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Citations for Promega :
1 - 50 of 1013 citations for Dig 6C SAH 1a b c since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... SAH can be quantified by the MTase-Glo assay (Promega). Quantification is based on the conversion of SAH to adenosine diphosphate (ADP) ...
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bioRxiv - Biochemistry 2023Quote: ... The SAH production was determined with MTase-Glo methyltransferase kit (Promega) using 8 uL of the stopped reaction ...
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bioRxiv - Biochemistry 2023Quote: ... The SAH production was measured using MTase-Glo methyltransferase kit (Promega) using 8 µL of the stopped reaction ...
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bioRxiv - Biochemistry 2021Quote: ... MTase-Glo™ Methyltransferase Assay Kit containing SAM and SAH were purchased from Promega. All other chemicals were purchased from readily available commercial sources and were used without further purification ...
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bioRxiv - Cancer Biology 2022Quote: ... Enzyme activity was assessed by SAH production as determined by luminescence through the MTase-Glo assay (Promega). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... Reaction progress was measured as a function of SAM to SAH conversion using the MTase Glo Kit (Promega). KM values for SAM were determined by the same method but holding substrate concentrations constant at 100 μM and varying the concentration of SAM ...
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bioRxiv - Genetics 2023Quote: ... the samples could be reamplified and reanalyzed by either Powerplex® Fusion 6C system (Promega) or VerifilerTM amplification kit (Applied Biosystem ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were stopped by addition of TFA to 0.1% and the production of SAH was measured using the MTase-Glo Methyltransferase Assay kit (Promega) according to the manufacturer’s instructions using a Hidex Sense microplate reader..
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bioRxiv - Developmental Biology 2021Quote: ... DIG-labeled RNA probes were produced using DIG-UTP by in vitro transcription with T3 RNA polymerase (Promega, Catalog No. P208C) according to the Roche Applied Science’s protocol (Roche Applied Science ...
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bioRxiv - Cancer Biology 2021Quote: ... we used CellTiter-Glo® (only in Fig. 1A) and CellTiter-Glo® 2.0 assays (Promega). To detect the cell death activation ...
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bioRxiv - Developmental Biology 2021Quote: ... DIG-labeled RNA probes were synthesized with the Riboprobe Systems (Promega) and DIG RNA Labeling Mix (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... b-gal (Promega, Z3781), p21 (BD Pharmingen ...
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bioRxiv - Developmental Biology 2023Quote: ... and antisense DIG-labeled RNA was synthesized using SP6 RNA polymerase (Promega).
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bioRxiv - Microbiology 2020Quote: ... Two allelic replacement vectors—pFtsH1G489G carrying two synonymous “shield” mutations and pFtsH1G489C carrying a further G to T mutation at base 1465 (Figure 1A)—were created by cloning a PCR amplified segment of Pfftsh1 into pGEM-T Easy (Promega). Quickchange XL (Clontech ...
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bioRxiv - Microbiology 2022Quote: In vitro translation assays of the wild type or mutant CrPV-1A RNAs were performed in Spodoptera frugiperda 21 (sf-21) insect cell extract (Promega). Briefly ...
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bioRxiv - Plant Biology 2022Quote: ... A digoxigenin (DIG)-labeled antisense (AS) probe was synthesized using the T7 polymerase (PROMEGA), for hybridization on pre-parasitic stage 2 juvenile nematodes (ppJ2) ...
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bioRxiv - Genomics 2024Quote: ... Antisense DIG-tagged probes were synthesized by in vitro transcription using T3 polymerase (Promega) and purified using a Sephadex G-50 column ...
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bioRxiv - Microbiology 2024Quote: ... preceding an immunoglobulin gene heavy chain variable region that is commonly found in commercial vectors such as in the pRL-CMV plasmid from Promega (Fig 1A) [53–55].
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bioRxiv - Genetics 2021Quote: ... DIG-labelled RNA probes were produced by in vitro transcription with T3-RNA polymerase (Promega). The Crabp1 probe template was amplified from a plasmid containing Crabp1 cDNA sequence ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti b-galactosidase (1:500; Promega # Z3781), chicken anti b-galactosidase (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... anti-b-111-tubulin (mouse, Promega G712A, 1:500), Cleaved caspase-3 (rabbit ...
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bioRxiv - Neuroscience 2020Quote: ... digoxigenin (DIG)-labeled riboprobes were generated with a commercial transcription kit (Riboprobe System, P1440, Promega, San Luis Obispo, CA) and DIG RNA labeling mix (Roche REF# 11277073910 ...
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bioRxiv - Developmental Biology 2024Quote: ... Next the DIG-labeled RNA probes were synthesized by the transcription reaction using Riboprobe Systerm-T7 kit (Promega, P1440) and DIG labeled UTP (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Sample B was treated with 1 µg/ml trypsin (Promega). Sample C was pre-treated with 1% Triton X-100 (EMD-Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... Sample B was treated with 1μg mL-1 trypsin (Promega). Sample C was treated with 1% Triton X-100 (TX100 ...
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bioRxiv - Microbiology 2024Quote: ... NSP2 B to J were cloned in pCI-Neo (Promega) between EcoRI/NotI restriction sites ...
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bioRxiv - Microbiology 2024Quote: ... NSP5 B to J were cloned in pCI-Neo (Promega) between MluI/NotI restriction sites ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were isolated by resuspending cell pellets in 200 μL Dig-wash buffer (CUT&Tag wash buffer supplemented with 0.05% digitonin (Promega, G9441) and 0.05% IGEPAL CA-630 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... and the purified templates were used to produce single stranded digoxigenin (DIG)-labelled RNA probes with the T7 (P2075) and Sp6 (P1085) transcription polymerases from Promega. Transcription was performed after manufacturer’s instructions except for the use of the DIG-labeling mixture (11277073910 ...
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bioRxiv - Genetics 2020Quote: ... The DNA templates used for DIG-labeled STX18-AS1 RNA probe in vitro syntheses (Supplementary file) with T3 RNA polymerase (Promega) were amplified from the coding sequence of STX18-AS1 from human foetal hearts cDNA (obtained from HDBR) ...
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bioRxiv - Developmental Biology 2021Quote: ... using primers 1 and 2 (Supplementary Table) and used for making a Digoxigenin (DIG)-labeled FISH probe using T3 RNA polymerase (P2083, Promega). After T3 RNA polymerase reaction at 37°C for 4 hours ...
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bioRxiv - Cell Biology 2022Quote: Digoxin-labeled RNA probes were in vitro transcribed as per manufacturers’ instructions (DIG RNA labeling Mix, Roche; T7 polymerase, Promega; SP6 Polymerase, Promega) and purified by G-50 column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... Digoxin-labeled RNA probes were in vitro transcribed as per manufacturers’ instructions (DIG RNA labeling Mix, Roche; T7 polymerase, Promega; SP6 Polymerase, Promega) and purified by G-50 column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2022Quote: ... rLys-C (Promega) was added at 1:50 enzyme wt:sample wt to the lavage ...
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bioRxiv - Cancer Biology 2022Quote: ... rLys-C (Promega) was added at 1:50 weight:weight to the S-Trap column ...
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bioRxiv - Systems Biology 2022Quote: ... Glu-C (Promega) or Lys-C (Wako chemicals ...
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bioRxiv - Pathology 2024Quote: ... Lys-C (Promega) was added in an enzyme:substrate ratio of 1:75 for 4 hours at 25 °C ...
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bioRxiv - Bioengineering 2024Quote: ... Lys-C (Promega) was added at a ratio of enzyme:protein of 1:70 (w/w) ...
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bioRxiv - Developmental Biology 2020Quote: ... Required fragments were PCR-amplified from wild-type genomic DNA and used as template for preparation of DIG-labeled probe using T7 RNA Polymerase (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: MAO B catalytic activity was determined by MAO-Glo Assay (Promega). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... DIG-labeled anti-sense probes (cmyb, spi1, lcp1, and hbae1) were made from the pGEM®-T Easy vector (Promega Corporation, A1360) containing the gene-coding sequences via in vitro transcription using DIG RNA Labeling Kit (Roche ...
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bioRxiv - Cancer Biology 2024Quote: LC-MS/MS-based mono-methylated/dimethylated-peptide analyses: NADK purified from HEK293T cells was digested with Glc-C and Lys-C mixture (sequencing grade, Promega, protein: Glc-C: Lys-C = 20:1:1) at 37°C for 16 h and desalted using C18 Zip-Tips (Millipore).
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bioRxiv - Neuroscience 2022Quote: ... and digested overnight at 37 °C with Trypsin/Lys-C (Promega, V5071) at a ratio of 1/50 ...
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bioRxiv - Biochemistry 2023Quote: ... overnight at 37°C or sequentially with trypsin and Glu-C (Promega) at an enzyme-to-substrate ratio of 1:20 and 1:50 at 37°C and 25°C ...
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bioRxiv - Cell Biology 2020Quote: ... or Lys-C (Promega). Proteins were resuspended in 100 µl of the appropriate digestion buffer (trypsin ...
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bioRxiv - Developmental Biology 2020Quote: ... Endoproteinase Lys-C (Promega), 5 μL at 1 μg/μL ...
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Multi cell line analysis of lysosomal proteomes reveals unique features and novel lysosomal proteinsbioRxiv - Molecular Biology 2020Quote: ... rLys-C (Promega, V1671) added at an enzyme to protein ratio of 1 to 100 (w/w) ...
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bioRxiv - Biochemistry 2021Quote: ... or Lys-C (Promega) at 37 °C for 12 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Trypsin/Lys-C (Promega) solution was added and incubated at 37 °C for 12 h ...
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bioRxiv - Microbiology 2020Quote: ... and Glu-C (Promega). Reaction mixtures were dried and peptides/glycopeptides were extracted using C18 Zip-tip (MerckMilipore ...