cloned into the pGEM-T plasmid (Promega). cDNA concentrations in nanograms per microliter were measured by Qubit 2.0 fluorometer (invitogen) ...
-
bioRxiv - Immunology 2024Quote: The cytotoxicity of CAR T cells was evaluated using a luciferase assay system (Promega, USA) following the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2024Quote: ... PCR products from the CAPS analysis were cloned into the pGEM T-easy vector (Promega) and purified using ExoSAP-IT reagent (Affymetrix) ...
-
bioRxiv - Biochemistry 2020Quote: hnRNP L and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L and SETD2 were constructed by PCR (Phusion polymerase ...
-
bioRxiv - Immunology 2023Quote: ... 50 ☐l of Bright-Glo Luciferase reagent (Promega E2620) was added to each well ...
-
bioRxiv - Immunology 2023Quote: We performed a Cbl-b cellular thermal shift assay (CETSA) using NanoLuc split luciferase technology (Promega). HEK293T cells were plated in 6-well plates (4e5 cells/ml ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2mM Mg(OAc)2 and 10μM of the amino acid mixture (Promega). Reactions were incubated with 5μM of the indicated nsp1 protein for 30 min on ice ...
-
bioRxiv - Biochemistry 2021Quote: ... chymotrypsin and peptide-N-glycosidase F (PNGase F) were obtained from Promega (Madison, WI, USA). Centrifugal filters (0.5 mL ...
-
bioRxiv - Cell Biology 2019Quote: ... RT) and sequentially digested with Lys-C (Wako) (o/n at RT) and trypsin (Promega) (6 h at 37 °C) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Kanamycin-resistant colonies were selected and cultured o/n purified with Plasmid Miniprep system (Promega) and verified by sequencing (Microsynth ...
-
bioRxiv - Plant Biology 2019Quote: ... The PCR amplification product was cloned into a pGEM®-T-Easy vector (Promega, Madison, Wisconsin) and named pHaHB4.2 ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR products were cloned into the pGEM-T Easy vector according to the manufacturer’s instructions (Promega) and sequenced using predesigned primers against the T7 promoter (MWG Eurofins).
-
bioRxiv - Microbiology 2021Quote: ... and was reverse transcribed into cDNAs using oligo-d(T) and M-MLV reverse transcriptase (Promega). Real-time PCR was performed with the specific primers listed in Table 2 using the LightCycler 480 (Roche ...
-
bioRxiv - Microbiology 2021Quote: ... The calibration curves were generated using serial dilutions of pGEM-T Easy plasmid DNA (Promega, USA) carrying a single copy of the target gene fragment (qp1F/qp1R) ...
-
bioRxiv - Plant Biology 2022Quote: ... The two PCR-amplicons were fused by overlap extension PCR and cloned into pGEM-T (Promega) by TA cloning ...
-
bioRxiv - Cell Biology 2019Quote: ... The MultiSite Gateway cassette was then ligated into pGEM-T Easy Vector (Promega UK, Southampton, UK) and the insert sequence was confirmed by sequencing (Source BioScience ...
-
bioRxiv - Plant Biology 2019Quote: ... of the entire or partial coding sequences cloned in pENTR vector or pGEM T-easy (Promega) and digested with respective endonuclease enzymes ...
-
bioRxiv - Genomics 2019Quote: The PCR products were excised from agarose gels and ligated into pGEM-T vector plasmids (Promega) with T4 DNA ligase (Promega) ...
-
bioRxiv - Genomics 2020Quote: The mid-transcript PCR product was ligated to pGEM®-T Easy vector (Promega Corporation, USA), following the manufacturer’s instructions and transformed into E ...
-
bioRxiv - Genetics 2021Quote: ... The fragment was then cloned using T/A cloning in the pGEMT vector (Promega, ref#A1360). For the Rsp satDNA probe ...
-
bioRxiv - Genomics 2021Quote: ... The amplicons were gel-purified and cloned into pGEM®-T Easy Vectors (Promega, Cat. #: A1360) using standard TA cloning techniques ...