Labshake search
Citations for Promega :
401 - 450 of 1201 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... and Dounce homogenized in 500 µL of ice-cold RNase-free buffer polysome buffer (50 mM Tris pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% NP-40, 1 mM DTT, 200U/ml Promega RNasin ...
-
bioRxiv - Systems Biology 2023Quote: ... Digestion was performed overnight in 300 μL of 50 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer at pH 7.5 supplemented with endoproteinase Lys-C (Wako) and trypsin (Promega) at room temperature ...
-
bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:3 with 50 mM HEPES pH 8.5 and then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were diluted using one volume of dilution buffer (10 mM Tris pH 8, 0.4% NP40, 5 mM CaCl2, 2 U/mL RQ1 DNAse (Promega)) and then incubated with anti-Flag or anti-p400 antibodies coupled to agarose beads (Sigma ...
-
bioRxiv - Cancer Biology 2023Quote: ... beads were reconstituted in 5 μL 50 mM HEPES pH 8.0 buffer containing trypsin/rLys-C enzyme mix (Promega) at a 1:25 enzyme to protein ratio ...
-
bioRxiv - Cell Biology 2021Quote: ... LgBiT antibody (N710A) and HiBiT antibody were acquired from Promega. Chicken polyclonal MAP2 (ab5392) ...
-
bioRxiv - Molecular Biology 2021Quote: ... antibodies were detected using HRP-conjugated mouse secondary antibody (Promega). UAP56/DDX39B [1:2000] (custom generated51 ...
-
bioRxiv - Molecular Biology 2021Quote: ... antibody was detected using HRP-conjugated chicken secondary antibody (Promega).
-
bioRxiv - Molecular Biology 2021Quote: ... antibodies were detected using HRP-conjugated rabbit secondary antibody (Promega). Anti-Beta Tubulin III (Tuj1 ...
-
bioRxiv - Neuroscience 2021Quote: ... Dual luciferase assays were performed using the Promega™ Dual-Luciferase™ Reporter (DLR™) Assay Systems with the Promega™ GloMax™ 20/20 Luminometry System (Promega, Madision, WI). The rest of the assay was performed as previously described105.
-
bioRxiv - Plant Biology 2021Quote: ... We added 300μl of Proteinase K Buffer 2X (200 mM Tris-HCl pH7.5; 100 mM NaCl; 20 mM EDTA; RNasin PROMEGA 20 U/μl; 0.04 mg/ml Proteinase K) and incubated for 1 h at 55 °C ...
-
bioRxiv - Immunology 2023Quote: ... Secondary antibodies (Promega) were incubated for 1 h at RT ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were digested overnight at 37 °C with 5 μL of trypsin (1 μg dissolved in 50 mM HEPES pH 8.0, Promega V5111). The trypsin digestion was quenched by adding 4 μL of 1× EDTA-free protease inhibitor cocktail (Roche 11873580001) ...
-
bioRxiv - Neuroscience 2020Quote: ... 8 min, 4 °C) and resuspended in staining buffer (PBS, pH 7.4, with 0.5% RNase-free BSA (Promega, Heidelberg, Germany) and 0.2 U/μl of RNasin Plus RNase inhibitor (Promega)) ...
-
bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 6 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:25 (w/w ...
-
bioRxiv - Cell Biology 2022Quote: ... The bound proteins were digested by resuspending the beads in 200 μL freshly prepared digestion buffer (2M Urea, 100 mM Tris-HCl pH 8, 100 mM DTT) with 0.5 ug trypsin (Promega, V5111) and overnight incubation at room temperature while shaking ...
-
bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 4 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:250 (w/w ...
-
bioRxiv - Immunology 2019Quote: ... Samples were diluted 10-fold with 50 mM Triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega) added at a 1:50 enzyme:substrate ratio (wt/wt).
-
bioRxiv - Cell Biology 2019Quote: ... Samples were diluted 10-fold with 50 mM triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega) at a 1:50 enzyme:substrate ratio (wt/wt).
-
bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were prepared by addition of 0.3N hydrochloric acid then 450nM Tris (pH=8) and metabolites were analyzed using the Glutamine/Glutamate Glo Assay (Promega; J8021) per manufacturer’s instruction.
-
bioRxiv - Biochemistry 2021Quote: ... of 50mM Tris-HCl pH 8.5 containing LysC (Wako Chemicals) at 1:100 (w/w) ratio and trypsin (Promega V5113) at 1:100 (w/w ...
-
bioRxiv - Biochemistry 2022Quote: ... Purified AaEnc and BSA were individually buffer exchanged into TBS pH 7.5 and mixed in a 40:1:1 molar ratio with commercially purchased trypsin (Promega, USA) and chymotrypsin (Promega ...
-
bioRxiv - Developmental Biology 2023Quote: ... cells were washed twice in PBS pH 7.4 and lysed in 75 µl 1x Passive Lysis Buffer (Promega, Madison, USA). The Dual-Luciferase® Reporter Assay (Promega ...
-
bioRxiv - Biophysics 2023Quote: ... Samples were diluted 5-fold with 25 mM Tris pH 8.0 and 1 mM CaCl2 prior to digesting them with trypsin (Promega, V511X) at a 1:30 enzyme-to-protein ratio at 37 °C in a dry bath for 16 h.
-
bioRxiv - Microbiology 2023Quote: ... In solution digests were performed overnight at pH 8 and 37 °C with mass spectrometry grade trypsin (Promega, Madison, WI) at a 1:33 enzyme-to-protein ratio and quenched with formic acid (0.15%) ...
-
bioRxiv - Cancer Biology 2023Quote: ... pH 11 and the fluorescence was measured at 360 nm (ex) / 450 nm (em) using GloMax Explorer plate reader (Promega). The heparan sulfatase activity was confirmed by our recently described 6-O desulfation assay using GlcNS6S-GlcA-Glc6SNS-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP (2S2-6S4 ...
-
bioRxiv - Molecular Biology 2019Quote: ... or ∼20 µl of sample to 15-18 µl Fluc substrate (Promega).
-
bioRxiv - Microbiology 2020Quote: ... each reaction contained 20 μL of 100 ng of pGL3 plasmid (Promega), mixed with 100 ng Igf2 P3 Nluc (or empty vector or constructs obtained from Igf2 P3 Nluc) ...
-
bioRxiv - Cell Biology 2020Quote: ... and lysed in 20 μl of 1x passive lysis buffer (PLB) (Promega) for downstream analysis ...
-
bioRxiv - Cancer Biology 2022Quote: ... CellTiter 96 Aqueous One Solution MTS Reagent (20 μL, Promega Cat# G3582) was added to each well and incubated at 37°C for 1 h ...
-
bioRxiv - Microbiology 2020Quote: ... Amplicons (20) under each condition were subcloned in pGEM-T Easy (Promega) and sequenced (Multi-Disciplinary Laboratories Unit ...
-
bioRxiv - Cell Biology 2019Quote: ... and 20 ng/ml basic fibroblast growth factor (catalog no. G507A, Promega). Human primary myoblasts were passaged the same way as C2C12 cells ...
-
bioRxiv - Molecular Biology 2021Quote: ... 20 μl of 5 ng μl−1 trypsin (sequencing grade, Promega®) in 50 mM AHC plus 1 mM CaCl2 were added to each well and incubated for 20 min at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... Proteolysis reactions were performed with 20 ng α-chymotrypsin (sequencing grade, Promega) at 25°C and at 0 ...
-
bioRxiv - Cancer Biology 2021Quote: ... 20 µL of resazurin (CellTiter-Blue® Cell Viability Assay kit – Promega) were added to perform the viability assay ...
-
bioRxiv - Neuroscience 2021Quote: ... Heads (10/sample) were homogenized in 20 μL Glo Lysis buffer (Promega), incubated at room temperature for 15 minutes ...
-
bioRxiv - Cell Biology 2019Quote: ... all cells were given CellTiter Blue (20 μL/well, Promega, Cat#: G8081) and incubated for the remaining time of the experiment ...
-
bioRxiv - Biochemistry 2022Quote: ... 20 μL of MTS solution (CellTiter 96 Aqueous One solution reagent, Promega) was added to each well ...
-
bioRxiv - Plant Biology 2022Quote: ... and 10 units T4 DNA ligase (HC, 20 units μl−1, Promega). In a thermocycler ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 10 µl of a mix of LgBiT (1:20 dilution; #N2421, Promega) and furimazine (1:10 dilution ...
-
bioRxiv - Molecular Biology 2022Quote: ... 20 µl of Cell Titer Blue® reagent (Promega, Madison, WI, USA) was added to the cells and incubated for 4 h at 37 ºC ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by digestion in 20 μl of 10ng ul-1 Trypsin (Promega) in 25 mM NH4HCO3 (pH 8.0-8.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were placed in 20% Nano-Glo® Live Cell Reagent (Promega) mixed with 80% DMEM containing 1% FBS ...
-
bioRxiv - Genomics 2023Quote: ... 20 μl of Proteinase K (Promega Wizard HMW DNA Extraction Kit A2920) was added and the samples mixed by inverting 10 times and incubated 15 minutes at 56°C followed by chilling on ice for 1 minute ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 μl (20 ng/μl) of modified sequencing-grade trypsin (Promega, V5111) was spiked into the solutions and the samples were incubated at 37 °C overnight ...
-
bioRxiv - Genetics 2024Quote: ... in 2x SSCT (0.1% Tween-20 in 2x SSC (Promega, Cat# V4261)) for 5 min at RT ...
-
bioRxiv - Immunology 2024Quote: ... Add 20 µl/well of 1x luciferase cell culture lysis reagent (Promega). Add 100 µl of Luciferase Assay reagent (Promega ...
-
bioRxiv - Microbiology 2024Quote: ... supplemented with 1:20 of the extended live cell substrate EndurazineTM (Promega) was added to each well ...
-
bioRxiv - Microbiology 2020Quote: ... antibody with HRP-conjugated anti-mouse secondary antibody (GE Healthcare or Promega). In addition to the experimental samples ...
-
bioRxiv - Plant Biology 2020Quote: ... an immunoblot with anti-halo antibody (Anti-HaloTag® Monoclonal Antibody-Promega) was performed to confirm protein expression and binding efficiency for each TF ...