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Citations for Promega :
601 - 650 of 4425 citations for Cyclic GMP Direct ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Samples were digested overnight at 37 °C with 5 µg sequencing-grade modified trypsin (Promega). The samples were then acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2020Quote: ... Cells (5 × 106) were then lysed using 100 μl of RIPA lysis buffer (Promega, WI) supplemented with 1 μl of proteinase inhibitor and 10 μl of phenylmethylsulfonylfluoride ...
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bioRxiv - Molecular Biology 2022Quote: ... terminated by incubation on ice and diluted 1:5 in Glo Lysis Buffer (Promega # E2661). 25 μL of prepared Nano-Glo reagent (Promega # N1120 ...
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bioRxiv - Systems Biology 2019Quote: ... Half of the cDNA (5 μl) was amplified by PCR using Pfu Polymerase (Promega, M7745) with the cycling conditions (95°C for 2 min ...
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bioRxiv - Cancer Biology 2019Quote: ... before dilution with 120 μL 0.04 × ProteaseMax and 5 μg/μL sequencing grade trypsin (Promega) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Survival was determined after 5 days of incubation using the Cell Titre-Blue assay (Promega). All cell lines were treated with 4 and 8 µM Erastin and 3 and 6 µM RSL3 ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was incubated at 80°C for 5 min and mixed with FuGENE HD (Promega) in a ratio of 3:1 (FuGENE:DNA ...
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bioRxiv - Plant Biology 2022Quote: ... and 0.3 µl of Taq polymerase (5 u/µl) provided by Promega (Madison, Wisconsin, U.S.A.). Variations in MgCl2 (0.5–1 µl ...
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bioRxiv - Plant Biology 2023Quote: ... 5’RACE products were gel-purified and cloned in pGem®-T Easy vector (Promega). Independent clones were randomly chosen and sequenced by Sanger sequencing method.
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in 400 μL 50 mM NH4HCO3 containing 5 μg modified sequencing grade trypsin (Promega), then transferred to a protein LoBind tube (Eppendorf ...
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bioRxiv - Biochemistry 2023Quote: ... with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer; Promega # E2661) and incubated at room temperature for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μg of the isolated recombinant bacmid and 5 μL Fugene HD transfection reagent (Promega) were diluted in 150 μL Sf-900™ III SFM ...
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bioRxiv - Cell Biology 2023Quote: ... Purified 5 μg of pAd/CMV/V5-DEST plasmid was transfected using Fugene HD (Promega) when the 293A cells were 80% confluent ...
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bioRxiv - Cancer Biology 2024Quote: ... After 5 days cells were collected per manufacturer’s instructions using either CellTiterGlo® (Promega, G7570) to measure viability by assessing ATP concentrations ...
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bioRxiv - Plant Biology 2024Quote: ... 4 hours incubation at 37 °C) and 5 μg of trypsin (Trypsin Gold from Promega, in 50 mM TEAB pH 8.5 ...
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bioRxiv - Biochemistry 2024Quote: ... Luciferase activity was measured 5 h later using Bright-Glo Luciferase Assay System (Promega, E2620) on a Tristar 5 microplate reader (Berthold).
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bioRxiv - Molecular Biology 2024Quote: ... 5–10 embryos were collected at 3 hpf and lysed in Passive Lysis Buffer (Promega) containing cOmplete Protease Inhibitor Cocktail (Sigma‒Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted using a commercial kit (Wizard Genomic DNA Purification Kit, Promega, Madison, WI). Libraries were produced by first fragmenting 75 ng DNA with the Nextera reagent (Illumina ...
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bioRxiv - Zoology 2024Quote: DNA was extracted with several different kits including the Wizard Genomic DNA Purification kit (Promega) and the Qiagen Blood & Tissue kit ...
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bioRxiv - Cell Biology 2019Quote: HeLa cells were cultured on 12-well plates and transfected with the indicated plasmids using Viafect (Promega, Madison, WI). After expression for 24h ...
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bioRxiv - Molecular Biology 2021Quote: ... sphere cells growing in the low attachment plates were quantitated by CellTiter-Glo luminescent cell viability assay (Promega, G7571) using a SpectraMax M5 Multi-Mode Microplate Reader (Molecular Devices) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Cells were seeded at a density of 50,000-100,000 cells per well in quadruplicate wells of a 96-well plate in medium supplemented with 0.5 mM beetle luciferin (Promega), and drugs were prepared in water and added at the concentrations indicated in the figures and legends ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK293T cells in 12-well plates were co-transfected with 0.05 µg each of GLP-1R-SmBiT and βarr2-LgBiT plasmid (Promega) plus 0.9 µg pcDNA3.1 ...
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bioRxiv - Synthetic Biology 2021Quote: Cells for were transferred to opaque-bottomed white plates (Grenier-Bio #655073) and then assessed for luminescence (Promega #E1501) per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were mixed by shaking the plates after which luminescence was recorded with a GloMax Explorer Model GM3510 (Promega) 10 min after adding the reagent.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with 50µL of luciferase assay reagent (Luciferase assay system, Promega). We immediately measured the luciferase activity with a Tecan SPARK multifunctional microplate reader (Tecan ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HeLa cells for 24 h (2 μg of cDNA/9.6 cm2 plate) using Fugene (Promega). For gene silencing ...
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bioRxiv - Molecular Biology 2022Quote: ... Fresh bacmid DNA was transfected into Sf9 cells at 0.5×106 cells/mL in 6-well cell culture plates using FuGene HD (Promega) according to the manufacturer’s protocol (final concentration 10 μg/mL) ...
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bioRxiv - Biophysics 2022Quote: Plates were left to equilibrate at room temperature for 20 minutes before addition of NanoGlo live cell substrate (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were then equilibrated to room temperature and lysed by the addition of Bright-Glo reagent (100 µL; Promega) to each well ...
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bioRxiv - Neuroscience 2022Quote: ... and placed in 96-well plates (white with a clear flat bottom) containing Nano-Glo Luciferase Assay Reagent (Promega) diluted 1000-fold in PBS at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Organoids from 2 wells of a 24 wells plate were collected in 500μl of cell lysis buffer and processed using RealiaPrep RNA Cell Miniprep System (Promega), according to manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with 50µL of luciferase assay reagent (Luciferase assay system, Promega). We immediately measured the luciferase activity with a Tecan SPARK multifunctional microplate reader (Tecan ...
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bioRxiv - Microbiology 2020Quote: ... The plate was incubated in the dark for 2 mins then read on a Glomax Multi+ Detection System (Promega) as above ...
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bioRxiv - Microbiology 2020Quote: ... The plate was incubated in the dark for 2 mins then read on a Glomax Multi+ Detection System (Promega) as above ...
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bioRxiv - Microbiology 2020Quote: ... 48 hours after transfection into 96 well plates in triplicates and cell number was measured by MTT assay (Promega) at 4 hours after addition of tetrazolium dye.
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bioRxiv - Cell Biology 2019Quote: ... cells were grown on Precision cover glass in 24-well plates or into glass bottom imaging dishes and transfected with 0.5 μg DNA construct complexed with Fugene6 (Promega) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The half maximal inhibitory concentrations (IC50 ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmids were recombined into 80,000 U-2 OS cells in a 24 well plate using 1.5 uL of FuGENE6 (Promega E2691) in 100 uL OPTIMEM (Fisher Scientific 31985070 ...
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bioRxiv - Biochemistry 2021Quote: ... per well of a 96-well plate 4×104 Drosophila SR2+ cells were seeded and transfected using Fugene (Promega) according to the manufacturer’s instructions with the following plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... The assay was carried out according to the manufacturer’s protocol for a 96-well format and measured using the GloMax Multi Plate Reader (Promega).
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bioRxiv - Microbiology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Immunology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was transferred into a fresh 384-well assay plate containing NanoGlo Luciferase Assay Substrate (Promega, Madison, WI), and luciferase intensity was measured by EnSpire Plate Reader (PerkinElmer ...
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bioRxiv - Biochemistry 2022Quote: ... the D10ACas9 nickase and the HDR template on 6-well plates at 70% confluency using FuGene® HD (Promega). Cells were allowed to recover for 7-10 days (splitting if necessary ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was assayed with CellTiter-Glo according to the manufacturer’s protocol using a Spectramax M5 plate reader (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... and the viability of the cancer cells was assessed by measuring luminescence with a GloMax Discover plate reader (Promega) or an IVIS Lumina III XR (Perkin-Elmer ...
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bioRxiv - Developmental Biology 2022Quote: ... into a 10 cm plate of HEK293T cells at 60-70% using Fugene 6 transfection reagent (Promega, Cat #E2691). Media containing lentiviral particles were collected at 24 ...
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bioRxiv - Genomics 2022Quote: MM001 and MM047 were seeded in 24-well plates and transfected with 400 ng pGL4.23-enhancer vector + 40 ng pRL-TK renilla vector (Promega) with Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples were then transferred to white 96-well plates and 50 μL of Bright-Glo Luciferase Assay Reagent (Promega) was added and mixed by manual orbital agitation ...