Labshake search
Citations for Promega :
1 - 50 of 1250 citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... LgBiT subunit (Promega) was fused at the C-terminus of PTH2R connected by a 20-amino acid linker ...
-
bioRxiv - Molecular Biology 2022Quote: ... LgBiT subunit (Promega) was fused at the C terminus of RXFP4 connected by a 15-amino acid polypeptide linker ...
-
bioRxiv - Biochemistry 2021Quote: ... in which dissociation of a Gα subunit from a Gβγ subunit was monitored by a NanoBiT system (Promega). Specifically ...
-
bioRxiv - Molecular Biology 2022Quote: ... 50 ng/well of chaperone RTP1S,105 50 ng/well of the G protein subunit Gαolf,106–107 olfactory G protein subunit Gγ13,108 and 50 ng/well of pGloSensorTM-22F (Promega, Madison, USA).109 The utilized pGloSensor™-22F is a genetically engineered luciferase with a cAMP-binding pocket ...
-
bioRxiv - Biophysics 2024Quote: ... in which the interaction between a Gα subunit and a Gβγ subunit was monitored by the NanoBiT system (Promega). Specifically ...
-
bioRxiv - Cell Biology 2023Quote: ... various concentrations of the compounds were mixed with 1 - 2 nM sensor and 1,000-fold diluted furimazine (Nano-Glo Luciferase Assay Substrate, Promega) in 100 μL of buffer (50 mM HEPES ...
-
bioRxiv - Biochemistry 2022Quote: ... in which the interaction between the Gα subunit and the Gγ subunit was monitored by the NanoBiT-based enzyme complementation system (Promega). Specifically ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... 50 ng/well of pGloSensorTM-22F cAMP sensor plasmid (Promega), 50 ng/well of the 3x HA MC4R plasmid and 50 ng/well pcDNA3.1 or MRAP2-FLAG were added to 25 mL/well OptiMEM® media ...
-
bioRxiv - Biochemistry 2022Quote: ... the above PTH1R construct was added the LgBiT subunit (Promega) at the C terminus of PTH1R ...
-
bioRxiv - Biochemistry 2023Quote: ... the above PTH1R construct was added the LgBiT subunit (Promega) at the C terminus of PTH1R with a 17-amino acid linker ...
-
bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
-
bioRxiv - Biophysics 2021Quote: ... the NanoBiT tethering strategy was applied by fusing a LgBiT subunit (Promega) at the receptor C-terminus using homologous recombination (CloneExpress One Step Cloning Kit ...
-
bioRxiv - Biochemistry 2022Quote: ... Cytosolic lactate dehydrogenase release was detected using the cytoTox 96® non-radioactive cytotoxicity Assay kit (Promega) as per manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... pCalpain-sensor transfected in PC cells using Fugene HD according to the manufacturer’s instruction (Promega). Donor and acceptor bleed through were corrected using eCFP and eYFP only fluorophores ...
-
bioRxiv - Biophysics 2023Quote: ... the NF-κB subunit p65 (pci-Neo-p65 HaloTag)49 and HaloTag itself (pHTN, Promega).
-
bioRxiv - Biochemistry 2024Quote: ... bacmid DNA containing all subunits was transfected into Sf9 cells using FuGENE® HD (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plasmids encoding the cAMP sensor (pGloSensor-22F) and the following luciferase reporters were purchased from Promega: CRE-luc2 ...
-
bioRxiv - Immunology 2021Quote: ... cells were transfected with the plasmid of the required sensor using FuGene HD transfection reagent (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plasmids encoding the cAMP sensor (pGloSensor™-22F) and CRE-Nluc reporter were purchased from Promega. pFL-tk encoding firefly luciferase under the control of constitutively active thymidine kinase promoter was used as a normalizer for reporter assays ...
-
bioRxiv - Immunology 2022Quote: The ADCC or cell killing activity of effector cells incubated with anti-NKG2A antibodies with LALA-PG mutation were measured through release of cytosolic LDH from dead target cells using LDH-Glo cytotoxicity assay kit (Promega, J2381). The purified NK cells from normal PBMCs (Zenbio Inc. ...
-
bioRxiv - Biochemistry 2020Quote: ... bacmid DNA containing all four subunits was transfected into Sf9 cells using FuGENE® HD (Promega) per manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: Cytotoxicity was evaluated by release of the cytosolic enzyme LDH into the culture medium by dead and dying cells (CytoTox-96 LDH assay; Promega, Southampton, UK). Total LDH release was calculated by incubating untreated cells with 0.1% Triton X-100 for 10 min (37°C ...
-
bioRxiv - Genomics 2024Quote: ... We amplified the Cytochrome c oxidase subunit I (COI) for individual abdomen DNA using the GoTaq (Promega) protocol ...
-
bioRxiv - Immunology 2022Quote: ... The constructs encoding EROS/CYBC1 or gp91phox/CYBB fused to the reporter subunits were custom made by Promega and consist of the following ...
-
bioRxiv - Biochemistry 2022Quote: ... Rat Gβ1 with an N-terminal MHHHHHHSSGLVPRGSHMASHHHHHHHHHH-tag (His16) was fused with the SmBiT subunit (peptide 86, Promega)20 via a 15 amino acid (GSSGGGGSGGGGSSG ...
-
bioRxiv - Plant Biology 2022Quote: ... for 2 h as primary antibody and peroxidase-conjugated goat anti-rabbit antibody (1:10,000; Promega) for 1 h with 5 times 10 min washes in-between the incubations ...
-
bioRxiv - Bioengineering 2022Quote: ... various concentrations of the compounds were mixed with 1 nM sensor and 1,000-fold diluted furimazine (Nano-Glo Luciferase Assay Substrate, Promega) in 100 μL of buffer (50 mM HEPES ...
-
bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...
-
bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
-
bioRxiv - Neuroscience 2024Quote: ... then transfected with pRK5-based constructs of iGluR subunits and Neto variants (1 µg total DNA/ ml cells) using ViaFect transfection reagent (Promega). The cells were incubated at 37°C for 3 hours ...
-
bioRxiv - Biochemistry 2021Quote: ... HCT116 cells were plated on poly-L-lysine coated cover slips and transfected with ER calcium sensor ER-GCaMP6-150 using FuGENE HD (Promega Corp.). Experiments were carried out 1-2 days after transfection ...
-
bioRxiv - Physiology 2022Quote: ... 1.15 μg of purified AQP2 or AQP2 S256A COOH-tail peptide were mixed with either RSK (16 nmol) or PKA catalytic subunit (5.6 nmol) (Promega, Madison, WI) in a total volume of 12 μl reaction buffer containing 40 mM Tris pH 7.5 ...
-
Measuring adaptation dynamics to hydrogen peroxide in single human cells using fluorescent reportersbioRxiv - Cell Biology 2020Quote: ... Wild-type MCF7 cells (MCF7 WT) were transfected with a plasmid expressing the sensor of interest using FuGENE HD transfection reagent (Promega, Charbonnières, France) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... stable cell line expressing the human MC4-R and a split luciferase cAMP genetic sensor based on the circularized fusion of firefly luciferase and protein kinase A regulatory cAMP binding domain (clone 22F, Promega, Madison WI) was used to determine the α-MSH-elicited cAMP responses in live cells as previously described 32 ...
-
bioRxiv - Cell Biology 2024Quote: ... secondary antibodies for 2 hours prior to imaging by enhanced chemiluminescence (Promega ECL) and autoradiography films (GE Healthcare ...
-
bioRxiv - Systems Biology 2023Quote: ... The panel of ssDNA-labelled antibodies (250 ng/ml for each antibody) was mixed with 2 U/µl RNAsin Plus (Promega) and 0.1% Triton X-100 in PBS:PFBB (1:1 ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were rinsed 5 times for 8 min in PBS-tween 0.2% and incubated with secondary antibodies for 2 h at room temperature under agitation (HRP anti-mouse, 1:10000, Promega cat. # W4021). Membranes were further rinsed 5 times for 8 min in PBS-tween 0.2% ...
-
bioRxiv - Immunology 2024Quote: ... Blots were incubated with primary antibodies overnight at 4°C followed by 2-hour incubation with HRP-conjugated secondary anti-rabbit IgG-HRP conjugate antibodies (1:10,000; Promega W401B) at room temperature ...
-
bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:5000 in 2% BSA in TBST) for 1 h at room temperature followed by development in ECL (Thermo Fisher Scientific) ...
-
bioRxiv - Bioengineering 2024Quote: One hundred micrograms of each antibody were reduced with 50 mM DTT and treated with 2 µl PNGase F (Promega, USA) at 37°C for over 16h with stirring before being transferred to a new vial for LC-MS analysis ...
-
bioRxiv - Microbiology 2021Quote: ... 2) Thermolysin (Promega) or 3 ...
-
bioRxiv - Microbiology 2021Quote: ... (2) Trypsin (Promega), 4 h ...
-
bioRxiv - Cell Biology 2022Quote: ... Erk1/2 (Promega), phospho-Erk1/2 (Promega) ...
-
bioRxiv - Cell Biology 2021Quote: The medium in 96-well plate was replaced at the indicated time points with 100 μl of PBS supplemented with 0.2 g/l EDTA and 50 μM FITC-VAD-fmk (Promega, USA) or 50 μM 1,1’,3,3,3’,3’-hexamethylindodicarbo-cyanine iodide (DiIC1(5) ...
-
bioRxiv - Immunology 2024Quote: ... and 2 mg/ml 3-[4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]2-[4-sulfophenyl]-2H-tetrazolium (PMS, Promega) (1/20 ...
-
bioRxiv - Cell Biology 2022Quote: ... were stained with CaspACE FITC-VAD-FMK (fluorescein isothiocyanate-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone) in situ Marker (Promega, Mannheim, Germany) as per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: The increase in caspase like enzymatic activity was evaluated by using a staining solution containing (FITC)-VAD-FMK (CaspACE™, Promega) at a final concentration of 10 µM/ml (74) ...
-
bioRxiv - Immunology 2024Quote: ... Cell viability was assessed by incubating at 37°C up to 107 cells resuspended in complete RPMI medium containing 10 µM of the CaspACE™ FITC-VAD-FMK in Situ Marker (Promega). After 30 min ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 2 μL BSA (1 mg/mL) and 2 μL buffer H (Promega) in reaction volume of 20 μL ...
-
bioRxiv - Microbiology 2020Quote: ... at a ratio of 2:2:1 using Fugene®HD (Promega). At 48 h post transfection ...