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Citations for Promega :
101 - 150 of 3715 citations for G Protein Signaling Modulator 1 GPSM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... appropriate secondary antibody (1:10.000) coupled to alkaline phosphatase (Promega) was added and incubated for one hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies include anti-NLuc rabbit polyclonal (1:6,000) (Promega), anti-mCherry rabbit polyclonal (1:5,000 ...
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bioRxiv - Immunology 2022Quote: ... HRP conjugated secondary antibodies were purchased from Promega (1:5000) or Novus (1:5000).
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bioRxiv - Cell Biology 2022Quote: ... Anti-Halo antibody was purchased from Promega (G9211; 1:1000). Anti-actin-HRP antibody (C4 ...
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bioRxiv - Immunology 2022Quote: ... and a non-target control (NT-Ctrl) along with the packaging plasmids (pHCMV-G, and pHCMV-HIV-1) (44) using FUGENE-HD transfection reagent (Promega). The mouse Drp1-targeted shRNA plasmid with the sense sequence of GGCAATTGAGCTAGCTATA ...
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bioRxiv - Biochemistry 2023Quote: ... 1.5 µg of the pAC8RedNK vector and 0.5 µg of linearized baculoviral DNA were concurrently transfected into 1×106 Sf9 cells using 6 µl of FuGene HD (Promega) in ESF 921 Insect Cell Culture medium (Expression Systems) ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested in 1 M urea with modified trypsin (Promega, Madison, WI) at a 1:50 enzyme-to-substrate ratio ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were digested at 37 °C overnight with trypsin (Promega; 1:10, enzyme/substrate) in the presence of 10% acetonitrile ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were digested overnight at 37 °C using trypsin (1:50, w/w, Promega). Peptides were extracted in ACN and formic acid and combined extracts were freeze-dried ...
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bioRxiv - Developmental Biology 2021Quote: ... 50 μl of 0.5M TEAB and trypsin (Promega, enzyme: protein ratio of 1:50) were added to the membrane ...
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bioRxiv - Microbiology 2021Quote: ... and digested with sequencing grade trypsin (Promega, 1:50 trypsin- to-protein [wt/wt]) (Yang et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Protein enzymatic cleavage was carried out with trypsin (1:20, w/w; Promega, V5280) at 37 °C for 16 h ...
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bioRxiv - Molecular Biology 2022Quote: ... and the proteins were digested with 1/100 (w/w) trypsin (Promega, Cat # V5111) overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: ... with a 1:50 ratio of purified protein to Endoglycosidase H (Endo H) (Promega). After treatment ...
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were 1:5,000 HRP-conjugated anti-rabbit (W4011; Promega) or mouse (W4021 ...
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bioRxiv - Biochemistry 2019Quote: ... and HRP-conjugated rabbit anti-mouse antibody 1:10,000 (Promega, W4028). The plate was developed with 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid ...
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bioRxiv - Neuroscience 2021Quote: ... JF-549 or Halo-tag antibody (1:500, Promega, mouse monoclonal) and measuring the newly synthesized actin (in most cases ...
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bioRxiv - Neuroscience 2021Quote: ... Immunofluorescence utilizing primary antibodies directed against βIII-tubulin (1:1000, Promega) and secondary antibodies Alexa Fluor-488 (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... Washes and incubation of HRP-conjugated secondary antibodies (1:5000, Promega) were performed in PBST (0.05% Tween 20 in PBS) ...
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bioRxiv - Genetics 2020Quote: ... Alkaline phosphatase-conjugated secondary antibodies were used at 1:10000 (Promega). Western blot band intensity was analyzed with ImageJ Gel quantification tool.
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used were anti-p75NTR (Promega, Cat: G323A, 1:300) and anti-GAPDH (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used: HaloTag mAB (Promega, G9211, 1:500), GAPDH (Cell Signaling 2118 ...
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bioRxiv - Biochemistry 2023Quote: ... The following primary antibodies were used: NanoLuc (N7000, Promega, 1:1000), β-ACTIN (#4970S ...
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bioRxiv - Plant Biology 2024Quote: ... and HRP-conjugated secondary antibody (Promega, Cat# W4011, 1:4,000 dilution).
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bioRxiv - Plant Biology 2019Quote: ... An aliquot containing 4 μg RNA was treated for 45 min with 2 μl of DNase RQ1 (1 μg/μl) at 37 °C (Promega, UK), and purified using an RNAeasy spin column (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... Phospho-Tyrosine specific antibodies (anti P-Tyr674/675 from Cell Signalling 1:3000) and anti-p75 intracellular antibody (Promega) were used ...
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bioRxiv - Microbiology 2019Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega) also used at a 1:10,000 dilution.
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bioRxiv - Genetics 2022Quote: ... membranes were washed and incubated with the appropriate HRP conjugated secondary antibody (1:3,000 anti-rabbit W401B antibody from Promega), and the reaction was finally visualized with the Western Blotting Luminol Reagent (Santa Cruz Biotechnology) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein expression was confirmed by Western blot with Anti-HaloTag monoclonal Ab (1:2000) (Promega). Cy3-labeled and unlabeled dsDNA probes were generated with oligonucleotides listed in Supplemental Table 5 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins in each well were digested with 1 μg of sequencing-grade trypsin (Promega Inc.) in 75 μl of 50 mM ammonium bicarbonate and incubated overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... On-bead protein digestion was performed by adding 1 µg of sequencing grade trypsin (Promega) to the samples ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested on-bead either with sequencing-grade trypsin (1:50) (Promega, Walldorf, Germany) for 4 h at 800 rpm and 42°C or thermolysine (1:50 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins bound to beads were digested with 1 μg of Trypsin/Lys-C mix (Promega) for 3 h at 37 °C and then supplemented with and additional amount of 150 ng Trypsin/Lys-C for overnight digestion on an end-over-end rotation apparatus at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C by addition of 1 µg of trypsin (Promega). Thereafter ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were in-gel digested with trypsin (Promega, Madison, WI, USA; ratio 1:100) at 37°C overnight after de-staining ...
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bioRxiv - Molecular Biology 2021Quote: ... chymotrypsin in a chymotrypsin/protein ratio of 1/20 (w/w) (Promega Cat. No. V1061) and incubated overnight at 25 °C or endoproteinase GluC in a GluC/protein ratio of 1/20 (w/w ...
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bioRxiv - Developmental Biology 2022Quote: ... The 50μg of proteins were then trypsin digested overnight at 37 °C (1:25; Promega) using the filter aided sample preparation (FASP ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were extracted by methanol-chloroform precipitation and digested with 1 μg of trypsin (Promega) in 100 mM EPPS (pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... followed by overnight digestion with trypsin (enzyme to protein ratio of 1:100) (Promega, Germany) at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 37° C with 1 μg mass spectrometry grade Trypsin (Promega). The resulting peptide sample was acidified with 10% formic acid and 10% trifluoroacetic acid (TFA ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples eluted with biotin were digested in-solution with 1 ug Trypsin/LysC (Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Proteins separated on the second gel were transferred to a PVDF membrane and DivJ levels were measured using Western blots by probing the protein with anti-HaloTag antibody (Promega). Due to the requirement of growing cells in low phosphate media ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were transferred by semi-dry Western Blot onto PVDF membranes visualised with polyclonal antibody and BCIP/NBT (Promega, S3771).
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bioRxiv - Molecular Biology 2022Quote: ... Expression of Halo-bZIP and SBP-bZIP proteins were confirmed by western blotting using anti-HaloTag monoclonal antibody (Promega, G9211) and anti-SBPTag mouse anti-human antibody (MilliporeSigma ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of FgSte3-NLuc donor protein produced was also validated performing immunoblotting using anti-Nluc antibody (Promega cat#7000), see details below.
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bioRxiv - Cancer Biology 2021Quote: Trypsin digestion was carried out overnight at 37 °C with 1:50-1:100 enzyme– protein ratio of sequencing grade-modified trypsin (Promega V5111) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... and 0.2 g Renilla plasmid (pRL-CMV, Promega, E2261) at room temperature ...