Labshake search
Citations for Promega :
1 - 50 of 334 citations for Galectin 9 Human HEK293 GST since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
-
bioRxiv - Plant Biology 2020Quote: ... GST or GST-OsDMI3 immobilized on the Magnet GST Particles (Promega) were incubated with His-OsMKK1/OsMKK6 in GST binding buffer (2 mM KH2PO4 ...
-
bioRxiv - Molecular Biology 2022Quote: ... we amplified FES exons 9 to 11 with intervening sequences and SH3BP2 exons 9 to 11 with intervening sequences from human genomic DNA (Promega), and cloned the products into pcDNA3.1(+ ...
-
bioRxiv - Developmental Biology 2021Quote: ... the GST tag was cleaved from GST-TES-1 using ProTEV Plus (Promega), according to manufacturer’s instructions.
-
bioRxiv - Biochemistry 2023Quote: ... recombinant GST-tagged MARK2 (Promega) was incubated with tau at 30 °C at 1:100 ratio of MARK2:tau in phosphorylation buffer (25 mM PIPES ...
-
bioRxiv - Molecular Biology 2020Quote: ... Human NOCT (1-431) or Schistosoma japonicum GST coding sequences were inserted into pFC3F using the Flexi Cloning System (Promega). To generate NOCT Δ(2-15)-3F ...
-
bioRxiv - Cell Biology 2023Quote: HaloTag-GST (61 kDa) (Promega, G4491) was labelled with JF646 for 4 hours at 4 ° C on ice with 3x excess HaloTag ligand (buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with Galectin-3-GFP plasmid (0.5 µg) using FuGene HD (Promega, Madison, WI) with a ratio of 3:1 for 24 h at 37 °C in 5% CO2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... the GST region of psfRps29-GST was replaced with luciferase cDNA (pNL1.1[Nluc]Vector; Promega Madison, WI, USA). Luciferase cDNA was amplified via PCR using primers Nluc_FW2 and Nluc_RV2 ...
-
bioRxiv - Immunology 2023Quote: ... or 9 Reagent (Promega) with MG-132 Inhibitor (Promega ...
-
A rare variant on a common risk haplotype of HFE causes increased risk of hereditary hemochromatosisbioRxiv - Genetics 2019Quote: ... or HEK293 cells using Fugene 6 (Promega) following manufacturer’s instructions and studied 48-72 hours post-transfection.
-
bioRxiv - Developmental Biology 2022Quote: ... and Klf4 vectors were co-transfected with pCL-Eco (Addgene ID 12371)149 in HEK293 cells with FuGENE6 (Promega) using low volume transfection protocol (Steffen et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells grown on 10cm culture dishes were transiently transfected with the different HA-GST-RagC mutants and wildtype HA-GST-RagA using Fugene HD (#E2311, Promega). The following day ...
-
bioRxiv - Microbiology 2023Quote: 1 ml of recombinant GST or GST-YgfB protein at a concentration of 10 μM was incubated with 100 μl 50 % MagneGST (Promega) bead-slurry equilibrated with pulldown-buffer for 45 min at 4°C and washed two times with 500 μl pulldown-buffer ...
-
bioRxiv - Plant Biology 2023Quote: ... EIL1-GST and GST proteins were synthesized using the TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega, L3260), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were transfected with Fugene 6 (Promega). All other cell lines were transfected using Lipofectamine 2000 (ThermoFisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... into HEK293 using FuGENE 6 transfection reagent (Promega). Culture supernatant containing lentiviral particles was harvested after 24-48h incubation and passed through a 0.45 μm filter ...
-
bioRxiv - Molecular Biology 2021Quote: ... we used Magne-GST Pull-Down Systems (Promega, USA). The bait ...
-
bioRxiv - Biochemistry 2021Quote: ... GST-4xSUMO2 was purified using MagneGST Glutathione particles (Promega) as previously described150 ...
-
bioRxiv - Plant Biology 2020Quote: ... GST-Halo was used as a protein standard (Promega). Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel ...
-
bioRxiv - Cell Biology 2020Quote: HEK293 autophagy reporter cells (HiBiT-HaloTag-LC3, Promega #GA1040) were grown in in DMEM (ThermoFisher #31053-028 ...
-
bioRxiv - Cell Biology 2020Quote: ... HEK293 were transiently transfected using Fugene HD (Promega, U.K.) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... HEK293 cells were transiently transfected using Fugene 6 (Promega) with GFP-Tnf 3’UTR reporter constructs and a pGL3-mCherry control construct ...
-
bioRxiv - Systems Biology 2022Quote: ... 9 μg Transfection Carrier DNA (Promega) and 30 μL FuGENE HD Transfection Reagent (Promega ...
-
bioRxiv - Immunology 2022Quote: ... Ectopic Flag-tagged Galectin 8 or HA-tagged LILRB4 plasmid were transfected into the Mc38/B16/HEK294 cells with Fugene (Promega). A blank vector control was applied ...
-
ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... HaloTag-GST was used as a negative control antigen (Promega).
-
bioRxiv - Molecular Biology 2022Quote: HEK293 cells cotransfected with pFN21A HaloTag CMV Flexi vector (Promega) expressing PALLD and pNLF1-N [CMV Hygro] or pNLF1-C [CMV Hygro] vector (Promaga ...
-
bioRxiv - Cell Biology 2021Quote: HEK293 stably expressing pcDNA3.1(+)_SSF-GCGR cells transfected with GloSensor20F (Promega) were lifted and resuspended in imaging media (DMEM without phenol red supplemented with 30 mM HEPES ...
-
bioRxiv - Systems Biology 2021Quote: ... HEK293 cells were co-transfected using Fugene 6 transfection reagent (Promega) with MAC-tag (600ng ...
-
bioRxiv - Cell Biology 2019Quote: ... and 9 μl Fugene HD transfection reagent (Promega) was incubated for 10 minutes at room temperature and transfected into each well ...
-
bioRxiv - Cell Biology 2019Quote: ... and 9 µl Fugene HD transfection reagent (Promega) was incubated for 10 minutes at room temperature and transfected into each well ...
-
bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removed from hTNPO1 using proTEV Plus Protease (Promega), and the protease enzyme was further removed from hTNPO1 protein by Ni-NTA Magnetic Beads (NEB ...
-
bioRxiv - Cell Biology 2022Quote: GST-AIMP1 was in-gel digested with Trypsin GOLD (Promega, Madison, WI). Tryptic peptides were separated by reversed-phase chromatography using Eazynano LC II (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... GST-dimerized dynein constructs were labeled with 5 mM Halo-TMR (Promega) in the column for 10 min at room temperature and the unbound dye was washed away with TEV buffer at 4 °C ...
-
bioRxiv - Molecular Biology 2020Quote: HEK293 cells were rinsed and lysed with Passive Lysis Buffer (PBL, Promega), protein concentrations determined using the DC protein assay (Bio-Rad) ...
-
bioRxiv - Developmental Biology 2021Quote: ... retroviral plasmid was transfected to HEK293 cells using FuGENE 6 (Promega, E2692). Two days after transfection ...
-
bioRxiv - Molecular Biology 2022Quote: HCT116 cells were transfected using JetPrime(Polyplus) and HEK293 using Fugene (Promega) using a standard protocol ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasmid DNAs were transfected into HEK293 cells with ViaFect Transfection Reagent (Promega) according to manufacturer’s instruction.
-
bioRxiv - Physiology 2023Quote: ... whereas HEK293 cells stably expressing a luminescent cAMP GloSensor (GS-293) (Promega) were previously created in our lab 25 ...
-
bioRxiv - Biochemistry 2021Quote: ... 9 by adding 20 μl CellTiter-Blue reagent (Promega) to wells and measuring luminescent signal using GloMax discover microplate reader (Promega).
-
bioRxiv - Cancer Biology 2021Quote: ... and luminescence-based caspase-glo 9 assay system (Promega), respectively ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... consisting of 9 µg/mL of carrier DNA (Promega) and 1 µg/mL of CSNK2A-NLuc fusion DNA in Opti-MEM without serum (Gibco) ...
-
bioRxiv - Cell Biology 2024Quote: ... consisting of 9 µg/mL of carrier DNA (Promega) and 1 µg/mL of CSNK2A2-NLuc fusion DNA in Opti-MEM without serum (Gibco) ...
-
bioRxiv - Cell Biology 2024Quote: ... and GST-C-WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega).
-
bioRxiv - Neuroscience 2019Quote: ... HEK293 cells were transfected in the 24 well plates using Fugene HD (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Retrovirus was produced through transfection of HEK293 cells using FuGENE 6 (Promega, E2692). Two days after transfection ...
-
bioRxiv - Molecular Biology 2024Quote: ... 25,000 HEK293-T cells were seeded and transfected with FuGENE 6 (#E231A, Promega) and plasmid DNA at the carrier (µL):DNA (µg ...
-
bioRxiv - Cancer Biology 2021Quote: ... 50μl of either caspase-8 reagent or caspase-9 (Promega) reagent was then added with MG132 proteosome inhibitor ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... was prepared with 9 μg/mL of Carrier DNA (Promega) and 1 μg/mL of NL-BRSK2 to achieve a total volume of 1.05 mL ...
-
bioRxiv - Microbiology 2023Quote: ... and random 9-mers in the presence of RNasin (Promega). qPCR was then performed on a Quantstudio 3 using the indicated targets and with iTaq Universal SYBR Green (BioRad) ...