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Citations for Promega :
351 - 400 of 3082 citations for Dengue Virus serotype 1 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... PHH were infected with HepAD38-derived GTD virus at 500 viral genome equivalents per cell in maintenance medium supplemented with 4 % PEG 8000 (Promega, Madison, WI; V3011). For patient sera infections ...
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bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
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bioRxiv - Genomics 2020Quote: ... Five micrograms of RNA was reverse transcribed into cDNA using Moloney-murine leukemia virus reverse transcriptase and random primers (Promega, Madison, Wisconsin, USA) in a 20 μl reaction volume ...
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bioRxiv - Plant Biology 2022Quote: ... Subsequently 2 μg of RNA were used to perform reverse transcription in the presence of Moloney murine leukemia virus reverse transcriptase (Promega, Madison, WI, USA) after annealing with an anchored oligo(dT)18 primer as described by (Wirth et al. ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Microbiology 2020Quote: ... NanoLuc luciferase activity in the lysates was measured using the Nano-Glo Luciferase Assay System (Promega) and a Modulus II Microplate Multimode reader (Turner BioSystem ...
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bioRxiv - Immunology 2021Quote: ... and NanoLuc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units (RLUs ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL of the lysate were added to 50 μL luc substrate (Promega Luciferase Assay System). The amount of luciferase was quantified by luminescence (relative luminescence units (RLU)) ...
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bioRxiv - Developmental Biology 2021Quote: ... and the resulting mRNAs (2 μg) were translated in 50 μl of rabbit reticulocyte lysate (Promega). Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... was in vitro transcribed and translated with a reticulocyte lysate system TnT T7 (Promega, Fitchburg, WI) by incubation of plasmid DNA (1 mg ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets were lysed and bait proteins in the lysates were bound to HaloLink Magnetic Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Gaussia luciferase activity was then measured from 10ul of lysate using the Renilla luciferase kit (Promega) with a FLUOstar Omega plate reader (BMG Labtech) ...
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bioRxiv - Microbiology 2021Quote: ... and Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) and a Glomax Navigator luminometer (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and luciferase activity was measured in cell lysates (Renilla luciferase assay system, Promega Madison WI, USA).
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bioRxiv - Microbiology 2020Quote: ... The lysate was then diluted to <2 M urea and incubated with 50 μg trypsin (Promega) overnight at 37 °C under agitation ...
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bioRxiv - Microbiology 2021Quote: ... Firefly luciferase assays were performed on 20 μL of cell lysates using Luciferase Assay System (Promega) according to the manufactures instructions ...
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bioRxiv - Cell Biology 2021Quote: ... free and total cholesterol concentration in lysates was determined using Cholesterol/Cholesterol Ester-Glo Assay (Promega) following manufacturer recommendations ...
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Metabolic switching and cell wall remodelling of Mycobacterium tuberculosis during bone tuberculosisbioRxiv - Microbiology 2022Quote: ... the cell lysate was pelleted and read for RLU using a luminometer (Promega GloMax 20/20).
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bioRxiv - Molecular Biology 2022Quote: ... 50 μL of the lysate were added to 50 μL luc substrate (Promega Luciferase Assay System). The amount of luciferase was quantified by luminescence (relative luminescence units (RLU)) ...
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bioRxiv - Molecular Biology 2020Quote: Constructs for expression in rabbit reticulocyte lysate (RRL) were based on the SP64 vector (Promega, USA). Constructs for translation in the PURE system were generated from the T7 PURExpress plasmid (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... the luciferase activity in the cell lysates was determined using the Dual Luciferase Reporter System (Promega). Firefly luciferase activities were normalized to that of Renilla luciferase ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were assessed using the Dual-Luciferase Reporter Assay System (#E1910; Promega, Madison, WI, USA). Luminescence was measured using a TECAN M200 infinite-PRO plate reader (Tecan ...
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bioRxiv - Microbiology 2021Quote: ... Luminescence activity in the resulting cell lysates was measured using the Luciferase Assay System (Promega, USA).
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bioRxiv - Molecular Biology 2019Quote: ... and lysates were assayed for dual luciferase activity using the Dual Luciferase Reporter Assay System (Promega) and a luminometer ...
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bioRxiv - Immunology 2021Quote: ... The luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega) and GloMax system (Turner BioSystems) ...
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bioRxiv - Genomics 2020Quote: DNA extraction was performed on the phage lysate using the Wizard DNA Clean-Up kit (Promega) with minor modifications ...
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bioRxiv - Immunology 2020Quote: ... and NanoLuc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units (RLUs ...
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bioRxiv - Developmental Biology 2020Quote: Unlabelled in vitro translated protein was produced using rabbit reticulocyte lysates according to manufacturer’s protocol (Promega).
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bioRxiv - Immunology 2021Quote: ... Luciferase activity in total cell lysates was measured using a dual-luciferase reporter assay system (Promega).
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bioRxiv - Cell Biology 2022Quote: Constructs for expression in rabbit reticulocyte lysate (RRL) used the SP64 vector as a backbone (Promega). For in vitro insertion reactions ...
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bioRxiv - Genetics 2022Quote: ... The lysate was digested overnight at 37 °C with 2 μg of sequencing-grade trypsin (Promega), after which the sample was acidified to 0.1% formic acid ...
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bioRxiv - Systems Biology 2022Quote: ... In-solution digestions were performed on lysates containing 5 mg of protein using either Trypsin (Promega), Chymotrypsin (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... The mRNA obtained was used for in vitro translation in Flexi Rabbit Reticulocyte Lysate System (Promega) and the resulting lysate was directly used in the import reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were used to measure Luciferase activities with the Dual-Luciferase Reporter Assay System (Promega) as indicated by the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Nanoluc Luciferase activity of lysates was then measured using the Nano-Glo Luciferase Assay System (Promega) with GloMax Discover System reader (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... Then 50 µL of lysates were mixed to 50 µl D-luciferine (Luciferase assay system, Promega) and the Firefly luciferase activity was determined for each cell lysate with an Infinite 200 Pro (Tecan ...
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bioRxiv - Microbiology 2023Quote: ... 20 μL of lysate was incubated at room temperature with 20 μL of BrightGlo reagent (Promega) for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 2μl of the lysate was subsequently used in a 20μL PCR reaction (Promega, Madison WI, USA) with forward primer 342F (5’-CTA CGG GGG GCA GCA G -3’ ...
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bioRxiv - Immunology 2022Quote: ... Nanoluc Luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with Modulus II Microplate Reader User interface (TURNER BioSystems) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and equal amounts of lysates were used for luciferase reporter assay following the manufacturer’s instructions (Promega).
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bioRxiv - Developmental Biology 2023Quote: ... Luciferase activity in the cell lysates was measured using a Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... NanoLuc luciferase activity in the lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with a Tecan Infinite microplate reader ...
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bioRxiv - Immunology 2023Quote: ... The luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega) and GloMax system (Turner BioSystems) ...
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bioRxiv - Cell Biology 2023Quote: ... 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega). Translation reaction products from 0.25 μg of plasmid DNA were incubated with GST-labeled proteins on glutathione-Sepharose beads in NETN-E buffer (50 mM Tris ...
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bioRxiv - Bioengineering 2023Quote: ... Luciferase signals from the cell lysate were evaluated using the GloMax 96 Microplate Luminometer (Promega, USA).
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bioRxiv - Immunology 2024Quote: ... Luciferase activity was measured in purified CD8 T cell lysates with the luciferase assay system (Promega). Purified CD8 cells were lysed using cell lysis buffer 1X ...
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bioRxiv - Biophysics 2021Quote: ... and were produced by translation in rabbit reticulocyte lysates using TnT Quick Coupled Transcription/Translation System (Promega) (see Supplementary Table 5 for plasmids) ...
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bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity of the lysates was measured using a Nano-Glo Dual-Luciferase Reporter Assay System (Promega) and SpectraMax Paradigm (Molecular Devices ...
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bioRxiv - Molecular Biology 2021Quote: Messenger RNAs were translated in nuclease-treated rabbit reticulocyte lysate (RRL) or wheat germ (WG) extracts (Promega). Typical reactions were composed of 90% v/v RRL ...
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bioRxiv - Cancer Biology 2019Quote: ... Firefly luciferase activity present in cellular lysates were assayed using the dual luciferase reporter system (Promega Corp.), and light emission was quantified using a luminometer (Junior Berlthold).