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Citations for Promega :
401 - 450 of 4498 citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and digested 16 hours at 37°C with trypsin (Promega) and stopped by addition formic acid (FA ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested by Trypsin/Lys-C Mix (Promega). After the digestion ...
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bioRxiv - Molecular Biology 2022Quote: ... firstly with Endoproteinase Lys-C (ratio 1:33 enzyme:lysate) (Promega) for 1 hour at room temperature then with trypsin (ratio 1:33 enzyme:lysate ...
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bioRxiv - Neuroscience 2023Quote: ... In-gel digestion with Trypsin/Lys-C Mix solution (Promega) was performed as previously described (Gonzalez-Lozano & Koopmans ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of 0.1 µg/µL Trypsin/rLys-C (Promega) in 100 mM ammonium bicarbonate (pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing-grade porcine trypsin/Lys-C mix (Promega, Madison, WI) was added ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 25L°C with trypsin (Promega) with an enzyme/substrate ratio of 1:250 ...
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bioRxiv - Molecular Biology 2023Quote: ... and then sequencing-grade endopeptidase Trypsin/Lys-C Mix (Promega) was added at an enzyme–substrate ratio of 1:50 wt/wt ...
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bioRxiv - Physiology 2023Quote: ... and digested (0.4 ug of Lys-C/Trypsin mix, Promega). After 4 hours of digestion in the urea buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with trypsin (Promega) and Lys-C (Wako,125-05061 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were then incubated with Lys-C/trypsin mixture (Promega) (200 ng for pull-down samples and 1 ug for total lysate samples ...
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bioRxiv - Biochemistry 2024Quote: ... and Glu-C (V1651) were obtained from Promega (Finnboda, Sweden).
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bioRxiv - Microbiology 2020Quote: ... RNA was reverse transcribed to cDNA using the ImProm-II™ Reverse Transcription System (Promega) and the synthesized cDNA was used for real-time analysis using the SYBR Green PCR Master Mix kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... and a Modulus II Microplate Multimode reader (Turner BioSystem) or a Glowmax Navigator luminometer (Promega), as described previously(24) ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse-transcriptase reactions were performed with the ImProm-II Reverse Transcription System (Promega, Madison, USA) using 2 μg total RNA ...
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bioRxiv - Molecular Biology 2019Quote: ... The cDNA was reverse-transcribed using the ImProm-II™ Reverse Transcription System (A3800; Promega). The expression levels of the ANRIL ...
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bioRxiv - Neuroscience 2020Quote: Total mRNA was isolated from 25 to 35 Drosophila heads following the RNeasy mini kit extraction protocol from Qiagen and reverse transcribed with oligo(dT)15 and the ImProm-II Reverse Transcription System (Promega) according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... 1.0 ug of total RNA was reverse-transcribed with ImProm-II reverse-transcriptase (Promega, USA) and random hexamers (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... the RNA was reverse transcribed to cDNA using the ImProm-II reverse transcription system (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized from nuclear and cytoplasmic RNA using Improm II RT (Promega, Cat. #A3800), and cDNA levels were measured by qPCR using the Brilliant III Ultra-Fast qPCR Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... First-strand cDNA synthesis was carried out with the ImProm-II Reverse Transcription System (Promega). Quantitative real-time PCR analysis was performed in a QuantStudio 5 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Neuroscience 2019Quote: ... Reverse-transcriptase reactions were performed with the ImProm-II Reverse Transcription System (Promega, Madison, USA) using 2 μg total RNA ...
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bioRxiv - Biochemistry 2022Quote: ... and (ii) HALO tag was incubated with primary Anti-HaloTag® Monoclonal Antibody (Promega #G9211) diluted 1:1 000 with secondary antibody Anti-Mouse IgG (Fc specific ...
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bioRxiv - Developmental Biology 2022Quote: ... one microgram of RNA was reverse transcribed using the ImProm-II Reverse Transcription System (Promega). Quantitative RT-PCR was performed in triplicate with custom-designed oligos using the CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... one microgram of RNA was reverse transcribed using the ImProm-II Reverse Transcription System (Promega), thereafter qRT-PCR was performed in triplicate with custom designed oligos (Table S3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and reverse transcription was carried out using the Improm-II reverse transcriptase (Promega, Medison, USA) under the conditions described in the kit manual ...
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bioRxiv - Genetics 2019Quote: ... and digestion were performed by dithiothreitol (DTT, 10mM, 30min at room temperature), iodoacetamide (IAA, 10mM, dark for 30min at 37°C) and trypsin (MS Grade, Promega, 12.5ng/ul, 37°C overnight) sequentially before analyzing by Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 min at 60°C) an alkylation (IAA 0.5 M, 30 min RT) proteins were digested using trypsin (Gold, Promega, 1ug / sample, overnight at 37°C). For LC MSMS analysis ...
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bioRxiv - Genomics 2022Quote: ... The primers were commercially synthesized (IDT) and tested on human genomic DNA (Promega) to confirm generation of only one amplicon product at the expected size ...
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bioRxiv - Genetics 2020Quote: ... Deidentified healthy donor DNA was obtained from Promega (Human Genomic DNA: Female, G152A). DNA from ALS cases (ND11836 and ND13803 ...
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bioRxiv - Cancer Biology 2020Quote: 2000bp human lncRNA-TANAR(ENST00000425110.1) promoter was cloned into PGL3 basic vectors (Promega). By mutating the crucial site of AR binding site in the lncRNA-TANAR 5’ promoter to EcoRI cutting site (-GAATTC) ...
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bioRxiv - Immunology 2022Quote: Human LRBA was cloned in seven different fragments into pCIneo FLAG vectors (Promega). These plasmids were kindly provided by Dr ...
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bioRxiv - Genomics 2022Quote: ... diluted to 1% in purified human genomic DNA (Promega, female, catalog No. G1521). Briefly ...
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bioRxiv - Biochemistry 2023Quote: A whole-cell protein extract from human K562-cells (Promega, V6941, lot 444583) was dissolved in 50 mmol/L Tris and 6.5 mol/L urea ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... for 1h at 37°C in presence of random hexamers (Promega). qPCR primers (supplement Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μl of Trypsin/Lys-C mix (0.5μg/μl, Promega, V5073) was added to each sample and incubated for 3 hrs at RT in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin (Promega). Digested peptides were purified using C18 spin columns (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin (Promega) using S-Trap (Protifi ...
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bioRxiv - Plant Biology 2021Quote: ... 5 μg/mL Mass Spec Trypsin/Lys-C Mix (V5073, Promega) and 1 mM DTT was added to the samples ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated overnight at 37°C with sequencing grade trypsin (Promega) added at a 1:50 enzyme:substrate ratio (wt/wt) ...
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bioRxiv - Cell Biology 2021Quote: ... digested overnight with Trypsin/Lys-C (1:20 w/w, Promega), desalted using C18 Hypersep Spin Column (Thermo) ...
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bioRxiv - Cell Biology 2020Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin (Promega). Digested peptides were cleaned up using C18 spin columns (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested with Trypsin/ Lys-C (Promega, WI, USA). According to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 7 and digested with 500 ng recombinant Lys-C (Promega) at RT while shaking at 1,400 rpm ...
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bioRxiv - Immunology 2022Quote: ... dehydrated and proteolytically cleaved overnight at 37 °C with trypsin (Promega). The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore ...
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bioRxiv - Biophysics 2021Quote: ... The LgBiT fragment amplified from pBiT1.1-C [TK/LgBiT] Vector (Promega) using KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Cell Biology 2022Quote: ... 2 µg Lys-C/Trypsin mix protease (Promega, Mass spec grade) were added to each sample (10 mM EPPS pH 8.5 ...
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bioRxiv - Cell Biology 2022Quote: ... in-gel tryptic digestion (250 ng Trypsin-Lys-C mix Promega V5072 in 50 mM ammonium bicarbonate overnight at 37oC) ...
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bioRxiv - Molecular Biology 2019Quote: ... for 1 hour at 37 °C with RNasin Plus (N2611, Promega). The RNA was extracted with Acid Phenol:Chloroform:Isoamyl alcohol (125:24:1 ...