Labshake search
Citations for Promega :
251 - 300 of 487 citations for FabFc ZAP Hug M since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... or UAS-V5-Ten-m-ΔECD-FLAG constructs using the FuGENE HD transfection Reagent (Promega). After 48 hours ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... cDNA was synthesized from 2 μL of the eluted RNA using M-MLV reverse transcriptase (Promega) and random hexamer primers ...
-
bioRxiv - Microbiology 2021Quote: ... and was reverse transcribed into cDNAs using oligo-d(T) and M-MLV reverse transcriptase (Promega). Real-time PCR was performed with the specific primers listed in Table 2 using the LightCycler 480 (Roche ...
-
bioRxiv - Molecular Biology 2020Quote: ... The samples were dissolved in 0.1 M ammonium bicarbonate and digested with sequencing grade trypsin (Promega) at a protease/protein ratio of 1:50 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1.33 μL 1 M Tris-HCL pH 8.5 and 7.8 μL 0.5 mg/mL trypsin (Promega) were added and proteins left to digest for 16 hours at 37°C ...
-
bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized from 0.5 µg total RNA with M-MLV Reverse Transcriptase (Promega). All qRT-PCR analyses were performed by CFX96 real-time PCR detection system with iQ SYBR green supermix (Bio-Rad) ...
-
bioRxiv - Plant Biology 2020Quote: ... 2 μg RNA was used for cDNA synthesis with M-MLV Reverse Transcriptase (Promega, Madison, USA) and 1 μL of the obtained cDNA was used for real-time PCR with SYBR™ green master mix (Bioline ...
-
bioRxiv - Microbiology 2020Quote: ... The lysate was then diluted to <2 M urea and incubated with 50 μg trypsin (Promega) overnight at 37 °C under agitation ...
-
bioRxiv - Microbiology 2020Quote: ... Urea was diluted to 2 M with 100 mM Tris-HCl pH 7.0 and trypsin (Promega) was added at a mass ratio of 1:100 (trypsin:protein) ...
-
bioRxiv - Biochemistry 2022Quote: ... Ethylenediaminetetra-acetic acid (EDTA, 0.5 M, pH 8.0) and trypsin were purchased from Promega (Madison, WI). Lysyl endopeptidase (Lys-C ...
-
bioRxiv - Neuroscience 2020Quote: ... equal amounts of total RNA were transformed by oligo(dT) and M-MLV reverse transcriptase (Promega). Ten nanograms of cDNA were amplified in the Applied Biosystems 7500 Fast Real Time PCR System thermocycler (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... 500-1500ng of extracted RNA were used for each RT reaction using either M-MLV (Promega) or RevertAid (ThermoFisher ...
-
bioRxiv - Immunology 2020Quote: ... Complementary DNA (cDNA) was synthesized from total RNA (500 ng) using M-MLV Reverse Transcriptase (Promega) as per the supplier’s protocol (1 hr ...
-
bioRxiv - Neuroscience 2021Quote: ... Reverse transcription of 2 μg RNA was performed using M-MLV reverse transcriptase (Promega, WI, USA). The PCR-based amplification was done using synthesized-cDNA ...
-
bioRxiv - Molecular Biology 2023Quote: 500 ng of total RNA were converted to cDNA using M-MLV Reverse Transcriptase (Promega, M1705) and Oligo(dt ...
-
bioRxiv - Microbiology 2022Quote: ... The solution was diluted to below 1 M urea and digested using sequencing grade Trypsin (Promega). The proteolytic peptides were finally desalted using an Oasis HLB solid phase extraction well plate (Waters ...
-
bioRxiv - Systems Biology 2022Quote: ... The proteins were diluted to 0.1 M ammonium bicarbonate and digested overnight with Trypsin (Promega, V5113) at 37°C with a protein-to-enzyme ratio of 50:1 ...
-
bioRxiv - Immunology 2023Quote: ... The cDNA was synthesized from 2μl of the eluted RNA using M-MLV reverse transcriptase (Promega) and random hexamer primers ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA (1.2 µg) was utilized to reverse transcript with M-MLV Reverse Transcriptase kit (M1701, Promega), Random Hexamer Primers (48190011 ...
-
bioRxiv - Molecular Biology 2023Quote: ... First-strand cDNA synthesis was performed from 1 µg RNA using M-MLV Reverse Transcriptase (Promega) and random hexamer primers (Sigma-Aldrich ...
-
Deciphering the polycistronic nature of Mycobacterium tuberculosis lipoproteins of unknown functionsbioRxiv - Microbiology 2023Quote: ... The cDNA synthesis was performed using 1 μg of RNA with M-MLV reverse transciptase (Promega) (RT+ ...
-
bioRxiv - Cancer Biology 2023Quote: ... The total RNA was next reverse-transcribed to cDNA by M-MLV reverse transcriptase (Promega, M1701) 0.5 mg/mL Oligo(dT)15 primers were added to 200 ng of RNA and was incubated at 70°C for 5 mins ...
-
bioRxiv - Plant Biology 2023Quote: ... Four microgram of extracted RNA sample was reverse transcribed using the M-MLV Reverse Transcriptase (Promega) from each RNA sample ...
-
bioRxiv - Cancer Biology 2023Quote: ... Digests were diluted further to a 1.5 M urea concentration and 5 µg of trypsin (Promega) was added for 6 hours at 37°C ...
-
bioRxiv - Physiology 2024Quote: ... Reverse transcription was performed using oligo(dT) primers and M-MLV Reverse Transcriptase (Promega, Madison, WI) at 42°C for 1 hour according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... 0.1-1 µg of RNA was reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega) and oligo(dT ...
-
bioRxiv - Microbiology 2024Quote: ... trypsin digestion was performed after diluting urea to <1.2 M and using 0.5 µg trypsin (Promega), and processed peptides were dissolved in 20 µl of buffer A ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to 1 M urea with 50 mM ambic and digested with Trypsin (Promega #V5111) for 10 hours at 1:125 enzyme:protein at 37°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription was performed with random hexamer primers (R6) and M-MLV reverse transcriptase (Promega, Madison, WI) according to the manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... cDNA was generated with the DNase-treated RNA (5μg) and M-MLV reverse transcriptase (Promega, Madison, WI). The cDNA was then used as a template for qRT-PCR to determine relative gene expressions of TuCPR in different life stages of the susceptible T ...
-
bioRxiv - Biochemistry 2020Quote: ... The reverse transcriptase M-MLV RT as well as the T4 DNA ligase were obtained from Promega. The pGEM-T Vector kit was purchased from Promega ...
-
bioRxiv - Plant Biology 2020Quote: ... We synthesized cDNA using 2.0 μg of total RNA per sample and M-MLV Reverse Transcriptase (Promega) and poly-T primers (Sigma-Aldrich).
-
bioRxiv - Plant Biology 2021Quote: ... 100 ng of the recovered RNA was used as a template for M-MLV reverse transcriptase (Promega). The PCR reactions were performed in a T-ADVANCED S96G (Biometra ...
-
bioRxiv - Immunology 2021Quote: ... and reversed transcribed with random hexamers (IDT) using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega) according to the manufacturers’ instructions ...
-
bioRxiv - Immunology 2020Quote: RNA was extracted from ear homogenates and reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega). Real-time PCR was performed on an ABI PRISM 7500 sequence detection system (Applied Biosystems ...
-
bioRxiv - Microbiology 2019Quote: ... 1 μg of total RNA template was reverse transcribed to cDNA by M-MLV reverse transcriptase (Promega) using specific stem-loop RT primer and under the following conditions ...
-
bioRxiv - Physiology 2021Quote: ... Complementary DNA (cDNA) was synthesized from total RNA (300 ng) using M-MLV Reverse Transcriptase (Promega, #M1701) as per supplier’s protocol (1hr at 37°C followed by 10 min at 70°C) ...
-
bioRxiv - Physiology 2021Quote: ... Subsequent first strand synthesis of cDNA was conducted following the protocol of M-MLV reverse transcriptase (Promega). qPCR was conducted with SYBR-Green reagent and analyzed with the Quantstudio Real-Time PCR System ...
-
bioRxiv - Biochemistry 2022Quote: ... urea was diluted to 1 M by adding 50 mM ammonium bicarbonate and 2 μg Trypsin (Promega) per 100 μg protein ...
-
bioRxiv - Genomics 2022Quote: ... Reverse transcription was carried out using moloney-murine leukemia virus (M-MLV) reverse transcriptase kit (Promega, USA). Reverse transcription was completed in a 30 µL final volume reaction containing 400 U M-MLV and 1 mM dNTP mix (Invitrogen ...
-
bioRxiv - Genetics 2022Quote: Total RNAs were reversed into first-strand cDNA using the M-MLVRT reverse transcription kit (Promega, USA). qRT-PCR was performed with SYBR Premix Ex Taq (TaKaRa Biotech Co. ...
-
bioRxiv - Neuroscience 2023Quote: ... The revers transcription (RT) reaction was performed by M-MLV reverse transcription kit (Promega, Madison, WI, USA).
-
bioRxiv - Neuroscience 2023Quote: ... Total RNA was retrotranscribed into cDNA using M-MLV reverse transcriptase with Oligo(dT)15 primers (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... 1 µg of total RNA was used to synthesize cDNA using M-MLV reverse transcriptase kit (Promega) using the supplier’s protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... 250 ng to 1 μg of RNA were reverse transcribed using M-MLV Reverse transcrtiptase (Promega, #M1705) and oligo(dT ...
-
bioRxiv - Plant Biology 2023Quote: ... Protein marker was purchased from Fermentas (Beijing) and M-MLV reverse transcriptase was purchased from Promega (Beijing). Homologous recombinant kit was purchased from Clone Smarter (Beijing) ...
-
bioRxiv - Immunology 2023Quote: ... The total RNA extracted was reverse transcribed using M-MLV reverse transcriptase (Promega, Madi-son, WI, USA) and using random primers for initiation ...
-
bioRxiv - Genomics 2023Quote: ... 1 µg of Dnase-treated total RNA was reverse transcribed using the M-MLV Reverse Transcriptase (Promega) and Random Hexadeoxynucleotides (Promega ...
-
bioRxiv - Neuroscience 2024Quote: ... along with a Ten-m expression construct and Actin-GAL4 using the FuGENE HD transfection reagent (Promega). After 72 hours ...
-
bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesized using Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and universal Random hexamers (Promega). qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...