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Citations for Promega :
151 - 200 of 417 citations for Human CLEC4M L SIGN His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and human Renilla luciferase (pGL4.74[hRluc/TK], Promega, E6921) were used in the study ...
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bioRxiv - Microbiology 2020Quote: ... insert (NRP1, native human ORF in pF1K, FXC23812, Promega): 5’-atagggcgaattcggtagtaaggagcgatcgc-3’ (fwd ...
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bioRxiv - Cancer Biology 2022Quote: ... were amplified by PCR from human genomic DNA (Promega), using the primers listed in Table S1 and then inserted into the psiCHECK-2 vector ...
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bioRxiv - Biochemistry 2022Quote: ... human GR and MR were cloned into pACT (Promega) as N-terminal VP16 fusions using HiFi assembly (NEB) ...
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bioRxiv - Cell Biology 2019Quote: ... A cDNA encoding human KIF1A was purchased from Promega Japan (Promega Japan ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Human PAR2 with N-terminal nano-luciferase (nLuc, Promega) and C-terminal enhanced Yellow Fluorescent Protein (eYFP ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant His-MBP-AlpA and His-MBP at a concentration of 10 μM was incubated with 100 μl MagneHis™ Ni Particles (Promega) equilibrated with pulldown-buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... MSCV-IRES-eGFP and MSCV-IRES-tagBFP vectors respectively in frame with 3xHA tag using EcoRI/BamHI digestion (Promega, R6011/Promega, R6021), gel purification ...
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bioRxiv - Biophysics 2019Quote: ... Amino-PEG-QDs (Thermo, Q21521MP) were labelled with HaloTag ligand by reaction with N-hydroxysulfosuccinimide reactive Halo-Tag ligand (Promega, P6751) or Snap-Tag ligand for 30 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: mRNAs of C9orf72 intron1-(G4C2)80 with a Myc tag in the GA frame were in vitro translated with Flexi® Rabbit Reticulocyte Lysate System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: The coding sequence or DNA-binding domain of the gene of interest was cloned with Gibson assembly in frame with the Halo-tag into the vector pFN19A (Promega, discontinued), which contains a SP6/T7 promotor ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-rabbit IgG (H+L) HRP conjugate (Promega #W4011, 1:10000), mouse anti-Alix (Abcam #ab117600 ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-mouse IgG (H+L) HRP conjugate (Promega #W4021, 1:10000), anti-rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2020Quote: ... CaCl2·2H20 (0.99 g/L)) supplemented with 0.05% Tween 20 (Promega), 2.5 mM DTT ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-mouse IgG (H+L) HRP conjugate (Promega #W4021, 1:10000), anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-rabbit IgG (H+L) HRP conjugate (Promega #W4011, 1:10000), anti-goat IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... anti-mouse IgG (H+L) HRP conjugate (Promega #W4021, 1:10000), anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... anti-rabbit IgG (H+L) HRP conjugate (Promega #W4011, 1:10000).
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bioRxiv - Molecular Biology 2021Quote: The CD19 minigene was amplified from human genomic DNA (Promega) with the primers 5’-catAAGCTTgaccaccgccttcctctctg-3’ and 5’-catGAATTCNNNNNNNNNNNNNNNGGATCCttcccggcatctccccagtc-3’ ...
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bioRxiv - Neuroscience 2020Quote: ... the human CB1R was cloned into the pCI vector (Promega), and eCB2.0 and eCBmut were cloned into the peGFP-C1 vector (Takara) ...
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bioRxiv - Immunology 2022Quote: ... and a β-globin calibration curve (human genomic DNA [Promega]) was concurrently evaluated on each plate ...
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bioRxiv - Microbiology 2020Quote: ... The human codon-optimized NanoLuc Luciferase reporter gene (Nluc, Promega) was inserted in place of nucleotides 1-100 of the nef-gene ...
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bioRxiv - Genomics 2022Quote: ... 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 μM dexamethasone (Sigma-Aldrich) ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... donovani BPK282/0 cl4 promastigote DNA with human DNA (Promega) from 1/15 to 1/150000 (Leishmania:human ...
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bioRxiv - Immunology 2020Quote: ... The human codon-optimized nanoluc Luciferase reporter gene (Nluc, Promega) was inserted in place of nucleotides 1-100 of the nef-gene ...
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bioRxiv - Molecular Biology 2022Quote: ... Serial dilutions of genomic DNA from human placenta (G1471, Promega) were used as a standard for quantification and their concentration.
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bioRxiv - Microbiology 2022Quote: ... falciparum 3D7 DNA with commercially available human DNA (Promega, G304A) to a total amount of 400ng DNA ...
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bioRxiv - Neuroscience 2024Quote: Human male genomic DNA obtained from Promega (Ref:G1471; Madison, WI) was used to generate the input library ...
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bioRxiv - Microbiology 2021Quote: ... The CaptureSelect Biotin anti-C-tag Conjugate (LifeTechnologies #7103252100; 1/4000) was mixed with Streptavidin Alkaline Phosphatase (Promega #V5591; 1/1000 dilution) for 10 minutes before use ...
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bioRxiv - Molecular Biology 2022Quote: ... MSCV-IRES-eGFP and MSCV-IRES-tagBFP vectors respectively in frame with 3xHA tag using EcoRI/BamHI digestion (Promega, R6011/Promega, R6021), gel purification ...
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bioRxiv - Microbiology 2019Quote: ... we replaced the C-terminal V5 tag with either HaloTag or NanoLuc (NLuc) from the NanoBRET Nano-Glo Detection System (Promega, Madison, WI). As a negative control ...
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bioRxiv - Biophysics 2023Quote: HeLa cells expressing each Halo-tagged chromatin remodeler were labeled with 5 nM Janelia Fluor® 549 (JF549) Halo-tag® ligand (GA1110, Promega) for 15 min at 37 °C ...
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bioRxiv - Biophysics 2023Quote: ... Halo-EGFR constructs used for FRET-FLIM experiments were created using the HA-EGFR plasmid and the Gibson assembly approach to replace the N-terminal HA tag with the HaloTag® plasmid (Promega, #G7711). The final resulting construct contained the HaloTag and a GSGS linker region after the EGFR signal sequence ...
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bioRxiv - Plant Biology 2023Quote: The protoplast samples with the HiBiT tag were analyzed using a Nano-Glo® HiBiT Lytic Detection System (Promega Corp., Madison, WI). As per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 1 mM L-Glutamin and 250 μM Luziferin D (Promega, Madison, USA). Cells were incubated 12-16 hours in a humidified incubator at 37°C and 5% CO2 to adhere to the bottom of the plate ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-oligo d(T) (Promega, #Z5261, 0.2 μmol/L for mRNA FISH); Diethyl Pyrocarbonate (DEPC)-treated water (Invitrogen™ ...
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bioRxiv - Cancer Biology 2020Quote: ... pT7-IRES His-T7-HCF-1VIC was in vitro transcribed/translated using the TnT Quick Coupled Transcription/Translation System (Promega, Madison, Wisconsin, L1171). Two milligrams recombinant MYC and 12 μl T7-HCF-1VIC were rotated overnight at 4°C in Kischkel buffer + PIC (Roche 05056489001) ...
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bioRxiv - Microbiology 2021Quote: ... The linearized vector and the slpMh-6x His fragment were gel-purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany) and assembled via isothermal in vitro ligation according to Gibson et al ...
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bioRxiv - Molecular Biology 2020Quote: ... and pooled human male DNA (G3041, Promega and 4312660, Life Technologies) were used to generate calibration curves and serve as reaction controls ...
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bioRxiv - Biochemistry 2020Quote: SETD2-HaloTag® human ORF in pFN21A was procured from Promega. Deletion mutants of SETD2 were constructed by PCR (Phusion polymerase ...
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bioRxiv - Biochemistry 2020Quote: SETD2-HaloTag® human ORF in pFN21A was procured from Promega. Deletion mutants of SETD2 were constructed by PCR (Phusion polymerase ...
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bioRxiv - Cancer Biology 2022Quote: ... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
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bioRxiv - Cell Biology 2021Quote: ... Human normal genomic DNA was obtained from Promega (Madison, WI, USA) and the values are averages of three replicates ...
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bioRxiv - Biophysics 2021Quote: ... and 5 ng/ml recombinant human fibroblast growth factor (G5071, Promega).
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bioRxiv - Cancer Biology 2020Quote: ... in a background of 130ng of normal human genomic DNA (Promega) was used as a positive control ...
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bioRxiv - Cell Biology 2019Quote: ... and human samples were performed using GoTaq green master mix (Promega) and the following amplification conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... 830bp or 800bp human STIM1 promoter into pGL3 basic vector (Promega) between XhoI and HindIII ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... Proteins were expressed from the pcDNA™3.1/His construct by the TNT® T7 Quick Coupled Transcription/Translation kit (Catalog #L1170, Promega Co., Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... Eight-repeats of protein L were inserted into a modified pFN18a vector (Promega), which introduces a HaloTag at the N-terminus and a SpyTag at the C-terminus ...
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bioRxiv - Cell Biology 2021Quote: ... and then anti-mouse IgG (H&L) alkaline phosphatase-conjugated secondary antibodies (Promega) were incubated with the blots at a dilution of 1:5,000 for 1 h at room temperature (RT) ...