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Citations for Promega :
201 - 250 of 2718 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... 20 ng/μL LysC and 10 ng/μL Trypsin (Promega) were added to each sample and incubated for 16 hours at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... After extraction a Turbo DNase (Promega) digest was performed as per the manufacturer’s instruction and total RNA yield was quantified using a Qubit RNA broad spectrum assay kit as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... RNA of roots and nodule primordia was extracted using a TRIzol (Invitrogen)-adapted ReliaPrep RNA extraction kit (Promega), as previously described (53) ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted using a TRIzol (Invitrogen)-adapted ReliaPrep RNA extraction kit (Promega) [39] ...
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bioRxiv - Plant Biology 2023Quote: ... LUC activity was measured with 20 mL of protein sample and 100 mL of LUC assay reagent (Promega). Promoter activation was calculated as (rate of MUG accumulation / LUC luminescence ...
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bioRxiv - Immunology 2021Quote: ... after added 100 mL of Caspase-Glo 3/7 reagent (Promega, USA), the plate was gently shaken at room temperature for 2 h and the luminescence was measured by a plate-reading luminometer.
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bioRxiv - Biochemistry 2022Quote: ... and one was treated with 100 µg/mL of RNase A (Promega) at 37 °C for 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.12 mM EDTA and 100 µg/mL RNAse A (Promega A797c, Promega) and incubated 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.12 mM EDTA and 100 µg/mL RNAse A (Promega A797c, Promega) and incubated 30 minutes at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were resuspended in 4 ml of Blob100 buffer plus 80 units/ml RNasin Plus (Promega, Flitchburg, WI, USA) and lysed under liquid nitrogen in a 6870 Freezer Mill (Spex ...
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bioRxiv - Microbiology 2019Quote: ... Digestion product was cooled and transferred to a pre-chilled 15 ml tube containing 4 ml 1.1X Ligase Buffer (Promega) and 1% Triton X-100 ...
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bioRxiv - Biochemistry 2019Quote: JAK2-deficient ϒ2A human fibrosarcoma cells were transfected with different human JAK2-hemagglutinin (HA) constructs in pCIneo vector (100 ng per 12-well plate well) with FuGENE HD (Promega). After 48 hours ...
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bioRxiv - Microbiology 2019Quote: ... of 100 ng of RNA was performed as previously described [49] using Moloney Murine Leukemia virus (M-MLV) reverse transcriptase (Promega), with an extension temperature of 42°C for 60 min and random hexamer (IDT) ...
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bioRxiv - Genomics 2020Quote: ... Zymo Research EZ DNA Methylation kit was used for bisulfite conversion on 100 ng of DNA along with 0.5% w/w of unmethylated lambda DNA (Promega, US), included to evaluate bisulfite conversion efficiency ...
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bioRxiv - Biochemistry 2020Quote: ... Gel pieces were cooled on ice and rehydrated with 100 μ L of 10 ng/μL Trypsin Gold (Promega, UK) for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells were seeded in 24-well tissue culture plates at a density of 1.6 x 105 cells/well and transfected with 50-100 ng/well of pNL4-3-GFP using Fugene HD (Promega) transfection reagent ...
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bioRxiv - Cell Biology 2020Quote: ... and the proteins were on-bead digested using 5 µl of Sequencing Grade Trypsin (100 ng/µl in 10 mM HCl, Promega). The digestion was carried out at 37°C over night ...
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bioRxiv - Plant Biology 2022Quote: ... 100 ng total DNA was added to a 20 μl PCR containing 10 μl GoTaq® Master Mixes (Promega, USA) and 0.1 μM of each primer pair ...
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bioRxiv - Microbiology 2020Quote: ... Reduced and alkylated proteins from band slices excised from the entire separation profile were in-gel digested at 37°C overnight with 100 ng trypsin (modified sequencing grade, Promega). Supernatants were dried in a vacuum centrifuge and resuspended in 0.1% TFA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then transiently-transfected with 100 ng/well cDNA encoding NLuc/NRP1 or NLuc/VEGFR2 using FuGENE (Promega, USA) according to the manufacturer’s instructions and incubated for a further 24h at 37°C/5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 ng of DNase treated RNA library was added to 100 nM of Halo-tagged proteins immobilized onto magnetic resin (Promega). The volume of each binding reaction was 100µl in SEQRS buffer containing 200 ng yeast tRNA competitor and 0.1 units of RNase inhibitor (Promega) ...
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bioRxiv - Microbiology 2019Quote: ... Pelleted cells suspended gently in 100 μl of TEAB were mixed with 50 ng/µL of sequencing grade modified porcine trypsin (Promega), and digestions were incubated at 37 °C for 15 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Linear replicon DNA (500 ng) was added to transcription reactions at a final volume of 100 μl containing the following: Transcription Optimised Buffer (Promega), 10 mM DTT (Promega) ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were on-bead digested using 5 μl of Sequencing Grade Trypsin (100 ng/μl in 10 mM HCl, Promega). The digestion was carried out in a microwave instrument (Discover System ...
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bioRxiv - Cell Biology 2022Quote: ... The slurry was then diluted four times with 20mM Tris-HCl with 2mM CaCl2 before digestion overnight with 100 ng sequencing grade trypsin (Promega). The samples were then centrifuged for peptide extraction from the supernatant which were then subject to liquid chromatography LC-MS (PROXEON coupled to a QExactive mass spectrometer ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins bound to magnetic beads were washed twice with 100 μL of 25 mM NH4HCO3 and on-beads digested with 200 ng of trypsine/LysC (Promega) for 1 hour in 100 μL of 25 mM NH4HCO3 at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The gel pieces were dehydrated using 100% ACN before 25 µl of 12 ng/µl sequence grade modified trypsin porcine (Promega) in 25 mM ABC was added ...
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bioRxiv - Molecular Biology 2023Quote: ... The library preparation involved using 100 ng of genomic DNA spiked with 1% (w/w) of unmethylated lambda DNA from Promega. Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... For transient expression experiments each well was transfected with 100 ng of DNA using 1:3 ratio of FUGENE HD (#E2311, Promega). Twenty-four hours after transfection or doxycycline stimulation ...
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bioRxiv - Microbiology 2023Quote: For NanoBIT experiments 12,000 HeLa cells were seeded in 96-well black plates and each well was transfected with 100 ng of total DNA using 1:3 ratio of FUGENE HD (#E2311, Promega). Different amounts of plasmid DNA were used to achieve comparable expression of LgBiT/FLAG-tagged and SmBiT/V5-tagged proteins ...
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bioRxiv - Cell Biology 2022Quote: ... on-bead digestion was performed by addition of 100 μL trypsinization buffer (50 mM ammonium bicarbonate, Sigma and 300 ng trypsin per column, Trypsin-gold V5280 Promega) for 1 h at 23°C ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega, cat# A6110). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2024Quote: ... Tail DNA from offspring was genotyped for the presence of the BAC transgene by analyzing 100 ng of tail DNA with the GoTaq PCR reaction kit (Promega) according to the manufacturer’s instruction by adding forward primer 5′-GAGGCTCGGGTGCGGCAGCTC −3′ and reverse primer 5′-GTGCAGTGTGAGCACCTGCTGTC-3′ for PCR with an initial denaturation for 3 min at 95 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Integrated sgRNAs in each lymphoma sample were amplified from 100 ng of genomic DNA using GoTaq Green Master Mix (Promega) and indexing primers with unique overhangs (12) ...
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bioRxiv - Cell Biology 2023Quote: ... dilute with 4 volumes of 20 mM Tris-HCl pH8 / 2 mM CaCl2 before overnight digestion at room temperature with 100 ng sequencing grade trypsin (Promega). Samples were centrifuged ...
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bioRxiv - Cell Biology 2024Quote: ... The protein pellets were resuspended in 50 μl 100 mM TEAB and digested with 200 ng trypsin (Promega sequencing grade) overnight at 37°C.
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bioRxiv - Microbiology 2021Quote: ... Five ml of BHI broth containing 10 μg/ml tetracycline was inoculated with a single colony and genomic DNA was extracted (Wizard DNA extraction kit, Promega). Genomic DNA was sequenced by paired-end joining Illumina (Biomics Platform of the Institut Pasteur ...
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bioRxiv - Developmental Biology 2021Quote: ... and 175 μg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Promega). Reactions were stopped with three PBS rinses ...
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bioRxiv - Developmental Biology 2022Quote: ... and 175 μg/ml 5-bromo-4-chloro-3- indolyl-phosphate (BCIP) (Promega). Reactions were stopped as the signal became apparent with three PBS rinses ...
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bioRxiv - Molecular Biology 2019Quote: ... sliced and dehydrated twice using 50% ACN followed by 100% ACN and the proteins digested with ~100 µl of Trypsin solution (10 µg/ml, Promega) in 25 mM ammonium bicarbonate by incubating overnight at 37° C ...
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bioRxiv - Genetics 2022Quote: ... was transiently transfected with the pRL-TK Renilla internal control vector (50 ng) using 4 μL Viafect™ transfection reagent (Promega) into suspension cell lines or adherent cell lines ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was digested by incubation overnight at 37°C with trypsin (5 ng/ ml, Promega, Madison, WI) in 50 mM NH4HCO3 (pH 7.8) ...
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bioRxiv - Neuroscience 2020Quote: ... in DEPC-treated phosphate buffer (PB) with 0.5% Triton X-100 supplemented with RNasin (40Ul/µl stock, 5 ml/ml of buffer, Promega). We rinsed sections ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Recombinant baculovirus was produced by transfecting recombinant bacmids (2-3 μg) into Sf9 cells (5 mL, density of 4×105 cells per mL) using FuGENE HD Transfection Reagent (Promega) and Opti-MEM Reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: Forty-nine confirmed Bp isolates were subjected to DNA extraction using the Wizard Genomic Extraction Kit (Promega, USA). For whole genome sequencing (WGS) ...
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bioRxiv - Plant Biology 2023Quote: ... multiflorum samples using a commercial DNA extraction kit (Wizard HMW DNA Extraction Kit, Promega Corporation, Madison, WI, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were diluted 4-fold with 100 mM ammonium acetate and digested with sequence-grade trypsin (Promega) overnight at RT at an enzyme:substrate ratio of 50:1 (w/w) ...
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bioRxiv - Plant Biology 2020Quote: ... The columns were capped at the bottom and 200 µl AmBic containing 4 ng/µl of Trypsin + LysC (Promega Catalog number V5073) was added to each sample and incubated in a 37°C shaker for 16 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... and Maxwell nucleic-acid extraction instrument (Promega). Nanodrop quantified RNA was checked by Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies) ...
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bioRxiv - Plant Biology 2020Quote: ... the SV Total RNA Extraction Kit (Promega) was used following the product specifications ...