Labshake search
Citations for Promega :
1 - 50 of 410 citations for Gamma secretase subunit APH 1B APH1B Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... LgBiT subunit (Promega) was fused at the C-terminus of PTH2R connected by a 20-amino acid linker ...
-
bioRxiv - Molecular Biology 2022Quote: ... LgBiT subunit (Promega) was fused at the C terminus of RXFP4 connected by a 15-amino acid polypeptide linker ...
-
bioRxiv - Biochemistry 2021Quote: ... in which dissociation of a Gα subunit from a Gβγ subunit was monitored by a NanoBiT system (Promega). Specifically ...
-
bioRxiv - Molecular Biology 2022Quote: ... 50 ng/well of chaperone RTP1S,105 50 ng/well of the G protein subunit Gαolf,106–107 olfactory G protein subunit Gγ13,108 and 50 ng/well of pGloSensorTM-22F (Promega, Madison, USA).109 The utilized pGloSensor™-22F is a genetically engineered luciferase with a cAMP-binding pocket ...
-
bioRxiv - Biophysics 2024Quote: ... in which the interaction between a Gα subunit and a Gβγ subunit was monitored by the NanoBiT system (Promega). Specifically ...
-
bioRxiv - Microbiology 2024Quote: ... 500 IU/ml of human interferon-gamma (IFN-γ) for the gamma activated sequence reporter for 18h and lysates were prepared using Passive lysis buffer (Promega). Supernatants were collected and assayed for firefly and Renilla luciferase activities using the Dual luciferase reporter assay system (Promega E1960 ...
-
bioRxiv - Biochemistry 2022Quote: ... in which the interaction between the Gα subunit and the Gγ subunit was monitored by the NanoBiT-based enzyme complementation system (Promega). Specifically ...
-
bioRxiv - Biochemistry 2022Quote: ... the above PTH1R construct was added the LgBiT subunit (Promega) at the C terminus of PTH1R ...
-
bioRxiv - Biochemistry 2023Quote: ... the above PTH1R construct was added the LgBiT subunit (Promega) at the C terminus of PTH1R with a 17-amino acid linker ...
-
bioRxiv - Biochemistry 2022Quote: ... 1B and transferred to 37°C immediately after addition of supercoiled pGemT target DNA (Promega). Reactions were incubated for 30 ...
-
bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
-
bioRxiv - Biophysics 2021Quote: ... the NanoBiT tethering strategy was applied by fusing a LgBiT subunit (Promega) at the receptor C-terminus using homologous recombination (CloneExpress One Step Cloning Kit ...
-
bioRxiv - Microbiology 2024Quote: ... a gamma activated sequence firefly luciferase reporter plasmid (pGL4[luc2P/GAS RE Hygro] (Promega), and a constitutively expressing Renilla luciferase reporter plasmid pRL-TK (Promega).
-
bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity present in 10 µl of lysate clarified by centrifugation was then quantitated in white 96-well luminometer plate (Microlumat Plus 1b 96V instrument, Berthold Technologies) using 50 µl of luciferase assay reagent (Promega).
-
bioRxiv - Biophysics 2023Quote: ... the NF-κB subunit p65 (pci-Neo-p65 HaloTag)49 and HaloTag itself (pHTN, Promega).
-
bioRxiv - Biochemistry 2024Quote: ... bacmid DNA containing all subunits was transfected into Sf9 cells using FuGENE® HD (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... small leaves and witches’ broom) (Figure 1B) were collected for total genomic DNA extraction using the Wizard Genomic DNA Purification Kit (A1120; Promega, USA). For Illumina sequencing ...
-
bioRxiv - Biochemistry 2020Quote: ... bacmid DNA containing all four subunits was transfected into Sf9 cells using FuGENE® HD (Promega) per manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... We amplified the Cytochrome c oxidase subunit I (COI) for individual abdomen DNA using the GoTaq (Promega) protocol ...
-
bioRxiv - Immunology 2022Quote: ... The constructs encoding EROS/CYBC1 or gp91phox/CYBB fused to the reporter subunits were custom made by Promega and consist of the following ...
-
bioRxiv - Biochemistry 2022Quote: ... Rat Gβ1 with an N-terminal MHHHHHHSSGLVPRGSHMASHHHHHHHHHH-tag (His16) was fused with the SmBiT subunit (peptide 86, Promega)20 via a 15 amino acid (GSSGGGGSGGGGSSG ...
-
bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...
-
bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
-
bioRxiv - Neuroscience 2024Quote: ... then transfected with pRK5-based constructs of iGluR subunits and Neto variants (1 µg total DNA/ ml cells) using ViaFect transfection reagent (Promega). The cells were incubated at 37°C for 3 hours ...
-
bioRxiv - Physiology 2022Quote: ... 1.15 μg of purified AQP2 or AQP2 S256A COOH-tail peptide were mixed with either RSK (16 nmol) or PKA catalytic subunit (5.6 nmol) (Promega, Madison, WI) in a total volume of 12 μl reaction buffer containing 40 mM Tris pH 7.5 ...
-
bioRxiv - Cell Biology 2021Quote: The medium in 96-well plate was replaced at the indicated time points with 100 μl of PBS supplemented with 0.2 g/l EDTA and 50 μM FITC-VAD-fmk (Promega, USA) or 50 μM 1,1’,3,3,3’,3’-hexamethylindodicarbo-cyanine iodide (DiIC1(5) ...
-
bioRxiv - Cell Biology 2022Quote: ... were stained with CaspACE FITC-VAD-FMK (fluorescein isothiocyanate-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone) in situ Marker (Promega, Mannheim, Germany) as per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: The increase in caspase like enzymatic activity was evaluated by using a staining solution containing (FITC)-VAD-FMK (CaspACE™, Promega) at a final concentration of 10 µM/ml (74) ...
-
bioRxiv - Immunology 2024Quote: ... Cell viability was assessed by incubating at 37°C up to 107 cells resuspended in complete RPMI medium containing 10 µM of the CaspACE™ FITC-VAD-FMK in Situ Marker (Promega). After 30 min ...
-
bioRxiv - Cell Biology 2021Quote: ... LgBiT antibody (N710A) and HiBiT antibody were acquired from Promega. Chicken polyclonal MAP2 (ab5392) ...
-
bioRxiv - Molecular Biology 2021Quote: ... antibodies were detected using HRP-conjugated mouse secondary antibody (Promega). UAP56/DDX39B [1:2000] (custom generated51 ...
-
bioRxiv - Molecular Biology 2021Quote: ... antibody was detected using HRP-conjugated chicken secondary antibody (Promega).
-
bioRxiv - Molecular Biology 2021Quote: ... antibodies were detected using HRP-conjugated rabbit secondary antibody (Promega). Anti-Beta Tubulin III (Tuj1 ...
-
bioRxiv - Immunology 2023Quote: ... Secondary antibodies (Promega) were incubated for 1 h at RT ...
-
bioRxiv - Microbiology 2020Quote: ... antibody with HRP-conjugated anti-mouse secondary antibody (GE Healthcare or Promega). In addition to the experimental samples ...
-
bioRxiv - Plant Biology 2020Quote: ... an immunoblot with anti-halo antibody (Anti-HaloTag® Monoclonal Antibody-Promega) was performed to confirm protein expression and binding efficiency for each TF ...
-
bioRxiv - Microbiology 2023Quote: ... Primary antibodies used are anti-HaloTag monoclonal antibody (Promega, G921A, 1:1000), and polyclonal rabbit antibodies ...
-
bioRxiv - Microbiology 2024Quote: ... as primary antibody and an anti-rabbit IgG HRP-conjugated antibody (Promega) as secondary antibody ...
-
bioRxiv - Immunology 2023Quote: ... Anti-HiBiT antibody (Promega) was used as a positive control for each peptide ...
-
bioRxiv - Biophysics 2023Quote: ... The secondary antibody (Promega: anti-rabbit #W4011 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Secondary Antibody (Promega PRW4011), and 1-Step™ Ultra TMB-Blotting Solution (Invitrogen™ 37574 ...
-
bioRxiv - Biochemistry 2022Quote: ... but the secondary antibodies were goat anti-mouse HRP conjugated antibody (Promega Cat #W4021) and goat anti-rabbit HRP conjugated antibody (Promega Cat # W4011) ...
-
bioRxiv - Microbiology 2023Quote: ... Antibodies were visualized by using anti-rabbit immunoglobulin G-alkaline phosphatase secondary antibodies (Promega) and the CDP-star detection system (Roche Diagnostics ...
-
bioRxiv - Genetics 2020Quote: ... Secondary antibodies were from Promega (catalog nos ...
-
bioRxiv - Molecular Biology 2020Quote: ... HRP Conjugate Antibody (Promega-W4021). Immuno-blots were developed using enhanced chemiluminescence (Clarity -Biorad ...
-
bioRxiv - Molecular Biology 2020Quote: ... HRP Conjugate antibody (Promega -W4011), Anti-Mouse IgG (H+L) ...
-
bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
-
bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used included alkaline phosphatase-conjugated anti-rabbit IgG antibody (1:1,000, #S3738, Promega) and anti- mouse IgG antibody (1:1,000 ...
-
bioRxiv - Cell Biology 2023Quote: ... Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000, Promega). Chemiluminiscent signals were detected with an ECL Prime Western blotting detection kit (GE Healthcare ...
-
bioRxiv - Plant Biology 2022Quote: ... for 2 h as primary antibody and peroxidase-conjugated goat anti-rabbit antibody (1:10,000; Promega) for 1 h with 5 times 10 min washes in-between the incubations ...