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Citations for Promega :
1 - 50 of 247 citations for Human IL 17 Receptor since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Lumit Human IL-1β Immunoassay (Promega) was performed according to manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were co-transfected with pcDNA5/FRT construct encoding haemagglutinin (HA)-tagged human CB1 receptor cDNA and pOG44 (Flp recombinase plasmid) using transfection reagent Fugene HD (Promega) as previously described for AtT-20 pituitary tumour cells [26] ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
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bioRxiv - Biochemistry 2022Quote: ... and M75F receptor plasmids using FuGene HD (Promega). Cells were treated with DMSO or varying drug concentrations 24 hours after transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... 17 Synergy assessments were performed using CellTiter-Glo (Promega) as previously described26 with the following modifications ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and thereafter digested with 17 ng/μl sequencing-grade trypsin (Promega) in 25 mM NH4HCO3 using a slightly modified in-gel digestion protocol (Shevchenko et al ...
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bioRxiv - Molecular Biology 2019Quote: ... and finally digested with 17 ng/μl sequencing-grade trypsin (Promega) in 25 mM NH4HCO3 using a slightly modified in-gel digestion protocol (Shevchenko et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... and finally digested with 17 ng/μl sequencing-grade trypsin (Promega) in 25 mM ABC ...
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bioRxiv - Cell Biology 2020Quote: ... 293T/17 and HeLa cells were transfected with plasmids using FugeneHD (Promega) or Lipofectamine3000 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1.5 μg receptor and 1 μg GloSensor-22F (Promega). After 24 hours ...
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bioRxiv - Immunology 2021Quote: ... After 17-20 h 50 μL/well of One-Glo-EX substrate (Promega) was added to the cells and incubated in the dark for 5-10 min prior reading on a Varioskan LUX plate reader (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... After 17-20 hours VSV 40 μL/well of One-Glo-EX substrate (Promega) was added to the cells and incubated in the dark for 5-10 min prior reading on a BioTek plate reader ...
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bioRxiv - Biochemistry 2022Quote: HEK293 T cells were co-transfected with the target receptor construct and GloSensor cAMP reporter (Promega) in DMEM supplemented with 10% FBS for overnight incubation ...
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bioRxiv - Biochemistry 2024Quote: lrl 1μg of N-terminally FLAG-tagged receptor and 1μg of F22 (Promega, Cat. no: E2301) (for GloSensor assay)
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bioRxiv - Cancer Biology 2022Quote: ... luciferase activity was measured as previously described (17) using the Dual-Glo Luciferase assay system (Promega) using a Promega GloMax Explorer multimode plate reader.
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bioRxiv - Molecular Biology 2021Quote: ... was digested with the same enzymes and ligated with the amplified receptors using T4 DNA ligase (Promega).
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bioRxiv - Zoology 2020Quote: ... rehydrated with 17 μl 25 mM ammonium hydrogen carbonate containing 4.5 ng/μl sequencing grade trypsin (Promega) and 0.025% Proteasemax (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293 cells were transfected with FLAG-tagged receptor (3.5 μg) and luciferase-based 22F cAMP biosensor construct (3.5 μg) (Promega). 14–16 h post transfection ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTag-receptor expressing mESCs were fluorescently labeled with either 1 µM of HaloTag AlexaFluor 488 ligand (Promega, G1001) or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: To measure ATP bound to detergent purified P2X1 receptor a Kinase-Glo® Luminescent Kinase Assay (ProMega, USA) was used ...
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bioRxiv - Bioengineering 2020Quote: ... Unstimulated media (+CTRL) contained complete α-MEM medium only and stimulated media (+IL-1β) contained 20 ng/mL IL-1β (FHC05510, Promega, Madison, WI, USA) in complete α-MEM medium ...
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bioRxiv - Biochemistry 2020Quote: Human ClCN1 cDNA was expressed from pCDNA 3.1(+) expression vector in HEK293T/17 cells (ATCC) by transient transfection using Fugene 6.0 reagent (Promega) according to the manufacturer’s specifications ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (1.764 µg) were also transfected into HEK293T cells cultured on acid-washed coverslips using FuGENE HD (Promega) in Optimem (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 2 μg of FLAG-tagged β2 or β1 adrenergic receptor DNA using Fugene 6 (Promega) and seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips 4-8 hours after transfection.
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bioRxiv - Biophysics 2023Quote: ... HEK293T cells were co-transfected with FLAG-tagged receptor (3.5μg) and luciferase-based 22F cAMP biosensor construct (3.5μg) (Promega, Cat. no: E2301) using polyethyleneimine (PEI ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...
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bioRxiv - Genetics 2020Quote: ... HEK293T/17 cells were transfected with firefly luciferase reporter constructs and a Renilla luciferase (Rluc) normalization control (pGL4.74; Promega) in a ratio of 50:1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were seeded in a 10cm cell culture dish and were transiently transfected with 17 μg of plasmid cDNA (pcDNA 3.4 TOPO) and 53 μl Fugene HD (Promega) in 10 ml of HEK293 media ...
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bioRxiv - Molecular Biology 2023Quote: ... S0ng of parental plasmid was amplified with complementary 35nt-long forward and reverse primer pairs (0.25uM each) containing the desired nucleotide substitution (at nt position 17) in the presence of 1.25U Pfu DNA polymerase (Promega) and 200uM dNTPs (each ...
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bioRxiv - Molecular Biology 2022Quote: ... Human Genomic DNA (G1521, Promega) was used as positive control and synthetic DNA was used to validate the LAMP assay performance ...
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bioRxiv - Biophysics 2021Quote: ... sapiens TACAN-eGFP or the M2 muscarinic receptor (Halo-M2R-eGFP) plasmid (previously established in this lab) was transfected into the cells using FugeneHD (Promega) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were co-transfected with FLAG-tagged receptor constructs and luciferase-based 22F cAMP biosensor (Promega, Cat. no. E2301) using polyethylenimine (PEI ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was used to amplify the coding regions of the steroid receptor genes and to linearize the expression plasmids pHTN HaloTag CMV Neo or pFLN-1 NanoLuc (Promega #N1811 ...
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bioRxiv - Neuroscience 2023Quote: Surface expression was measured using a HiBit-tagged 5-HT2A receptor and the Nano-Glo HiBit Extracellular Detection System (Promega). N-terminal HiBit-tagged human 5-HT2A receptor was cloned into pcDNA3.1 using Gibson Assembly ...
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bioRxiv - Cell Biology 2023Quote: Protein-protein interactions between Mkl1/2 and Foxo1/3/4 or glucocorticoid receptors were also measured using NanoBiT PPI Starter Systems (Promega). 293FT cells were seeded in 96-well white wall microplates and co-transfected with the LgBiT and SmBiT plasmids ...
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bioRxiv - Biochemistry 2024Quote: ... GRK371 and β-arrestin272 recruitment to chemokine receptors (CXCR1, CXCR2, CXCR3-A, CXCR4, CXCR5, CXCR6 and ACKR3) was monitored using a nanoluciferase complementation-based assay (NanoBiT, Promega)73,74 ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged receptor and 3.5μg of an SRE-based luciferase reporter plasmid pGL4.33 (Promega, Cat. no: E1340). 14-16h after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-NPHP2 and GFP-NPHP5 expression vectors were transiently transfected to 293T/17 cells together with pCS2FLAG empty vectors (as a control) or pCS2FLAG-NPHP1 variants using FuGENE HD (Promega), following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... and then a final solution comprised of 17% formamide + 5% Triethalomine + 70% glycerol + 3% Tris (w/v, Tris, Promega, H5135) made in basic DDH20 ...
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bioRxiv - Microbiology 2020Quote: ... 500 ng human genomic DNA (Promega) or 5 μl of untreated or RNase-treated nasal swab nucleic acid isolates ...
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bioRxiv - Cancer Biology 2020Quote: ... Pooled human male genomic DNA (Promega) was randomly sheared (500-800bp ...
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bioRxiv - Systems Biology 2021Quote: ... a human cDNA pool (Promega Corporation), or obtained as synthesized double-stranded DNA fragments (gBlocks ...
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bioRxiv - Genomics 2021Quote: ... 50ng human genomic DNA (Promega, G304A), 2µM assembled T7-Tn5 transposase or Tn5 transposase was incubated at 55 °C for 7minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... human Female genomic DNA (Promega #G1521), as a reference ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 8×106 HEK293T/17 cells were transfected with a proviral backbone plasmid and an envelope expression vector by using FuGENE HD (Promega, Madison, WI) and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... L981F. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega, Catalog #E2692). Pseudovirions for 293T/ACE2 infection were produced by co-transfection with a lentiviral backbone (pCMV ΔR8.2 ...
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bioRxiv - Biochemistry 2020Quote: ... The mutation was confirmed by full-length Spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega Cat#E2692) and a combination of Spike plasmid ...