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Citations for Promega :
651 - 700 of 5848 citations for Human V Set And Immunoglobulin Domain Containing Protein 2 VSIG2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 80 µl of PNK buffer containing 4U Thermosensitive Alkaline Phosphatase (TSAP) (Promega) and 2µl RNasin® (Promega ...
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bioRxiv - Microbiology 2021Quote: ... dNTP nucleotide mix containing 200 µM concentrations of each nucleotide (Promega, USA), and 1.25U of GoTaq DNA polymerase (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... and pGL4.74 containing the Renilla luciferase reporter gene (0.5 μg/dish, Promega), as an internal control ...
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bioRxiv - Immunology 2020Quote: ... Washed beads were resuspended in 50mM ammonium bicarbonate containing 25ng trypsin (Promega) by brief bath sonication and incubated at 37°C O/N ...
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bioRxiv - Immunology 2020Quote: ... followed by a medium change to supplemented DMEM containing 1µM luciferin (Promega). Data were further processed and analyzed using Chronostar 3.0 [49].
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bioRxiv - Biophysics 2022Quote: ... and PiggyBac transposon plasmid containing puromycin selection using FuGENE (Promega, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... containing flanking sequences that matched the pNL3.2NF-κB-RE plasmid (N111A, Promega). The pNL3.2NF-κB-RE plasmid was PCR amplified with primers containing sequences flanking the 5’UTR ...
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bioRxiv - Neuroscience 2022Quote: ... followed by 25 μL of the HBSS containing GloSensor cAMP Reagent (Promega). Plates were kept in a dark place at room temperature for two hours to equilibrate cells with the reagent ...
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bioRxiv - Cell Biology 2023Quote: ... neurons were incubated in media containing 50nM JF646 HaloTag ligand97 (Promega GA1120) or JFX554 HaloTag ligand98 (Kind gift of L ...
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bioRxiv - Microbiology 2024Quote: ... which included a 5 µl nucleotide mix containing 2.5 mM NTPs (Promega). For each reaction ...
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bioRxiv - Cancer Biology 2021Quote: ... The RealTime Glo Annexin V Apoptosis and Annexin Assay was purchased from Promega (San Luis Obispo, CA, USA). Phospho-GSK-3β (Ser9) ...
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bioRxiv - Molecular Biology 2021Quote: Cell apoptosis and necrosis were measured by a RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay (Promega) (60) ...
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bioRxiv - Molecular Biology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) (Promega, Cat. No. G109C) cell viability assay was performed ...
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bioRxiv - Cell Biology 2022Quote: ... β-arrestin 2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin 2; Promega, plasmid no. CS1603B118) was chosen as it has previously been used successfully with other class B GPCRs ...
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bioRxiv - Plant Biology 2021Quote: ... The pSPUTK promoter allowed in vitro protein synthesis using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 20µg of chromatin was subjected to buffer exchange through SP3 protein clean up protocol (87) and resuspended in AmBic-10mM DTT before proteins digestion with 1:50 trypsin (Promega V5280) for 18h ...
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bioRxiv - Systems Biology 2024Quote: ... and an aliquot of approximately 25 µg of protein was digested with Trypsin (Promega, Trypsin to protein ratio of 1:50) at 37°C overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... and human 293T cells as producer cells using the FUGENE HD transfection reagent (Promega), as described [50] ...
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bioRxiv - Pathology 2019Quote: ... human primary aortic SMCs were transfected with pGL4.34 Vector plasmids (E1350; Promega, Madison, WI) using Effectene Transfection Reagent (301425 ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... we used a 1/1500 artificial mix of promastigote DNA and human DNA (Promega) to reflect the median ratio found in clinical samples ...
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bioRxiv - Neuroscience 2022Quote: Open reading frames of human OR genes were subcloned into pCI (Promega, WI, USA) with a Rho-tag (the sequence encoding the first 20 amino acids of rhodopsin ...
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bioRxiv - Immunology 2023Quote: ... tailed with complete TRAC and TRBC1 gene segments amplified from human genomic DNA (Promega), respectively ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µl ViaFect™ (Promega, Madison, WI) and 40 µl Gibco Opti-MEM reduced serum media (Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: The psiCHECK-2 vector (Promega, Cat #C8021) was used to construct plasmids for dual-luciferase reporter assay ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mg/ml proteinase K (Promega, v3021)) at 37 °C overnight followed by addition of 10 μl of 3M potassium acetate and incubation at room temperature for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µg of sequencing grade Trypsin (Promega) was added ...
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bioRxiv - Microbiology 2020Quote: ... and 2 μL RNasin RNase inhibitor (Promega). The transcription reaction was incubated overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... and 2 μg Sequencing Grade Trypsin (Promega). The beads were shaken overnight at 37 °C and pelleted by centrifugation (1,400 rcf ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µg of sequencing grade trypsin (Promega) was then added to the samples and they were digested overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: The psiCHECK-2 control vector (Promega, C8021) and the psiCHECK-RL-Ifnb1 3′ UTR vector were described before26 ...
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bioRxiv - Microbiology 2021Quote: ... 2 µL dNTP (Promega U151B, 2.5 mM), 1.5 µL MgCl2 (25 mM) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 0.05 μg LgBiT-β-arrestin-2 (Promega) plus 0.9 µg pcDNA3.1 for 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... 2 μL of luciferin reagent (Promega BrightGlo) was added and luciferase activity detected (Perkin Elmer Envision) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 or 4 (Promega Madison, WI, USA) (62) ...
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bioRxiv - Bioengineering 2022Quote: ... 2 U/µL RNasin Ribonuclease Inhibitor (Promega), 1.2 µM PrimeTime primers ...
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Optimization and deoptimization of codons in SARS-CoV-2 and the implications for vaccine developmentbioRxiv - Evolutionary Biology 2022Quote: Dual-luciferase assay (psiCHECK-2 vector, Promega) was selected to analyze the effects of the viral mutation on protein synthesis ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We then added 2 μg trypsin (Promega) in 100 mM TEABC and incubated overnight at 30 °C ...
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bioRxiv - Microbiology 2023Quote: ... and 2× GoTaq qPCR Master Mix (Promega). qPCR was performed using the MyGo Pro real-time PCR instrument (IT-IS Life Science Ltd.) ...
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bioRxiv - Molecular Biology 2024Quote: ... and digested with 2 µg Trypsin (Promega) overnight at 37 °C shaking at 1200 RPM on a thermomixer ...
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bioRxiv - Cancer Biology 2022Quote: ... Each sample was then diluted 10-fold with 50 mM ammonium bicarbonate for digestion with 0.375 µg (for 15 µg protein samples) or 0.125 µg (for 5 µg protein samples) sequencing grade modified porcine trypsin (Promega, Madison, WI, USA). The samples were incubated at 45°C for 1 hour in a chilled microwave (CEM ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was diluted 1:1 with 50 mM ammonium bicarbonate and proteins were digested with trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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bioRxiv - Immunology 2020Quote: ... The following day cells were transfected with plasmids encoding for FLAG-tagged NLRP1 (2 μg) or the indicated DPP9 construct (2 µg) with FuGENE HD according to manufacturer’s instructions (Promega). Cells were harvested ...
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bioRxiv - Plant Biology 2022Quote: ... The medium was aspirated and fresh medium containing 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, premixed in 5:1 ratio, Promega) was added to the treated cells ...
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bioRxiv - Molecular Biology 2023Quote: ... precipitated proteins were eluted by incubation of beads with Elution Buffer (2 M Urea, 50 mM Tris pH 7.5, 2 mM DTT, 20 µg/ml Trypsin Gold (Promega)) for 30 min at 37°C shaking at 1,400 rpm in the dark ...
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bioRxiv - Microbiology 2023Quote: ... 20 μL of MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) inner salt (Promega) was added to each well and the plate was further incubated for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 μL of [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (MTS) (Promega, #G3582) were added and incubated at 37°C for 3 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were digested with trypsin (Promega Corporation, USA) at an enzyme-to-substrate ratio of 1:50 (w/w ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 μg of protein were digested with trypsin (Promega) in two steps (2 h at 37 ºC with 1:40 trypsin:protein ratio ...