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Citations for Promega :
1 - 50 of 257 citations for IL 18 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Lumit Human IL-1β Immunoassay (Promega) was performed according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: The coding sequence of the mature IL-18 was cloned into pET-20b plasmid (Promega) before being transduced into E ...
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bioRxiv - Microbiology 2021Quote: IL-18 truncations were generated through in vitro transcription/translation using pET-pro-IL-18 as a template in 10 µl reaction volumes following the manufacturer’s recommendations (TNT Coupled Reticulocyte Lysate; Promega) as previously [40] ...
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bioRxiv - Molecular Biology 2023Quote: ... and 18 μl of Fugene HD (Promega) to 300 μl of optiMEM ...
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bioRxiv - Immunology 2023Quote: ... pI.18-3xFlag-myRIG-I or pI.18-3xFlag-roRIG-I together with transfection control pLR-SV40-Renilla (Promega) and either of the reporter plasmids ISG54-Luc (69) ...
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bioRxiv - Biochemistry 2022Quote: ... with 18 ng of each construct using ViaFect (Promega), employing a transfection agent to plasmid ratio of 3:1 μL:μg ...
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bioRxiv - Immunology 2022Quote: ... and 18 μl of FuGENE 6 transfection reagent (Promega), according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... and 2.25 μg psPax2) and 18 μL of FuGENE 6 (Promega). The culture medium was changed 6-24 h later ...
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bioRxiv - Microbiology 2023Quote: ... prior to the addition of 18 µL of FuGENE (Promega #E2692). Pseudoviruses were also made by transfections using plasmids expressing the envelope of BG505.C233 or LAI (gift from Dr ...
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bioRxiv - Plant Biology 2023Quote: ... oligo(dT) 18 primers and the GoScript™ Reverse Transcriptase (Promega). qRT-PCR was performed using gene-specific primers (Supplementary Table 1 ...
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bioRxiv - Physiology 2024Quote: ... 18 µl of PCR master mix which includes MM (Promega #xxx), 1ul of Forward/Reverse primers (10mM) ...
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bioRxiv - Molecular Biology 2019Quote: ... or ∼20 µl of sample to 15-18 µl Fluc substrate (Promega).
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested at 37°C for 18 hr with trypsin (Promega) at a concentration of 1:25 (w/w ...
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bioRxiv - Neuroscience 2019Quote: ... at a ratio of 11:18:1 using FuGENE HD transfection reagent (Promega). Forty-two hours after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested for 18 h at 37°C with 0.5 μg trypsin (Promega). After digestion ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were digested for 18 hours at 37 °C with trypsin/LysC mix (Promega) at a protein-to-protease ratio of 25:1 ...
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bioRxiv - Systems Biology 2020Quote: ... Proteins were digested for 18-24 hours on beads with sequencing grade trypsin (Promega) in 50 mM HEPES buffer at 1:50 trypsin to protein ratio for frozen tumors and 2 μg trypsin per 10 μm section of FFPE ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Proteins were digested for 18 h at 37°C with 0.5 μg trypsin (Promega). After digestion ...
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bioRxiv - Immunology 2022Quote: ... After 18 h the neutralization assay was developed with a luciferase assay system (Promega), and the relative light units (RLU ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested for 18 h at 37°C with 0.5 μg trypsin (Promega). After digestion ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested for 18 h with trypsin/Lys-C Mix (Promega Corporation, USA) and 10 μg of each sample was used for proteome analysis ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Oligo dT(18)-primed reverse transcription was carried out with ImpromII Reverse Transcriptase (Promega). Semi-quantitative RT-PCR was carried out in the presence of [α-32P] dCTP (Perkin Elmer ...
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bioRxiv - Biochemistry 2019Quote: ... Digestion was performed for ∼18 hours at 37 °C using sequencing grade Glu-C (Promega). Peptides were analysed by nanoHPLC-MS/MS over a 65 min acquisition with elution from a 50 cm C18 PepMap column onto an Orbitrap Fusion Tribrid mass spectrometer via an Easy-Nano ionisation source ...
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bioRxiv - Microbiology 2020Quote: ... 18S rRNA gene amplicons were cloned using the pGEM®-T Easy Vector System (Promega) and sequenced by Sanger sequencing ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was reverse-transcribed to cDNA using oligo(dT)18 primer and M-MLV (Promega). qPCR was performed using SYBR Green (Bio-Rad ...
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bioRxiv - Genetics 2019Quote: Luciferase assays were performed 18 hours post-transfection using the Dual-Luciferase Assay System (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and digestion for 18 hours with 1 µg of TPCK- trypsin (Promega, Madison, WI, USA) in presence of 10% acetonitrile (ACN) ...
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bioRxiv - Neuroscience 2023Quote: ... plus helper plasmids pVSV-G (18 mg) and psPAX2 (27 mg) using FuGENE HD (Promega). DNA was incubated with 210 ml FuGENE HD in 4.5 ml Opti-MEM (Life Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 to 24 hours on bead with sequencing grade trypsin (Promega) in 50 mM HEPES buffer at 1:50 trypsin to protein ratio ...
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bioRxiv - Bioengineering 2020Quote: ... Unstimulated media (+CTRL) contained complete α-MEM medium only and stimulated media (+IL-1β) contained 20 ng/mL IL-1β (FHC05510, Promega, Madison, WI, USA) in complete α-MEM medium ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from fresh cultures of these 18 strains on an automated platform (Promega Maxwell) as previously described [23] ...
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bioRxiv - Cell Biology 2024Quote: ... Half of the sample was digested with 10 µg trypsin (Promega, 18 h at 37 °C). DOC was precipitated with 10 µL concentrated formic acid (conc ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were diluted with 18 μl 50mM HEPES pH 8.5 containing 0.2 μg modified porcine trypsin (Promega) and cleaved overnight at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Human Genomic DNA (G1521, Promega) was used as positive control and synthetic DNA was used to validate the LAMP assay performance ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells incubated for 18 h and harvested for cell viability quantitation using Cell-Titer-Glo (Promega; 20 µL) on a Synergy Neo plate reader (Biotek).
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bioRxiv - Microbiology 2020Quote: ... 500 ng human genomic DNA (Promega) or 5 μl of untreated or RNase-treated nasal swab nucleic acid isolates ...
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bioRxiv - Cancer Biology 2020Quote: ... Pooled human male genomic DNA (Promega) was randomly sheared (500-800bp ...
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bioRxiv - Systems Biology 2021Quote: ... a human cDNA pool (Promega Corporation), or obtained as synthesized double-stranded DNA fragments (gBlocks ...
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bioRxiv - Genomics 2021Quote: ... 50ng human genomic DNA (Promega, G304A), 2µM assembled T7-Tn5 transposase or Tn5 transposase was incubated at 55 °C for 7minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... human Female genomic DNA (Promega #G1521), as a reference ...
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bioRxiv - Biochemistry 2022Quote: HeLa and HEK293T cells were seeded on 18 mm glass coverslips at a density of 3×105 cells/mL and were transfected with FuGENE 6 (Promega) transfection reagent 24 h later ...
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bioRxiv - Physiology 2019Quote: ... The concentrated urea was diluted 12 times with 50 mM ammonium bicarbonate buffer and proteins were digested for 18 hours at 36°C using trypsin/LysC mixture (Promega) in 1:50 (w/w ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed using 2μg RNA of each sample and oligo (dT)18 primers by M-MLV reverse transcriptase (Promega, 28025013). Three independent biological samples were collected and analyzed ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram of RNA was reverse transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, https://worldwide.promega.com/). Quantitative real time PCR (qPCR ...
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bioRxiv - Biochemistry 2022Quote: ... Infected cells were harvested at 18 hpi and luciferase activity was measured using a luminometer-Glomax 20/20 (Promega, USA).
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bioRxiv - Microbiology 2022Quote: ... Infected cells were incubated for approximately 18-20 hours and luciferase was measured using the Promega BrightGlo luciferase kit following manufacturers’ instructions (Promega). Entry signal was normalized to the average signal for the “no spike” control ...
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bioRxiv - Microbiology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells7 for 18 hours at 37 °C before adding ONE-Glo reagent (Promega) for measurement of the luciferase signal by relative luminescence units (RLUs) ...