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Citations for Promega :
1 - 50 of 923 citations for Glycine N Fmoc 2 13C 99%;15N 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected in 96-well plates with 98 ng rTetR plasmid and 2 ng pRL Renilla Luciferase control reporter plasmid (Promega) to control for transfection efficiency ...
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bioRxiv - Microbiology 2023Quote: ... for 2 hours at 72 hours and lysed at 98 hours using 25 μl Dual-Glo® Luciferase Assay System (Promega). Luminescence was measured ...
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bioRxiv - Genomics 2024Quote: ... and quenched with 0.125 M Glycine (Promega) for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... β-arrestin 2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin 2; Promega, plasmid no. CS1603B118) was chosen as it has previously been used successfully with other class B GPCRs ...
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bioRxiv - Immunology 2022Quote: ... and digested using filter-aided sample preparation 98 with sequencing grade modified porcine trypsin (Promega). Tryptic peptides were separated by reverse-phase XSelect CSH C18 2.5 µm resin (Waters ...
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bioRxiv - Biochemistry 2019Quote: ... N-glycans were released from the membrane-bound protein using 2 U PNGase F (Promega, WI, USA) with overnight incubation at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega custom assay services (plasmid CS1603B118) ...
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bioRxiv - Cell Biology 2021Quote: HEK293 T-REx SNAPf-GLP-1R-SmBiT cells were seeded in 12-well plates and co-transfected with 0.1 μg β-arrestin-2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin-2; Promega, plasmid no. CS1603B118) and either 0.1 μg mini-Gs-Venus or mVenus supplemented with 0.8 μg pcDNA 3.1.
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bioRxiv - Biochemistry 2020Quote: N-glycans were released from nMPO using Elizabethkingia miricola peptide-N-glycosidase F (Promega) (Jensen et al. ...
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were enzymatic released using 3.5 U Flavobacterium meningosepticum N-glycosidase F (Promega) in 20µl water/well ...
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bioRxiv - Microbiology 2020Quote: ... In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously26 ...
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bioRxiv - Immunology 2022Quote: ... In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega (plasmid no. CS1603B118). Construction of the GLP-1R-SmBiT plasmid was described previously (55 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plasmid for β-arrestin-2 fused at the N-terminus to LgBiT was obtained from Promega (plasmid no. CS1603B118); this configuration was used due to previous success with another class B GPCR (Shintani et al. ...
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bioRxiv - Microbiology 2022Quote: In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously [25] ...
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bioRxiv - Microbiology 2022Quote: In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously20 ...
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bioRxiv - Molecular Biology 2022Quote: ... pNLF1-N [CMV Hygro] (Promega), and pNLF1-C [CMV Hygro] (Promega ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted individually from whole bees (test; n=300, control; n=165) by Promega Wizard® purification.
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bioRxiv - Microbiology 2020Quote: ... venezuelae macrocolonies that were grown for 30 h at 30°C on MYM agar were pooled and resuspended in 200 µl ice-cold stop solution (5% phenol (pH 4.3) in 98% ethanol) and RNA was isolated using the SV Total RNA Isolation Kit (Promega). After elution ...
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Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... An N-terminal HALO tag (Promega) for fluorescent labelling was fused to the coding sequence through a GGSGGSLQ linker ...
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bioRxiv - Neuroscience 2023Quote: ... and pBiT2.1-N [TK SmBiT] (Promega) vectors ...
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bioRxiv - Biophysics 2022Quote: pHalo-PPARγ2 expresses human PPARγ isoform 2 fused to HaloTag in the N-terminus under a CMVd1 promoter (Promega ORF clone #FHC08305). PPARγ2 mutants were generated by nucleotide substitution using the QuikChange II XL Site Directed Mutagenesis Kit (Stratagene ...
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bioRxiv - Cancer Biology 2022Quote: ... and pNLF1-N-CMV-Hygro (Promega, N1351) were obtained from Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing grade trypsin (Promega, P/N V511A) was used for the generation of tryptic peptides.
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bioRxiv - Cancer Biology 2022Quote: ... The N-glycans were then released using 10 U recombinant Elizabethkingia miricola N-glycosidase F (Promega, V4831, 10 U/μL) for 16 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: The N protein of SARS-CoV-2 B.1 D614G variant (NYI67-20 strain) was cloned into Nano-Luc vector pNLF1-N [CMV/Hygro] (N terminus) (Cat. N1351, Promega) as a fusion protein ...
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bioRxiv - Genomics 2020Quote: ... and unmethylated lambda DNA (Promega, Cat. N: D1521) was added to the samples (∼ 0.01%) ...
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bioRxiv - Microbiology 2022Quote: ... coli JM109 (Competent Cells, Promega, cat. n° L2005) and recombinant clones were checked by sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... N-terminal small bit (SmBiT) tagged construct (Promega), with the native HSV-TK promoter swapped out for a CMV promoter ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 5 min quenching with 100 mM glycine and permeabilization with 0.5% Triton X-100 (Promega, Madison, WI) in 1 × PBS for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... Trypsin Gold was obtained from Promega (Cat. N° V5280) (Madison ...
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bioRxiv - Cell Biology 2020Quote: ... followed by 20 μl Stop n Glo substrate (Promega) diluted 1:1 with filtered dH2O ...
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bioRxiv - Biochemistry 2020Quote: ... Asp-N (V1621) and Chymotrypsin (V1091) from Promega (USA) and Lys-C (125-05061 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Human PAR2 with N-terminal nano-luciferase (nLuc, Promega) and C-terminal enhanced Yellow Fluorescent Protein (eYFP ...
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bioRxiv - Cell Biology 2019Quote: ... and into N’3xGFP and N’Halo (Promega, Madison, WI) pCS2+ with BspE1 and Xho1 ...
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bioRxiv - Genomics 2020Quote: ... 0.1% RNAsin plus RNase inhibitor (Promega, Cat. N: N261A) in PBS (wash buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... and Asp-N were purchased from Promega (Madison, WI), and a-lytic protease was obtained from New England BioLabs (Ipswich ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-βIII tubulin was obtained from Promega (Cat. N° G712A) (Madison ...
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bioRxiv - Biochemistry 2020Quote: ... Asp-N (gold mass spectrometry grade) was purchased from Promega.
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bioRxiv - Biochemistry 2022Quote: ... Chymotrypsin (#V1062) and Asp-N (#V1621) were purchased from Promega. Bryostatin-1 (#2383 ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then digested using modified trypsin (Promega, P/N V5113) at a 1:20 (w/w ...
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bioRxiv - Microbiology 2021Quote: N-terminal HaloTag KIF4 expressing plasmid (pFN21ASDB3041) was purchased from Promega. The N-terminal Myc-tagged KIF4 (both wild type and ATPase-null motor inactive mutant ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... CMV-LgBit was made by amplifying LgBit from pBiT1.1-N (Promega) and ligating into pcDNA3.1 (+ ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 units of Peptide N-glycosidase F (PNGase F, Promega) enzymes according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... containing 500 μl of nuclease free water (Cat. N°. P119C/Promega/U.S.A). The upper part of the swabs was cut using sterile scissors to allow closing of the tube ...
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bioRxiv - Cancer Biology 2022Quote: ... and N-glycans were released following the addition of PNGase F (Promega) in PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... We also included N-terminal and C-terminal HiBiT tags (Promega, Madison, WI) for quantification using luminescence ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with NOCT N-terminal mutant constructs using Fugene HD (Promega) in a ratio of 3 µL transfection reagent per 1 µg DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... HiBiT fusion vectors were constructed using the parental vector pBiT3.1-N (N2361, Promega). To generate the HiBiT-VHL vector ...
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bioRxiv - Molecular Biology 2023Quote: ... NanoLuc fusion vectors were constructed using the parental vectors pNLF1-N (N1351, Promega) and pNLF1-C (N1361 ...