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Citations for Promega :
551 - 600 of 1480 citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... Transformants were miniprepped with the PureYield™ Plasmid Miniprep System (Promega A1223) and Sanger sequenced verified with GeneWiz ...
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bioRxiv - Microbiology 2021Quote: ... The product was cloned directly into the pGEM-T easy plasmid (Promega), and subcloned into the pRKlacZ290 vector ...
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bioRxiv - Microbiology 2020Quote: ... each reaction contained 20 μL of 100 ng of pGL3 plasmid (Promega), mixed with 100 ng Igf2 P3 Nluc (or empty vector or constructs obtained from Igf2 P3 Nluc) ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection of plasmids was done 24 hours after plating using FuGENE6 (Promega) at a DNA/FuGENE ratio of 1:4.5 according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... transfection was performed using 1.5μg plasmid together with 4.5μl FuGENE HD (Promega) and 100μl Opti-MEM per well according to the manufacturer’s guidance ...
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bioRxiv - Cancer Biology 2022Quote: ... sgRNA oligonucleotides were ligated to plasmids using T4 DNA ligase (Promega #M1801) and BbsI restriction enzymes (NEB #R0539L ...
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bioRxiv - Molecular Biology 2022Quote: ... The pGL3-basic plasmid containing a reporter gene was purchased from Promega Co ...
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bioRxiv - Molecular Biology 2020Quote: The plasmid for HaloTag (Halo) fusions was generated by cloning HaloTag (Promega) in the pBluescript SK (- ...
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bioRxiv - Neuroscience 2021Quote: ... the plasmid was re-ligated overnight at 16°C (ligase from Promega) and transform in Stbl4 bacteria ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3 ng of the Renilla Luciferase expressing pRL-SV40 plasmid (Promega) for normalization ...
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bioRxiv - Microbiology 2020Quote: ... All three DNA fragments were cloned into the pGEM-T plasmid (Promega) digested with SalI and NcoI.
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bioRxiv - Immunology 2022Quote: ... Cell transfection with plasmids was conducted using ViaFect™ Transfection Reagent (Promega) or Lipofectamine® 2000 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The DNA plasmid mix was added to 18µl of Fugene-6 (Promega) transfection reagent in 200µl of pre-warmed Opti-MEM (Gibco ...
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bioRxiv - Microbiology 2019Quote: The following plasmids were used in transfection experiments: HaloTag vector (Promega; pHTN), pHTN-NSP1 (SA11-4F) ...
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bioRxiv - Neuroscience 2019Quote: ... pDONR201 plasmid containing Ntrk2 was purified using a mini-prep kit (Promega) and recombined into pEF5/FRT/V5-DEST destination vector by LR clonase II reaction (ThermoScientific) ...
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bioRxiv - Bioengineering 2020Quote: ... and cloned the isolated DNA into a pGEM-TEasy plasmid vector (Promega) with standard cloning protocols ...
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bioRxiv - Synthetic Biology 2020Quote: ... Their plasmid DNA was isolated using a PureYield miniprep kit (Promega, USA) and was further analysed with restriction digestion using the enzymes EcoRI-HF ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells were transfected with plasmid DNA using Fugene 6 reagent (Promega) by following the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasmid DNAs were transfected into HEK293 cells with ViaFect Transfection Reagent (Promega) according to manufacturer’s instruction.
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bioRxiv - Synthetic Biology 2020Quote: ... either murine Il1rn or firefly luciferase from the pGL3 basic plasmid (Promega), and an NF-κB-negative regulatory element (NRE-5’-AATTCCTCTGA-3’ ...
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bioRxiv - Molecular Biology 2021Quote: ... 250 ng each of the pHMRluc (1) and pGL3-control plasmids (Promega) were added in addition to the indicated amounts of SP expression constructs.
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA was purified with a Wizard Plus SV Miniprep kit (Promega). Restriction enzyme digestions were performed according to the manufacturer’s instructions (Thermo Fisher Scientific or NEB) ...
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bioRxiv - Immunology 2021Quote: ... 4D6 cells were co-transfected with a renilla control plasmid (pRL Promega), and a luciferase reporter plasmid (pGL4.10[Luc2] ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.2 μg of pMD2.G envelope plasmid using FuGENE 6 (Promega). Cells were incubated up to 48 hrs and then lentivirus-containing media was collected and concentrated with Lenti-X concentrator (Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... 293T/17 and HeLa cells were transfected with plasmids using FugeneHD (Promega) or Lipofectamine3000 (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2019Quote: ... Assays were normalised by co-tranfected with renilla luciferase plasmid pGL4.74 (Promega) or by assaying total protein in extract ...
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bioRxiv - Bioengineering 2021Quote: ... All plasmids used for transfection were amplified using a Maxiprep kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... The pEYKBA plasmids were transfected into 293T cells using FuGENE HD (Promega) as described in section 2.2.3 ...
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bioRxiv - Microbiology 2021Quote: ... All plasmids used for transfection were amplified using a Maxiprep kit (Promega), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Each transfection consisted of 75 ng of luciferase reporter plasmid psiCHECK2 (Promega) or luciferase-5boxB plasmid psiCHECK2 −5boxB ...
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bioRxiv - Immunology 2021Quote: ... and HIV Rev plasmid using Fugene 6 transfection reagent (Promega, Madison, WI). Virus-containing medium was titrated to ensure undersaturating conditions for infection of H1299 cells ...
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bioRxiv - Biophysics 2022Quote: ... and PiggyBac transposon plasmid containing puromycin selection using FuGENE (Promega, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... containing flanking sequences that matched the pNL3.2NF-κB-RE plasmid (N111A, Promega). The pNL3.2NF-κB-RE plasmid was PCR amplified with primers containing sequences flanking the 5’UTR ...
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bioRxiv - Molecular Biology 2023Quote: ... the psPax2 envelope and the vsv-G packaging plasmids using Fugene6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... HIV-LTR plasmid constructs were transfected into Jurkat cells using ViaFect (Promega), 24 h post transfection cells were treated with GSK2981278 (40 μM ...
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bioRxiv - Cancer Biology 2023Quote: ... along with the plasmid of interest using Fugene 6 transfection reagent (Promega). All shRNA plasmids used were purchased from Sigma ...
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bioRxiv - Microbiology 2023Quote: The TRPPCa reporter plasmid p9X-NL1.2 was based on pNL1.2[NlucP] (Promega) that encoded Nanoluciferase fused to a C-terminal PEST sequence from mouse ornithine decarboxylase ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmid was transfected into HEK293T cells using FuGENE HD (Promega, E2312) and proteins were allowed to express for 20h ...
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bioRxiv - Microbiology 2023Quote: Nanoluciferase (Nluc) gene was obtained from the pNL1.1 plasmid (Promega Corporation, USA). The nanoluciferase encoding gene was fused to the C-terminus of either CFP-10 or Rv1636 following a short linker sequence (GGSGGGSSGG ...
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bioRxiv - Cell Biology 2023Quote: Plasmid DNA transfections were performed using FuGENE® 6 transfection reagent (Promega). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA was purified from minipreps with QIAprep spin Miniprep Kit (Promega), and constructs were verified by sequencing (Microsynth).
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bioRxiv - Biochemistry 2023Quote: ... they were transfected with indicated plasmids using FuGENE 6 transfection reagent (Promega). After another 24h ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was isolated using a PureYield Plasmid Midi Preparation Kit (Promega, USA) and clones verified via sequencing ...
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bioRxiv - Developmental Biology 2023Quote: ... the Cre plasmid was transfected using Fugene HD transfection reagent (Promega, E2311), then sorted out GFP+tdTomato− live cells by FACS (SONYMA900) ...
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bioRxiv - Cancer Biology 2023Quote: pRL-TK-renilla and pGL3 plasmids were obtained from Promega (Charbonnieres, France). pEF-cmyc-RIP140 and pEGFP-RIP140 have been previously described (31) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Properly sized products were ligated to the pGEM T-Easy plasmid (Promega), transformed into chemically competent DH5-alpha E ...
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bioRxiv - Microbiology 2023Quote: ... or 5 µL qPCR plasmid pJEB029 (diluted serially in lambda DNA (Promega)) were added ...
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bioRxiv - Cell Biology 2023Quote: ... we mixed 2500 ng of plasmid DNA with FuGene transfection reagent (Promega) and transfected 1 million Sf9 cells seeded in a 6 well plate ...
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bioRxiv - Bioengineering 2023Quote: ... Wizard® SV 96 Plasmid DNA purification system (Promega, Madison, WI, USA) was used for plasmid isolation ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were transfected with the plasmid using FuGENE HD (Promega, E2311). The indicated experiments were carried out 12–18 h after transfection ...