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Citations for Promega :
1 - 50 of 1580 citations for GATOR2 complex protein WDR59 WDR59 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Systems Biology 2023Quote: ... 0.5ul “home-made” Tn5 transposase adapter complex and 0.4mg/ml digitonin (Promega, #G9441) at 30 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 11S protein was detected with a rabbit polyclonal antibody from Promega, and Tom40 with a rabbit polyclonal antibody produced by Cambridge Research Biochemicals (Billingham ...
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bioRxiv - Immunology 2024Quote: Recombinant antibodies were purified using protein G magnetic beads (Promega #G7472), HiTrap protein G HP columns (Cytiva #17040401) ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were detected with secondary peroxidase-conjugated antibodies (Promega, Madison, WI, USA) and ECL ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... smegmatis Complex I (8 μg) was digested with MS-grade trypsin/Lys-C mix (Promega) at a 25:1 (w/w Complex I:protease ratio in 25 mM Tris-HCl ...
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bioRxiv - Bioengineering 2024Quote: ... A complex of 1 μg of plasmid was mixed with 3 μl of Viafect (Promega) in OPTI-MEM and mixed and incubated at room temperature for 20 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... Protein production was confirmed by western blot using an anti-HaloTag antibody (Promega). HaloTag-ligand conjugated magnetic beads (Promega ...
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bioRxiv - Immunology 2020Quote: ... The IKKα/β kinase activity in the IP complex was tested by using IKKβ Kinase Enzyme Kit (Promega) and following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... 200 nM of biotinylated Arr3_ýC·IP6 complex was immobilized on 250 µl Streptavidin magnetic beads (Promega, Cat No: Z5482) and incubated with 100 μl of a phage library E (Miller et al. ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged or HA-tagged protein was detected using a commercially available mouse monoclonal antibody (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured for 24 h with a complex of DNA and FuGENE® HD Transfection Reagent (#E2311; Promega) at a ratio of 1:3 prepared in Opti-MEM reduced serum medium (#31985070 ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of plasmid DNA encoding for SthCsm-complex and guide RNA was mixed with 1.5 μL of FuGENE HD reagent (Promega), incubated for 15 min ...
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bioRxiv - Systems Biology 2023Quote: Methyltransferase activity of the NSP10-NSP16 complex and inhibitor screening was performed using the MTase-Glo assay (Cat. No. V7601, Promega) (Hsiao et al ...
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bioRxiv - Microbiology 2023Quote: The glycosyltransferase activity of the CslA-GlxA complex was quantitatively measured by using the UDP-GloTM glycosyltransferase assay kit (Promega). This assay detects the content of free UDP ...
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bioRxiv - Plant Biology 2022Quote: ... Protein expression was confirmed by Western blot with Anti-Halo Tag monoclonal antibody (1:2000) (G9211, Promega).
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bioRxiv - Cell Biology 2023Quote: Cells grown under confluency were incubated in CM+bFGF media containing 1μM cell permeable Janelia Fluor® 549 HaloTag ligand complex (Promega, GA1110) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... transfected with luciferase reporter constructs or containing the relevant promoter regions using FuGENE HD Transfection Reagent Complex (Promega, Madison, Wisconsin, USA). pCMV-NFIA1.1 (a kind gift from Richard Gronostajski ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
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bioRxiv - Molecular Biology 2023Quote: ... The A7r5 cells and iPSC-SMCs (from 31.3 iPSC clone) were transfected respectively using FuGENE HD Transfection Reagent Complex (Promega, Madison, Wisconsin, USA) and Amaxa Basic Nucleofector kit for primary mammalian SMCs (LONZA ...
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bioRxiv - Immunology 2021Quote: ... The antibodies were purified from the culture supernatant 4 – 6 days later using protein G magnetic beads (Promega #G7472). Purified antibodies and antibody elution buffer (5 parts elution buffer (100 µM glycine-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Proteins separated on the second gel were transferred to a PVDF membrane and DivJ levels were measured using Western blots by probing the protein with anti-HaloTag antibody (Promega). Due to the requirement of growing cells in low phosphate media ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were transferred by semi-dry Western Blot onto PVDF membranes visualised with polyclonal antibody and BCIP/NBT (Promega, S3771).
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bioRxiv - Molecular Biology 2022Quote: ... Expression of Halo-bZIP and SBP-bZIP proteins were confirmed by western blotting using anti-HaloTag monoclonal antibody (Promega, G9211) and anti-SBPTag mouse anti-human antibody (MilliporeSigma ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of FgSte3-NLuc donor protein produced was also validated performing immunoblotting using anti-Nluc antibody (Promega cat#7000), see details below.
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bioRxiv - Immunology 2024Quote: ... 2 µg of anti-DNA-PKcs antibody or the corresponding IgG control was incubated with 70 ng of recombinant DNA-PKcs protein (Promega) in 500 µL of lysis buffer (20 mM Tris–HCl [pH 7.4] ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then digested with LysC at 1ug/100ug protein (Promega) for 3 h at room temperature (RT ...
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bioRxiv - Plant Biology 2020Quote: ... Fusion proteins were purified using MagnetHis™ protein purification system (Promega) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... Proteins were precipitated through the addition of Protein Precipitation Solution (Promega) followed by vigorous vortexing ...
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bioRxiv - Biochemistry 2022Quote: ... Mass Spec-Compatible Human Protein Extracts, Cat# V6951) and digested yeast protein extract (Mass Spec-Compatible Yeast Protein Extracts, Cat# V7461) were from Promega, commercial human plasma samples (Human Source Plasma ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were purified by using MagneHis™ Protein Purification System (Promega, USA). The peptides of the transporters PDR6 ...
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bioRxiv - Biophysics 2022Quote: ... Fusion protein was purified using HisLink Protein purification resin (Promega (Madison, WI) #V8821 ...
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bioRxiv - Molecular Biology 2021Quote: ... A protein standard (Promega) was added to the non-tagged cells as a known reference of protein concentration to luminescence output ...
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bioRxiv - Microbiology 2022Quote: ... Protein Precipitation Solution (Promega) was added to the lysate ...
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bioRxiv - Molecular Biology 2020Quote: ... HaloTag Standard Protein (Promega) was labelled with 10 fold molar excess (5 µM ...
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bioRxiv - Genetics 2023Quote: ... Protein Precipitation Solution (Promega) was added to the lysed mixture ...
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bioRxiv - Microbiology 2023Quote: ... Protein precipitation solution (Promega) was added and samples were vigorously mixed ...
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bioRxiv - Microbiology 2024Quote: ... Protein Precipitation Solution (Promega) was added to remove proteins from the solutions ...
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bioRxiv - Neuroscience 2023Quote: Protein-protein interaction was analyzed using the NanoBit Protein:Protein Interaction (PPI) System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were expressed with TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega) and reactions were incubated for 12h at 25°C with 10µg pIX-HALO expression clone ...
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bioRxiv - Plant Biology 2023Quote: ... and the total protein contents were quantified using BCA Protein Assay Reagent (Promega Corporation). The supernatants were stored at –80 ℃ until subsequent analysis.
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were digested with trypsin/Lys-C mix (1:25 protease: protein; Promega) at 37°C was performed with constant mixing using a thermomixer ...
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bioRxiv - Genomics 2022Quote: ... and the LgBiT Protein (Promega) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Magne Protein G beads (Promega) were used to purify the antibodies according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... In vitro translated proteins (Promega SP6-TNT Quick rabbit reticulocyte lysate system ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were trypsinized (Promega) in 40mM ammonium bicarbonate (FISHER SCIENTIFIC) ...
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bioRxiv - Cancer Biology 2022Quote: ... A BCA protein assay (Promega) was used to determine protein concentration ...