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Citations for Promega :
51 - 100 of 2892 citations for Dengue Virus VLP Serotypes 1 4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) system for RT-PCR (Promega) was used for cDNA synthesis using 500 ng extracted RNA from all samples ...
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bioRxiv - Molecular Biology 2024Quote: ... An equal amount of extension mix consisting of avian myeloblastosis virus reverse transcriptase (Promega), 5x AMV buffer and 40 μM dNTPs was added ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized with Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI). qRT-PCR was performed with the BioRad CFX96 Real-Time PCR System or Applied Biosystems 7000 ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µl of RNAse A solution (4 mg ml-1) (Promega) were added before incubation at 37°C for 15 min ...
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bioRxiv - Biochemistry 2020Quote: ... Complementary DNA was reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega, Wisconsin, USA). Relative expression of genes of interest were quantified by real-time PCR using TaqMan™ gene expression assays (Mm02619580_g1 ACTB ...
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bioRxiv - Plant Biology 2021Quote: ... and 2μg RNA were transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). A 20-fold dilution of the cDNA reaction was used for PCR amplification with the ABsolute QPCR SYBR Green Capillary Mix (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was produced with Moloney murine leukemia virus reverse transcriptase (M-MLV RT) (Promega) and random hexamers ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... RNA was then reverse transcribed using Moloney-Murine Leukaemia Virus (M-MLV) reverse transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and DNase-I digested before murine leukemia virus (MLV) reverse transcription using random primers (Promega). qPCR was performed using the TaqMan technology (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... and pVSV-G (4:3:1 ratio) using calcium phosphate transfection (Promega) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Viral RNA was extracted from virus-infected culture medium by using Maxwell RSC Instrument (Promega, AS4500). cDNA preparation and amplification were done in accordance with protocols published by the ARTIC network (https://artic.network/ncov-2019 ...
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bioRxiv - Molecular Biology 2021Quote: ... Renilla luciferase is driven by a constitutively active simian virus 40 (SV40) promoter (pRL-SV40; Promega), which served as a control for cell viability and transfection efficiency ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Virus infectivity was assayed by measuring chemiluminescent firefly luciferase activity using the commercial kit from Promega.
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bioRxiv - Microbiology 2021Quote: All virus stocks were produced by plasmid transfection of HEK 293T cells with Fugene 6 (Promega). Supernatants were harvested at 48h and 72h ...
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bioRxiv - Cell Biology 2021Quote: ... as much liquid was removed as possible and HaloTEV (Tobacco Etch Virus) protease (300 units, Promega) was added to liberate protein ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Microbiology 2020Quote: The total RNA was reverse transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). Real-time PCR was performed using the POWER SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... Reverse transcription (RT) was carried out using 2.5 U avian myeloblastosis virus (AMV) reverse transcriptase (Promega) and a 32P-labelled primer in the presence of 4 mM MgCl2 and 0.5 mM dNTPs ...
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bioRxiv - Microbiology 2020Quote: ... Cells transduced with luciferase expressing virus were assessed using Promega Steady-Glo system (Promega Madison, WI)
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was then performed with 50 U of Avian Myeloblastosis Virus reverse transcriptase (Promega Corporation) for 20 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: Reverse transcription was performed using the Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, Madison, WI, USA), 1000 ng of total RNA and random hexamers ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped virus were prepared by transfection of HEK293T cells using the Fugene HD transfection system (Promega), as previously described(42) ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM CaCl2) and incubated for 4 hours with 1 µg trypsin (Promega) at 37°C and shaking at 1200 rpm ...
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bioRxiv - Immunology 2021Quote: ... and reversed transcribed with random hexamers (IDT) using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... Virus -specific PCR products were cloned into pGEM-T Easy Vector (A1360, Promega Co., Madison, WI, USA) and used to transform E ...
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using Moloney Murine Leukemia Virus Reverse Transcriptase (MML-V)(Promega Corporation, Madison, WI). ViiA-7 instrument (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... Reverse transcription was carried out using moloney-murine leukemia virus (M-MLV) reverse transcriptase kit (Promega, USA). Reverse transcription was completed in a 30 µL final volume reaction containing 400 U M-MLV and 1 mM dNTP mix (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... overnight at 4°C and HRP-conjugated anti-rabbit antibody (1:2,000, W4011, Promega) for 1 hr at room temperature followed by ECL signal amplification and chemiluminescence detection (SuperSignal West Pico Chemiluminescent Substrate ...
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bioRxiv - Systems Biology 2023Quote: ... for 4 h at 37 °C and then with trypsin (Promega, 1:100, w:w) overnight at 37 °C ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesized using Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and universal Random hexamers (Promega). qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of different HCV genomic regions was performed using avian myeloblastosis virus (AMV) reverse transcriptase (Promega), and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... A total of 1.5 μg RNA was reversely transcribed using Moloney murine leukemia virus reverse transcriptase (M1701, Promega) for 90 min at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized using M-MLV (Moloney murine leukemia virus) reverse transcriptase (Promega, Madison, WI, USA), and amplified using SYBR Green qRT-PCR SuperMix-UDG reagents (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... virus-containing media was removed and replaced with 35 μl of 1x Passive lysis buffer (Promega; Ref# E194A). Plates underwent three freeze-thaw cycles consisting of freezing on dry-ice for 15 minutes followed by thawing at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... virus inoculum was removed and cells were then transfected with 2 µg of plasmid DNA using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... The reverse transcription (RT) mix contained 200 units of recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Promega), 20 units of RNAsin (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... reverse transcription was performed with 0.5 μl (corresponding to 5 U) of Avian Myeloblastosis Virus Reverse Transcriptase (Promega), 0.1 μl dNTP mix (10 mM) ...
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bioRxiv - Immunology 2023Quote: ... 2 μg RNA was used to synthesize first-strand cDNA using Moloney mouse leukemia virus (M-MLV) (Promega). The amplification of specific PCR products was detected using SYBR Premix Ex TaqTM II (Vazyme) ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 1.5 µg RNA was reversely transcribed using 0.5 μl Moloney murine leukemia virus reverse transcriptase (M1701, Promega), 4 µl RT buffer 5x (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were collected at 48 h post-infection and virus infectivity were measured by luciferase assay (Promega).
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bioRxiv - Cell Biology 2019Quote: ... The reaction was diluted 1:4 with 50 mM ammonium bicarbonate and incubated with 1 µg of Trypsin Gold (Promega) at 37°C for more than 18 h before desalting ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then incubated overnight at 4 °C with anti-p75NTR (Promega; G323A; 1:300) and anti-TRADD (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... Neurons were then incubated overnight at 4° C with anti-p75NTR (Promega; G323A; 1:500) and anti-TRAF6 (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: ... 4°C) and then resuspended in 100 µl 1 x Passive Lysis Buffer (PLB, Promega). 45 µl of the luciferase assay substrate dissolved in Luciferase Assay Buffer II was plated on a 96-well plate and 10 µl of the cell lysate was added immediately before measurement of Fluc activity using a Tecan reader ...
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bioRxiv - Molecular Biology 2022Quote: ... Compound treatment was initiated 1-4 hours before transfections with FuGENE HD transfection reagent (Promega) following the manual ...
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bioRxiv - Plant Biology 2020Quote: ... DNA-free RNA (500 µg) was used for reverse transcription using the Moloney Murine Leukemia Virus Reverse transcriptase (Promega, Madison ...
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bioRxiv - Microbiology 2020Quote: ... Two hundred nanograms of purified RNA were reverse-transcribed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT, Promega). RT was primed with oligo dT(15 ...