Labshake search
Citations for Promega :
1 - 50 of 414 citations for Interleukin 23 Mouse Sf9 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... followed by transfection into Sf9 cells using FuGENE HD (Promega) to produce baculoviruses for RBD expression ...
-
bioRxiv - Microbiology 2021Quote: ... followed by transfection into Sf9 cells using FuGENE HD (Promega) to produce baculoviruses for RBD expression ...
-
bioRxiv - Genetics 2022Quote: ... Bacmids were used to transfect SF9 cells using FuGene HD (Promega). Baculovirus was generated through three rounds of amplification in SF9 cells grown in Sf-900 III media (ThermoFisher) ...
-
bioRxiv - Biophysics 2020Quote: ... coli and subsequently transfected into Sf9 insect cells using FuGENE (Promega) reagent ...
-
bioRxiv - Molecular Biology 2022Quote: ... Purified bacmids were transfected into Sf9 cells (Spodoptera frugoperda) using FuGene (Promega) and baculoviruses amplified for two rounds before protein expression in Hi5 cells.
-
bioRxiv - Microbiology 2023Quote: ... 1×106 Sf9 insect cells were transfected with bacmid DNA using Fugene (Promega) and cultivated for three days ...
-
bioRxiv - Cell Biology 2023Quote: ... The bacmid was transfected into Sf9 cells using Fugene HD transfection reagent (Promega). The obtained viral stock was amplified and used for protein expression ...
-
bioRxiv - Neuroscience 2023Quote: ... and Sf9 cells were transfected with the bacmid using FuGENE HD (Promega, E2311). Then ...
-
bioRxiv - Cell Biology 2022Quote: ... Bacmids were transfected to Sf9 insect cells by lipofection (FuGENE 6 Transfection Reagent, Promega), to generate baculovirus ...
-
bioRxiv - Bioengineering 2020Quote: ... 23] MSC spheroids were collected in passive lysis buffer (Promega, Madison, WI), and DNA content was determined using the Quant-iT PicoGreen DNA Assay Kit (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
-
bioRxiv - Immunology 2021Quote: ... Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
-
bioRxiv - Microbiology 2022Quote: ... Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
-
bioRxiv - Immunology 2022Quote: ... Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
-
bioRxiv - Immunology 2023Quote: ... Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
-
bioRxiv - Biophysics 2023Quote: Spodoptera frugiperda Sf9 cells were then transfected with these bacmids using FuGene Transfection Reactant (Promega). Baculoviruses were amplified in Sf9 cells at 28 °C.
-
bioRxiv - Immunology 2023Quote: ... Baculoviruses were generated by transfecting purified bacmid DNAs into Sf9 cells using FuGENE HD (Promega), and subsequently used to infect suspension cultures of High Five cells (Life Technologies ...
-
bioRxiv - Biochemistry 2020Quote: ... bacmid DNA containing all four subunits was transfected into Sf9 cells using FuGENE® HD (Promega) per manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... To transform the bacmid into Sf9 cells the transfection reagent FuGene 6 (Promega Corporation, Madison, USA) was used according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... 2-4μg bacmid was transfected into Sf9 cells in 6 well plates using Insect-XPRESS (Promega E2311). Cells were incubated at 2°C for 5 days and bacmid YFP expression assessed by Leica DM IL LED Fluo microscope using a green fluorescent protein filter cube ...
-
bioRxiv - Biophysics 2023Quote: ... were used to reverse transcribe a region of 23S rRNA using AMV reverse transcriptase (Promega). The region of cDNA containing the MS2 tag was amplified via PCR using primer pairs MS2_H98_quant_F and MS2_H98_quant_R or MS2_H25_quant_F and MS2_H25_quant_R ...
-
bioRxiv - Neuroscience 2023Quote: ... ND7/23 cells were transfected with FuGENE® 6 Transfection Reagent (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... obtained by amplification in DH10BacY cells were used to transfect Sf9 insect cells using FuGene transfection reagent (Promega). About 7 days after transfection the V0 virus was harvested and used to produce a V1 virus stock ...
-
bioRxiv - Molecular Biology 2022Quote: ... Fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
-
bioRxiv - Molecular Biology 2023Quote: ... fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
-
bioRxiv - Biochemistry 2024Quote: Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (E1910, Promega corporation, Madison, WI), and subsequently used to infect suspension cultures of Sf9 cells (2 × 106 cells/ml ...
-
bioRxiv - Biochemistry 2020Quote: 2.5 μg of bacmid DNA per construct obtained from amplification in DH10BacY cells were used to transfect 1 Mil Sf9 cells/per construct using Fugene transfection reagent (FuGENE HD, Promega). Virus at P0 was harvested and used to produce a stock Virus P1 solution to further infect 1 lt culture of Sf9 cells at 0.8-1 Mil/ml in SF921 medium containing Pen/Strep ...
-
bioRxiv - Biochemistry 2020Quote: ... Bacmid DNA was prepared by isopropanol precipitation and transfected into Sf9 (Spodoptera frugiperda) insect cells using FuGENE HD transfection reagent (Promega). After two rounds of virus amplification in Sf9 cells ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Recombinant baculovirus was produced by transfecting recombinant bacmids (2-3 μg) into Sf9 cells (5 mL, density of 4×105 cells per mL) using FuGENE HD Transfection Reagent (Promega) and Opti-MEM Reduced Serum Media (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2021Quote: A total of 10 μg of bacmid DNA was transfected into 6 wells of 2×106 adherent Sf9 cells (at 5×105 cells/ml) using the transfection reagent Fugene HD (Promega). 42–72 h post transfection ...
-
bioRxiv - Biochemistry 2023Quote: ... 1.5 µg of the pAC8RedNK vector and 0.5 µg of linearized baculoviral DNA were concurrently transfected into 1×106 Sf9 cells using 6 µl of FuGene HD (Promega) in ESF 921 Insect Cell Culture medium (Expression Systems) ...
-
bioRxiv - Biophysics 2023Quote: ... Bacmids for the wild type and all Xl-HAS-1 mutants were purified from 3 white colonies and transfected into Sf9 cells at 1×106 cells/mL using the FuGene reagent (Promega). Cells were maintained in ESF921 medium (Expression Systems ...
-
bioRxiv - Microbiology 2024Quote: ... 4 μg of purified bacmid DNA were transfected into 1×106 Sf9 cells in a well of a 6-well plate using 6 μL of FuGENE HD transfection reagent (Promega). After 7 days of incubation at 27°C ...
-
bioRxiv - Biophysics 2023Quote: ... The initial recombinant baculovirus was generated by transfecting adherent Sf9 cells with the bacmid DNA using the FuGENE HD reagent (Promega, USA) and used to infect suspension cells for virus amplification ...
-
bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 spike protein (23) into HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). Spike sequences from the following global strains REF were used ...
-
bioRxiv - Developmental Biology 2022Quote: ... Tails were dissected from the injected embryos at 23-24 hpf and lysed with passive lysis buffer (E1941, Promega) for subsequent Dual-Luciferase assay following the manufacturer’s instructions (E1910 ...
-
bioRxiv - Microbiology 2023Quote: The LDH levels in supernatant samples were measured using the CytoTox 96 Non-23 Radioactive Cytotoxicity Assay (Promega, G1780) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... Translation reactions were incubated for 23 min at 30 °C and nanoluciferase activity was monitored using the Nano-Glo Luciferase Assay Kit (Promega) in a Microplate Luminometer (Veritas) ...
-
bioRxiv - Systems Biology 2023Quote: ... Their identity was confirmed through cell line authentication carried out by BMR Genomics (Italy) by amplification of 23 STR loci (PowerPlex Fusion System kit, Promega). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Genomics 2022Quote: On- and off-target reporters were generated by sub-cloning a DNA fragment containing a fully complementary (23-mer) or partial siRNA target sites into the psiCHECK2 vector (Promega C8021) between Xho1 and Not1 restriction sites ...
-
bioRxiv - Cell Biology 2023Quote: ... reduced with 5 mM DTT and alkylated with 20 mM acrylamide.23 The reaction was quenched by addition of 5 mM DTT and 1.5 µg of trypsin (Promega, Madison, WI) was added to the sample ...
-
bioRxiv - Cell Biology 2020Quote: ... or mouse (W4021; Promega) IgGs ...
-
bioRxiv - Plant Biology 2023Quote: ... or anti-mouse (Promega) in 5% low-fat milk in TBS-T for 1 hour ...