Labshake search
Citations for Promega :
1 - 50 of 101 citations for Diphtheria Toxin CRM197 Mutant since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... point mutant (Promega). Quantitative real-time PCR was performed using GoTaq qPCR Master Mix (Promega ...
-
bioRxiv - Molecular Biology 2019Quote: ... Point Mutant (Promega, M3681) and random hexamers (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... Point Mutant (Promega, M3681) and random hexamers (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... Point Mutant (Cat. # M3683, Promega). DNAse treated RNA ...
-
bioRxiv - Biochemistry 2024Quote: ... Toxin sensitivity was measured with CellTiter-Glo reagent (Promega) according to manufacturer protocol ...
-
bioRxiv - Plant Biology 2019Quote: ... (RNase H minus, Point Mutant, Promega) after annealing with an anchored oligo(dT)18 primer as described by Wirth et al ...
-
bioRxiv - Molecular Biology 2022Quote: The proliferation of Ba/F3 expressing WT or mutants TpoR mutants was assessed by Cell-Titer-Glo luminescent cell viability assay (Promega). Prior to the experiment ...
-
bioRxiv - Immunology 2020Quote: ... and M-MLV RT RNAse (H-) Point Mutant (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... and M-MLV Reverse Transcriptase (RNase H Minus, Point Mutant; Promega). Quantitative (q)RT-PCR reactions for target genes were prepared using specific primer (Oligomer ...
-
bioRxiv - Microbiology 2020Quote: ... or Vpr F34I/P35N mutant using 6 μl Fugene-6 (Promega). For KPNA-cargo IPs HEK293T cells were grown in 10 cm dishes and co-transfected with 1 μg of a plasmid expressing FLAG-tagged KPNA1 ...
-
bioRxiv - Genetics 2022Quote: ... and two isoform1 mutant plasmids using FuGENE® HD (Promega, E2311) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Moloney Murine Leukemia Virus Reverse Transcriptase RNase H-Point Mutant (Promega) was used for cDNA synthesis ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmids encoding different Ocrl1 mutants were transfected using Fugene 6 reagent (Promega) according to manufacturer’s instructions.
-
bioRxiv - Neuroscience 2020Quote: ... Wildtype and mutant constructs were then cloned into luciferase vector pGL4.25 (Promega). Luciferase plasmids were transfected into N2A cells ...
-
bioRxiv - Cell Biology 2019Quote: ... The normal and mutant TMEM135 promoters were cloned into the pGL4.17 vector (Promega) using SacI and XhoI restriction enzyme sites ...
-
bioRxiv - Biochemistry 2020Quote: ... 1 μg pHAGE-HA-FIP200-IRES-puro and mutants with FuGENE HD (Promega) for 18 hrs and then treated with 10 μM Oligomycin (Calbiochem) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with NOCT N-terminal mutant constructs using Fugene HD (Promega) in a ratio of 3 µL transfection reagent per 1 µg DNA ...
-
bioRxiv - Cell Biology 2023Quote: ... wild-type and mutant tepsin-myc constructs were transfected using Fugene 4K (Promega) at 1.5:1 Fugene:DNA ratio following manufacturer protocol ...
-
bioRxiv - Microbiology 2019Quote: DNA was extracted from mutant isolates using the Wizard Genomic DNA Purification Kit (Promega). Briefly ...
-
bioRxiv - Cell Biology 2021Quote: ... cDNA libraries were generated using M-MLV RT [H-] Point mutant (Promega, WI, USA) according to the manufacturer’s directions ...
-
bioRxiv - Developmental Biology 2019Quote: ... we produced recombinant H3 mutant proteins from mRNAs using rabbit reticulocyte lysate (Promega L4600). After 3h of incubation at 4°C in interphase extracts followed by another 3h incubation with anti-HA beads ...
-
bioRxiv - Genetics 2020Quote: Full-length wild-type and mutant Csde1 5’UTRs were inserted pGL3 (Promega, E1751) plasmid in between EcoRI and NcoI sites upstream of Firefly luciferase gene ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: HEK-293 cells transiently transfected with PAR2-YFP or mutant PAR2-YFP (Fugene 6, Promega) were sub-cultured onto 35-mm glass-bottom culture dishes (MatTek Corporation ...
-
bioRxiv - Microbiology 2023Quote: ... control and CypA mutant HT1080 cells were lysed by adding a passive lysis buffer (Promega) supplemented with protease inhibitor cocktail (Roche) ...
-
bioRxiv - Biophysics 2021Quote: ... cells were transfected with pcDNA3-Kv7.2 WT or mutant (0.5 μg) using FuGENE6 transfection reagent (Promega). At 24 hrs post-transfection ...
-
bioRxiv - Biophysics 2021Quote: ... cells were transfected with pcDNA3-Kv7.2 WT or mutant (0.8 μg) using FuGENE6 transfection reagent (Promega). At 24 hr post-transfection ...
-
bioRxiv - Biophysics 2020Quote: CosM6 cells were transfected with ∼1 µg of wild type or mutant constructs using FuGENE6 (Promega) and were patched within 1-2 days after transfection ...
-
bioRxiv - Synthetic Biology 2019Quote: The wild type and mutant promoter of miR-146a-5p were constructed into pGL3 vector (Promega). The pGL3-WT or pGL3-mut were co-transfected with pRL-TK using lipofectamine® 3000 ...
-
bioRxiv - Biochemistry 2019Quote: Wild-type and mutant AP2ΔµC complexes were expressed in BL21 (DE3) pLysS strain (Promega, Madison, WI) by induction at 23 °C overnight ...
-
bioRxiv - Cancer Biology 2021Quote: ... wild type or mutant 3′-UTR of DKK3 was cloned into the psicheck-2 vector (Promega). HCT116 cells were transfected with and wild-type or mutant 3′-UTR-luc by using Lipofectamine 3000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... WT or mutant promoter of human Rpl28 gene was cloned into pGL-3-basic (Promega, E1751). The number is relative to transcriptional start site ...
-
bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from AR16 and mutant strains using Wizard Genomic DNA Purification Kit (Promega) and transformed into B ...
-
bioRxiv - Microbiology 2024Quote: ... the genomic DNA of the mutants was isolated using a Wizard Genomic DNA Purification Kit (Promega). The isolated genomic DNA was analyzed by agarose electrophoresis and quantified using a NanoDrop spectrophotometer ...
-
bioRxiv - Molecular Biology 2023Quote: ... The wild-type and mutants of MCHR1 and MCHR2 were constructed into the pcDNA6.0 vector (Promega) for functional assays.
-
bioRxiv - Bioengineering 2020Quote: ... Homozygous lpat2-3 T-DNA insertional mutants were identified by PCR using Promega PCR Master Mix (Promega) and combinations of the gene specific and T-DNA left border primers pairs ...
-
bioRxiv - Biophysics 2021Quote: ... 50 nM of WT condensin or EQ mutant was incubated with 50 ng/μL λ-DNA (Promega) for 15 min in 40 mM TRIS-HCl pH 7.5 ...
-
bioRxiv - Microbiology 2020Quote: Genomic deoxyribonucleic acid (gDNA) was isolated from each mutant using the Wizard® gDNA purification kit (Promega). Illumina NextSeq was then performed by the Genomic Services Facility at Indiana University Center for Genomics and Bioinformatics ...
-
bioRxiv - Microbiology 2023Quote: DNA of selected phage-resistant mutants was isolated using a Wizard Genomic DNA purification kit (Promega Corp.) according to the guidelines provided by the manufacturer ...
-
bioRxiv - Cell Biology 2023Quote: ... or mutant β2AR INTAL and a plasmid encoding a cyclic-permuted luciferase reporter construct (pGloSensor-20F, Promega) and luminescence values were measured ...
-
bioRxiv - Microbiology 2019Quote: ... 48 hours later cells were transfected with a plasmid encoding HA of the FPV mutant using Fugene (Promega). 24 hours later cells were labelled for 2 hr with 200 µCi/ml [3H]-palmitic acid (9,10-3H(N) ...
-
bioRxiv - Cell Biology 2021Quote: ... Donor 293T cells were co-transfected with 1.5 µg of plasmids encoding different untagged S mutants and 0.6 µg of pmCherry-N1 using 6 µL of Fugene 6 following the manufacturer’s instructions (Promega). Acceptor Vero cells expressing human ACE246 ...
-
bioRxiv - Biochemistry 2019Quote: ... 0.2 μg pLEXm plasmid encoding WT or mutant KAP1 and 0.4 ng plasmid encoding Renilla luciferase using 1.5 μl of FuGENE 6 (Promega). Luciferase activity was measured 48 h post-transfection in cell lysates with the Dual Luciferase assay kit (Promega ...
-
bioRxiv - Biophysics 2019Quote: CosM6 cells were transfected with 1-2 µg of pcDNA3.1-Kir2.2-K62W or pcDNA3.1-Kir2.2-K62W-G178D mutants with an addition of 0.4 µg of pcDNA3.1-GFP per 35mm Petri dish using FuGENE6 (Promega). The cells were used for patching within 1-2 days after transfection ...
-
bioRxiv - Molecular Biology 2022Quote: ... point mutant which is used in cDNA synthesis with long RNA templates of more than 5kb (Promega #M3681) and primer SMART-dT18VN ...
-
bioRxiv - Cancer Biology 2023Quote: ... 3’UTR Wild and 3’UTR-Mutant were incorporated into XbaI restriction site of PGL3-control vector (Promega) expressing firefly luciferase ...
-
bioRxiv - Cancer Biology 2021Quote: ... wild-type and mutant MMP2 3′-UTRs were synthesized and recombined into pmirGLO Luciferase vectors (Promega, Madison, WI, USA). Then pmirGLO-Wt/pmirGLO-Mt or miR-1299 mimics/miR-NCs were co-transfected into EC9706/KYSE30 cells ...
-
bioRxiv - Cell Biology 2021Quote: HeLa cells were transfected with WT or mutant C10 constructs using the FuGENE 6 transfection reagent (Promega, cat# PRE2691). The cells were incubated at 37 C at least overnight before downstream analysis.
-
bioRxiv - Plant Biology 2021Quote: The RNA was transcribed into cDNA using the M-MLV Reverse Transcriptase (RNase H- Point Mutant, Promega, Walldorf, Germany). qPCR was performed using a Realplex Mastercycler system (Eppendorf ...
-
bioRxiv - Cancer Biology 2019Quote: ... Indicated amounts of HMGA2 and mutant HMGA2 protein were incubated with 100ng of negatively supercoiled Renilla reporter plasmid (Promega) for 30mins at 37°C in a buffer containing 10mM Tris-HCl ...
-
bioRxiv - Developmental Biology 2022Quote: ... All enhancer or mutant enhancer reporter constructs (200ng) were transfected with 20ng of pRL (Renilla luciferase control vector, Promega) and either empty vector (pcDNA3.1 ...