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Citations for Promega :
451 - 500 of 3827 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: Purified NanoLuc protein (Promega REF G9711) was diluted to 10 µM in 0.1 mg/ml BSA solution ...
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bioRxiv - Cancer Biology 2019Quote: ... mice received 150 mg/kg D-Luciferin (Beetle luciferin, E1605; Promega, Charbonnières, France) intraperitoneally ...
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bioRxiv - Microbiology 2021Quote: ... injection of 150 mg/kg D-luciferin (Beetle Luciferin Potassium Salt, Promega, USA). Images were analyzed using LivingImage v4.3.1 software (PerkinElmer ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were left 48 hours and d-luciferin (Promega #E1605; dissolved in PBS) was added to the DMEM media in a concentration (150 µg/mL) ...
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bioRxiv - Cell Biology 2024Quote: ... the cell population was perfused with 25 µM beetle D-luciferin (Promega, E1601). Within 2–3 min ...
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bioRxiv - Cancer Biology 2024Quote: ... following intraperitoneal injections of 150 mg/kg D-luciferin (Promega, Alexandria, NSW, Australia) and under anaesthesia with isoflurane ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were dissolved in 40 mM ammonium bicarbonate and digested by 1 ug sequence-grade modified trypsin (Promega, Madison, WI, USA) at 37 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Heterologous expression of proteins was achieved by transfecting cells with 1 □g of plasmid DNA/35 mm using FuGENE HD (Promega; E2311), as previously described (Webb et al. ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then alkylated using 15mM chloroacetamide at 37°C for 30min and further digested using sequencing-grade modified trypsin (1/25, w/w, trypsin/protein; Promega, USA) at 37°C overnight ...
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bioRxiv - Biochemistry 2022Quote: Peptide mass spectrometry was completed after reduction with 10 mM DTT and alkylation with 55 mM iodoacetamide and protein digestion overnight at 37 °C at a 1:50 ratio of trypsin (Promega, UK). Tryptic peptides were analysed by nano-scale capillary LC-MS/MS with an Ultimate U3000 HPLC (ThermoFisher Scientific Dionex ...
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bioRxiv - Cell Biology 2021Quote: ... and 40 μg of proteins/sample was loaded onto S-trap columns (Protifi) and digested overnight with 1 μg sequencing grade modified trypsin (Promega, V5111) at 37°C ...
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bioRxiv - Systems Biology 2022Quote: ... The urea concentration was then diluted to 1 M with 50 mM ABC and the proteins digested with trypsin (Promega, V511c) at 1:100 mass-ratio at 37 °C overnight.
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bioRxiv - Immunology 2022Quote: ... Samples were diluted two-fold with 100 mM of triethylammonium bicarbonate (TEAB) and proteins were digested during 3 hours with 1 µg of trypsin (Promega V5111) and 1 µg of LysC (Wako 129-02541 ...
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bioRxiv - Systems Biology 2019Quote: ... The samples were then diluted with 0.1 M NH4HCO3 to a final concentration of 1 M urea and the proteins were digested with sequencing-grade porcine trypsin (Promega, Switzerland) at a final enzyme:substrate ratio of 1:100 (w/w) ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30 min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37° C for 12 hours ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The urea concentration was reduced to <2M and the protein sample was digested overnight at 37°C with 1:100 Trypsin Gold (Promega V5280). The following day ...
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bioRxiv - Physiology 2022Quote: ... Protein concentration in the supernatant was measured using a NanoDrop and 1:200 (w/w) of trypsin (Promega, Mass Spectrometry grade) was added to 200 µg of sample ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37°C for 12h ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 6 M guanidine hydrochloride.250 µg of protein were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Plant Biology 2021Quote: ... The ANAC50 protein was purified with the HaloTag® Protein Purification System (Promega, Madison, Wisconsin, United States) using 20 μL Expression reaction for each EMSA reaction. ...
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bioRxiv - Cancer Biology 2021Quote: ... 1500 μg total protein lysate was 100ul slurry of pre-cleared with Protein G agarose beads (Promega), followed by overnight incubation with AXL antibody (2ug Ab/ 100 ul lysate) ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified with MagneGST™ Protein Purification System (www.promega.com/protocols/; Promega, Madison, WI, USA).
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bioRxiv - Plant Biology 2024Quote: ... The soluble GST and His fusion proteins were purified using the MagneGST Protein Purification System (Promega, USA) or the MagneHis Protein Purification System (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant GST-OsNAC5 fusion protein was subsequently purified with the MagneGST™ Protein Purification System (Promega), following the provided protocol.
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bioRxiv - Cell Biology 2019Quote: ... and 5 ng/mL recombinant human basic fibroblast growth factor (bFGF, Promega) then centrifuged ...
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bioRxiv - Cell Biology 2023Quote: ... human BLTP2/KIAA0100 ORF was amplified from pFN21A-Halo-KIAA0100 (FHC00016, Promega). An unstructured linker (sequence ...
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bioRxiv - Molecular Biology 2020Quote: ... The bacterial surface proteins with attached plasma proteins were released by limited proteolysis with 2 μG trypsin (Promega) /37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was then incubated at 37°C for overnight trypsin digestion (trypsin 1:50 protein w/w ratio; Promega, Madison, WI). The digestion reaction was quenched by adding formic acid (FA ...
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bioRxiv - Biochemistry 2020Quote: ... Trypsinisation was carried out by incubating the gel pieces overnight at 37 °C in 50 µl of trypsin (1: 25:: trypsin: protein, 20 µg/ml, sequencing grade trypsin V5111, Promega, Madison, WI) prepared in ammonium bicarbonate (50 mM) ...
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bioRxiv - Cell Biology 2020Quote: ... The medium was then replaced with fresh medium in the incubator and protein release was induced by biotin addition (100 µM final) and following Furmazin (Promega, 1/500) addition ...
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bioRxiv - Developmental Biology 2019Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the medium into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated overnight at 25 °C or endoproteinase GluC in a GluC/protein ratio of 1/20 (w/w) (Promega Cat. No.V1651) and incubated overnight at 37 °C ...
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bioRxiv - Physiology 2022Quote: ... protein samples were digested by incubated overnight at 37°C with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin). Samples were acidified using 5% TFA and peptides cleaned up using the Phoenix 96× kit (PreOmics ...
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bioRxiv - Neuroscience 2022Quote: ... Protein digestion was processed for overnight by adding LysC and trypsin in a 1:50 ratio (µg of the enzyme to µg of protein; Promega; Cat#: V5071)) at 37° C and 1400 rpm ...
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bioRxiv - Developmental Biology 2022Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the media into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... after which growth media was removed and 20 µl of 4% D-luciferin (Promega) in CO2 independent medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: iPSCs were exposed to 0.3 mg/l D-luciferin (Promega, Benelux, Leiden, The Nederlands). Images were obtained immediately after D-luciferin application with the IVIS® Spectrum (Caliper Life Science ...
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bioRxiv - Cancer Biology 2022Quote: ... mice were injected subcutaneously with 75 mg/kg D-luciferin potassium salt (Promega E1605) in sterile PBS and anesthetized with 2% isoflurane in medical air ...
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bioRxiv - Cancer Biology 2020Quote: ... mice were injected intraperitoneally with 150 mg/kg body weight of d-luciferin (Promega) and 10 minutes later the mice were imaged using a Xenogen IVIS Lumina system with Living Image version 3.0 software (Caliper Life Sciences) ...
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bioRxiv - Microbiology 2023Quote: ... Mice were intraperitoneally injected with 200 μl of 40 mg/ml D-luciferin (Promega) in PBS and anesthetized with isoflurane ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were digested with trypsin (Promega Corporation) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with 1.5μg Trypsin (Promega) at 37°C for 12 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested overnight with trypsin (Promega) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were first digested with chymotrypsin (Promega) (12.5 ng/µl ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were digested overnight with trypsin (Promega). The resulting peptides were labelled with the TMT sixplex isobaric mass tag ...
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bioRxiv - Systems Biology 2020Quote: ... Proteins were then digested with trypsin (Promega) on-beads as previously detailed (66) ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were digested overnight using trypsin (Promega, Sequencing Grade Modified Trypsin ...
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bioRxiv - Systems Biology 2022Quote: ... Proteins were then digested with trypsin (Promega) overnight at 37°C with an enzyme trypsin ratio of 1:50 ...
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bioRxiv - Plant Biology 2022Quote: ... Protein digestion was done with trypsin (Promega) at a 1:50 ratio and incubated for 4 h ...