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Citations for Promega :
701 - 750 of 1641 citations for Dengue Virus Serotype 4 NS1 Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The proteins were digested with Trypsin/LysC mix (Promega, Wisconsin, WI, USA) at a 1:50 (w/w ...
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bioRxiv - Immunology 2023Quote: ... Protein was digested with either trypsin or chymotrypsin (Mass Spectrometry Grade, Promega) at a mass ratio of 1:30 overnight at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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bioRxiv - Biochemistry 2023Quote: ... A whole-cell protein extract of Saccharomyces cerevisiae (Promega, V7341, lot 434786) was deployed ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were digested in-gel using trypsin (600 ng/band, Gold, Promega), as previously described [29] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HiBit-tagged proteins were detected using the NanoGlo HiBit Blotting kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Treated proteins were then digested with sequencing grade modified trypsin (Promega, V5111) and incubated overnight (37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... The TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega, L3260) was used to generate Halo-tagged proteins starting with pIX-HALO plasmids as described previously (Mukherjee et al. ...
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bioRxiv - Plant Biology 2023Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA). Each sample was further analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... proteins isolated by gel electrophoresis were digested with trypsin (Promega, Wisconsin, USA) in 100 mM NH4HCO3 (pH 8.0) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The proteins were subsequently digested overnight with molecular grade trypsin enzyme (Promega) using a ratio of 1:50µg (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with 0.2 μg trypsin (Promega). Formic acid was then added to 2% and the samples were incubated at 37°C for 30 min to cleave RapiGest ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified proteins were digested with sequencing-grade trypsin (Promega, Madison, Wisconsin, USA) and separated by nanoAcquity UPLC (Waters ...
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bioRxiv - Neuroscience 2024Quote: ... and digested with trypsin (1:50 enzyme: protein ratio; Promega, United States) at 37°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were on-bead digested using 500 ng Sequencing Grade Trypsin (Promega). The digestion was carried out at 37°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... subjected to protein precipitation using Chloroform/Methanol and digested with trypsin (Promega). Desalted and acidified peptides (0.1% TFA ...
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bioRxiv - Cancer Biology 2024Quote: ... Clarified lysates were quantified using the Pierce TM BCA protein assay (Promega) and boiled in 1x sample buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were digested using trypsin/Lys-C mixture (Mass Spec Grade, Promega) for 16 h according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2024Quote: ... The protein-bead complexes were digested with a mixture of trypsin:LysC (Promega) at a 1:20 ratio overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... before proteolytic digestion with LysC (enzyme to protein ratio 1:100, Promega) for 3 h and with trypsin (1:25 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were pulsed for 4 h with CellTiter 96 Aqueous One solution reagent (Promega). Cell viability was measured by a luminometer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... after which growth media was removed and 20 µl of 4% D-luciferin (Promega) in CO2 independent medium (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... overnight at 4°C and HRP-conjugated anti-rabbit antibody (1:2,000, W4011, Promega) for 1 hr at room temperature followed by ECL signal amplification and chemiluminescence detection (SuperSignal West Pico Chemiluminescent Substrate ...
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bioRxiv - Biochemistry 2022Quote: 4 × 105 HEK293T cells were lysed in 100 μl of Passive Lysis Buffer (Promega). 10 μl of cell lysates were separated on a NuPAGE 4-12% Bis-Tris polyacrylamide gel (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... and then ligated for 16 h at 4 °C with T4 DNA ligase (Promega). After a further purification step ...
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bioRxiv - Biochemistry 2019Quote: 4 x 105 HEK293T cells were lysed in 100 μl Passive Lysis Buffer (Promega). 10 μl of cell lysates were boiled with 2.5 μl 4x gel loading buffer for 5 min at 98°C and run on a NuPAGE 4-12% Bis-Tris polyacrylamide gel for 45 min at 200 V ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromosome 4: 101,716,750-101,718,250 forward strand) was cloned into pGL3-basic vector (E1751, Promega). Reporter vector ...
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bioRxiv - Systems Biology 2023Quote: ... for 4 h at 37 °C and then with trypsin (Promega, 1:100, w:w) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... synthesized from Gene Universal (USA) at 4°C overnight (T4 DNA ligase, Promega, USA). Cys-loop mutation ligation products were transformed into DH5α (ThermoScientific ...
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bioRxiv - Microbiology 2024Quote: ... pH 8.0 before adding 4 µL of 0.5 µg/µL sequencing grade trypsin (Promega) and incubated over night at 37°C ...
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bioRxiv - Genetics 2024Quote: ... transfected with 4 µg of plasmid DNA using the FuGENE® HD Transfection (Promega) for 24 h ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Cell Biology 2020Quote: ... Radiolabelled protein was translated from mRNA using the rabbit reticulocyte lysate system (Promega) and 35S-labelled methionine ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was digested for overnight with 5 ng/µL trypsin (Promega Corporation) in 50 mM ammonium bicarbonate at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... The construct expresses rat GR fused with HaloTag protein (Promega, Madison, WI, USA) in the C-terminal domain under the CMVd1 promoter ...
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bioRxiv - Developmental Biology 2020Quote: Proteins were digested on-bead with 0.5 ug Sequencing Grade Modified Trypsin (Promega) overnight at 37°C with agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA).
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bioRxiv - Neuroscience 2021Quote: ... The proteins were digested with 2.5 μg of sequencing grade modified trypsin (Promega). The resulting peptides purified with C18 MicroSpin columns (The Nest Group ...
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bioRxiv - Cell Biology 2019Quote: ... the bead-bound protein was incubated with either 6.7 µM HaloTag-AlexaFluor660 (Promega) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were digested with 2 μg of trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... proteins from HL-1 cells were extracted with Passive Lysis Buffer (Promega France). Quantification of bioluminescence was performed with a luminometer (Centro LB960 ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were digested by adding sequencing grade trypsin (1:20 substrate:enzyme; Promega #5111) and incubating at 37°C for 16 h ...
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bioRxiv - Cell Biology 2020Quote: ... before analysis of luciferase protein levels using Dual-Glo luciferase assay system (Promega) and an EnSpire multimode plate reader (Perkin-Elmer).
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bioRxiv - Systems Biology 2020Quote: The total resultant protein content was digested with trypsin (Trypsin Gold, Promega, USA). The enzyme was added at the ratio of 1:40 (w/w ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were directly digested on the beads by trypsin (Promega, Madison, WI, USA) overnight at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
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S-nitrosoglutathione reductase deficiency causes aberrant placental S-nitrosylation and preeclampsiabioRxiv - Physiology 2021Quote: ... 500 μg was digested overnight using 0.02 μg trypsin/μg of protein (Promega). iodoTMT6 labeled samples were combined according to the label’s isotope ...
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bioRxiv - Plant Biology 2021Quote: ... Protein production was confirmed by western blot using an anti-HaloTag antibody (Promega). HaloTag-ligand conjugated magnetic beads (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... One hundred μg of protein from each sample were digested with trypsin (Promega) at 37°C for 24 hours with an enzyme to protein ratio 1:100 (w/w) ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested with 0.4 µg of sequencing grade modified trypsin (Promega, #V5111) in 40 µl Hepes/NaOH ...
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bioRxiv - Plant Biology 2021Quote: ... The fusion proteins were purified using the MagneGST™ Pull-Down System (Promega). The subsequent EMSAs were performed using a LightShiftTM Chemiluminescent EMSA Kit (Thermo Fisher ...