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Citations for Promega :
1 - 50 of 2526 citations for IL 1 beta Rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Beta III Tubulin (1:2000, Promega), and PSD-95 (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-Beta Galactosidase (1:1000, Promega), mouse anti-Beta Galactosidase (1:10 ...
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bioRxiv - Neuroscience 2021Quote: ... LacZ (Promega, Mouse anti-beta Galactosidase, 1:2000) and Nestin (Aves lab ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-beta galactosidase (Promega Z378825580610; 1:500), guinea pig anti-brk (provided by from Gines Morata ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-beta Galactosidase (Promega, Cat #Z378A. 1:1000). DAPI (1:1000 ...
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bioRxiv - Genetics 2021Quote: ... the following antibodies were used: mouse anti-beta-galactosidase (Promega; 1:100), mouse anti-Mmp1 (1:1:1 mixture of DSHB 3A6B4 ...
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bioRxiv - Genetics 2021Quote: Senescence was measured using 2 assays: 1/ Beta-Glo Assay kit (Promega # E4720), according to the manufacturer’s instructions and utilizing a luciferin-galactoside substrate (6-O-β galactopyranosylluciferin) ...
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bioRxiv - Genetics 2020Quote: Primary antibodies against beta-gal (Promega), LexA (Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... 50uL of Beta-Glo reagent (Promega, Madison, WI) and 100 uL of liquid culture was added to each well in a 96-well microplate and measured for beta galactosidase activity using the Synergy MX (BioTek ...
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bioRxiv - Developmental Biology 2023Quote: β-galactosidase activity was measured using Beta-Glo (Promega) essentially as described by Hook et al. ...
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bioRxiv - Bioengineering 2020Quote: Cell viability was calculated with beta-Glo assay (Promega)
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bioRxiv - Bioengineering 2021Quote: ... beta-mercaptoethanol (final concentration of 0.1 mM, Promega, USA) and Pen (1% ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with Anti-Beta-Galactosidase Purified Monoclonal Antibody (Promega), 1:200 in blocking solution for 2 h ...
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bioRxiv - Immunology 2023Quote: ... Lumit Human IL-1β Immunoassay (Promega) was performed according to manufacturer instructions ...
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bioRxiv - Bioengineering 2021Quote: ... β-galactosidase activity was measured using a Beta-Glo Assay System (E4720, Promega). The Npas2-LacZ expression data were uploaded to an online data analysis tool (CDD Vault ...
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bioRxiv - Cell Biology 2023Quote: ... the beta-galactosidase enzyme activity was measured using a kit (#E2000, Promega, USA). Luciferase readings were divided with beta-galactosidase readings to normalize for transfection differences ...
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bioRxiv - Developmental Biology 2019Quote: ... LacZ reporter activity was measured using the Beta-Glo® Assay system (Promega #E4720), following commercial protocols and yeast-specific methods (HOOK et al ...
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bioRxiv - Immunology 2021Quote: ... culture supernatant was removed from each well and Beta-Glo® reagent (Promega, Madison, WI) was added and the chemiluminescence activity (Relative Light Units-RLU ...
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bioRxiv - Cell Biology 2023Quote: ... Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...
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bioRxiv - Microbiology 2020Quote: ... At the indicated time points growth media was removed and 10 μl Beta-Glo (Promega, Madison, Wisconsin) was added per well ...
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bioRxiv - Bioengineering 2020Quote: ... Unstimulated media (+CTRL) contained complete α-MEM medium only and stimulated media (+IL-1β) contained 20 ng/mL IL-1β (FHC05510, Promega, Madison, WI, USA) in complete α-MEM medium ...
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bioRxiv - Developmental Biology 2024Quote: ... rat anti-Fat (kind gift from Helen McNeill) was used at 1:500 and mouse anti-β-galactosidase (Z3781, Promega; RRID:AB_430877) was used at 1:500 ...
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bioRxiv - Plant Biology 2023Quote: ... The selected interactions were verified by quantitative β-galactosidase assays using Beta-Glo Assay System (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell lysates were harvested the day after and luciferase and β-galactosidase activities were measured using Luciferase Reporter and Beta-Glo Assay Systems (Promega) following the manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2021Quote: ... or experimental constructs along with the ptcΔ136-GL3 luciferase reporter construct and beta-galactosidase transfection control (pSV-β-galactosidase; Promega, E1081).
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bioRxiv - Microbiology 2022Quote: ... the media was replaced with complete MEM (without NH4Cl) and cells incubated an additional 48 hours Transduced (LacZ+) cells were quantified using the Beta-Glo Assay System (Promega, Madison, Wisconsin) following the manufacturers’ protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... LacZ reporter activity was assayed in defined media (SD) supplemented with -Leu-Trp using the Beta-Glow® Assay System following commercially available protocols (Promega #E4720). In short ...
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bioRxiv - Developmental Biology 2019Quote: ... We measured LacZ reporter activity using the Beta-Glo® Assay System following the commercially available protocols and the yeast literature (Promega, Madison, WI) (Hook ...
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bioRxiv - Cell Biology 2021Quote: ... confirmed by positive beta-galactosidase staining and decreased proliferation rate (31) were transfected with peflin siRNA using Transfast (Promega Corp., Madison WI, USA) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The construct expresses rat GR fused with HaloTag protein (Promega, Madison, WI, USA) in the C-terminal domain under the CMVd1 promoter ...
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bioRxiv - Immunology 2023Quote: Activation of chemokine receptors was detected by measuring ß-galactosidase activity using the commercially available Beta-Glo assay system (Promega Corp, Madison, WI, USA) and luminescence signal was recorded on a multimodal plate reader (Enspire 2300 ...
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bioRxiv - Cancer Biology 2021Quote: The coding sequence of the mature IL-18 was cloned into pET-20b plasmid (Promega) before being transduced into E ...
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bioRxiv - Cell Biology 2019Quote: ... Cell signalling) or rabbit anti-TgTOM40 (1:2000, (van Dooren et al., 2016)) antibodies coupled to secondary horseradish peroxidase (HRP)(Promega for mouse and rabbit, Abcam for rat) conjugated antibodies (1:10,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Rat Gβ1 was cloned with a C-terminal SmBiT34 (peptide 86 or HiBiT, Promega) connected with a 15AA polypeptide linker ...
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bioRxiv - Biophysics 2022Quote: The pHaloTag-GR plasmid expresses rat GR fused to HaloTag (Promega, Madison, WI, USA) in the C-terminus regulated by a CMVd1 promoter and has been described previously (57) ...
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bioRxiv - Microbiology 2020Quote: ... Rabbit and rat primary antibodies were detected with horseradish peroxidase (HRP) conjugated anti-rabbit (Promega) and anti-rat (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2020Quote: ... rat C/EBP□-LIP or -LAP containing pcDNA3 or pSV2Zeo vector by using FugeneHD (Promega) according to the manufactures protocol ...
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bioRxiv - Genomics 2023Quote: ... The IL experiment RNA isolation was conducted using the Maxwell® 16 LEV plant RNA kit (Promega) (35) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were either treated with 50ng/ml rat Nerve Growth Factor (NG; Promega, Madison, WI, USA) at 0 ...
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bioRxiv - Immunology 2021Quote: ... IL-12 and TNFα were analyzed by semiquantitative PCR (SYBR Green assay Go Taq qPCR Master Mix; Promega) with specific primers (Metabion ...
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bioRxiv - Neuroscience 2019Quote: CHO-K1 cells (ATCC, cultured as described above) were transfected with rat TRAAK-GFP with FugeneHD (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: IL-18 truncations were generated through in vitro transcription/translation using pET-pro-IL-18 as a template in 10 µl reaction volumes following the manufacturer’s recommendations (TNT Coupled Reticulocyte Lysate; Promega) as previously [40] ...
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bioRxiv - Biochemistry 2022Quote: ... Rat Gβ1 with an N-terminal MHHHHHHSSGLVPRGSHMASHHHHHHHHHH-tag (His16) was fused with the SmBiT subunit (peptide 86, Promega)20 via a 15 amino acid (GSSGGGGSGGGGSSG ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then stimulated with 1 ng/mL of IL-1β for 6 h and subsequently washed with ice-cold PBS and lysed in Passive Lysis Buffer (PLB; Promega). Luciferase activity was measured in a Clariostar plate reader (BMG Biotech ...
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bioRxiv - Immunology 2023Quote: ... The supernatants were used to determine lactate dehydrogenase activity and IL-1β level using LDH-GloTM Cytotoxicity Assay (Promega, J2380) and Mouse IL-1 beta DuoSet ELISA (R&D Systems ...
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bioRxiv - Neuroscience 2019Quote: ... The U6 reporter construct was assembled by ligating a 663bp rat U6 sequence into the HindIII and NheI sites of the pGL4.23 vector (Promega) upsteam of the minimal promoter in this vector ...
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bioRxiv - Cancer Biology 2019Quote: ... rat or cynomolgus ICOS as target cells were co-incubated with ADCC reporter cells expressing human FcγRIIIa (V158; Promega) at a 5:1 ratio ...
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bioRxiv - Neuroscience 2021Quote: ... of miR-3594-5p was amplified from rat brain cDNA and cloned downstream of the Renilla luciferase reporter gene into the psiCHECK-2 vector (Promega).
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bioRxiv - Evolutionary Biology 2020Quote: ... and the orthologous 208 bp blind mole rat sequence (nanGal1 assembly; KL203377:255659-255866) into a luciferase pGL4.23[luc2/minP] vector (Promega, US). We cloned the insert in the same orientation with respect to the minP promoter and luciferase gene as the CNE and the Tdrd7 promoter are oriented in the genome ...
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bioRxiv - Biochemistry 2022Quote: ... The half site ARE reporter is derived from the minimal rat probasin sequence (Zhang et al., 2000) and was cloned into pGL3 via KpnI and NcoI restriction sites (Promega). pRL-TK (Promega ...