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Citations for Promega :
1 - 50 of 954 citations for Dengue Virus Serotype 2 VLP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... VLP were treated with 20 units/mL RQ1-DNase (Promega) in 10mM MgCl2 for 1h at 37°C before infection ...
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bioRxiv - Microbiology 2020Quote: ... We replaced the mCherry reading frame with nano-luciferase (nLuc-VLP) (Promega) or codon optimized GFP (GFP-VLP) ...
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bioRxiv - Microbiology 2021Quote: RSV VLPs were produced by FuGENE® 6 Transfection Reagent (Promega, E2691) transfection of DF1s at 50% confluence with 1 µg of viral plasmid ...
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bioRxiv - Genetics 2024Quote: ... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
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bioRxiv - Microbiology 2020Quote: ... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Immunology 2020Quote: All VLPs were produced by transient transfection of HEK293T cells with Fugene 6 (Promega, ref E2691). HEK293T were seeded in 15-cm dishes to reach 60-70% confluency the next day and VLPs were produced by co-transfecting plasmids encoding Gag-eGFP and the VSV-G envelope (pGag-EGFP and pCMV-VSV-G ...
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bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... virus inoculum was removed and cells were then transfected with 2 µg of plasmid DNA using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... 2 μg RNA was used to synthesize first-strand cDNA using Moloney mouse leukemia virus (M-MLV) (Promega). The amplification of specific PCR products was detected using SYBR Premix Ex TaqTM II (Vazyme) ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Biophysics 2023Quote: ... equal volumes of HiBiT-N VLPs and hACE2-LgBiT EVs were mixed with nanoluc substrate (cat#N2420, Promega) and trypsin (Sigma ...
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bioRxiv - Microbiology 2023Quote: Cells infected with a luciferase-encoding virus were lysed 2 days after infection with a Bright-Glo Luciferase Assay System (E2620, Promega) and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing the GFP control or targeting sgRNAs were generated by transfection of HEK293 cells with FuGene (Promega). VLPs were collected from culture supernatant ...
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bioRxiv - Biophysics 2020Quote: ... Virus infectivity was determined by luciferase production (Promega) after 48h using a Synergy2 Multi-Detection Microplate Reader (BioTek)
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bioRxiv - Microbiology 2020Quote: ... virus replication was measured by nLUC assay (Promega) using a SpectraMax plate reader (Molecular Devices).
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bioRxiv - Immunology 2020Quote: ... using Moloney murine leukemia virus reverse transcriptase (Promega) according to manufacturers’ instructions ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Cell Biology 2021Quote: ... conducted with Moloney murine leukemia virus reverse transcriptase (Promega) and random hexamers (Roche Applied Science ...
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bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus MMLV reverse transcriptase (Promega) was performed according to the vendor’s protocol.
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bioRxiv - Bioengineering 2022Quote: ... Virus growth was measured with NanoGlo Luciferase Assay System (Promega) and cell viability was measured with CellTiter-Glo 2.0 Cell Viability Assay (Promega) ...
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bioRxiv - Microbiology 2019Quote: ... 200 U of Moloney murine leukemia virus reverse transcriptase (MMLV; Promega) in 30 mM Tris and 20 mM KCl (pH 8⋅3 ...
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bioRxiv - Cell Biology 2020Quote: ... Virus DNA liposomes were prepared using FuGENE HD Transfection reagent (Promega) and DMEM ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 unit/μl Avian Myoblastosis virus reverse transcriptase (Promega®) were added and incubated for 1h at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... Virus infectivity was measured by β-galactosidase activity (Promega, Madison, WI). Virus stocks were concentrated (20X ...
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bioRxiv - Microbiology 2023Quote: Virus neutralization was measured using a β-galactosidase-based assay (Promega) with TZM.bl target cells (85) ...
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bioRxiv - Cell Biology 2023Quote: ... and reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega). cDNA was amplified in a thermal cycler (GeneAmp PCR System 9700 ...
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bioRxiv - Cancer Biology 2024Quote: ... Moloney Murine Leukemia Virus Reverse Transcriptase RNase H-Point Mutant (Promega) was used for cDNA synthesis ...
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bioRxiv - Molecular Biology 2019Quote: ... and cDNA was generated using the Moloney Leukemia Virus Reverse Transcriptase (Promega), both according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2019Quote: ... and reverse transcribed using Moloney murine leukaemia virus reverse transcriptase enzyme (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... virus stocks were first treated with 50 units/ml RQ1-DNase (Promega) for 1 h to remove any plasmid DNA ...
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bioRxiv - Molecular Biology 2019Quote: ... and reverse transcribed with Moloney murine leukemia virus reverse transcriptase RNase H(−) (Promega) using oligoT primer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each RNA sample was reverse transcribed using murine leukemia virus reverse transcriptase (Promega) and random DNA hexamer primers ...
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bioRxiv - Microbiology 2020Quote: ... Cells infected with Luciferase contaning virus were analyzed by Bright-Glo™ (Promega) using the manufacturers protocol ...
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bioRxiv - Immunology 2019Quote: ... cDNA was generated using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega) with random hexamer primers ...
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bioRxiv - Molecular Biology 2021Quote: ... and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Promega, Madison, WI), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus M-MLV reverse transcriptase (Promega, Madison, WI, USA) was performed according to the vendor’s protocol ...
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bioRxiv - Microbiology 2019Quote: Virus infectivity and neutralization was measured using a β-galactosidase-based luciferase assay (Promega) with TZM-bl target cells ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNAs were synthesised using Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, www.promega.com). The quantification of gene expression was made by qRT-PCR ...
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bioRxiv - Genetics 2020Quote: ... Total RNA (0.5 µg) was reverse transcribed with mouse myeloblastosis virus reverse transcriptase (Promega) under standard conditions using hexanucleotide random primers according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and then used for reverse transcription with avian myeloblastosis virus (AMV) reverse transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The virus-induced CPE was assessed using the Viral ToxGlo™ assay (Promega #G8943) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) system for RT-PCR (Promega) was used for cDNA synthesis using 500 ng extracted RNA from all samples ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized with Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI). qRT-PCR was performed with the BioRad CFX96 Real-Time PCR System or Applied Biosystems 7000 ...
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bioRxiv - Molecular Biology 2024Quote: ... An equal amount of extension mix consisting of avian myeloblastosis virus reverse transcriptase (Promega), 5x AMV buffer and 40 μM dNTPs was added ...
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bioRxiv - Biochemistry 2020Quote: ... Complementary DNA was reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega, Wisconsin, USA). Relative expression of genes of interest were quantified by real-time PCR using TaqMan™ gene expression assays (Mm02619580_g1 ACTB ...
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bioRxiv - Plant Biology 2021Quote: ... and 2μg RNA were transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). A 20-fold dilution of the cDNA reaction was used for PCR amplification with the ABsolute QPCR SYBR Green Capillary Mix (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was produced with Moloney murine leukemia virus reverse transcriptase (M-MLV RT) (Promega) and random hexamers ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... RNA was then reverse transcribed using Moloney-Murine Leukaemia Virus (M-MLV) reverse transcriptase (Promega) according to the manufacturer’s instructions ...