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Citations for Promega :
601 - 650 of 1446 citations for Herpes Simplex Virus 2 Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... We performed targeted PCR by adding 1 μL of cell lysate (1:5 dilution) to a 25-μL PCR reaction containing GoTaq Hot Start Master Mix Green (Promega) and 0.5 μL of the primers (10 µM ...
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bioRxiv - Biochemistry 2022Quote: ... Luciferase activity was determined 18-24 hr post-transfection from cell lysate using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturers protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... the luminescence of 50 µl of cell lysate was measured using the Nano-Glo Luciferase Assay kit (Promega, Madison, WI) as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of the lysate were transferred into two Microtiter plates (one for Renilla and the second for Bright Glo (Promega)) ...
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bioRxiv - Cell Biology 2023Quote: ATP was measured in MPs or cell lysates with the luciferase/luciferin method (Enliten rluciferase/luciferin cat. # FF2021, Promega, Italy) in a Firezyme luminometer (Biomedica Diagnostics Inc ...
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bioRxiv - Microbiology 2023Quote: ... and whole cell lysates prepared and assayed for luciferase activity using the Dual Luciferase Assay System as per manufacturer’s directions (Promega, USA). The luminescence signal was detected using the luminometer (Promega) ...
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bioRxiv - Biochemistry 2023Quote: In vitro translation of DHX15-G4-FL and DHX15-G4-Del-FL transcripts in the presence of F1 or BRACO19 was carried out in a cell-free translation system of rabbit reticulocyte lysates (Promega) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... was removed by evaporation using speed-vac and the residue was used to produce L-[2,3,4,5-3H]-proline labeled procollagen chains and the collagen-like proteins in the TnT® Coupled Reticulocyte Lysate Systems (Promega) using either SP6 or T7 promoters ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of the lysates were used for the quantification of Firefly and Renilla Luciferase activity using a Luminometer (GloMax, Promega).
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of the lysates were used for the quantification of Firefly and Renilla Luciferase activity using a Luminometer (GloMax, Promega). The ratio between Renilla and Firefly is calculated for each experimental condition.
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bioRxiv - Immunology 2023Quote: ... Protein expression in luciferase-based assays was verified by analyzing the corresponding lysates stored in 1x passive lysis buffer (Promega) by Tricine-SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were harvested and the activities of firefly and Renilla luciferase were measured in the same cell lysate using the Dual-Luciferase Reporter Assay System (Promega) and GloMax Luminometer (Promega) ...
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bioRxiv - Genetics 2023Quote: ... The HALO-NAC61 and GST-HALO (used as negative control) fusion proteins were translated in vitro using the TNTR SP6 coupled reticulocyte lysate system (Promega). Two replicates were used for the TF and input libraries ...
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bioRxiv - Developmental Biology 2023Quote: ... Oct4 was transcribed and translated in vitro in the presence of 35S-methionine (TNT Coupled Reticulocyte Lysate System, Promega, USA) according to the manufactureŕs protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and luciferase activities of cell lysate were recorded by using a dual-luciferase reporter assay system (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... The final 50 µL translation reaction included the 5 µL RNA mixture described above and components from the Rabbit Reticulocyte Lysate system (Promega): 35 µL of rabbit reticulocyte lysate ...
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bioRxiv - Neuroscience 2023Quote: ... 1µl lysate was diluted with 39 µl PBS before being mixed with 40 µl of Dual-Glo FLuc substrate (Promega). After 10 min incubation ...
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bioRxiv - Genetics 2023Quote: ... Luciferase activity in cell lysates was assayed 48 hours post-transfection using the Dual Luciferase Reporter Assay kit (Promega E1910), and the Fluoroskan Ascent FL microplate reader (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... LDH activity in the supernatants and the cell lysates was then assessed using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega) by following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μL of the cell lysate was transferred into a white 96-well plate and 30 μL of firefly luciferase substrate (Promega) was added ...
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bioRxiv - Cancer Biology 2023Quote: Caspase3/7 activity was quantified in cell lysates using the Kit Caspase-Glo® 3/7 Assay Systems (G8091, Promega).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Wells containing single cell colonies were duplicated into 12-well plates and screened for HiBit expression by mixing crude lysates with purified LgBit protein (Promega) and measuring luminescence in the presence of 5 μM furimazine ...
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bioRxiv - Microbiology 2024Quote: In vitro transcription and translation reactions using rabbit reticulocyte lysates as previously described (29) were performed by TNT Quick Coupled Transcription/Translation System (Promega) in the presence of [35S] Met (Perkin-Elmer) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were freeze-thawed and 25 μL of lysate from each well was added to 25 μL of firefly luciferase reagent (Bright-Glo, Promega), and 10 μL of each lysate was added to 50 μL of 2 μg/mL Renilla luciferase substrate (NanoLight Technology) ...
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bioRxiv - Molecular Biology 2024Quote: ... at 48-72 hrs post-transfection and lysates were assayed for luciferase activity using the dual-luciferase reporter assay system (Promega) and a luminometer ...
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bioRxiv - Cancer Biology 2023Quote: ... Alkylated proteins were then cleaved using a two-step digestion: first with Endoproteinase Lys-C (ratio 1:33 enzyme: lysate, Promega) for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme ...
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bioRxiv - Cell Biology 2024Quote: ... were cloned into the pSP64 in vitro translation vector and translated with a 35S radiolabelled methionine label using the TNT coupled reticulocyte lysate system (Promega) following manufacturers guidelines ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 µl of random primers (Promega) were added after which the mixture was incubated at 65 °C for 5 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2 μg of Trypsin (Promega, V5111) was added to each sample and they were incubated shaking at 37°C overnight (o/n) ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.25 µl of 2% digitonin (Promega) and 0.5 µl of 10% Tween-20 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 U GoTaq Flexi Polymerase (Promega) and 0.22 µg TaqStart Antibody (Clontech) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μL Superasin RNase inhibitor (Promega), and 8 μL PEG8000 (supplied with ligase) ...
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bioRxiv - Molecular Biology 2020Quote: The commercial psiCHECK™-2 (Promega) vector was modified by deleting the Rluc poly(A ...
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bioRxiv - Biochemistry 2021Quote: ... 2 U μL-1 RNasin (Promega), 1 mM ATP/GTP/CTP ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 U/μl RNaseIn Plus (Promega) and 10 U/μl SuperScript IV Reverse Transcriptase enzyme while heated ...
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bioRxiv - Neuroscience 2022Quote: ... The psiCHECK-2 vector (Promega, C8021) was used to build the dual luciferase reporters with Kcnj10 UTRs ...
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bioRxiv - Systems Biology 2022Quote: ... 2 µl trypsin (Promega Trypsin Gold) was added pre-diluted in ultra-pure water to 2 ng/µl and incubated overnight at 37°C in the thermal cycler ...
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bioRxiv - Cell Biology 2023Quote: ... The psiCHECK-2 vector (Promega, C8021) and the psiCHECK-RL-Ifnb1 3’ UTR vector were described before (Zhang et al. ...
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bioRxiv - Cell Biology 2020Quote: ... The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... as well as Tagged-BAF15 were synthesized using the TNT coupled reticulocyte lysate system according to the manual (Promega, L5020, USA). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: hNAA40 and dTIS mutagenized hNAA40 pOTB7 constructs were used as templates for in vitro coupled transcription/translation in a rabbit reticulocyte lysate system according to the manufacturer’s instructions (Promega, Madison, WI) to generate [35S] methionine labeled translation products ...
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bioRxiv - Molecular Biology 2021Quote: ... In vitro translation reactions were carried out with 5 ul of either supplemented rabbit reticulocyte lysate or oocyte extract in total volume of 20 µl containing 10 µM amino acid mix (Promega, L4461), 0.5 units SUPERase•In ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μL of cell lysate was distributed to mix with 25 μL of Nano-Glo Luciferase Assay System (Promega, Cat.# N1120), and another 25 μL of cell lysate was mixed with 25 μL of ONE-Glo Luciferase Assay System (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
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bioRxiv - Systems Biology 2019Quote: ... lysates were diluted in 700µl of TBS and incubated at 4°C with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with the same volume of Nano-Glo® Luciferase reagent (Promega). For the A-MLV titers ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... transduction efficiency was quantified by measuring the activity of firefly luciferase in cell lysates using a luciferase assay system (Promega, USA) and SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices ...
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bioRxiv - Cell Biology 2021Quote: In vitro translated (IVT) proteins were generated in rabbit reticulocyte lysate using the SP6 TNT Quick-Coupled Transcription/Translation kit (Promega, L2080) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Transduction efficiency was assessed by quantifying luciferase activity in cell lysates using a commercial kit (Luciferase Assay System, Promega, Cat. #E1500) and a plate-reading luminometer (SpectraMax i3x ...
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bioRxiv - Neuroscience 2021Quote: ... For HiBiT detection, 25µL of HiBiT lytic reagent (1:25 substrate, 1:50 LgBiT) was added to the lysate (Promega, cat# N3050). For FFluc detection ...