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Citations for Promega :
1 - 50 of 1615 citations for F actin uncapping protein LRRC16A CARMIL1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... protein samples were treated with PNGase F (Promega) according to manufacturer’s instruction under denaturing conditions ...
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bioRxiv - Immunology 2023Quote: ... Eluted proteins were then treated with PNGase F (Promega) according to manufacturer’s protocol and analyzed with SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were released from the intact proteins using 0.5 U/ µL N-glycosidase F (PNGase F, Elizabethkingia miricola, Promega), overnight ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 units of Peptide N-glycosidase F (PNGase F, Promega) enzymes according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and pGlosensor F-22 (Promega) were amplified and purified by Nucleospin plasmid TF grade (Takara bio ...
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bioRxiv - Biochemistry 2021Quote: ... chymotrypsin and peptide-N-glycosidase F (PNGase F) were obtained from Promega (Madison, WI, USA). Centrifugal filters (0.5 mL ...
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bioRxiv - Molecular Biology 2020Quote: ... HCT116 cells expressing the auxin-responsive F-box protein Oryza sativa TIR1 (OsTIR1) under the control of a Tet promoter were transfected using FuGENE HD (Promega) with a CRISPR/Cas9 plasmid targeting nearby the 1st ATG codon of the RIF1 gene (5’-TCTCCAACAGCGGCGCGAGGggg-3’ ...
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bioRxiv - Biochemistry 2023Quote: ... N- glycan release was achieved after digestion with PNGase F (1 U/10 µg protein at 37 °C overnight; Promega). Released N-glycans were hydrolysed with 25 µl of 100 mM ammonium acetate at pH 5 (1 h at RT) ...
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bioRxiv - Microbiology 2024Quote: Enzymatic deglycosylation of denatured protein samples was performed with PNGase F (1 Unit/μL) obtained from Promega (Madison, WI, USA). First ...
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bioRxiv - Molecular Biology 2020Quote: ... and PNGase-F (Promega Cat#V4831) prior to two-dimensional gel electrophoresis ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 U of PNGase F (Promega) in 5 µL 1x PBS was added and samples were incubated overnight at 37°C.
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bioRxiv - Immunology 2020Quote: ... IgG were deglycosylated using PNGase F (Promega) with 0.05 U of PNGase/μg of IgG ...
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bioRxiv - Biochemistry 2022Quote: Samples were treated with PNGase F (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Bioengineering 2024Quote: One hundred micrograms of each antibody were reduced with 50 mM DTT and treated with 2 µl PNGase F (Promega, USA) at 37°C for over 16h with stirring before being transferred to a new vial for LC-MS analysis ...
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bioRxiv - Biochemistry 2021Quote: ... 11S protein was detected with a rabbit polyclonal antibody from Promega, and Tom40 with a rabbit polyclonal antibody produced by Cambridge Research Biochemicals (Billingham ...
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bioRxiv - Immunology 2024Quote: Recombinant antibodies were purified using protein G magnetic beads (Promega #G7472), HiTrap protein G HP columns (Cytiva #17040401) ...
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bioRxiv - Immunology 2021Quote: ... and hFcγRIIIa-F variant (G9791) were purchased from Promega Corporation (Madison ...
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bioRxiv - Microbiology 2024Quote: ... with one half deglycosylated by PNGase F (Promega, #V4831) treatment for 1 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Actin-Renilla luciferease (rluc) and CMV-rluc (Promega) were used to control for transfection efficiency.
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bioRxiv - Cell Biology 2024Quote: ... proteins were detected with secondary peroxidase-conjugated antibodies (Promega, Madison, WI, USA) and ECL ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Following the manufacturer’s instructions for PNGase F (Promega Inc., V483A) treatment ...
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bioRxiv - Biochemistry 2024Quote: ... 1U of PNGase F obtained from Promega (Madison, WI, USA) was added to each sample to enzymatically release N-linked glycans from the glycoprotein substrates and incubated overnight at 37°C.
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bioRxiv - Plant Biology 2021Quote: ... Protein production was confirmed by western blot using an anti-HaloTag antibody (Promega). HaloTag-ligand conjugated magnetic beads (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were released by digestion with PNGase F (Promega, Madison, WI) overnight at 37 °C with vortexing ...
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bioRxiv - Cancer Biology 2022Quote: ... and N-glycans were released following the addition of PNGase F (Promega) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... the condensed supernatant was first incubated with PNGase F (Promega, Walldorf, Germany), heat-inactivated ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged or HA-tagged protein was detected using a commercially available mouse monoclonal antibody (Promega).
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bioRxiv - Plant Biology 2022Quote: ... Protein expression was confirmed by Western blot with Anti-Halo Tag monoclonal antibody (1:2000) (G9211, Promega).
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bioRxiv - Biochemistry 2020Quote: N-glycans were released from nMPO using Elizabethkingia miricola peptide-N-glycosidase F (Promega) (Jensen et al. ...
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were enzymatic released using 3.5 U Flavobacterium meningosepticum N-glycosidase F (Promega) in 20µl water/well ...
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bioRxiv - Immunology 2021Quote: ... F(ab′)2 fragments were generated after cleavage of IgG with IdeS protease (Promega) and then purified using TALON metal affinity resin (Takara ...
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bioRxiv - Molecular Biology 2024Quote: N-glycans were released from MonoQ fractionated FXa-cleaved CD52 using PNGase F (Promega). Briefly 10 U of PNGase F in MilliQ water was added to dried samples ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 40 µg of labeled peptides were then treated with PNGase F (Promega, #V4831) for 2 h at 37°C and taken forward to LC-MS/MS analysis.
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bioRxiv - Developmental Biology 2020Quote: ... hoT G actin ΔXbaI and HyGCaMP ΔNLS were ligated using T4 ligase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Kif9 and B-actin cDNAs were amplified by GoTag Green Master Mix (Promega) and with the following primers:
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
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bioRxiv - Immunology 2021Quote: ... The antibodies were purified from the culture supernatant 4 – 6 days later using protein G magnetic beads (Promega #G7472). Purified antibodies and antibody elution buffer (5 parts elution buffer (100 µM glycine-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Proteins separated on the second gel were transferred to a PVDF membrane and DivJ levels were measured using Western blots by probing the protein with anti-HaloTag antibody (Promega). Due to the requirement of growing cells in low phosphate media ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were transferred by semi-dry Western Blot onto PVDF membranes visualised with polyclonal antibody and BCIP/NBT (Promega, S3771).
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bioRxiv - Molecular Biology 2022Quote: ... Expression of Halo-bZIP and SBP-bZIP proteins were confirmed by western blotting using anti-HaloTag monoclonal antibody (Promega, G9211) and anti-SBPTag mouse anti-human antibody (MilliporeSigma ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of FgSte3-NLuc donor protein produced was also validated performing immunoblotting using anti-Nluc antibody (Promega cat#7000), see details below.
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bioRxiv - Immunology 2024Quote: ... 2 µg of anti-DNA-PKcs antibody or the corresponding IgG control was incubated with 70 ng of recombinant DNA-PKcs protein (Promega) in 500 µL of lysis buffer (20 mM Tris–HCl [pH 7.4] ...
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bioRxiv - Microbiology 2022Quote: ... Relative F-Luc activity compared to R-Luc was quantified using a dual luciferase assay (Promega, E1960).
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Cell Biology 2023Quote: ... Dystrophin gene editing was verified by PCR for mouse dystrophin using primers Dmd_i22-i23.F/ Dmd_i22-i23.Rand GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed with primers Dmd_e22-e24.F / Dmd_e22-e24.R using GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then digested with LysC at 1ug/100ug protein (Promega) for 3 h at room temperature (RT ...