Labshake search
Citations for Promega :
1 - 50 of 679 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
-
bioRxiv - Biochemistry 2021Quote: ... LgBiT subunit (Promega) was fused at the C-terminus of PTH2R connected by a 20-amino acid linker ...
-
bioRxiv - Molecular Biology 2022Quote: ... LgBiT subunit (Promega) was fused at the C terminus of RXFP4 connected by a 15-amino acid polypeptide linker ...
-
bioRxiv - Biochemistry 2022Quote: ... Rat Gβ1 with an N-terminal MHHHHHHSSGLVPRGSHMASHHHHHHHHHH-tag (His16) was fused with the SmBiT subunit (peptide 86, Promega)20 via a 15 amino acid (GSSGGGGSGGGGSSG ...
-
bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...
-
bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
-
bioRxiv - Molecular Biology 2024Quote: MBP-Q32-HttEx1 and MBP-Q44-HttEx1 were cleaved to release soluble HttEx1 by using Factor Xa (FXa; Promega, Madison, WI) at 400:1 (Fused protein ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.5μl of GTP (100mM, Promega®), 1μl of Ribolock™ (40U/μl ...
-
bioRxiv - Neuroscience 2021Quote: Orthophosphate release assays (Promega V2471) used Malachite green and molybdate with spectrophotometric detection of liberated free orthophosphate from test phosphopeptides compared to control/comparison phosphopeptides with assessments for the times indicated ...
-
bioRxiv - Evolutionary Biology 2020Quote: To generate the pGl3-STK38-P luciferase reporter,1.2 kb of DNA upstream the STK38 TTS promoter region with ZNF611 binding motif was amplified by polymerase chain reaction (PCR) and cloned into the Firefly luciferase reporter plasmid pGL3-Basic (Promega) using KPN1 and XHO1 restriction sites.0.7 kb of DNA upstream the STK38 TTS promoter region without ZNF611 binding motif was cloned into pGL3-Basic using same restriction sites to generate the pGl3-STK38-ΔP luciferase reporter ...
-
bioRxiv - Physiology 2022Quote: ... 1.15 μg of purified AQP2 or AQP2 S256A COOH-tail peptide were mixed with either RSK (16 nmol) or PKA catalytic subunit (5.6 nmol) (Promega, Madison, WI) in a total volume of 12 μl reaction buffer containing 40 mM Tris pH 7.5 ...
-
bioRxiv - Cell Biology 2020Quote: Colorimetric LDH release assay kit (Promega G1780) was performed according to manufacturer’s instructions.
-
bioRxiv - Cell Biology 2021Quote: Colorimetric LDH release assay kit (Promega G1780) was performed according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... in which dissociation of a Gα subunit from a Gβγ subunit was monitored by a NanoBiT system (Promega). Specifically ...
-
bioRxiv - Molecular Biology 2022Quote: ... 50 ng/well of chaperone RTP1S,105 50 ng/well of the G protein subunit Gαolf,106–107 olfactory G protein subunit Gγ13,108 and 50 ng/well of pGloSensorTM-22F (Promega, Madison, USA).109 The utilized pGloSensor™-22F is a genetically engineered luciferase with a cAMP-binding pocket ...
-
bioRxiv - Biophysics 2024Quote: ... in which the interaction between a Gα subunit and a Gβγ subunit was monitored by the NanoBiT system (Promega). Specifically ...
-
bioRxiv - Immunology 2023Quote: The release of lactate dehydrogenase (LDH, Promega, USA) into the culture medium was quantitated using Cyto Tox 96® Non-Radioactive Cytotoxicity Assay (Promega ...
-
bioRxiv - Biochemistry 2022Quote: ... in which the interaction between the Gα subunit and the Gγ subunit was monitored by the NanoBiT-based enzyme complementation system (Promega). Specifically ...
-
bioRxiv - Immunology 2022Quote: ... and pyroptosis was determined by lactate dehydrogenase (LDH) release (Promega) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... CytoTox 96 LDH-release assay kit (G1780) was from Promega. Efficient eukaryotic transfection reagent VigoFect was purchased from Vigorous Biotechnology (Beijing) ...
-
bioRxiv - Immunology 2024Quote: ... LDH release was assessed using the Cytotoxicity Detection Kit (Promega) following the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2023Quote: GTP turnover was analyzed using GTPase-Glo Assay kit (Promega Cat# V7681). Briefly ...
-
bioRxiv - Microbiology 2023Quote: The GTP levels were measured using a GTPase-Glo Assay kit (Promega) which employs an ATP-linked luciferase reaction ...
-
bioRxiv - Molecular Biology 2024Quote: The GTP turnover assay was adapted from the GTPase-GloTM assay (Promega) as described previously(33) ...
-
bioRxiv - Microbiology 2021Quote: ... Eukaryotic in vitro Nluc expression was performed with TnT-T7 Quick Coupled Transcription/Translation System (# L1170, Promega) with 100 ng of pT7CFE1-NLuc plasmid DNA ...
-
bioRxiv - Biochemistry 2022Quote: ... the above PTH1R construct was added the LgBiT subunit (Promega) at the C terminus of PTH1R ...
-
bioRxiv - Biochemistry 2023Quote: ... the above PTH1R construct was added the LgBiT subunit (Promega) at the C terminus of PTH1R with a 17-amino acid linker ...
-
bioRxiv - Immunology 2021Quote: ... CytoTox 96 LDH-release assay kit (G1780) was purchased from Promega.
-
bioRxiv - Molecular Biology 2022Quote: Extracellular glutamate release was determined according to manufacturer’s protocols (Promega, J8021). Alternatively ...
-
bioRxiv - Neuroscience 2021Quote: ... iN cytotoxicity was measured using lactate dehydrogenase (LDH) release assay (Promega) following 48h treatment with vehicle (DMSO) ...
-
bioRxiv - Microbiology 2023Quote: ... an LDH release assay was performed following the manufacturer’s protocol (Promega). Briefly ...
-
bioRxiv - Microbiology 2020Quote: ... incubating and chromogenic reaction generating were similar to the ACE2 assay instead of that primary antibody binding to antigen was not required and that HRP-conjugated goat anti-human IgG antibody (Promega) would be used as the secondary antibody to detect binding of CB6 antibody to the antigens.
-
bioRxiv - Cell Biology 2020Quote: ... LDH release analysis was performed using CytoTox96 Non-radioactive Cytotoxicity Assay (Promega). Protein concentrations were assayed using BCA Protein Assay Kit (Quantum Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... Glutathione release was measured using the GSH-Glo glutathione assay (Promega; V6912), as per the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... Endogenous UDP release was detected by UDP-GloTM Glycosyltransferase Assay kit (Promega).
-
bioRxiv - Cancer Biology 2023Quote: ... Endogenous UDP release was measured by UDP-GloTM Glycosyltransferase Assay kit (Promega). HDAC activity was detected by HDAC Cell-Based Activity Assay kit (#600150 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Extracellular ATP release using the RealTime-Glo Extracellular ATP Assay (Promega #GA5010) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... Viability was assessed via ATP-release assay (CellTiter-Glo 3D, Promega #PRG9683) after PBS washes (Gibco #14190144 ...
-
bioRxiv - Biochemistry 2021Quote: Assays for GAP-stimulated GTP hydrolysis were performed using the GTPase-Glo kit (Promega) based on the protocol by Mondal et al(48) ...
-
bioRxiv - Biophysics 2021Quote: ... the NanoBiT tethering strategy was applied by fusing a LgBiT subunit (Promega) at the receptor C-terminus using homologous recombination (CloneExpress One Step Cloning Kit ...
-
bioRxiv - Cell Biology 2020Quote: ... cell death was assayed by release of LDH into cell culture media (Promega CytoTox 96® Non-Radioactive Cytotoxicity Assay) ...
-
bioRxiv - Microbiology 2021Quote: ... Cytotoxicity was quantified colorimetrically with the CytoTox96 lactate dehydrogenase (LDH)-release kit (Promega). The percentage of cytotoxicity was calculated with the formula ...
-
bioRxiv - Cancer Biology 2020Quote: ATP release was measured using the Kinase-Glo® Luminescent Kinase Assay (Promega) as per the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: LDH release was determined by the colorimetric CytoTox 96 Cytotoxicity Assay kit (Promega). After BrainSpheres infection ...
-
bioRxiv - Immunology 2020Quote: LDH release was measured using the CytoTox One homogenous membrane integrity assay (Promega). Cytotoxicity was calculated as a function of LDH release using the following formula ...
-
bioRxiv - Genetics 2024Quote: ... LDH release was quantified using CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega #G1780) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... To identify both light chains we performed subcloning of the light chain PCR products into pGEM-T-Vector System (Promega, A3600). First ...
-
bioRxiv - Microbiology 2020Quote: Apical supernatants were assessed for LDH release using Promega Cytox Kit (Promega, Madison, WI) according to manufacturer’s instructions (33).
-
bioRxiv - Immunology 2023Quote: Lactate dehydrogenase (LDH) release was measured using LDH-Glo Cytotoxicity Assay kit (Promega, J2380) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: LDH release into culture supernatant was measured using the LDH-Glo Cytotoxicity Assay (Promega) according to the manufacturer’s instructions ...